LOCUS       NM_001355572            1112 bp    mRNA    linear   ROD 02-MAY-2024
DEFINITION  Mus musculus transmembrane and coiled-coil domains 5 (Tmco5),
            transcript variant 2, mRNA.
ACCESSION   NM_001355572 XM_006500077
VERSION     NM_001355572.2
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1112)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 1112)
  AUTHORS   Yamase,K., Tanigawa,Y., Yamamoto,Y., Tanaka,H. and Komiya,T.
  TITLE     Mouse TMCO5 is localized to the manchette microtubules involved in
            vesicle transfer in the elongating spermatids
  JOURNAL   PLoS One 14 (8), e0220917 (2019)
   PUBMED   31393949
  REMARK    GeneRIF: Tmco5 may have a role in vesicle transport along the
            manchette.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 1112)
  AUTHORS   Miyata,H., Castaneda,J.M., Fujihara,Y., Yu,Z., Archambeault,D.R.,
            Isotani,A., Kiyozumi,D., Kriseman,M.L., Mashiko,D., Matsumura,T.,
            Matzuk,R.M., Mori,M., Noda,T., Oji,A., Okabe,M.,
            Prunskaite-Hyyrylainen,R., Ramirez-Solis,R., Satouh,Y., Zhang,Q.,
            Ikawa,M. and Matzuk,M.M.
  TITLE     Genome engineering uncovers 54 evolutionarily conserved and
            testis-enriched genes that are not required for male fertility in
            mice
  JOURNAL   Proc Natl Acad Sci U S A 113 (28), 7704-7710 (2016)
   PUBMED   27357688
REFERENCE   4  (bases 1 to 1112)
  AUTHORS   Koscielny,G., Yaikhom,G., Iyer,V., Meehan,T.F., Morgan,H.,
            Atienza-Herrero,J., Blake,A., Chen,C.K., Easty,R., Di Fenza,A.,
            Fiegel,T., Grifiths,M., Horne,A., Karp,N.A., Kurbatova,N.,
            Mason,J.C., Matthews,P., Oakley,D.J., Qazi,A., Regnart,J.,
            Retha,A., Santos,L.A., Sneddon,D.J., Warren,J., Westerberg,H.,
            Wilson,R.J., Melvin,D.G., Smedley,D., Brown,S.D., Flicek,P.,
            Skarnes,W.C., Mallon,A.M. and Parkinson,H.
  TITLE     The International Mouse Phenotyping Consortium Web Portal, a
            unified point of access for knockout mice and related phenotyping
            data
  JOURNAL   Nucleic Acids Res 42 (Database issue), D802-D809 (2014)
   PUBMED   24194600
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL845318.8.
            
            On Mar 30, 2020 this sequence version replaced NM_001355572.1.
            
            Transcript Variant: This variant (2) lacks an alternate in-frame
            exon compared to variant 1. The resulting isoform (2) has the same
            N- and C-termini but is shorter compared to isoform 1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK007251.1, BY707353.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849384 [ECO:0000348]
            ##Evidence-Data-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-63                AL845318.8         76040-76102
            64-107              AL845318.8         76610-76653
            108-257             AL845318.8         77571-77720
            258-381             AL845318.8         78131-78254
            382-432             AL845318.8         79800-79850
            433-504             AL845318.8         80550-80621
            505-561             AL845318.8         83585-83641
            562-626             AL845318.8         84573-84637
            627-684             AL845318.8         85589-85646
            685-725             AL845318.8         87440-87480
            726-1112            AL845318.8         89448-89834
FEATURES             Location/Qualifiers
     source          1..1112
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 59.03 cM"
     gene            1..1112
                     /gene="Tmco5"
                     /gene_synonym="1700095F04Rik; Tmco5a"
                     /note="transmembrane and coiled-coil domains 5"
                     /db_xref="GeneID:67356"
                     /db_xref="MGI:MGI:1914606"
     exon            1..63
                     /gene="Tmco5"
                     /gene_synonym="1700095F04Rik; Tmco5a"
                     /inference="alignment:Splign:2.1.0"
     exon            64..107
                     /gene="Tmco5"
                     /gene_synonym="1700095F04Rik; Tmco5a"
                     /inference="alignment:Splign:2.1.0"
     CDS             73..924
                     /gene="Tmco5"
