LOCUS       NM_001379146            3631 bp    mRNA    linear   ROD 09-JUN-2024
DEFINITION  Mus musculus transmembrane protein 196 (Tmem196), transcript
            variant 4, mRNA.
ACCESSION   NM_001379146 XM_006515787
VERSION     NM_001379146.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3631)
  AUTHORS   Chen,J., Wang,D., Chen,H., Gu,J., Jiang,X., Han,F., Cao,J., Liu,W.
            and Liu,J.
  TITLE     TMEM196 inhibits lung cancer metastasis by regulating the
            Wnt/beta-catenin signaling pathway
  JOURNAL   J Cancer Res Clin Oncol 149 (2), 653-667 (2023)
   PUBMED   36355209
  REMARK    GeneRIF: TMEM196 inhibits lung cancer metastasis by regulating the
            Wnt/beta-catenin signaling pathway.
REFERENCE   2  (bases 1 to 3631)
  AUTHORS   Thompson,C.L., Ng,L., Menon,V., Martinez,S., Lee,C.K.,
            Glattfelder,K., Sunkin,S.M., Henry,A., Lau,C., Dang,C.,
            Garcia-Lopez,R., Martinez-Ferre,A., Pombero,A., Rubenstein,J.L.R.,
            Wakeman,W.B., Hohmann,J., Dee,N., Sodt,A.J., Young,R., Smith,K.,
            Nguyen,T.N., Kidney,J., Kuan,L., Jeromin,A., Kaykas,A., Miller,J.,
            Page,D., Orta,G., Bernard,A., Riley,Z., Smith,S., Wohnoutka,P.,
            Hawrylycz,M.J., Puelles,L. and Jones,A.R.
  TITLE     A high-resolution spatiotemporal atlas of gene expression of the
            developing mouse brain
  JOURNAL   Neuron 83 (2), 309-323 (2014)
   PUBMED   24952961
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC164110.4 and AC225445.3.
            
            On Mar 25, 2020 this sequence version replaced XM_006515787.3.
            
            Transcript Variant: This variant (4) represents the longest
            transcript and encodes the longest isoform (4).
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660825.99446.1,
                                           ERR3363660.1949767.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164131, SAMN01164132
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-512               AC164110.4         128120-128631
            513-569             AC164110.4         193241-193297
            570-824             AC164110.4         196741-196995
            825-898             AC164110.4         198126-198199
            899-1446            AC164110.4         200503-201050
            1447-3631           AC225445.3         7781-9965           c
FEATURES             Location/Qualifiers
     source          1..3631
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="12"
                     /map="12 63.86 cM"
     gene            1..3631
                     /gene="Tmem196"
                     /gene_synonym="Gm528"
                     /note="transmembrane protein 196"
                     /db_xref="GeneID:217951"
                     /db_xref="MGI:MGI:2685374"
     exon            1..512
                     /gene="Tmem196"
                     /gene_synonym="Gm528"
                     /inference="alignment:Splign:2.1.0"
     CDS             348..905
                     /gene="Tmem196"
                     /gene_synonym="Gm528"
                     /note="isoform 4 is encoded by transcript variant 4"
                     /codon_start=1
                     /product="transmembrane protein 196 isoform 4"
                     /protein_id="NP_001366075.1"
                     /db_xref="GeneID:217951"
                     /db_xref="MGI:MGI:2685374"
                     /translation="MCTSGQIIGSLLVLSVLEIGLGVSSVAVGAVSFSLALREHTPQL
                     GDSSPVWSGVCFLLCGICGILCAKKKSGLVMILFSACCICGLIGGILNFQFLRAVTKK
                     TSSLYPLHLASMSLACIGIGGCTVSSWLTCRLASYEQRRMFSEREHSLHHSHEMAEKR
                     LRAIEITDLPSCPVVPPTPELPTRK"
     exon            513..569
                     /gene="Tmem196"
                     /gene_synonym="Gm528"
                     /inference="alignment:Splign:2.1.0"
     exon            570..824
                     /gene="Tmem196"
                     /gene_synonym="Gm528"
                     /inference="alignment:Splign:2.1.0"
     exon            825..898
                     /gene="Tmem196"
                     /gene_synonym="Gm528"
                     /inference="alignment:Splign:2.1.0"
     exon            899..3631
                     /gene="Tmem196"
                     /gene_synonym="Gm528"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3607..3612
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Tmem196"
                     /gene_synonym="Gm528"
                     /note="hexamer: AATAAA"
     polyA_site      3631
                     /gene="Tmem196"
                     /gene_synonym="Gm528"
                     /note="major polyA site"
