LOCUS       NM_028990               4583 bp    mRNA    linear   ROD 09-FEB-2025
DEFINITION  Mus musculus transmembrane protein 168 (Tmem168), transcript
            variant 1, mRNA.
ACCESSION   NM_028990
VERSION     NM_028990.5
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4583)
  AUTHORS   Nguyen,L.K.C., Shimizu,A., Soh,J.E.C., Komeno,M., Sato,A. and
            Ogita,H.
  TITLE     Transmembrane protein 168 mutation reduces cardiomyocyte cell
            surface expression of Nav1.5 through alphaB-crystallin
            intracellular dynamics
  JOURNAL   J Biochem 170 (5), 577-585 (2021)
   PUBMED   34086898
  REMARK    GeneRIF: Transmembrane protein 168 mutation reduces cardiomyocyte
            cell surface expression of Nav1.5 through alphaB-crystallin
            intracellular dynamics.
            Erratum:[J Biochem. 2022 Mar 31;171(4):467. doi:
            10.1093/jb/mvab152.. PMID: 35137094]
REFERENCE   2  (bases 1 to 4583)
  AUTHORS   Shimizu,A., Zankov,D.P., Sato,A., Komeno,M., Toyoda,F.,
            Yamazaki,S., Makita,T., Noda,T., Ikawa,M., Asano,Y., Miyashita,Y.,
            Takashima,S., Morita,H., Ishikawa,T., Makita,N., Hitosugi,M.,
            Matsuura,H., Ohno,S., Horie,M. and Ogita,H.
  TITLE     Identification of transmembrane protein 168 mutation in familial
            Brugada syndrome
  JOURNAL   FASEB J 34 (5), 6399-6417 (2020)
   PUBMED   32175648
REFERENCE   3  (bases 1 to 4583)
  AUTHORS   Sansom,S.N., Griffiths,D.S., Faedo,A., Kleinjan,D.J., Ruan,Y.,
            Smith,J., van Heyningen,V., Rubenstein,J.L. and Livesey,F.J.
  TITLE     The level of the transcription factor Pax6 is essential for
            controlling the balance between neural stem cell self-renewal and
            neurogenesis
  JOURNAL   PLoS Genet 5 (6), e1000511 (2009)
   PUBMED   19521500
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC139884.8 and AK051532.1.
            
            On Nov 15, 2016 this sequence version replaced NM_028990.4.
            
            Transcript Variant: This variant (1) represents the longer,
            protein-coding transcript.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK051532.1, BC007160.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-99                AC139884.8         48704-48802
            100-3899            AK051532.1         66-3865
            3900-4583           AC139884.8         75430-76113
FEATURES             Location/Qualifiers
     source          1..4583
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="6"
                     /map="6 5.68 cM"
     gene            1..4583
                     /gene="Tmem168"
                     /gene_synonym="5730526F17Rik; 8430437G11Rik"
                     /note="transmembrane protein 168"
                     /db_xref="GeneID:101118"
                     /db_xref="MGI:MGI:1921794"
     exon            1..269
                     /gene="Tmem168"
                     /gene_synonym="5730526F17Rik; 8430437G11Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            270..1519
                     /gene="Tmem168"
                     /gene_synonym="5730526F17Rik; 8430437G11Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             392..2485
                     /gene="Tmem168"
                     /gene_synonym="5730526F17Rik; 8430437G11Rik"
                     /codon_start=1
                     /product="transmembrane protein 168"
                     /protein_id="NP_083266.1"
                     /db_xref="CCDS:CCDS19915.1"
                     /db_xref="GeneID:101118"
                     /db_xref="MGI:MGI:1921794"
                     /translation="MCRSLRYCVSHCLYLAMTRLEEVNREVNMHSSVRYLGYLARINL
                     LVAICLGLYVRWEKTANSLILVIFILGLFVLGIASILYYYFSMEAASLSLSNLWFGFL
                     LGLLCFLDNSSFKSDVKEETTKYLLLTSIVLRILCALVERISGYVRHRPTLLTTVEFL
                     ELVGFAIASTTMLVEKSLSVILLVMALAMLIIDLRMKSFLAIPNLIIFSVLLFFSSLE
                     TPQNPIAFACFFICLVTDPFLDIYFSGLSVTERWKPFLHRGRICRRLSVLFTAMIELT
