LOCUS       NM_172049               2971 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus transmembrane protein 18 (Tmem18), mRNA.
ACCESSION   NM_172049 XM_910002 XM_921502
VERSION     NM_172049.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2971)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 2971)
  AUTHORS   Ingham,N.J., Pearson,S.A., Vancollie,V.E., Rook,V., Lewis,M.A.,
            Chen,J., Buniello,A., Martelletti,E., Preite,L., Lam,C.C.,
            Weiss,F.D., Powis,Z., Suwannarat,P., Lelliott,C.J., Dawson,S.J.,
            White,J.K. and Steel,K.P.
  TITLE     Mouse screen reveals multiple new genes underlying mouse and human
            hearing loss
  JOURNAL   PLoS Biol 17 (4), e3000194 (2019)
   PUBMED   30973865
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2971)
  AUTHORS   Larder,R., Sim,M.F.M., Gulati,P., Antrobus,R., Tung,Y.C.L.,
            Rimmington,D., Ayuso,E., Polex-Wolf,J., Lam,B.Y.H., Dias,C.,
            Logan,D.W., Virtue,S., Bosch,F., Yeo,G.S.H., Saudek,V.,
            O'Rahilly,S. and Coll,A.P.
  TITLE     Obesity-associated gene TMEM18 has a role in the central control of
            appetite and body weight regulation
  JOURNAL   Proc Natl Acad Sci U S A 114 (35), 9421-9426 (2017)
   PUBMED   28811369
REFERENCE   4  (bases 1 to 2971)
  AUTHORS   Piao,Y., Ko,N.T., Lim,M.K. and Ko,M.S.
  TITLE     Construction of long-transcript enriched cDNA libraries from
            submicrogram amounts of total RNAs by a universal PCR amplification
            method
  JOURNAL   Genome Res 11 (9), 1553-1558 (2001)
   PUBMED   11544199
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BY013255.1, AK160817.1 and AI835036.1.
            
            On Oct 6, 2007 this sequence version replaced NM_172049.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC024093.1, AK133493.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-9                 BY013255.1         2-10
            10-2954             AK160817.1         2-2946
            2955-2971           AI835036.1         1-17                c
FEATURES             Location/Qualifiers
     source          1..2971
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="12"
                     /map="12 13.0 cM"
     gene            1..2971
                     /gene="Tmem18"
                     /note="transmembrane protein 18"
                     /db_xref="GeneID:211986"
                     /db_xref="MGI:MGI:2387176"
     exon            1..120
                     /gene="Tmem18"
                     /inference="alignment:Splign:2.1.0"
     CDS             64..486
                     /gene="Tmem18"
                     /codon_start=1
                     /product="transmembrane protein 18"
                     /protein_id="NP_742046.2"
                     /db_xref="CCDS:CCDS49043.1"
                     /db_xref="GeneID:211986"
                     /db_xref="MGI:MGI:2387176"
                     /translation="MSSAYSVRSFPVSIPAVIMETDWTEPWLLGLLAFHLLCLLLTCF
                     SSQRYKLQIGHFLCLVVLVYSAEYINEVAAVNWRLFSKYQYFDSRGMFISLVFSAPLL
                     FNAMLIVIMWVRKTLTVMTDLKTLQEERKERRRRRKEE"
     exon            121..241
                     /gene="Tmem18"
                     /inference="alignment:Splign:2.1.0"
     exon            242..296
                     /gene="Tmem18"
                     /inference="alignment:Splign:2.1.0"
     exon            297..390
                     /gene="Tmem18"
                     /inference="alignment:Splign:2.1.0"
     exon            391..2958
                     /gene="Tmem18"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2938..2943
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Tmem18"
                     /note="hexamer: AATAAA"
     polyA_site      2958
                     /gene="Tmem18"
