LOCUS       NM_001163532            2966 bp    mRNA    linear   ROD 09-FEB-2025
DEFINITION  Mus musculus transmembrane protein 175 (Tmem175), transcript
            variant 3, mRNA.
ACCESSION   NM_001163532
VERSION     NM_001163532.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2966)
  AUTHORS   Feng,T., Zheng,H., Zhang,Z., Fan,P. and Yang,X.
  TITLE     Mechanism and therapeutic targets of the involvement of a novel
            lysosomal proton channel TMEM175 in Parkinson's disease
  JOURNAL   Ageing Res Rev 100, 102373 (2024)
   PUBMED   38960046
  REMARK    GeneRIF: Mechanism and therapeutic targets of the involvement of a
            novel lysosomal proton channel TMEM175 in Parkinson's disease.
            Review article
REFERENCE   2  (bases 1 to 2966)
  AUTHORS   Su,J., Wang,Y., Yao,J., Sun,L., Zhao,C., Liu,L. and Zhang,L.
  TITLE     Systemic knockout of Tmem175 results in aberrant differentiation
            but no effect on hematopoietic reconstitution
  JOURNAL   Stem Cell Res 79, 103469 (2024)
   PUBMED   38878670
  REMARK    GeneRIF: Systemic knockout of Tmem175 results in aberrant
            differentiation but no effect on hematopoietic reconstitution.
REFERENCE   3  (bases 1 to 2966)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   4  (bases 1 to 2966)
  AUTHORS   Qu,L., Lin,B., Zeng,W., Fan,C., Wu,H., Ge,Y., Li,Q., Li,C., Wei,Y.,
            Xin,J., Wang,X., Liu,D. and Cang,C.
  TITLE     Lysosomal K+ channel TMEM175 promotes apoptosis and aggravates
            symptoms of Parkinson's disease
  JOURNAL   EMBO Rep 23 (9), e53234 (2022)
   PUBMED   35913019
  REMARK    GeneRIF: Lysosomal K(+) channel TMEM175 promotes apoptosis and
            aggravates symptoms of Parkinson's disease.
REFERENCE   5  (bases 1 to 2966)
  AUTHORS   Hu,M., Li,P., Wang,C., Feng,X., Geng,Q., Chen,W., Marthi,M.,
            Zhang,W., Gao,C., Reid,W., Swanson,J., Du,W., Hume,R.I. and Xu,H.
  TITLE     Parkinson's disease-risk protein TMEM175 is a proton-activated
            proton channel in lysosomes
  JOURNAL   Cell 185 (13), 2292-2308 (2022)
   PUBMED   35750034
REFERENCE   6  (bases 1 to 2966)
  AUTHORS   Pergel,E., Veres,I., Csigi,G.I. and Czirjak,G.
  TITLE     Translocation of TMEM175 Lysosomal Potassium Channel to the Plasma
            Membrane by Dynasore Compounds
  JOURNAL   Int J Mol Sci 22 (19), 10515 (2021)
   PUBMED   34638858
  REMARK    GeneRIF: Translocation of TMEM175 Lysosomal Potassium Channel to
            the Plasma Membrane by Dynasore Compounds.
            Publication Status: Online-Only
REFERENCE   7  (bases 1 to 2966)
  AUTHORS   Wie,J., Liu,Z., Song,H., Tropea,T.F., Yang,L., Wang,H., Liang,Y.,
            Cang,C., Aranda,K., Lohmann,J., Yang,J., Lu,B., Chen-Plotkin,A.S.,
            Luk,K.C. and Ren,D.
  TITLE     A growth-factor-activated lysosomal K+ channel regulates
            Parkinson's pathology
  JOURNAL   Nature 591 (7850), 431-437 (2021)
   PUBMED   33505021
  REMARK    Erratum:[Nature. 2021 Apr;592(7855):E10. doi:
            10.1038/s41586-021-03438-x.. PMID: 33790472]
REFERENCE   8  (bases 1 to 2966)
  AUTHORS   Cang,C., Aranda,K., Seo,Y.J., Gasnier,B. and Ren,D.
  TITLE     TMEM175 Is an Organelle K(+) Channel Regulating Lysosomal Function
  JOURNAL   Cell 162 (5), 1101-1112 (2015)
   PUBMED   26317472
  REMARK    GeneRIF: TMEM175 comprises a potassium (K) channel that underlies
            the molecular mechanism of lysosomal K(+) permeability.
REFERENCE   9  (bases 1 to 2966)
  AUTHORS   Chen,H., Palmer,J.S., Thiagarajan,R.D., Dinger,M.E., Lesieur,E.,
            Chiu,H., Schulz,A., Spiller,C., Grimmond,S.M., Little,M.H.,
            Koopman,P. and Wilhelm,D.
  TITLE     Identification of novel markers of mouse fetal ovary development
  JOURNAL   PLoS One 7 (7), e41683 (2012)
   PUBMED   22844512
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BB653267.1, AI526594.1, AK013879.1 and AK034579.1.
            
            Transcript Variant: This variant (3) differs in the 5' UTR compared
            to variant 4. Variants 1, 3, and 4 all encode the same isoform (1).
