LOCUS       NM_001403253            2015 bp    mRNA    linear   ROD 06-AUG-2024
DEFINITION  Mus musculus transmembrane p24 trafficking protein 2 (Tmed2),
            transcript variant 1, mRNA.
ACCESSION   NM_001403253 XM_036165330
VERSION     NM_001403253.1
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2015)
  AUTHORS   Sun,C., Zhang,Y., Wang,Z., Chen,J., Zhang,J. and Gu,Y.
  TITLE     TMED2 promotes glioma tumorigenesis by being involved in EGFR
            recycling transport
  JOURNAL   Int J Biol Macromol 262 (Pt 2), 130055 (2024)
   PUBMED   38354922
  REMARK    GeneRIF: TMED2 promotes glioma tumorigenesis by being involved in
            EGFR recycling transport.
REFERENCE   2  (bases 1 to 2015)
  AUTHORS   Montanes-Agudo,P., Aufiero,S., Schepers,E.N., van der Made,I.,
            Cocera-Ortega,L., Ernault,A.C., Richard,S., Kuster,D.W.D.,
            Christoffels,V.M., Pinto,Y.M. and Creemers,E.E.
  TITLE     The RNA-binding protein QKI governs a muscle-specific alternative
            splicing program that shapes the contractile function of
            cardiomyocytes
  JOURNAL   Cardiovasc Res 119 (5), 1161-1174 (2023)
   PUBMED   36627242
REFERENCE   3  (bases 1 to 2015)
  AUTHORS   Di Minin,G., Holzner,M., Grison,A., Dumeau,C.E., Chan,W.,
            Monfort,A., Jerome-Majewska,L.A., Roelink,H. and Wutz,A.
  TITLE     TMED2 binding restricts SMO to the ER and Golgi compartments
  JOURNAL   PLoS Biol 20 (3), e3001596 (2022)
   PUBMED   35353806
  REMARK    GeneRIF: TMED2 binding restricts SMO to the ER and Golgi
            compartments.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2015)
  AUTHORS   Modzelewski,A.J., Shao,W., Chen,J., Lee,A., Qi,X., Noon,M.,
            Tjokro,K., Sales,G., Biton,A., Anand,A., Speed,T.P., Xuan,Z.,
            Wang,T., Risso,D. and He,L.
  TITLE     A mouse-specific retrotransposon drives a conserved Cdk2ap1 isoform
            essential for development
  JOURNAL   Cell 184 (22), 5541-5558 (2021)
   PUBMED   34644528
REFERENCE   5  (bases 1 to 2015)
  AUTHORS   Maurel,M., Obacz,J., Avril,T., Ding,Y.P., Papadodima,O., Treton,X.,
            Daniel,F., Pilalis,E., Horberg,J., Hou,W., Beauchamp,M.C.,
            Tourneur-Marsille,J., Cazals-Hatem,D., Sommerova,L., Samali,A.,
            Tavernier,J., Hrstka,R., Dupont,A., Fessart,D., Delom,F.,
            Fernandez-Zapico,M.E., Jansen,G., Eriksson,L.A., Thomas,D.Y.,
            Jerome-Majewska,L., Hupp,T., Chatziioannou,A., Chevet,E. and
            Ogier-Denis,E.
  TITLE     Control of anterior GRadient 2 (AGR2) dimerization links
            endoplasmic reticulum proteostasis to inflammation
  JOURNAL   EMBO Mol Med 11 (6) (2019)
   PUBMED   31040128
REFERENCE   6  (bases 1 to 2015)
  AUTHORS   Hou,W. and Jerome-Majewska,L.A.
  TITLE     TMED2/emp24 is required in both the chorion and the allantois for
            placental labyrinth layer development
  JOURNAL   Dev Biol 444 (1), 20-32 (2018)
   PUBMED   30236446
REFERENCE   7  (bases 1 to 2015)
  AUTHORS   Hou,W., Gupta,S., Beauchamp,M.C., Yuan,L. and Jerome-Majewska,L.A.
  TITLE     Non-alcoholic fatty liver disease in mice with heterozygous
            mutation in TMED2
  JOURNAL   PLoS One 12 (8), e0182995 (2017)
   PUBMED   28797121
  REMARK    GeneRIF: Histological and molecular analysis of livers from
            heterozygous mice carrying the 99J mutation, Tmed299J/+, revealed a
            requirement for TMED2 in liver health. The study shows that
            Tmed299J/+ mice had decreased levels of TMED2 and TMED10, dilated
            endoplasmic reticulum membrane, and increased phosphorylation of
            eIF2alpha, indicating endoplasmic reticulum - stress.