                     /note="major polyA site"
ORIGIN      
        1 taagcgcctt tcccggctgt cggaagctgc ggactcgtcc cggctgttgg gatccctggc
       61 gcgatgtcgt ccgcatactc agtccgatcc ttcccagtca gcatccctgc ggtgatcatg
      121 gagacagact ggacagagcc gtggctccta ggactccttg ccttccacct gctgtgcctc
      181 cttctcactt gcttctcctc acagaggtat aagctacaga tcgggcactt cctgtgtctg
      241 gtagtgttag tctatagtgc cgagtacatc aacgaagtgg ctgcagtgaa ctggagattg
      301 ttctcaaagt accagtactt tgactcccgg gggatgttca tttctcttgt gttctcagcg
      361 ccactgctct tcaacgccat gctcattgtg attatgtggg tacggaagac tttgactgtg
      421 atgaccgacc tgaagacctt acaagaggaa agaaaggaga ggaggaggag gaggaaggaa
      481 gagtaatggt gaactgctgc tgtgcgccat gtttgtcctc tgacttgctt gtttccagct
      541 gggaactaac tgcacatggc ccagatgcca aatgtcagcc gactcatggt ttagaaaaag
      601 gtccttatag cttagcacac cacaactggt tcttatagtt tactctgttt ggagccctct
      661 taaagctggt tcttaaaaga actgatggca tcaccgaaac acgttggatg gggacccctc
      721 ctttacatgg ggagagcttc ctggttcagc cactttattc acagcttctc acacgttgct
      781 ggtagacctc attatgtaac tagagttcac gaggttctaa gtgtggacac tgggaaataa
      841 agagccccga tcctgctggg atcactacct gctccctctt ctgtttctag cctgtagcgg
      901 tggctatcag ctaagggtgg ctttgttccc caggaacttt gctgatgctt gtcaccgcag
      961 gtggacatgg tacttgtgtc cataggacag agccgagtcc tgcagggcac aggacagccc
     1021 caccgtggag tattgctgtg tacataatgt cagcagtgcc caggctgagg gagcggtggg
     1081 tcaggtctgg ggaagaatgt ggtgtgtttc agttcccatg aagctaggcc tctaaataat
     1141 tgagttgggg ctatgccact gggaatgttt tgtttaaaaa tattaagcta gaatgaaagc
     1201 tgtaaagtca agactccgtg gaaaatagta tgtcaaatga accgtttggt cttgtttttt
     1261 ctggatacag aattataaat acctgcatgc tctgtgaatg gcttatctgt ttctacccag
     1321 actgtgacaa gtgcagacag ttcttcccaa gtgtcacatt tgtgcggttg ggggctggcg
     1381 aacacagcag ggtgtattgt cacctgtgcc tctgttttgt cagacaccct tggtttccat
     1441 ggaatccgga gcaataaacc tttagagatt tgaggtgaca gagacccaga cttagagaca
     1501 gtaagaaata cactcttggc tttagtttaa agaaataatt tgtgaagtat tttctttatt
     1561 taataggatt ttttcctttc taaacattct tcaaagttct aagctaaaat aaaatctagt
     1621 taaaaatatt taaaaggtta aaagtagatc gtttgataga ctaccgttat gttctgaaac
     1681 ccagttatat agtttctaat gtttatccag cttccggatt taacagaaac ctactcactg
     1741 tcttgtgaca atctgatcat cctgtggtgt gagtttgcgg ctcctgctgc agcacttaac
     1801 ctgcattgtg cacagtctct tgatttgttc cacttcacat tgaacaaaaa agtgccaagg
     1861 aaaataacac agtgttaagt tcagattttg aatttaaatc acactgttaa gtcgtggaca
     1921 ttgtgtctaa agggatactt aattgtatgt ttctgaagtt ttctggttca ggatatttga
     1981 tcattttatg gttacaaagg tgcttactat aacattgatt agtttcatag aaaataagaa
     2041 caaattagtc cgttagataa atctgaaaca actgagtgtc agataggcct ggactcacca
     2101 actagatgtg ttcccttcag gcctttgtgt ctgaggccag tggggcagaa tggggcagag
     2161 agcggctgcg atgtccgcgt tctggcatat gccggtatta atacctcagc ttgtgtaacc
     2221 atgaccctga ccggctttct tgccttgttt ctgcacgtgt ggttgtggat gcacatgtgc
     2281 ttcgtttttt ccataattaa atgtttatgt tttgttttgt atttttaaac gcaagttttg
     2341 gattagagag atggcttcgt tggcactctc cacctgagtt tggtctccag aggaacttaa
     2401 catttgttcc caagcattac acgctaagtc ccattgctgg gaaaacctgc ctgatggaag
     2461 gacggaagcg gccactacag gttgtcctct aactgtatgt gcacacaaaa taaagtgatg
     2521 cacgagtcac tcccgaccaa cagcacttga ggtggccctc tggctttcac acacacatgt
     2581 gtgcacactg ctacacatat agcatctaca caaatgttgt gtgttttctg acattcactc
     2641 tgtgactaaa attatacttg gaggaacttt taaacatttt gccctgatat taaggaaaat
     2701 cctgcaaggt gcaactatga acaagcagtt gggttctgtg ctgctttggg gattttacgg
     2761 gtttgcccag tgctgtgttc acacttccgt aaaggaaggt ttacaggcac tgatggacac
     2821 agtgagcact gagtttactg agtagttgtg gaaagctctg gaaagcccta aaataaccat
     2881 cttatacatt ccagtctatg aaacttcatg tttgttgatt attatgcatt aatatacaat
     2941 aaactatgtt tgataataaa aaaaaaaaaa a
//
