LOCUS       NM_001357750            2513 bp    mRNA    linear   ROD 02-MAY-2024
DEFINITION  Mus musculus transmembrane protein 185A (Tmem185a), transcript
            variant 1, mRNA.
ACCESSION   NM_001357750 XM_006527981
VERSION     NM_001357750.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2513)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 2513)
  AUTHORS   Ingham,N.J., Pearson,S.A., Vancollie,V.E., Rook,V., Lewis,M.A.,
            Chen,J., Buniello,A., Martelletti,E., Preite,L., Lam,C.C.,
            Weiss,F.D., Powis,Z., Suwannarat,P., Lelliott,C.J., Dawson,S.J.,
            White,J.K. and Steel,K.P.
  TITLE     Mouse screen reveals multiple new genes underlying mouse and human
            hearing loss
  JOURNAL   PLoS Biol 17 (4), e3000194 (2019)
   PUBMED   30973865
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2513)
  AUTHORS   Leduc,M.S., Savage,H.S., Stearns,T.M., Cario,C.L., Walsh,K.A.,
            Paigen,B. and Berndt,A.
  TITLE     A major X-linked locus affects kidney function in mice
  JOURNAL   Mol Genet Genomics 287 (11-12), 845-854 (2012)
   PUBMED   23011808
REFERENCE   4  (bases 1 to 2513)
  AUTHORS   Maurer,M.H., Grunewald,S., Gassler,N., Rossner,M., Propst,F.,
            Wurz,R., Weber,D., Kuner,T., Kuschinsky,W. and Schneider,A.
  TITLE     Cloning of a novel neuronally expressed orphan G-protein-coupled
            receptor which is up-regulated by erythropoietin, interacts with
            microtubule-associated protein 1b and colocalizes with the
            5-hydroxytryptamine 2a receptor
  JOURNAL   J Neurochem 91 (4), 1007-1017 (2004)
   PUBMED   15525354
  REMARK    GeneRIF: The mouse ee3 is an orphan G-protein-coupled receptor with
            potential connections to erythropoietin and 5HT2a receptor
            signalling. ee3_2, a similar gene, is also described, along with
            human orthologs.
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL672026.11 and BC145368.1.
            
            On Nov 10, 2017 this sequence version replaced XM_006527981.2.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC145368.1, SRR7345562.779292.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164134 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-141               AL672026.11        97756-97896         c
            142-1614            BC145368.1         1-1473
            1615-2513           AL672026.11        80469-81367         c
FEATURES             Location/Qualifiers
     source          1..2513
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="X"
                     /map="X 36.04 cM"
     gene            1..2513
                     /gene="Tmem185a"
                     /gene_synonym="ee3"
                     /note="transmembrane protein 185A"
                     /db_xref="GeneID:236848"
                     /db_xref="MGI:MGI:2448555"
     exon            1..203
                     /gene="Tmem185a"
                     /gene_synonym="ee3"
                     /inference="alignment:Splign:2.1.0"
     CDS             166..1218
                     /gene="Tmem185a"
                     /gene_synonym="ee3"
                     /note="isoform 1 is encoded by transcript variant 1;
                     erythropoietin-induced EST 3"
                     /codon_start=1
                     /product="transmembrane protein 185A isoform 1"
                     /protein_id="NP_001344679.1"
                     /db_xref="CCDS:CCDS90721.1"
                     /db_xref="GeneID:236848"
                     /db_xref="MGI:MGI:2448555"
                     /translation="MNLRGLFQDFNPSKFLIYACLLLFSVLLALRLDGIIQWSYWAVF
                     APIWLWKLMVIVGASVGTGVWARNPQYRAEGETCVEFKAMLIAVGIHLLLLMFEVLVC
                     DRIERGSHFWLLVFMPLFFVSPVSVAACVWGFRHDRSLELEILCSVNILQFIFIALRL
                     DKIIHWPWLVVCVPLWILMSFLCLVVLYYIVWSVLFLRSMDVIAEQRRTHITMALSWM
                     TIVVPLLTFEILLVHKLDGHNAFSCIPIFVPLWLSLITLMATTFGQKGGNHWWFGIRK
                     DFCQFLLEIFPFLREYGNISYDLHHEDSEETEETPVPEPPKIAPMFRKKARVVITQSP
                     GKYVLPPPKLNIEMPD"
     exon            204..380
                     /gene="Tmem185a"
                     /gene_synonym="ee3"
                     /inference="alignment:Splign:2.1.0"
     exon            381..588
