LOCUS       NM_001378247             939 bp    mRNA    linear   ROD 04-AUG-2024
DEFINITION  Mus musculus transmembrane protein 249 (Tmem249), mRNA.
ACCESSION   NM_001378247
VERSION     NM_001378247.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 939)
  AUTHORS   Huang,X., Miyata,H., Wang,H., Mori,G., Iida-Norita,R., Ikawa,M.,
            Percudani,R. and Chung,J.J.
  TITLE     A CUG-initiated CATSPERtheta; functions in the CatSper channel
            assembly and serves as a checkpoint for flagellar trafficking
  JOURNAL   Proc Natl Acad Sci U S A 120 (39), e2304409120 (2023)
   PUBMED   37725640
REFERENCE   2  (bases 1 to 939)
  AUTHORS   Huang,X., Miyata,H., Wang,H., Mori,G., Iida-Norita,R., Ikawa,M.,
            Percudani,R. and Chung,J.J.
  TITLE     A CUG-initiated CATSPERtheta; functions in the CatSper channel
            assembly and serves as a checkpoint for flagellar trafficking
  JOURNAL   bioRxiv (2023)
   PUBMED   36993167
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 939)
  AUTHORS   Lin,S., Ke,M., Zhang,Y., Yan,Z. and Wu,J.
  TITLE     Structure of a mammalian sperm cation channel complex
  JOURNAL   Nature 595 (7869), 746-750 (2021)
   PUBMED   34225353
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC157566.6.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR5189685.284800.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849384 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-103               AC157566.6         101390-101492
            104-257             AC157566.6         101567-101720
            258-352             AC157566.6         101931-102025
            353-548             AC157566.6         102131-102326
            549-634             AC157566.6         102493-102578
            635-939             AC157566.6         102872-103176
FEATURES             Location/Qualifiers
     source          1..939
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="15"
                     /map="15 36.03 cM"
     gene            1..939
                     /gene="Tmem249"
                     /gene_synonym="Catsperq; EG666504; Gm8140"
                     /note="transmembrane protein 249"
                     /db_xref="GeneID:666504"
                     /db_xref="MGI:MGI:3647471"
     exon            1..103
                     /gene="Tmem249"
                     /gene_synonym="Catsperq; EG666504; Gm8140"
                     /inference="alignment:Splign:2.1.0"
     exon            104..257
                     /gene="Tmem249"
                     /gene_synonym="Catsperq; EG666504; Gm8140"
                     /inference="alignment:Splign:2.1.0"
     exon            258..352
                     /gene="Tmem249"
                     /gene_synonym="Catsperq; EG666504; Gm8140"
                     /inference="alignment:Splign:2.1.0"
     CDS             302..817
                     /gene="Tmem249"
                     /gene_synonym="Catsperq; EG666504; Gm8140"
                     /note="Catsper theta"
                     /codon_start=1
                     /product="cation channel sperm-associated auxiliary
                     subunit TMEM249"
                     /protein_id="NP_001365176.1"
                     /db_xref="GeneID:666504"
                     /db_xref="MGI:MGI:3647471"
                     /translation="MLFIICLVFISCNVLREVKYQETWCFPAYGMVIGLWLMLSSIPQ
                     RRLVLNHTRGMYHFSIQGRTVCQGPMHLVYVRLALSSDAYGGRFFQLVLCGHKLEPLV
                     LVQLSERYEQMEFLGRHLARKLNINYFDYLASSYRHVVRHWPLGASFSPGIVQRKTQV
                     YTKSSVNDLDV"
     exon            353..548
                     /gene="Tmem249"
                     /gene_synonym="Catsperq; EG666504; Gm8140"
                     /inference="alignment:Splign:2.1.0"
     exon            549..634
                     /gene="Tmem249"
                     /gene_synonym="Catsperq; EG666504; Gm8140"
                     /inference="alignment:Splign:2.1.0"
     exon            635..939
                     /gene="Tmem249"
                     /gene_synonym="Catsperq; EG666504; Gm8140"
                     /inference="alignment:Splign:2.1.0"
     regulatory      917..922
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Tmem249"
                     /gene_synonym="Catsperq; EG666504; Gm8140"
                     /note="hexamer: ATTAAA"
     polyA_site      939
                     /gene="Tmem249"
                     /gene_synonym="Catsperq; EG666504; Gm8140"
                     /note="major polyA site"
ORIGIN      
        1 agaacccagg cagcccagcc tggactgctc cctgcttctc tgccctgctg ttgtctggcg
       61 gctcttgatt cttccctcag ctgcaaccct ggaattgtct cgggtcccca aggagcagca
      121 ggccagtggc tcccgcttct ccgcccttgt taagtacttc aaactctgga acgtaggact
      181 cttctctacc gagcgccact tagccaatca gctcaagaat aacagctttt accccttcaa
      241 gcagcaacag ccggatgtct ttgtgctcga gtactatttg gacacgctct ggaaggggac
      301 gatgctcttt atcatctgcc ttgtcttcat ttcctgtaat gtcctgagag aggtgaagta
      361 tcaggagacc tggtgctttc ctgcctatgg catggttata ggcttgtggc tcatgctctc
      421 ctcaataccg cagcgccgcc tagtgttgaa ccacacgcgc ggcatgtacc acttctccat
      481 ccaaggccga actgtatgtc aggggccaat gcatctggtc tacgtgcgcc tggcactcag
      541 ttctgatgcc tatggaggtc gcttcttcca gctggtcctt tgtggccaca agctagagcc
      601 acttgtgctg gtgcagctgt cagagcgcta tgagcaaatg gaattcttgg gccggcacct
      661 tgcccggaaa ctcaacatca actactttga ctacctggcc tcctcctatc gtcatgtagt
      721 ccgccactgg ccactggggg cctcctttag ccctggcatt gtgcagcgaa aaacacaagt
      781 ctacactaag tcaagtgtca acgacctgga tgtgtaaccc tggggcctga gccctggcct
      841 ccttttgcct accctgtgtc ccttatcccc atcctggagg gactaacagc cagccccgcc
      901 cactgccctt cctctaatta aagccacttt agcctctaa
//
