LOCUS       NM_181856               2686 bp    mRNA    linear   ROD 09-FEB-2025
DEFINITION  Mus musculus transmembrane channel-like gene family 8 (Tmc8),
            transcript variant C, mRNA.
ACCESSION   NM_181856
VERSION     NM_181856.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2686)
  AUTHORS   Torres,A.D., King,R.E., Uberoi,A., Buehler,D., Yoshida,S.,
            Ward-Shaw,E. and Lambert,P.F.
  TITLE     Deficiency in Ever2 does not increase susceptibility of mice to
            pathogenesis by the mouse papillomavirus, MmuPV1
  JOURNAL   J Virol 98 (7), e0017424 (2024)
   PUBMED   38869286
  REMARK    GeneRIF: Deficiency in Ever2 does not increase susceptibility of
            mice to pathogenesis by the mouse papillomavirus, MmuPV1.
REFERENCE   2  (bases 1 to 2686)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   3  (bases 1 to 2686)
  AUTHORS   Wu,C.J., Li,X., Sommers,C.L., Kurima,K., Huh,S., Bugos,G., Dong,L.,
            Li,W., Griffith,A.J. and Samelson,L.E.
  TITLE     Expression of a TMC6-TMC8-CIB1 heterotrimeric complex in
            lymphocytes is regulated by each of the components
  JOURNAL   J Biol Chem 295 (47), 16086-16099 (2020)
   PUBMED   32917726
  REMARK    GeneRIF: Expression of a TMC6-TMC8-CIB1 heterotrimeric complex in
            lymphocytes is regulated by each of the components.
REFERENCE   4  (bases 1 to 2686)
  AUTHORS   Kurima,K., Yang,Y., Sorber,K. and Griffith,A.J.
  TITLE     Characterization of the transmembrane channel-like (TMC) gene
            family: functional clues from hearing loss and epidermodysplasia
            verruciformis
  JOURNAL   Genomics 82 (3), 300-308 (2003)
   PUBMED   12906855
REFERENCE   5  (bases 1 to 2686)
  AUTHORS   Keresztes,G., Mutai,H. and Heller,S.
  TITLE     TMC and EVER genes belong to a larger novel family, the TMC gene
            family encoding transmembrane proteins
  JOURNAL   BMC Genomics 4 (1), 24 (2003)
   PUBMED   12812529
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL645856.5.
            
            On Aug 25, 2010 this sequence version replaced NM_181856.1.
            
            Transcript Variant: This variant (C) differs in the 5' UTR, lacks a
            portion of the 5' coding region, initiates translation at a
            downstream start codon and uses an alternate in-frame splice site
            in the 5' coding region, compared to variant A. The resulting
            protein (isoform C) has a distinct N-terminus and is shorter than
            isoform A.
            