                     /gene_synonym="1700095F04Rik; Tmco5a"
                     /note="isoform 2 is encoded by transcript variant 2;
                     transmembrane and coiled-coil domain-containing protein
                     5A"
                     /codon_start=1
                     /product="transmembrane and coiled-coil domain-containing
                     protein 5A isoform 2"
                     /protein_id="NP_001342501.1"
                     /db_xref="GeneID:67356"
                     /db_xref="MGI:MGI:1914606"
                     /translation="MEEQKENQLDYESEKMEILRLAQSKKNIISLNMDLERDMQRIDE
                     ANQELLLEIQEKENEIQRLEHEITQTGNPAEDEEWEKENYTVMEREQALQELEEETAR
                     LERKNETLVHSISELQRKLTRKSQKVIRYEQGDLETTPEESKVKLQQLESSCADQEKE
                     LGKKYQEALKRIEEELETGYLEREVSKVLSMDSERERSTSLNKMDGFISKGALRFSKS
                     IFRSLLFSTLFFIRLLGYLIFHLSFINPDLLVNALPKILSRDVLWKLRCFLFPSLTLE
                     TEDMLPH"
     exon            108..257
                     /gene="Tmco5"
                     /gene_synonym="1700095F04Rik; Tmco5a"
                     /inference="alignment:Splign:2.1.0"
     exon            258..381
                     /gene="Tmco5"
                     /gene_synonym="1700095F04Rik; Tmco5a"
                     /inference="alignment:Splign:2.1.0"
     exon            382..432
                     /gene="Tmco5"
                     /gene_synonym="1700095F04Rik; Tmco5a"
                     /inference="alignment:Splign:2.1.0"
     exon            433..504
                     /gene="Tmco5"
                     /gene_synonym="1700095F04Rik; Tmco5a"
                     /inference="alignment:Splign:2.1.0"
     exon            505..561
                     /gene="Tmco5"
                     /gene_synonym="1700095F04Rik; Tmco5a"
                     /inference="alignment:Splign:2.1.0"
     exon            562..626
                     /gene="Tmco5"
                     /gene_synonym="1700095F04Rik; Tmco5a"
                     /inference="alignment:Splign:2.1.0"
     exon            627..684
                     /gene="Tmco5"
                     /gene_synonym="1700095F04Rik; Tmco5a"
                     /inference="alignment:Splign:2.1.0"
     exon            685..725
                     /gene="Tmco5"
                     /gene_synonym="1700095F04Rik; Tmco5a"
                     /inference="alignment:Splign:2.1.0"
     exon            726..1112
                     /gene="Tmco5"
                     /gene_synonym="1700095F04Rik; Tmco5a"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1092..1097
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Tmco5"
                     /gene_synonym="1700095F04Rik; Tmco5a"
                     /note="hexamer: AGTAAA"
     polyA_site      1112
                     /gene="Tmco5"
                     /gene_synonym="1700095F04Rik; Tmco5a"
                     /note="major polyA site"
ORIGIN      
        1 agagtcttaa gaagaagctc ctgattaggg caggatcaga aagccaaagc acatcggtag
       61 aaggggcctc cgatggaaga acagaaggaa aatcagctgg actatgagtc agaaaaaatg
      121 gaaatcttga gattggccca atcaaagaag aacattatca gcttgaacat ggacctcgaa
      181 agggatatgc agagaataga tgaagccaac caggagctgc ttctcgagat acaagaaaaa
      241 gaaaatgaaa tccagaggct agaacatgag atcacccaga ctgggaaccc tgcagaagac
      301 gaggagtggg agaaggaaaa ctatactgtg atggaaagag aacaagctct gcaggagctg
      361 gaggaagaaa cagccagatt ggagaggaag aatgagactc tggtccacag tatttcagaa
      421 cttcagagaa agcttacaag aaaatcacaa aaggtaatca ggtatgaaca aggtgatcta
      481 gaaacaaccc cagaagagtc caaggttaaa ctacaacagt tggagtcttc ctgtgcagac
      541 caggagaagg agctcggcaa gaagtaccag gaagctttga agaggataga agaagaacta
      601 gaaactggat accttgagag agaagtatca aaagtcttaa gcatggattc tgagagagaa
      661 aggagtacga gcctaaataa gatggatggc ttcatcagta aaggggcact gaggttcagt
      721 aaaagtattt ttcgttccct cttgttctcc acgctgtttt tcatcagatt gctgggatac
      781 ctgatcttcc atttaagttt tataaatccc gacctcctgg tcaacgcgct gcctaagata
      841 ctgagcaggg acgtcttgtg gaagctgaga tgtttcctct tcccatctct cacgcttgag
      901 acagaagaca tgttacctca ctgatcccca agccatacct gagggcagcg gaaggcagga
      961 gggatgaagg cttgcaaatg ggaagtggca aaatctgtgg aggggagaag tgtgtctcag
     1021 ctcttccctc gaggttggcc tacttagtga ccatctgtct cagtgtcacg tttccaggat
     1081 taaccttgcc cagtaaagaa ctctgtcaat aa
//