                     FFILSAFKLRDTHLWYFVIPGFSIFGFFWMICHIIFLLTLWGFHTKLNDCHKVYINHR
                     ADNNSLDRIMASKGMRHFCLISEQLVFFSLLATAILGAVSWQPTNGIFLSMFLIVLPL
                     ESMAHGLFHELGNCLGGTSVGYAIVIPTNFCSPDGQPTLLPPEHVQELNLRSTGMLNA
                     IQRFFAYHMIETYGCDYSTSGLSFDTLHSKLKAFLELRTVDGPRHDTYVLYYSGHTHG
                     SGEWALAGGDILRLDTLLEWWREKNGSFCSRLIIILDSENSTPWVKEVRKINDQYVAV
                     QGAELAKTVDIEEADPPQLGDFTRDWVEYNCNSTNNICWTEKGRTVRAVYGVSKRWSD
                     YTLHLPTGSDVAKHWMLHFPRVTYPLVHLANWLCGLNLFWVCKACFRCLKRLKMSWFL
                     PTVLDTGQGFKLVKS"
     exon            1520..1662
                     /gene="Tmem168"
                     /gene_synonym="5730526F17Rik; 8430437G11Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1663..1937
                     /gene="Tmem168"
                     /gene_synonym="5730526F17Rik; 8430437G11Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1938..4583
                     /gene="Tmem168"
                     /gene_synonym="5730526F17Rik; 8430437G11Rik"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3955..3960
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Tmem168"
                     /gene_synonym="5730526F17Rik; 8430437G11Rik"
                     /note="hexamer: ATTAAA"
     polyA_site      3978
                     /gene="Tmem168"
                     /gene_synonym="5730526F17Rik; 8430437G11Rik"
                     /note="major polyA site"
     regulatory      4140..4145
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Tmem168"
                     /gene_synonym="5730526F17Rik; 8430437G11Rik"
                     /note="hexamer: AGTAAA"
     regulatory      4154..4159
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Tmem168"
                     /gene_synonym="5730526F17Rik; 8430437G11Rik"
                     /note="hexamer: AATAAA"
     polyA_site      4162
                     /gene="Tmem168"
                     /gene_synonym="5730526F17Rik; 8430437G11Rik"
     polyA_site      4176
                     /gene="Tmem168"
                     /gene_synonym="5730526F17Rik; 8430437G11Rik"
ORIGIN      
        1 gagcggctag gaggccgggc cagggaagga agcgcgcggc gcgccttgtc cctgacggcg
       61 atccgtagcg ctgggagtca gcggcggaaa catggcggcc gtgtcggtcc tagcgaaagt
      121 ttctaccgag gccttgcggt gctagtgcgg gaggcctcgg gctcacgtcc ccacatcacc
      181 tccgcgttct ggctcggagg ctccgcgccc tttggctctg cgagcttcgc agcggaaaga
      241 gcgaggctcg ctggcgaccg ctgactcggg tcattacaaa gaaaacttct gagagaatgt
      301 tggagtaggc attaccaaca ccttggcttt attgaatgaa atacaggaag ttaatttttg
      361 tgcctcttgg gaccctccag tgccagatta catgtgcaga tcactgcgat actgtgttag
      421 tcattgtctc tatctagcaa tgactaggct ggaagaagta aatagagaag tgaacatgca
      481 ttcctccgtg cggtacctcg gctatctagc caggatcaac ttacttgtgg ccatatgttt
      541 aggcctttat gtaagatggg aaaaaacagc aaattcctta attttggtga tttttatcct
      601 cggcctcttt gttcttggga tcgccagcat actctactat tacttttcaa tggaagcagc
      661 cagtttaagt ctctccaatc tctggtttgg atttctgctt ggccttctgt gttttcttga
      721 taattcatcc tttaaaagtg atgtgaaaga agaaacaacc aaatatttgc ttctgacatc
      781 tatagtttta aggatattgt gtgctttggt ggagagaatt tctggatatg tccgccatcg
      841 gcctacttta ctaaccacag tggagtttct ggaacttgtt gggtttgcca tcgccagcac
      901 aactatgttg gtggagaagt ctctgagtgt tattttgctg gttatggctt tggccatgct
      961 cattatcgac ctgagaatga agtcttttct agctattcca aacttaatca ttttttcagt
     1021 attgttattt ttctcttcat tggaaactcc ccaaaatcca attgcttttg catgtttttt
     1081 tatttgcctg gtaactgatc cttttcttga catttatttt agtggacttt cagtaactga
     1141 gaggtggaaa ccttttctgc atcgtgggag aatttgcagg agactttctg tgcttttcac
     1201 tgcaatgatt gagcttacat tcttcatcct ttcagcattt aaacttagag acactcatct
     1261 ctggtatttt gtgataccag gtttttccat tttcggattt ttctggatga tttgccatat
     1321 tatttttctt ttaactcttt ggggatttca tactaagttg aatgactgcc ataaagtgta
     1381 tatcaaccac agggcagata acaatagcct tgacagaatc atggcatcca aagggatgcg