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK013879.1, AK170273.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-29                BB653267.1         38-66
            30-653              AI526594.1         1-624
            654-1623            AK013879.1         584-1553
            1624-2966           AK034579.1         1600-2942
FEATURES             Location/Qualifiers
     source          1..2966
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="5"
                     /map="5 53.24 cM"
     gene            1..2966
                     /gene="Tmem175"
                     /gene_synonym="3010001K23Rik; mTMEM175"
                     /note="transmembrane protein 175"
                     /db_xref="GeneID:72392"
                     /db_xref="MGI:MGI:1919642"
     exon            1..91
                     /gene="Tmem175"
                     /gene_synonym="3010001K23Rik; mTMEM175"
                     /inference="alignment:Splign:2.1.0"
     exon            92..345
                     /gene="Tmem175"
                     /gene_synonym="3010001K23Rik; mTMEM175"
                     /inference="alignment:Splign:2.1.0"
     CDS             202..1701
                     /gene="Tmem175"
                     /gene_synonym="3010001K23Rik; mTMEM175"
                     /note="isoform 1 is encoded by transcript variant 3;
                     endosomal/lysomomal potassium channel TMEM175;
                     endosomal/lysosomal potassium channel TMEM175;
                     endosomal/lysosomal proton channel TMEM175"
                     /codon_start=1
                     /product="endosomal/lysosomal proton channel TMEM175
                     isoform 1"
                     /protein_id="NP_001157004.1"
                     /db_xref="CCDS:CCDS19514.1"
                     /db_xref="GeneID:72392"
                     /db_xref="MGI:MGI:1919642"
                     /translation="MSRLQTEEQAVDSEGDSSLHRRNEEGTQSSHRMLGFSDALLSII
                     ATVMILPVTHTEISPEQQFDKSIQKLLATRIAVYLMTFLIVTVAWTAHTRLFQVVGKI
                     DDTLALLNLACMMTITLLPYTFSLMVTFPDVPLGIFLFCVCVIAIGSVQAMIVGYAFH
                     FPHLLNPQIQCSTHRDLSRRHILHLVLRGPALCFVAAVFSLFFFPLSYLLMVTVIFLP
                     HISKATTWCKDKLMGQRESPAHDMEPFSIDLHAPLSKERVEAFSDGVYAIVATLLILD
                     ICEDNVPDPKDVQEKFSGSLVAALGAYGPQFLAYFGSFATVGLLWFAHHSLFLHVRKA
                     TQTMGLLNILSLAFVGGLPLAYQQTSAFARQPHDELERVRVSCAIIFFASIFQFAIWT
                     TALLHQTETLQPAVQFGGQEHAFMFAKLALYPCASLLAFAATCLLSRFSTAIFHLMQI
                     SVPFAFLLLRLLVRLALAGLQVLRGLWPHHPQQDQSEPEAQSQLLPDPC"
     exon            346..384
                     /gene="Tmem175"
                     /gene_synonym="3010001K23Rik; mTMEM175"
                     /inference="alignment:Splign:2.1.0"
     exon            385..482
                     /gene="Tmem175"
                     /gene_synonym="3010001K23Rik; mTMEM175"
                     /inference="alignment:Splign:2.1.0"
     exon            483..534
                     /gene="Tmem175"
                     /gene_synonym="3010001K23Rik; mTMEM175"
                     /inference="alignment:Splign:2.1.0"
     exon            535..570
                     /gene="Tmem175"
                     /gene_synonym="3010001K23Rik; mTMEM175"
                     /inference="alignment:Splign:2.1.0"
     exon            571..654
                     /gene="Tmem175"
                     /gene_synonym="3010001K23Rik; mTMEM175"
                     /inference="alignment:Splign:2.1.0"
     exon            655..819
                     /gene="Tmem175"
                     /gene_synonym="3010001K23Rik; mTMEM175"
                     /inference="alignment:Splign:2.1.0"
     exon            820..898
                     /gene="Tmem175"
                     /gene_synonym="3010001K23Rik; mTMEM175"
                     /inference="alignment:Splign:2.1.0"
     exon            899..1034
                     /gene="Tmem175"
                     /gene_synonym="3010001K23Rik; mTMEM175"
                     /inference="alignment:Splign:2.1.0"
     exon            1035..2966
                     /gene="Tmem175"
