LOCUS       NM_030104                787 bp    mRNA    linear   ROD 04-JUN-2024
DEFINITION  Mus musculus transmembrane protein 247 (Tmem247), transcript
            variant 1, mRNA.
ACCESSION   NM_030104 XM_355035 XM_985719
VERSION     NM_030104.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 787)
  AUTHORS   Miyata,H., Castaneda,J.M., Fujihara,Y., Yu,Z., Archambeault,D.R.,
            Isotani,A., Kiyozumi,D., Kriseman,M.L., Mashiko,D., Matsumura,T.,
            Matzuk,R.M., Mori,M., Noda,T., Oji,A., Okabe,M.,
            Prunskaite-Hyyrylainen,R., Ramirez-Solis,R., Satouh,Y., Zhang,Q.,
            Ikawa,M. and Matzuk,M.M.
  TITLE     Genome engineering uncovers 54 evolutionarily conserved and
            testis-enriched genes that are not required for male fertility in
            mice
  JOURNAL   Proc Natl Acad Sci U S A 113 (28), 7704-7710 (2016)
   PUBMED   27357688
REFERENCE   2  (bases 1 to 787)
  AUTHORS   Choi,E., Lee,J., Oh,J., Park,I., Han,C., Yi,C., Kim,D.H., Cho,B.N.,
            Eddy,E.M. and Cho,C.
  TITLE     Integrative characterization of germ cell-specific genes from mouse
            spermatocyte UniGene library
  JOURNAL   BMC Genomics 8, 256 (2007)
   PUBMED   17662146
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 787)
  AUTHORS   Hong,S., Choi,I., Woo,J.M., Oh,J., Kim,T., Choi,E., Kim,T.W.,
            Jung,Y.K., Kim,D.H., Sun,C.H., Yi,G.S., Eddy,E.M. and Cho,C.
  TITLE     Identification and integrative analysis of 28 novel genes
            specifically expressed and developmentally regulated in murine
            spermatogenic cells
  JOURNAL   J Biol Chem 280 (9), 7685-7693 (2005)
   PUBMED   15613475
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC154492.2 and BC100486.1.
            
            On or before Feb 9, 2009 this sequence version replaced
            XM_985719.2, XM_355035.5.
            
            Transcript Variant: This variant (1) represents the shorter
            transcript and encodes the shorter isoform (1).
            
            Sequence Note: The stop codon annotated on this record is not found
            on the mouse reference genome. Instead, the first two nucleotides
            of the stop codon are encoded by the genome, and the third
            nucleotide is provided by the polyA tail. This unusual biology is
            supported by transcript alignments and orthologous data.
            
            ##Evidence-Data-START##
            RNAseq introns :: single sample supports all introns SAMN00849381,
                              SAMN00849384 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            polyA required for stop codon :: translation inferred
            RefSeq Select criteria        :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-86                AC154492.2         82389-82474
            87-787              BC100486.1         1-701
FEATURES             Location/Qualifiers
     source          1..787
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="17"
                     /map="17 56.9 cM"
     gene            1..787
                     /gene="Tmem247"
                     /gene_synonym="1700090G07Rik"
                     /note="transmembrane protein 247"
                     /db_xref="GeneID:78469"
                     /db_xref="MGI:MGI:1925719"
     exon            1..234
                     /gene="Tmem247"
                     /gene_synonym="1700090G07Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             118..753
                     /gene="Tmem247"
                     /gene_synonym="1700090G07Rik"
                     /note="isoform 1 is encoded by transcript variant 1;
                     transmembrane protein ENSP00000343375 homolog"
                     /codon_start=1
                     /product="transmembrane protein 247 isoform 1"
                     /protein_id="NP_084380.1"
                     /db_xref="CCDS:CCDS79579.1"
                     /db_xref="GeneID:78469"
                     /db_xref="MGI:MGI:1925719"
                     /translation="MAMEDREVMEARGAGESCPTLSKVAPVDSMPEGKPKASLDAEVP
                     KLELPTLEENGICEDRDCPGPPRSLPPKSGPNAKGQAGDGPGLESVELPLPLETEHRN
                     AMELEKVRMEFELTLLKYLHQENERQRQHEEVMEQLQQQQQQQQALPHQFSGSLQDLL
                     LPQNQFAMFFYCFIFIHIIYVAKETVFFLFSKHYLFCLAAILLCLIKTLWS"
     exon            235..570
                     /gene="Tmem247"
                     /gene_synonym="1700090G07Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            571..752
                     /gene="Tmem247"
                     /gene_synonym="1700090G07Rik"
                     /inference="alignment:Splign:2.1.0"
     regulatory      733..738
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Tmem247"
                     /gene_synonym="1700090G07Rik"
                     /note="hexamer: ATTAAA"
     polyA_site      752
                     /gene="Tmem247"
                     /gene_synonym="1700090G07Rik"
                     /note="major polyA site"
ORIGIN      
        1 ggaatgtggc cattatgagg atagggattg tgatggtggt cagaacctgg tgaagccaaa
       61 tggtattgtc ttaaaagatg tcctgtggcc ctggtccgca gcacggtggt tatctggatg
      121 gcgatggagg acagggaggt gatggaagcc cgaggggcag gagaaagctg ccccacactc
      181 tccaaggtgg cccctgttga cagcatgcct gaaggcaagc caaaggcctc tttggatgca
      241 gaagtcccca aactagaact gcctaccctg gaagagaacg ggatctgtga ggacagagat
      301 tgtccggggc cccctaggtc attgcctcca aagtccggcc caaacgccaa gggtcaggct
      361 ggcgatggac ctggactgga gtccgtagag ctgcccctgc ccctggagac agaacaccgc
      421 aacgcgatgg agctggagaa ggtgcgcatg gagtttgaac tgacgctcct caagtacctg
      481 caccaggaga acgagcggca gaggcagcat gaggaagtga tggagcagct gcaacagcag
      541 cagcagcagc agcaggcttt gcctcaccag ttctcaggaa gcctccagga cctcttgctc
      601 ccccagaacc aattcgccat gttcttctac tgcttcatct tcatccacat catctacgtt
      661 gccaaggaga cggtcttctt tctcttctcc aagcactacc tgttctgtct ggcagccatc
      721 ttgctgtgct tgattaaaac cttgtggtcc taaaaaaaaa aaaaaaaaaa aaaaaaaaaa
      781 aaaaaaa
//
