LOCUS       NM_025326               1064 bp    mRNA    linear   ROD 02-JUN-2024
DEFINITION  Mus musculus transmembrane protein 176A (Tmem176a), transcript
            variant 1, mRNA.
ACCESSION   NM_025326 XM_981912
VERSION     NM_025326.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1064)
  AUTHORS   Lancien,M., Bienvenu,G., Salle,S., Gueno,L., Feyeux,M., Merieau,E.,
            Remy,S., Even,A., Moreau,A., Molle,A., Fourgeux,C., Coulon,F.,
            Beriou,G., Bouchet-Delbos,L., Chiffoleau,E., Kirstetter,P.,
            Chan,S., Kerfoot,S.M., Abdu Rahiman,S., De Simone,V., Matteoli,G.,
            Boncompain,G., Perez,F., Josien,R., Poschmann,J., Cuturi,M.C. and
            Louvet,C.
  TITLE     Dendritic Cells Require TMEM176A/B Ion Channels for Optimal MHC
            Class II Antigen Presentation to Naive CD4+ T Cells
  JOURNAL   J Immunol 207 (2), 421-435 (2021)
   PUBMED   34233909
  REMARK    GeneRIF: Dendritic Cells Require TMEM176A/B Ion Channels for
            Optimal MHC Class II Antigen Presentation to Naive CD4(+) T Cells.
REFERENCE   2  (bases 1 to 1064)
  AUTHORS   Lemoine,A., Chauveau-Le Friec,G., Langa,F. and Louvet,C.
  TITLE     Generation of a Double KO Mouse by Simultaneous Targeting of the
            Neighboring Genes Tmem176a and Tmem176b Using CRISPR/Cas9: Key
            Steps from Design to Genotyping
  JOURNAL   J Genet Genomics 43 (5), 329-340 (2016)
   PUBMED   27234594
REFERENCE   3  (bases 1 to 1064)
  AUTHORS   Drujont,L., Lemoine,A., Moreau,A., Bienvenu,G., Lancien,M.,
            Cens,T., Guillot,F., Beriou,G., Bouchet-Delbos,L., Fehling,H.J.,
            Chiffoleau,E., Nicot,A.B., Charnet,P., Martin,J.C., Josien,R.,
            Cuturi,M.C. and Louvet,C.
  TITLE     RORgammat+ cells selectively express redundant cation channels
            linked to the Golgi apparatus
  JOURNAL   Sci Rep 6, 23682 (2016)
   PUBMED   27009467
  REMARK    GeneRIF: Study characterized Tmem176a and b expression in RORgamma
            t+ lymphocytes at transcriptional and protein levels and present
            evidence that both genes exert a redundant ion channel function
            related to a colocalisation in close proximity to the Golgi
            apparatus.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 1064)
  AUTHORS   Lumayag,S., Haldin,C.E., Corbett,N.J., Wahlin,K.J., Cowan,C.,
            Turturro,S., Larsen,P.E., Kovacs,B., Witmer,P.D., Valle,D.,
            Zack,D.J., Nicholson,D.A. and Xu,S.
  TITLE     Inactivation of the microRNA-183/96/182 cluster results in
            syndromic retinal degeneration
  JOURNAL   Proc Natl Acad Sci U S A 110 (6), E507-E516 (2013)
   PUBMED   23341629
REFERENCE   5  (bases 1 to 1064)
  AUTHORS   Tamplin,O.J., Cox,B.J. and Rossant,J.
  TITLE     Integrated microarray and ChIP analysis identifies multiple Foxa2
            dependent target genes in the notochord
  JOURNAL   Dev Biol 360 (2), 415-425 (2011)
   PUBMED   22008794
REFERENCE   6  (bases 1 to 1064)
  AUTHORS   Condamine,T., Le Texier,L., Howie,D., Lavault,A., Hill,M.,
            Halary,F., Cobbold,S., Waldmann,H., Cuturi,M.C. and Chiffoleau,E.
  TITLE     Tmem176B and Tmem176A are associated with the immature state of
            dendritic cells
  JOURNAL   J Leukoc Biol 88 (3), 507-515 (2010)
   PUBMED   20501748
  REMARK    GeneRIF: These data suggest that Tmem176B and Tmem176A associate to
            form multimers and restrain dendritic cell maturation.
REFERENCE   7  (bases 1 to 1064)
  AUTHORS   Quina,L.A., Wang,S., Ng,L. and Turner,E.E.
  TITLE     Brn3a and Nurr1 mediate a gene regulatory pathway for habenula
            development
  JOURNAL   J Neurosci 29 (45), 14309-14322 (2009)
   PUBMED   19906978
REFERENCE   8  (bases 1 to 1064)
  AUTHORS   Garcia-Frigola,C., Burgaya,F., Calbet,M., Lopez-Domenech,G., de
            Lecea,L. and Soriano,E.
  TITLE     A collection of cDNAs enriched in upper cortical layers of the
            embryonic mouse brain
  JOURNAL   Brain Res Mol Brain Res 122 (2), 133-150 (2004)
   PUBMED   15010206
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BY018646.1 and AK002546.1.
            
            On May 25, 2007 this sequence version replaced NM_025326.3.
