LOCUS       NM_029379                993 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus transmembrane protein 225 (Tmem225), transcript
            variant 1, mRNA.
ACCESSION   NM_029379 XM_134639 XM_916123
VERSION     NM_029379.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 993)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 993)
  AUTHORS   Lv,Z., Sun,L., Xie,X., Yao,X., Tian,S., Wang,C., Wang,F. and Liu,J.
  TITLE     TMEM225 Is Essential for Sperm Maturation and Male Fertility by
            Modifying Protein Distribution of Sperm in Mice
  JOURNAL   Mol Cell Proteomics 23 (2), 100720 (2024)
   PUBMED   38246484
  REMARK    GeneRIF: TMEM225 Is Essential for Sperm Maturation and Male
            Fertility by Modifying Protein Distribution of Sperm in Mice.
REFERENCE   3  (bases 1 to 993)
  AUTHORS   Matsuura,M. and Yogo,K.
  TITLE     TMEM225: a possible protein phosphatase 1gamma2 (PP1gamma2)
            regulator localizes to the equatorial segment in mouse spermatozoa
  JOURNAL   Mol Reprod Dev 82 (2), 139-148 (2015)
   PUBMED   25605614
  REMARK    GeneRIF: TMEM225 is involved in the differentiation and function of
            spermatozoa through the regulation of PP1gamma2 activity.
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK006538.1 and BX630292.1.
            
            On or before Jul 25, 2009 this sequence version replaced
            XM_916123.2, XM_134639.3.
            
            Transcript Variant: This variant (1) encodes the longer isoform
            (1).
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK006538.1, BC116869.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849384 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-987               AK006538.1         2-988
            988-993             BX630292.1         1-6                 c
FEATURES             Location/Qualifiers
     source          1..993
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="9"
                     /map="9 21.42 cM"
     gene            1..993
                     /gene="Tmem225"
                     /gene_synonym="1700030E15Rik; Tnen225"
                     /note="transmembrane protein 225"
                     /db_xref="GeneID:75667"
                     /db_xref="MGI:MGI:1922917"
     exon            1..385
                     /gene="Tmem225"
                     /gene_synonym="1700030E15Rik; Tnen225"
                     /inference="alignment:Splign:2.1.0"
     CDS             205..897
                     /gene="Tmem225"
                     /gene_synonym="1700030E15Rik; Tnen225"
                     /note="isoform 1 is encoded by transcript variant 1"
                     /codon_start=1
                     /product="transmembrane protein 225 isoform 1"
                     /protein_id="NP_083655.1"
                     /db_xref="CCDS:CCDS52774.1"
                     /db_xref="GeneID:75667"
                     /db_xref="MGI:MGI:1922917"
                     /translation="MMHIPNRSIQAANIFFSSGAILLLIVGLIMEDWVELIPKVRKDK
                     TTHSPWLGCCPPFWPEESLEVVRRIMRMTLNISIYLNLIIGLQFSYMISQNKCVHLLV
                     GFLSFFAGCLLFYAIIVYHHKLNKGQYVYFVNYKTKWIAFTVYLTIALFLTCGIFCFI
                     QSTNRCECMKFCIPHTESKSQEMIPSTIEVVSLPPRCAMPRSIVHVHSVTSKDGSLNR
                     PHTQARRVTWAL"
     exon            386..532
                     /gene="Tmem225"
                     /gene_synonym="1700030E15Rik; Tnen225"
                     /inference="alignment:Splign:2.1.0"
     exon            533..667
                     /gene="Tmem225"
                     /gene_synonym="1700030E15Rik; Tnen225"
                     /inference="alignment:Splign:2.1.0"
     exon            668..993
                     /gene="Tmem225"
                     /gene_synonym="1700030E15Rik; Tnen225"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 agtctgccct ttatataaga atggtaaagg gaggaagtta tcttttctgt tcttcagcta
       61 acttcaactt ctgcctctac tgatgcctct gccaacatct ctgcagtcag tgaacatcca
      121 gagttcattc tgcatcttct gactcatcaa atcaaagacg tgtaaaacat tggtggtagt
      181 ctctggtaga aatttttatc cacaatgatg cacatcccaa acagaagtat ccaggctgcc
      241 aacatattct tctcctctgg tgccatactt ctgttgatag taggactcat catggaagac
      301 tgggtggaac tgattcccaa agtgagaaag gataaaacta ctcacagtcc atggctggga
      361 tgctgtcctc ctttttggcc cgaagagagc ctggaggtgg tcaggcggat aatgaggatg
      421 actctcaaca tttccatcta tctaaacttg atcataggtt tgcagttctc ctatatgatt
      481 tctcaaaaca agtgtgtgca tctccttgtt ggcttcctga gtttcttcgc aggttgcctt
      541 ttgttctatg cgatcattgt gtatcaccac aagctaaata aaggtcaata tgtgtacttc
      601 gttaattaca agaccaagtg gatagctttc actgtctact taacaatcgc cctctttctt
      661 acctgtggta tcttctgttt catacagagc acaaatagat gcgaatgcat gaaattctgt
      721 ataccccaca cagaaagtaa aagccaagaa atgattccga gtacaatcga agttgtttca
      781 cttccgccac gatgtgcaat gcctcgaagc atcgtccatg tgcactctgt gacctcaaag
      841 gatggctcct taaatagacc acacacccaa gcacgtcggg taacctgggc tctgtgactt
      901 gacaatacgt acctttcagt actacctgac gttgctatgg aattagctgt atgtaaccaa
      961 tcatttgtgt atggcaataa aaagttcaga aaa
//
