LOCUS       NM_026798               1101 bp    mRNA    linear   ROD 05-JUN-2024
DEFINITION  Mus musculus transmembrane protein 216 (Tmem216), transcript
            variant 2, mRNA.
ACCESSION   NM_026798
VERSION     NM_026798.3
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1101)
  AUTHORS   Wang,Y., Yao,H., Zhang,Y., Mu,N., Lu,T., Du,Z., Wu,Y., Li,X.,
            Su,M., Shao,M., Sun,X., Su,L. and Liu,X.
  TITLE     TMEM216 promotes primary ciliogenesis and Hedgehog signaling
            through the SUFU-GLI2/GLI3 axis
  JOURNAL   Sci Signal 17 (820), eabo0465 (2024)
   PUBMED   38261656
  REMARK    GeneRIF: TMEM216 promotes primary ciliogenesis and Hedgehog
            signaling through the SUFU-GLI2/GLI3 axis.
REFERENCE   2  (bases 1 to 1101)
  AUTHORS   Garcia-Gonzalo,F.R., Corbit,K.C., Sirerol-Piquer,M.S.,
            Ramaswami,G., Otto,E.A., Noriega,T.R., Seol,A.D., Robinson,J.F.,
            Bennett,C.L., Josifova,D.J., Garcia-Verdugo,J.M., Katsanis,N.,
            Hildebrandt,F. and Reiter,J.F.
  TITLE     A transition zone complex regulates mammalian ciliogenesis and
            ciliary membrane composition
  JOURNAL   Nat Genet 43 (8), 776-784 (2011)
   PUBMED   21725307
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 1101)
  AUTHORS   Nelson,P.S., Pritchard,C., Abbott,D. and Clegg,N.
  TITLE     The human (PEDB) and mouse (mPEDB) Prostate Expression Databases
  JOURNAL   Nucleic Acids Res 30 (1), 218-220 (2002)
   PUBMED   11752298
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BY704000.1, AK003740.1 and
            BX633507.1.
            
            On Apr 24, 2013 this sequence version replaced NM_026798.2.
            
            Summary: This gene encodes a transmembrane protein which is
            involved in regulation of signaling and trafficking of associated
            proteins. In humans, mutations in this gene are associated with
            ciliopathies including Joubert, Meckel and related syndromes.
            Alternative splicing of this gene results in multiple transcript
            variants encoding different isoforms. [provided by RefSeq, Apr
            2013].
            
            Transcript Variant: This variant (2) contains an alternate 5'
            terminal exon, differs in the 5' UTR, lacks a portion of the 5'
            coding region, and initiates translation at a downstream start
            codon, compared to variant 1. Variants 2 and 3 encode the same
            isoform (2), which has a shorter N-terminus compared to isoform 1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK003740.1, SRR1660821.142648.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164137 [ECO:0000348]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-727               BY704000.1         3-729
            728-1096            AK003740.1         730-1098
            1097-1101           BX633507.1         3-7                 c
FEATURES             Location/Qualifiers
     source          1..1101
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="19"
                     /map="19 6.6 cM"
     gene            1..1101
                     /gene="Tmem216"
                     /gene_synonym="1110017C22Rik; 2810441K11Rik;
                     4921533J23Rik; A930021F15Rik"
                     /note="transmembrane protein 216"
                     /db_xref="GeneID:68642"
                     /db_xref="MGI:MGI:1920020"
     exon            1..124
                     /gene="Tmem216"
                     /gene_synonym="1110017C22Rik; 2810441K11Rik;
                     4921533J23Rik; A930021F15Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            125..226
                     /gene="Tmem216"
                     /gene_synonym="1110017C22Rik; 2810441K11Rik;
                     4921533J23Rik; A930021F15Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            227..319
                     /gene="Tmem216"
                     /gene_synonym="1110017C22Rik; 2810441K11Rik;
                     4921533J23Rik; A930021F15Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             274..537
                     /gene="Tmem216"
                     /gene_synonym="1110017C22Rik; 2810441K11Rik;
                     4921533J23Rik; A930021F15Rik"
                     /note="isoform 2 is encoded by transcript variant 2;
                     thymus atrophy-related protein"
                     /codon_start=1
                     /product="transmembrane protein 216 isoform 2"
                     /protein_id="NP_081074.1"
                     /db_xref="CCDS:CCDS37913.1"
                     /db_xref="GeneID:68642"
                     /db_xref="MGI:MGI:1920020"
                     /translation="MLLLYLGIEVIRLFFGTKGNLCQRKMPLGISVALTFPSAMMASY
                     YLLLQTYVLRLEAIMNSILLFFCGSELLLEMLTLATFSSMDRI"
     exon            320..521
                     /gene="Tmem216"
                     /gene_synonym="1110017C22Rik; 2810441K11Rik;
                     4921533J23Rik; A930021F15Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            522..1101
                     /gene="Tmem216"
                     /gene_synonym="1110017C22Rik; 2810441K11Rik;
                     4921533J23Rik; A930021F15Rik"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1080..1085
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Tmem216"
                     /gene_synonym="1110017C22Rik; 2810441K11Rik;
                     4921533J23Rik; A930021F15Rik"
                     /note="hexamer: AATAAA"
     polyA_site      1099
                     /gene="Tmem216"
                     /gene_synonym="1110017C22Rik; 2810441K11Rik;
                     4921533J23Rik; A930021F15Rik"
                     /note="major polyA site"
ORIGIN      
        1 tcctttcaac acccttgcac tctttgggtc gcaggagaat ctcattatag caccgctgtt
       61 ttcccttgta ctcctagaaa ggacattcta tcttcaacgg ttttggtgtt tacataggct
      121 ccagataaaa gattgtcctc aacccccctg gaagtcctgt tctttctgaa tgggtggtac
      181 tatgctacct atttcctgct ggagctcctt attttcctgt acaaagggct cctgttgccg
      241 tacccaacag ccaacttagt cctggatgtg gtgatgctgc tgctctatct tggcattgaa
      301 gtcatacgat tgtttttcgg tacaaaggga aacctctgcc agcgaaagat gccccttggc
      361 attagtgtgg ccttgacctt cccatccgct atgatggctt cctattacct gctgctgcag
      421 acctacgtgc tccgcctaga agctatcatg aacagtatct tgctcttctt ttgtggctca
      481 gagctgctgc ttgagatgct cacgctggcc accttctcca gcatggacag gatctgaagt
      541 acaagaattc cagctggtga caacacatat ccatctggta cttcagccac actggtgaaa
      601 agtggtaggt cgagctaacc tgggaaaggt ttgtgtggtt tttcctcagt gcggacattt
      661 gggcgctgag cacctacctg aggccagtgg caccatcttt aaagcgggat catcagcagg
      721 agactctttc cttttgttgt agggaggggc aaggctgtcc ccatcttggt gtttctaaca
      781 gcttcctaat gatctcaagt tctcttctgt ggtcatcatg gccagagcac cttgtgtgga
      841 ccttctagtt ttgtttggct tccagcagaa cctgagccac agcactctgt gcacgtacat
      901 ctgtcctaca tgaccatgga gagcctcata atggtgggct cttgggctga ttccaatgtg
      961 tgttctccac gctgctccac ctgcccccat cctgctcaaa gagatggtga tctagaaccc
     1021 atttagtagt ttatagtagt atttttctat tctgatcatg aagcaaacac aaaattttta
     1081 ataaaagtat tttctgatac a
//