                     /gene="Tmem185a"
                     /gene_synonym="ee3"
                     /inference="alignment:Splign:2.1.0"
     exon            589..672
                     /gene="Tmem185a"
                     /gene_synonym="ee3"
                     /inference="alignment:Splign:2.1.0"
     exon            673..849
                     /gene="Tmem185a"
                     /gene_synonym="ee3"
                     /inference="alignment:Splign:2.1.0"
     exon            850..973
                     /gene="Tmem185a"
                     /gene_synonym="ee3"
                     /inference="alignment:Splign:2.1.0"
     exon            974..2513
                     /gene="Tmem185a"
                     /gene_synonym="ee3"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2489..2494
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Tmem185a"
                     /gene_synonym="ee3"
                     /note="hexamer: AATAAA"
     polyA_site      2513
                     /gene="Tmem185a"
                     /gene_synonym="ee3"
                     /note="major polyA site"
ORIGIN      
        1 acggaagccg gaaggagtgt gggagacagt tggttgctct ggtttctcct ggcggcggcg
       61 ttagcgacga agctgccttt ggcagcatga acccgctgtg aggcgcaggc ttcgcggcgc
      121 ggggggcggg gggaagagga ggacgcctcg gcgaagttct ccgccatgaa cctgaggggc
      181 ctctttcagg acttcaaccc gagtaaattc ctcatctatg cctgtctgct gctgttctct
      241 gtgctactgg cccttcgttt ggatggcatc attcaatgga gctactgggc tgtctttgct
      301 ccaatatggt tgtggaagtt aatggtcatt gttggcgcat cagttggaac tggagtttgg
      361 gcacgaaatc ctcagtatcg agcagaagga gaaacatgtg tggagtttaa agccatgtta
      421 attgctgtgg gcatccattt gctcctgctg atgtttgaag ttctggtctg tgacaggatt
      481 gagagaggaa gccatttctg gcttctggtc ttcatgccat tgttctttgt ttccccagtg
      541 tctgttgcag catgtgtctg gggctttcga catgacaggt cactagagct agaaatcctg
      601 tgttcggtca acattcttca gtttatattc attgccttaa gactggacaa gattatccac
      661 tggccctggc ttgttgtgtg tgtccctctg tggattctca tgtccttctt gtgcctggtg
      721 gtcctctatt acatcgtttg gtcggttctg ttcctgcgct ctatggatgt gattgcagaa
      781 caacgcagaa cacatataac catggcactg agctggatga ccattgtcgt gcctcttctt
      841 acttttgaga tcctgctggt tcacaaactg gatggccaca atgctttctc ctgtattcca
      901 atatttgttc ccctgtggct ctcattgatc acactgatgg caacaacatt tggacagaaa
      961 ggaggaaacc actggtggtt tggtattcgc aaagacttct gccagtttct gcttgaaata
     1021 ttcccgtttt tgagagagta tggaaacatt tcatatgatc tccaccatga agatagtgag
     1081 gaaacagaag aaaccccagt tccagaacct cctaaaattg cacctatgtt ccgcaagaag
     1141 gccagggtag tcatcaccca gagccctggc aagtatgtcc ttcctccacc caaactaaat
     1201 attgaaatgc cagactaaag gacgccacca gggccgccgg agtgtgtgtg cactgagaca
     1261 cacactccta cttcccttgc ccccaaccta gacctggaac ctcggaccac catctgaagc
     1321 ctcgtttgca tccagtgtca accagccact cagaacagaa cataggtggc agggactaag
     1381 ggtatcttat caatgtggat gccagtgaca catgagcaaa gaacctattt ctttcgtatg
     1441 ggtacctgaa atgtgttctc attcctgtga tacccaccca gggatgactg ctgcctaggc
     1501 ccacagaaag cacatctgtg gtctgggcag attttctagt gctaaaattc ctatcaaagt
     1561 attgaatgat gtggttcctc tgggaccact tccatctgtg gctcagctgt gctgcttatg
     1621 ggtagccagt agggactata agtaggtctg tacacacctg ctgtgttcag atacagcccg
     1681 gccctgcttt ccttgtctct gctgtacaga aatttagctt tagccaggat gacagcaggt
     1741 ttgcagggcc tcatcagtga gcccttccca atggcatgat tccatgccta tatagtattt
     1801 atacagctat tttagcactg taatatacag tgtcaaatcc tagttctgta cagcatattc
     1861 tctgttaaag tattttttta caagcttgtg ctggagatgg atgggttaag ccatgggaga
     1921 agcagatagc tcagactgct gtatctcagt cagatgcccc acctcactta ccatgcccac
     1981 tgataccctg gaatttctgc cagcacagct actacaatca aggaaagctc tgtgcttttc
     2041 caacatgggg tgtgggagaa atggcttaaa agcccaagaa atgccccctt gagaccagga
     2101 cactagagaa ggttgccaag gcaggccaga atcagccagg aactcctgca aagtcgagag
     2161 cattctgcct acctgcaaga agaacactgc atactgggca tgcaggtacc accacctgtc
     2221 agcagtttct ccaggctctg ctggtatagc cagcccagaa cccatcatac cagaaaataa
     2281 ccttcagtct aaatcaaatg attcattcat ggtcacctga tcagtggctt cagtcacaca
     2341 taattgaagt ctctaaccct agtcctaccc ttggcacagg cctccagtga gcctgttctg
     2401 tttcagaaga aaggggaagc agtgtagcat tttttgtttg gtctcttaaa aatgtggtta
     2461 aatccactat tttttgctcc tgtagttcaa taaagtggtt cattttacat gta
//