ORIGIN      
        1 agaactctgg actgcgcctc cgacactggc aaggcttccg atccagagtc cgttctccag
       61 agcctggctc ctcaataaaa gcttctggac tcccacccat cgctcaacag tccctgtgtg
      121 aacccgccac gggctggatt agctgtcttc tctggccagg cccacggaaa tggttctctg
      181 gttctgaaaa tccagacctt gtggataatg ttttgctggt gaagaaagaa aagtttgagg
      241 agctggactg tggtaggggg cggaaggggc tgaggaggac tttggaagaa agaaggtaga
      301 tgatcagctt tcatctctga gctggaagga atgatgaccc aaggagaatg tgcaccagcg
      361 gccagatcat tgggagcctt ttggtgctct ctgtgctgga gatagggctg ggggtgtcca
      421 gcgtggccgt gggggcggtc agcttcagcc tggctctccg agagcacacg ccgcagctcg
      481 gagactcgtc cccggtatgg agcggggtgt gttttcttct atgtggcata tgtggaatac
      541 tgtgcgccaa aaaaaagtca ggacttgtca tgatcctctt ttcagcgtgc tgtatctgtg
      601 gactcattgg aggcatcctg aattttcagt ttcttcgggc agtcacaaaa aagacctcct
      661 ccctgtaccc actgcatctc gcttccatgt ctttggcatg tatcggcatt gggggctgca
      721 ctgtctcctc ttggctcact tgtcgtctag ccagctatga gcaaaggagg atgttctcag
      781 aaagggaaca ttccctgcat cactctcatg aaatggctga gaaaagattg agggctattg
      841 aaataaccga cttgcccagc tgcccggtgg tgcccccgac accagagtta cctacaagga
      901 aatgacagac aacatgagta atggagcacc gcaactgatg tttaatggaa gagtacaatc
      961 aatgttttaa agaactcaac atttttcagg ttgttcatga ggcaggagaa gctgaagacc
     1021 tgggagtagc agttaaaatg gctcttggat ttcttatttc tcctgggcat cgactaaagt
     1081 tattccttct cagtttggcc tcactcaccc tttatccctt tctagagggt ataaactttc
     1141 cagaaatgtt gtagcttagt catctcagca tactgaccaa tttttatgaa gaagattcct
     1201 ccaaaaaact aaatggaaat agcagtgact aatactgtac acagacacca tttgactagc
     1261 atgagcttta gaattgtaat tgcatatttt atgaatcatt caaaagtaaa cacatacaga
     1321 accaacaata acttattgat gtctttgttt tgaaagacaa atgatttcaa gtatgctaaa
     1381 aatcaagacc tcagcaattc cttgttgcaa caacattgta attaatttca atttgtgaat
     1441 gaattctatt tcataattta tttataaatt acaaaaaact aaagcttaaa aggtgatttt
     1501 tttagttaga gaaagaatac taatctgtat gatatttttg cacttttgaa caagtgaata
     1561 ctatgtaagg aaaataaatg ttttcattat tctgtgcaaa ttgatcttca tgttcatgaa
     1621 tgagtctaag aataaagtat tgtgtataca taggtgtata tatactgtaa atacagaaaa
     1681 tacacacatc atatatttat agctacatga atgtaaatag gtaataggga agaaattgta
     1741 atgttatgaa gttattccac tagctatgag aaggccattg ctcaaagctt tctaagccca
     1801 caatgtcaca tatttaattt ctactctatc catgcatttt cttctttttc ttaaatctat
     1861 tgtgtggtag acaagcattg tggctgatac atatggagct gagtgacttg aatattgaag
     1921 catgaatata aactacttaa ttgttggtat ttaactgtga ttatgatgtt tatgtaaaag
     1981 taattgttaa tattgattta taagtgtaac ttcaatcata taaatcctta tttctaaaca
     2041 aaaattatta tctaagatta ttcctgatat gtttatttca tataactttc agccaaatta
     2101 agctctgtaa ttgggaaatg gtaaccttag tgaagttaat actacctttg ctatacttta
     2161 gatgttttat ctcctttgct gattaacatg aaggtcatat gttggaaagt atatattcaa
     2221 agtatctgaa ggagttctca gtataagaaa attgaaagat aaactgacat tcacatagtt
     2281 aaagccaatt ttcctcttcc acaatgcaat attaaaataa taatcaacaa tatgtataaa
     2341 agatcaaaca tcatggactc taaagatatt tatgaaaatt aatctctgac aacatacaag
     2401 tcaatatttt taaataagta gcattgcacc atatattttt attaatttcc aaaactaagc
     2461 aaatttcaac tcatttttct gagttactca atcacagcta aattgatata tttcatgctt
     2521 ggtatgagaa caccttacag tcacaagcaa gacaagagtt ttcactgtgc taacaaaaca
     2581 gcaataattt ggtctaaggc cccatgagcc tttaacaatg tcatcagtaa acctgttgag
     2641 atgaactact tagaaaattc tggataaaaa tggcctctta ttttggtata gttttgaact
     2701 cttcctcact catttggaca cttctttgtc tggtatatta caaatactgt ccatagattt
     2761 gatataaaat aatgaatcat catcatcact actcatttca tgctgcatat aggcatgata
     2821 tttgccacta aaaatgcaaa ctctaagcac ctagaaatct caaatatata tggttatact
     2881 aaatttatgc tgaagttggc ttaatcttta aggaagaaac ccaccgactc aaattttggt
     2941 agcatttctc tgttggcatg taaaatacag aattataata tatcaacatg tttagtgact
     3001 tttgaattta tttataaaaa cacatattaa aaaaccaaag aaggctatca cttctttgac
     3061 acacagattt tttaaaaaga aattcaatca ctgcacttat cttaatagtt cacagtgtgt
     3121 gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt atgtgtgtgt gtgtgggtgt gtgggtgtgt
     3181 gggtgtgtgt atgtgtataa tcaattttag acacatcttt aaatgtaatt atttcaccct
     3241 tgtgctttct cagctcaaat tggagtgatt tacttagggt ataagatgat tcttgaaata
     3301 agtaggggaa gttaagaaga cattattcaa gaaactgaaa ttttaatttt tacaacaaac
     3361 tgacaaagaa taaaaacatt agtgcctaca ttatagtttt aatataaatc tacccttgat
     3421 gaaatatgat gtggtattaa attaggaaaa tatatgtgaa agtggtgtgg ggtatgcttt
     3481 aaatgcaaaa agaagacgct atcacttcta ggaaattaaa aaaaatattt gtatgtatat
     3541 atatgtatta tatacacaca tatgttttga agtggaacat tgtatgatga ctcttgatgt
     3601 ttatgtaata aaaccatatg ctttgaaact a
//