                     /gene_synonym="3010001K23Rik; mTMEM175"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2436..2441
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Tmem175"
                     /gene_synonym="3010001K23Rik; mTMEM175"
                     /note="hexamer: AATGAA"
     polyA_site      2456
                     /gene="Tmem175"
                     /gene_synonym="3010001K23Rik; mTMEM175"
                     /note="major polyA site"
ORIGIN      
        1 gggcgcgtgt ttctgcagag ccagcagcag cagcaggcgc aggcgcagag ggtgcgatcc
       61 tacggacctc aagctcctag gaggcacgga gcacttgtct ctgcatcgaa atgcctaacg
      121 tctacagggt tgagacatcc ctctgatatg ctcagagcta cacttcttcc ccggctgccg
      181 tgacaacttg ttggtcccac catgtccagg ctccagactg aggagcaggc agtggattcc
      241 gagggagact catccctaca caggagaaat gaggagggga cacagagctc ccaccgcatg
      301 ctgggcttca gtgatgcact gctgtccatc atcgccactg tcatgatcct gcctgtgacc
      361 cacacagaga tctcaccaga acagcagttt gacaaaagta tacagaaact cctagcaact
      421 aggatcgctg tctacctcat gacatttcta atcgtaactg tggcctggac agcacatacc
      481 agattgttcc aggttgttgg aaaaatagat gatacacttg ccttgctcaa cctggcctgt
      541 atgatgacta tcaccctcct gccctacaca ttttcactaa tggtaacatt ccctgatgtg
      601 cccctgggca tcttcctgtt ctgcgtgtgt gtgatcgcca ttggatctgt gcaggcaatg
      661 attgtgggct atgccttcca cttcccacat ctgctgaatc cacaaatcca gtgctctaca
      721 catagggatc tatcccggcg gcatatcctg cacctggtcc tccgtggccc agcactgtgt
      781 tttgttgcgg ctgtcttttc cctcttcttc ttccccttgt catacctgct gatggtgact
      841 gtcatcttcc tccctcacat cagcaaggct accacctggt gcaaagacaa gttgatgggc
      901 caaagggagt ctccagctca cgacatggag cccttcagta ttgacctgca tgcgcccctc
      961 agcaaagagc gggtggaagc tttcagcgac ggtgtctatg ccatcgtggc cacactcctc
     1021 atcctggaca tctgtgagga caatgttccg gatcccaagg atgtgcaaga gaaattcagt
     1081 ggcagccttg tggctgccct gggtgcatat gggccacaat tcttggcata cttcggctcc
     1141 ttcgccacag tgggtctcct ctggtttgct caccattcac tcttcctgca tgtccggaag
     1201 gctacacaga ccatggggtt actcaacata ctatcgctgg cctttgtggg tggccttcct
     1261 ctggcctacc agcagacctc agcttttgcc cggcaacccc atgatgaact tgagcgtgtg
     1321 cgtgtcagct gtgccatcat cttctttgcc agcatcttcc agtttgccat ctggactaca
     1381 gccctgctgc atcagacaga aacactgcag ccagctgtgc aatttggtgg gcaggagcat
     1441 gctttcatgt ttgccaagct tgcactgtat ccctgtgcca gcctgctggc ctttgctgcc
     1501 acctgcctac tgagtcgttt cagcacagcc atcttccacc tcatgcagat ttcagtgccc
     1561 tttgcattcc tgttgctccg tctccttgta cgccttgccc tggcaggcct tcaagtccta
     1621 cggggcctct ggccacacca tccccaacaa gaccagagtg aacctgaggc acagtcccag
     1681 ctcttgcctg acccctgcta atgccaagaa agccactcct gcctatccta ggcatattgt
     1741 aaggggtcct atggagatgt cttagtacaa gtttattgtg acactggggc tgtggatata
     1801 ttggcctggg tattgtttac attgtgaaat atcaagatct atttcaagag tatgaacatt
     1861 gttcacactt tgcagtgaag ggtttcatcg gaactgaatt tattgttttt acccatttac
     1921 tttcctccct tgcccacaac attgccaggg gaatttatct gtctgattaa cctgaacaag
     1981 ccactttgag cttgagatct tgtttgaggc agcagcatcc agccaggatc aagggtctat
     2041 cctatgcctt gctctgacct gcatgtgacc atacaggcga tcctccttct tggaacgttc
     2101 tagcaatgga gtctaggcta actcagcctg agaagctcct gtctggctca tatcctcaaa
     2161 agtacagagc ttccctggcg ggactgccag tctgtttgcc cattcctgta ccagttgtta
     2221 gacaaagaca agagtcagct atgggacagc aaaacccaca ctgtgtattt ggcccagata
     2281 taacttagat cacattcagg tagtgataaa ttacacacaa agacaggacc agtcagtgca
     2341 caggacacac tccacataaa gacagtctca cttaaataca gaggacatgc tacactgacc
     2401 gtaatttaca aacaattcct acagattgaa ataaaaatga aaaattacat cacaaagaat
     2461 ttcttttgaa cattgaaaat gatgtaaaca tgatcatcgg cactgagctc acacatggct
     2521 atctgatgca cttgtatgca gttttggcag aagcaggggg tggggtaggg gggggtggca
     2581 caatggtccc atgaagctgt caggcaggga gacaccctgg tccacacact accctggtat
     2641 cacacttctg aaggtgtcca caagccaagt aatgacacac aggagaaggc tctgcactga
     2701 ctggcttttg ggggtggggg gtactcatca caggagactt cccacatagt cttccccgag
     2761 ccaggcctgg ggctagacct ttagatgtct ttagcatttt tttttaatgt tttcactttt
     2821 tgagaggggc tttagcgcta taacccagtt atgctttagc ctggagaaat gaattatagg
     2881 catgtaccag gcttagctag gcagctcatt tttggacaaa ttctttctag ggtcttaagt
     2941 gtgacaatga attcttgggt gcatgg
//