     1441 tcatttttgc ttaatttcag agcagttagt tttttttagt cttcttgcaa cggcaatttt
     1501 gggagcagtc tcctggcagc caaccaatgg aatcttcttg agcatgtttc tcattgtctt
     1561 gccgctggag tccatggctc atgggctctt ccatgaactt ggcaactgct tagggggaac
     1621 ctctgttgga tacgctattg tgatccctac caacttctgc agccctgatg gccagccaac
     1681 actgcttccg ccagaacatg tacaggagtt aaatttgagg tctactggca tgctcaatgc
     1741 catccaaaga ttttttgcat atcatatgat tgagacctat ggatgtgact attccacaag
     1801 tgggctgtct tttgatactc tgcattccaa actgaaagct ttccttgaac ttcggacagt
     1861 ggatggacct agacatgata catatgtttt atattacagt ggacacaccc atggctcagg
     1921 agagtgggcc ctcgcaggtg gagacatact tcgccttgat acgcttttag aatggtggag
     1981 agagaagaac ggttccttct gttcccggct tatcatcatc ttagacagcg agaattcaac
     2041 cccatgggtg aaagaagtga gaaagattaa tgaccagtat gttgcagttc agggagctga
     2101 gctggcgaag acagtagata ttgaagaagc tgacccgcca cagctgggtg actttactag
     2161 agactgggta gaatacaact gtaactccac aaacaacatc tgctggactg agaaggggcg
     2221 cacagtgagg gccgtgtatg gcgtgtcaaa gcgctggagc gactacacgc tgcacctgcc
     2281 gacaggaagc gatgtggcca agcactggat gttacacttc cctcgggtta cgtatccgtt
     2341 agtgcacttg gccaactggt tatgtggcct gaacctcttt tgggtctgca aagcttgttt
     2401 taggtgcttg aaaaggttaa aaatgagttg gtttcttcct acagtgctgg acacaggaca
     2461 aggctttaaa cttgtcaaat cttaatgtgg acatcaagga aggctattat tgagagttca
     2521 tgttaccatt tatcagtaac ttgccatttt ttgtatgctg tatttttatt tgtggaaagt
     2581 atcttgctac atttgtagct gttctcactt tgtcttttct taagtaatta tgggatgtgg
     2641 aagatattgg gaatatgcat gattttatac taaaagtata tggagggtca tgaaaatgct
     2701 gaatgtaaat gtgtaagaga tgttaaaagt aacagtgcac tttcaaataa ctttccattt
     2761 gtctccaatg aaaagaaaca cagttgtctg tgctctggag tggccaatgg acaattccta
     2821 atgccttatt ttctaggcac ccgttagaga atgtagacca gacaagtgtg ttttaagaga
     2881 actaccagtt tacttagtag gagattcttt catgtgtgtg attcatatag gtttggcccc
     2941 aggactgttt gcagaagtat aaatacttgg agcaggaaga gacctgctca taatgactgc
     3001 aaaatgccct tttaccacca cacccagatg tcttggctat gacctttaca gtacagcaga
     3061 aaagccccaa ctgcctttga gatgaaagca ttgttaatgt aggtaacaat aagagcttac
     3121 atgctacaaa gtgttccttc atggttagtg agggactttg aaaatactgg taaatttaag
     3181 agtccttctc agggtaattt tgttttctta atgagtagta tttccagcta caaattcctt
     3241 tcaagtaaat tgccattctt ttcaaaaagt acacacttac aaaaataaat gtatcagttt
     3301 ctcattgacc gattcttcct gtcttacgta tttatgagga gttagtactc tgccatgaaa
     3361 gttgcttttc agtcctattg taataacttt tttctgctga aggtcaagga tgggaacaga
     3421 tcattttgtt gttgttgttg ttatatacct gtattgtatt atgtaaaaaa gtattcattt
     3481 tggcagtttt aggtaagcat aatggtatgg ttgtaatttg taaactttaa ttcagtgttt
     3541 tgttggagta aagtaggcgc tgatcaggat atctccttaa gaggtaagtc acttcctgtc
     3601 cccttccaat tatccttaca ttctagattt tcatctttga aagagggagg tataattaaa
     3661 gtggagatta gactttgtta ccatggtgac ttctcaccgt tgaagccatt tttccatgcc
     3721 cagagaggga aggtaacgga tgcatctacc attttctacc aatgacttga aaactgacat
     3781 tttatttaga aagaagtcac accatcaggg aacttgtata ctgttatcta tgcagcattg
     3841 ctacagccta tttctatgtt ttgaatattg tactcttaat actttgaata atattttgtt
     3901 aaaaattaat ttttcattta atgtgaaatt aagagttcct gaatatttta ctgtattaaa
     3961 agtggaagtt atacaaaatg cctttgcttt tagagtttat gtttggtggc atgttgattt
     4021 gaaaatattt ccttattcca gaatactgaa gaattgatac aatattgaaa gttagtggca
     4081 tcctatatac aatatttttt ttaaaaaatg aatcttgttt tacaactaga aatacagata
     4141 gtaaatttca ctgaataaat cattcatttg tgtgcatctt gaatttcaga tgggtgccat
     4201 ttcacatcag ttttgtataa tttttaggtt cttcaggtcc acaattaaat aaggaaacta
     4261 gcgttgagat gttaccttgc tgtgcacata tctgtgtatc aggagctagg ctcccctacc
     4321 taagtggaaa ggcattgctc tctctagctc taatcctaca attcctttct tcctttccat
     4381 atgaaacaac tctatacaca gccatgggta ttgtctatga tctaattatt ctaagtattt
     4441 ttctgtgatt gattttatta caccttccca aatgctctta ataatttaac atagaaattt
     4501 gtattttata aattatgaaa ctaaggcagt ggactcttac aaaaatgaca gaataataaa
     4561 ctgctctgaa gctaatttga gac
//