            Publication Status: Online-Only
REFERENCE   8  (bases 1 to 2015)
  AUTHORS   Xiong,X., Lu,Y., Zhang,L., Wang,B., Zhao,Y., Wang,X.J., Huo,X.,
            Shen,Y., Liang,Z. and Chen,M.
  TITLE     Discovery of novel cell proliferation-enhancing gene by random
            siRNA library based combinatorial screening
  JOURNAL   Comb Chem High Throughput Screen 13 (9), 798-806 (2010)
   PUBMED   20712584
  REMARK    GeneRIF: Tmed2, which has never been reported yet in cell
            proliferation, was verified to be able to enhance MC3T3-E1 cell
            proliferation when over-expressed
REFERENCE   9  (bases 1 to 2015)
  AUTHORS   Jerome-Majewska,L.A., Achkar,T., Luo,L., Lupu,F. and Lacy,E.
  TITLE     The trafficking protein Tmed2/p24beta(1) is required for
            morphogenesis of the mouse embryo and placenta
  JOURNAL   Dev Biol 341 (1), 154-166 (2010)
   PUBMED   20178780
  REMARK    GeneRIF: Study finds that Tmed2/p24beta(1) is normally expressed in
            tissues showing morphological defects in 99J mutant embryos and
            that these affected tissues lack the TMED2/p24beta(1)
            oligomerization partners, TMED7/p24gamma(3) and TMED10/p24delta(1).
REFERENCE   10 (bases 1 to 2015)
  AUTHORS   Chen,F., Hasegawa,H., Schmitt-Ulms,G., Kawarai,T., Bohm,C.,
            Katayama,T., Gu,Y., Sanjo,N., Glista,M., Rogaeva,E., Wakutani,Y.,
            Pardossi-Piquard,R., Ruan,X., Tandon,A., Checler,F., Marambaud,P.,
            Hansen,K., Westaway,D., St George-Hyslop,P. and Fraser,P.
  TITLE     TMP21 is a presenilin complex component that modulates
            gamma-secretase but not epsilon-secretase activity
  JOURNAL   Nature 440 (7088), 1208-1212 (2006)
   PUBMED   16641999
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC127313.3.
            
            On Mar 30, 2022 this sequence version replaced XM_036165330.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR12282455.43973601.1,
                                           SRR5189679.70773.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849375, SAMN00849383
                                           [ECO:0000348]
            ##Evidence-Data-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-270               AC127313.3         116956-117225
            271-463             AC127313.3         119156-119348
            464-484             AC127313.3         119671-119691
            485-592             AC127313.3         123080-123187
            593-2015            AC127313.3         125265-126687
FEATURES             Location/Qualifiers
     source          1..2015
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="5"
                     /map="5 63.65 cM"
     gene            1..2015
                     /gene="Tmed2"
                     /gene_synonym="1110032D12Rik; 1810020N21Rik; p24beta1;
                     Rnp24; Sid394"
                     /note="transmembrane p24 trafficking protein 2"
                     /db_xref="GeneID:56334"
                     /db_xref="MGI:MGI:1929269"
     exon            1..270
                     /gene="Tmed2"
                     /gene_synonym="1110032D12Rik; 1810020N21Rik; p24beta1;
                     Rnp24; Sid394"
                     /inference="alignment:Splign:2.1.0"
     CDS             91..717
                     /gene="Tmed2"
                     /gene_synonym="1110032D12Rik; 1810020N21Rik; p24beta1;
                     Rnp24; Sid394"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1; Sid394p; transmembrane emp24 domain-containing
                     protein 2; sid 394; membrane protein p24A; coated vesicle
                     membrane protein; p24 family protein beta-1; COPI-coated
                     vesicle membrane protein p24; transmembrane emp24 domain
                     trafficking protein 2"
                     /codon_start=1
                     /product="transmembrane emp24 domain-containing protein 2
                     isoform 1 precursor"
                     /protein_id="NP_001390182.1"
                     /db_xref="GeneID:56334"
                     /db_xref="MGI:MGI:1929269"
                     /translation="MVTLAELLALLAALLATASGYFVSIDAHAEECFFERVTSGTKMG
