LOCUS       NM_027215                868 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus transmembrane protein 147 (Tmem147), mRNA.
ACCESSION   NM_027215
VERSION     NM_027215.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 868)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 868)
  AUTHORS   Georgas,K.M., Chiu,H.S., Lesieur,E., Rumballe,B.A. and Little,M.H.
  TITLE     Expression of metanephric nephron-patterning genes in
            differentiating mesonephric tubules
  JOURNAL   Dev Dyn 240 (6), 1600-1612 (2011)
   PUBMED   21491542
REFERENCE   3  (bases 1 to 868)
  AUTHORS   Combes,A.N., Lesieur,E., Harley,V.R., Sinclair,A.H., Little,M.H.,
            Wilhelm,D. and Koopman,P.
  TITLE     Three-dimensional visualization of testis cord morphogenesis, a
            novel tubulogenic mechanism in development
  JOURNAL   Dev Dyn 238 (5), 1033-1041 (2009)
   PUBMED   19334288
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK160267.1 and AK010168.1.
            
            On Jan 5, 2007 this sequence version replaced NM_027215.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK010168.1, BB610347.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849377, SAMN00849386
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-96                AK160267.1         3-98
            97-868              AK010168.1         86-857
FEATURES             Location/Qualifiers
     source          1..868
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="7"
                     /map="7 19.23 cM"
     gene            1..868
                     /gene="Tmem147"
                     /gene_synonym="2010004E11Rik; 5033425B17Rik; Nifie14"
                     /note="transmembrane protein 147"
                     /db_xref="GeneID:69804"
                     /db_xref="MGI:MGI:1915011"
     exon            1..191
                     /gene="Tmem147"
                     /gene_synonym="2010004E11Rik; 5033425B17Rik; Nifie14"
                     /inference="alignment:Splign:2.1.0"
     CDS             115..789
                     /gene="Tmem147"
                     /gene_synonym="2010004E11Rik; 5033425B17Rik; Nifie14"
                     /note="seven transmembrane domain protein"
                     /codon_start=1
                     /product="BOS complex subunit TMEM147"
                     /protein_id="NP_081491.2"
                     /db_xref="CCDS:CCDS39890.1"
                     /db_xref="GeneID:69804"
                     /db_xref="MGI:MGI:1915011"
                     /translation="MTLFHFGNCFALAYFPYFITYKCSGLSEYNAFWKCVQAGVTYLF
                     VQLCKMLFLATFFPTWEGGIYDFIGEFMKASVDVADLIGLNLVMSRNAGKGEYKIMVA
                     ALGWATAELIMSRCIPLWVGARGIEFDWKYIQMSIDSNISLVHYIVASAQVWMITRYD
                     LYHTFRPAVLLLMFLSVYKAFVMETFVHLFSLGSWTALLARAVVTGLLALSTLALYVA
                     VVNVHS"
     exon            192..261
                     /gene="Tmem147"
                     /gene_synonym="2010004E11Rik; 5033425B17Rik; Nifie14"
                     /inference="alignment:Splign:2.1.0"
     exon            262..321
                     /gene="Tmem147"
                     /gene_synonym="2010004E11Rik; 5033425B17Rik; Nifie14"
                     /inference="alignment:Splign:2.1.0"
     exon            322..458
                     /gene="Tmem147"
                     /gene_synonym="2010004E11Rik; 5033425B17Rik; Nifie14"
                     /inference="alignment:Splign:2.1.0"
     exon            459..543
                     /gene="Tmem147"
                     /gene_synonym="2010004E11Rik; 5033425B17Rik; Nifie14"
                     /inference="alignment:Splign:2.1.0"
     exon            544..665
                     /gene="Tmem147"
                     /gene_synonym="2010004E11Rik; 5033425B17Rik; Nifie14"
                     /inference="alignment:Splign:2.1.0"
     exon            666..868
                     /gene="Tmem147"
                     /gene_synonym="2010004E11Rik; 5033425B17Rik; Nifie14"
                     /inference="alignment:Splign:2.1.0"
     regulatory      846..851
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Tmem147"
                     /gene_synonym="2010004E11Rik; 5033425B17Rik; Nifie14"
                     /note="hexamer: AGTAAA"
     polyA_site      868
                     /gene="Tmem147"
                     /gene_synonym="2010004E11Rik; 5033425B17Rik; Nifie14"
                     /note="major polyA site"
ORIGIN      
        1 gcgcgcgggc cacggcaaag caggtgggcg ccaggccccg gtgccggcga ggccgactag
       61 aggagacgct gcgcctggcg agtcccgcat ttgcgggacc gggcggctgg cgtcatgacc
      121 ctgttccact tcgggaactg cttcgccctc gcctactttc cctatttcat cacgtacaaa
      181 tgcagcggcc tgtctgaata caacgccttc tggaaatgcg tccaggccgg ggtcacctac
      241 ctctttgtgc agctatgcaa gatgttgttc ttggccactt tcttccccac ctgggaaggt
      301 ggcatctatg acttcattgg ggaattcatg aaggccagtg tggatgtggc agacctgata
      361 ggcctaaacc ttgtcatgtc tcggaatgca ggcaaggggg agtacaagat tatggttgct
      421 gccctgggct gggccactgc tgaactcatt atgtcccgat gcatccccct ctgggtggga
      481 gcccgaggca ttgaatttga ctggaaatat atccagatga gcattgactc caacatcagt
      541 ctggtccact acatcgttgc atcagctcag gtctggatga taacacgcta tgacctgtac
      601 cacaccttcc ggcctgctgt tctcctcctg atgttcctta gtgtctacaa ggcctttgtc
      661 atggagacct tcgtccacct cttctccttg ggtagttgga cagcactgct cgcccgggca
      721 gtagtaacag gactgctggc cctcagcacc ttggccctgt atgttgctgt tgtcaatgta
      781 cactcctagc ttgacatctc aggtgttggc gaaccttttc atttcctctc tccagatttc
      841 agtgaagtaa acagtgtttg gaaaggtt
//