            
            Transcript Variant: This variant (1) represents the longer
            transcript. Both variants 1 and 2 encode the same protein.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK002546.1, AK004315.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-241               BY018646.1         18-258
            242-1064            AK002546.1         218-1040
FEATURES             Location/Qualifiers
     source          1..1064
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="6"
                     /map="6 23.75 cM"
     gene            1..1064
                     /gene="Tmem176a"
                     /gene_synonym="0610011I04Rik; Keg2"
                     /note="transmembrane protein 176A"
                     /db_xref="GeneID:66058"
                     /db_xref="MGI:MGI:1913308"
     exon            1..107
                     /gene="Tmem176a"
                     /gene_synonym="0610011I04Rik; Keg2"
                     /inference="alignment:Splign:2.1.0"
     exon            108..305
                     /gene="Tmem176a"
                     /gene_synonym="0610011I04Rik; Keg2"
                     /inference="alignment:Splign:2.1.0"
     CDS             111..845
                     /gene="Tmem176a"
                     /gene_synonym="0610011I04Rik; Keg2"
                     /note="kidney expressed gene 2; gene signature 188;
                     kidney-expressed gene 2 protein"
                     /codon_start=1
                     /product="transmembrane protein 176A"
                     /protein_id="NP_079602.4"
                     /db_xref="CCDS:CCDS39482.1"
                     /db_xref="GeneID:66058"
                     /db_xref="MGI:MGI:1913308"
                     /translation="MSTDMETAVVGKVDPEAPQPTHIDVHIHQESALAKLLLAGCSLL
                     RIPASASTQSQGSSRVLVASWVVQTVLGALSVVLGGTLYIGHYLAMYSEGAPFWTGIV
                     AMLAGAVAFLHKKRGGTCWALMRTLLVLASFCTAVAAIVIGSRELNFYWYFLGDDVCQ
                     RDSSYGWSTMPRTTPVPEEADRIALCIYYTSMLKTLLMSLQAMLLGIWVLLLLASLTP
                     VCVYIWKRFFTKAETEEKKLLGAAVI"
     exon            306..416
                     /gene="Tmem176a"
                     /gene_synonym="0610011I04Rik; Keg2"
                     /inference="alignment:Splign:2.1.0"
     exon            417..473
                     /gene="Tmem176a"
                     /gene_synonym="0610011I04Rik; Keg2"
                     /inference="alignment:Splign:2.1.0"
     exon            474..692
                     /gene="Tmem176a"
                     /gene_synonym="0610011I04Rik; Keg2"
                     /inference="alignment:Splign:2.1.0"
     exon            693..803
                     /gene="Tmem176a"
                     /gene_synonym="0610011I04Rik; Keg2"
                     /inference="alignment:Splign:2.1.0"
     exon            804..1064
                     /gene="Tmem176a"
                     /gene_synonym="0610011I04Rik; Keg2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1040..1045
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Tmem176a"
                     /gene_synonym="0610011I04Rik; Keg2"
                     /note="putative"
     polyA_site      1064
                     /gene="Tmem176a"
                     /gene_synonym="0610011I04Rik; Keg2"
                     /note="putative"
ORIGIN      
        1 cccaggttct tcctcctgcc agggctgaag ttctcaacta ctgccagcgc ctctgtgtga
       61 gctctggcct tccctgctgc acacttcccc ggtcccagag gaatcagacg atgtccacag
      121 acatggagac tgcagtcgtc ggcaaggtgg accctgaggc tccacaaccc acccacattg
      181 atgtgcacat ccaccaggag tctgctctgg ccaaacttct gctggccgga tgctcattgc
      241 taaggattcc agcatccgct tccacccaga gccagggcag cagcagagtg ctggtggcct
      301 cctgggtggt gcagactgtg ctgggggctc tgagtgtggt tctgggtgga accctctaca
      361 taggccatta tttagccatg tattccgaag gcgccccctt ctggactggg atcgtggcta
      421 tgctggctgg agctgttgcc ttccttcaca agaaacgggg tggtacctgc tgggccctga
      481 tgaggaccct tcttgtgctg gcaagtttct gcaccgctgt ggctgccatc gttattgggt
      541 ctcgtgagtt gaatttttac tggtattttc tcggagatga tgtctgtcaa agagactctt
      601 catatggatg gtccaccatg cctagaacca ctccagttcc cgaagaagct gataggattg
      661 ccttgtgcat atactacaca agcatgctaa agaccctgct catgagcctc caagctatgc
      721 tcttgggtat ctgggtgctg ctgctcctgg cttctctcac ccctgtatgt gtctacatct
      781 ggaaaagatt tttcacaaag gcggaaacag aggagaagaa actgctgggt gcagctgtga
      841 tctagccttt cctcttgctc cgggcgtccc tcctactgaa gcctgaaaga agaatcaggc
      901 aggactaaga agaccctccc ccactagcag ggccatggcc actgcctggt tctgcccagc
      961 accacagcag ctctcagcag cacttgcttg tctctccatc cttcaccgtc ctatatccct
     1021 cctcaggcag caacttgata ataaactctc ctgttattgc tggc
//