                     LIFEVAEGGFLDIDVEITGPDNKGIYKGDRESSGKYTFAAHMDGTYKFCFSNRMSTMT
                     PKIVMFTIDIGEAPKGQDMETEGGGDSWDAHQNKLEEMINELAVAMTAVKHEQEYMEV
                     RERIHRAINDNTNSRVVLWSFFEALVLVAMTLGQIYYLKRFFEVRRVV"
     exon            271..463
                     /gene="Tmed2"
                     /gene_synonym="1110032D12Rik; 1810020N21Rik; p24beta1;
                     Rnp24; Sid394"
                     /inference="alignment:Splign:2.1.0"
     exon            464..484
                     /gene="Tmed2"
                     /gene_synonym="1110032D12Rik; 1810020N21Rik; p24beta1;
                     Rnp24; Sid394"
                     /inference="alignment:Splign:2.1.0"
     exon            485..592
                     /gene="Tmed2"
                     /gene_synonym="1110032D12Rik; 1810020N21Rik; p24beta1;
                     Rnp24; Sid394"
                     /inference="alignment:Splign:2.1.0"
     exon            593..2015
                     /gene="Tmed2"
                     /gene_synonym="1110032D12Rik; 1810020N21Rik; p24beta1;
                     Rnp24; Sid394"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1992..1997
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Tmed2"
                     /gene_synonym="1110032D12Rik; 1810020N21Rik; p24beta1;
                     Rnp24; Sid394"
                     /note="hexamer: AATAAA"
     polyA_site      2015
                     /gene="Tmed2"
                     /gene_synonym="1110032D12Rik; 1810020N21Rik; p24beta1;
                     Rnp24; Sid394"
                     /note="major polyA site"
ORIGIN      
        1 ggagctgagg cggcgggcgg ctgaggcggc agcggtggcg gcggctccgg aggccgcagt
       61 ccgcgtcccg gcgtcggccc gtcccgcacc atggtgacgc tcgccgagct gctggcgctg
      121 ctggccgcgc tgctggccac ggcctcgggc tactttgtca gcatcgacgc gcacgccgag
      181 gagtgcttct tcgagcgggt cacctccggc accaagatgg gcctcatctt cgaggtggcg
      241 gagggcggct tcctggacat cgacgtggag atcacaggac cagataataa aggaatctat
      301 aaaggagacc gggagtccag cgggaagtac acatttgcag cccacatgga tgggacatac
      361 aagttctgct ttagcaatag gatgtccact atgactccaa agatagtaat gttcaccatt
      421 gacattgggg aggctcccaa aggacaagac atggagacag aaggtggtgg agactcctgg
      481 gatgctcatc agaacaagct agaagaaatg attaatgagc tggcagtggc aatgacagcc
      541 gtaaagcacg aacaggagta catggaagtc cgggagagaa tacacagagc catcaatgac
      601 aacacaaaca gcagagtggt cctttggtcc ttcttcgaag ctcttgttct agttgccatg
      661 acattgggac agatctacta cctgaagaga ttttttgaag tccggagggt tgtttaaaag
      721 gccttttcct gttgatccca aattcatgat ttacttgttt accaaacacc ttggtcataa
      781 taatgtcatt agtttataca tttttatttt ctgaacgttc atgacttatg tttctttgtg
      841 attagtaggt gttggggaaa acctgtttaa gagagttaga gtgaaaattt gacatttgat
      901 tggcctataa ttcttacttg aaaggagccc cattatatag gcccttccaa gaatccagag
      961 gctgttaatg ctgtatggga ctatggtctt tattatgttt ttacctttgg ttttcataat
     1021 aaaatgcagt tcatccagga agtagcccac agcctgacaa tacttgttaa ctaactgcat
     1081 tctagtagtc ccttggttca tcatcagagg ctcattttgt tattacagca acagcaatct
     1141 gaatcttgac cctgtggccc cgcctcaggg tggagagtcc tgatggggcc tgttaccagg
     1201 gtgggcactc atgctttctc ttgagctcga tttaaagact catcaaaact cttcccccta
     1261 aagttttata ttgaacctta gaactgaatc acctctaatt gaattgaaca agcagccaat
     1321 aacttagccg ttctttgaat ccagtttccc aggtgttgga acagactgct gtcctgtgtg
     1381 ctgcagagtc tgtcccttgt tggcacttag gaagatccag tttctccctc actggaagtc
     1441 tgcacttctc aagcagaagg attaagcctc tggggttgct tgcagtgata ggcagccact
     1501 gatgtctttt tagtcagtgg cctttgggcc ctggtcttca gtgtcagcac tagggtttgt
     1561 ttgtcctctg gggtcttttt tggcacatgt cagttttgtt tatataaaat gaagagactg
     1621 tctctctgat gatgggttta atgaaaggca ctctgctttg ggtgtgggcg gtccaggatt
     1681 atgtggtaac tattgtctgt tagttacctg gatattttat ttttcttttt ggatcttgca
     1741 aggggaaaac tacaagtaac aagttttata aatttaattt aaattgttac aggttttcat
     1801 gttcaggaca aataattttt caaccttcga tgatatcctt gcagcagttt gttggtaagg
     1861 tgactttcac ctagggcagc tgttgcatgc caattttttt tttttgtgtg tgtgtgtgaa
     1921 aacacttcag aattgaatta aaagatgtaa atttaaaatt ggttgtgtat ctaatatgcc
     1981 caggttcagt aaataaacaa ttctttttaa aaaca
//