            Sequence Note: This RefSeq record was created from genomic sequence
            data to make the sequence consistent with the reference genome
            assembly. The genomic coordinates used for the transcript record
            were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK156764.1, SRR13422590.620801.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-112               AL645856.5         72940-73051
            113-333             AL645856.5         73693-73913
            334-482             AL645856.5         74038-74186
            483-632             AL645856.5         74414-74563
            633-715             AL645856.5         74756-74838
            716-852             AL645856.5         75448-75584
            853-1000            AL645856.5         75763-75910
            1001-1171           AL645856.5         77030-77200
            1172-1311           AL645856.5         77372-77511
            1312-1435           AL645856.5         80859-80982
            1436-1533           AL645856.5         81326-81423
            1534-1717           AL645856.5         81551-81734
            1718-1848           AL645856.5         81871-82001
            1849-2007           AL645856.5         82086-82244
            2008-2086           AL645856.5         82732-82810
            2087-2686           AL645856.5         83181-83780
FEATURES             Location/Qualifiers
     source          1..2686
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="11"
                     /map="11 82.96 cM"
     gene            1..2686
                     /gene="Tmc8"
                     /gene_synonym="Ever2; EVIN2; mFLJ00400"
                     /note="transmembrane channel-like gene family 8"
                     /db_xref="GeneID:217356"
                     /db_xref="MGI:MGI:2669037"
     exon            1..112
                     /gene="Tmc8"
                     /gene_synonym="Ever2; EVIN2; mFLJ00400"
                     /inference="alignment:Splign:2.1.0"
     exon            113..333
                     /gene="Tmc8"
                     /gene_synonym="Ever2; EVIN2; mFLJ00400"
                     /inference="alignment:Splign:2.1.0"
     CDS             173..2341
                     /gene="Tmc8"
                     /gene_synonym="Ever2; EVIN2; mFLJ00400"
                     /note="isoform C is encoded by transcript variant C;
                     epidermodysplasia verruciformis 2; transmembrane
                     channel-like protein 8; epidermodysplasia verruciformis
                     protein 2 homolog; transmembrane channel-like 8"
                     /codon_start=1
                     /product="transmembrane channel-like protein 8 isoform C"
                     /protein_id="NP_862904.1"
                     /db_xref="CCDS:CCDS25689.1"
                     /db_xref="GeneID:217356"
                     /db_xref="MGI:MGI:2669037"
                     /translation="MFRQWSVQSGPAPRRPESQAASEELWEQEVERLCASRTPVRMLP
                     YAMADKRFIRELREPEGVKTTFWQRWHRPRRVARQHLREAEQRLARGFGLWEGALYEI
                     GGLFGTGIQSYFTFLRFLLLLNLLTMLLTACFVLLPLVWLRPPELGPALKLRLQCSSS
                     PLPQSDIPRFHNPLWNILTGRAFNNTYLFYGAYRAGPESSSEYSIRLAYLLSPMVCLL
                     LCFCGILQRMAEGLPQQTLLGQRYRTPLSAKVFSSWDFCIRVWEAATIKKHEISNELK
                     MELEEGRRVELAQQQTRAQKACRLLTYLRTNILIVLLVVGAISAIFWATKYSQDNKEE
                     SLFLVLQYLPPGVISLVNFLGPQLFTVLIQLENYPPGTEVNLTLIWCVVLKLASLGMF
                     SFSLGQTVLCIGRNKTSCESYGYNACDYQCWENSVGEELYKLIIFNFLLTVAFAFLVS
                     LPRRLLVERFSGWFWTWLDREEFLVPKNVLDIVAAQTVTWMGLFYCPLLPLLNSVFLF
                     LTFYIKKYTLLRNSRASPRRFRASSSTFFFHLVLLLGLLLAAVPLAYVISSTHSSWDC
                     GLFTNYSAPWQVVPELVALQLPLPSQRALRYLSSHAFSFPLLILLSIVLTVCISQSRA
                     NARAIQGLRKQLVWQVQEKWHLVDDLSRLLPELSPEPGSPHSRASRPRSFCPGFPCPG
                     SPGPRTPRLAPSNRLSSSSLGAPSASVPASRFHFPSRTEL"
     exon            334..482
                     /gene="Tmc8"
                     /gene_synonym="Ever2; EVIN2; mFLJ00400"
                     /inference="alignment:Splign:2.1.0"
     exon            483..632
                     /gene="Tmc8"
                     /gene_synonym="Ever2; EVIN2; mFLJ00400"
                     /inference="alignment:Splign:2.1.0"
     exon            633..715
                     /gene="Tmc8"
                     /gene_synonym="Ever2; EVIN2; mFLJ00400"
                     /inference="alignment:Splign:2.1.0"
     exon            716..852
                     /gene="Tmc8"
                     /gene_synonym="Ever2; EVIN2; mFLJ00400"
                     /inference="alignment:Splign:2.1.0"
     exon            853..1000
                     /gene="Tmc8"
                     /gene_synonym="Ever2; EVIN2; mFLJ00400"
                     /inference="alignment:Splign:2.1.0"
     exon            1001..1171
                     /gene="Tmc8"
                     /gene_synonym="Ever2; EVIN2; mFLJ00400"
                     /inference="alignment:Splign:2.1.0"
     exon            1172..1311
                     /gene="Tmc8"
                     /gene_synonym="Ever2; EVIN2; mFLJ00400"
                     /inference="alignment:Splign:2.1.0"
     exon            1312..1435
                     /gene="Tmc8"
                     /gene_synonym="Ever2; EVIN2; mFLJ00400"
                     /inference="alignment:Splign:2.1.0"
     exon            1436..1533
                     /gene="Tmc8"
                     /gene_synonym="Ever2; EVIN2; mFLJ00400"
                     /inference="alignment:Splign:2.1.0"
     exon            1534..1717
                     /gene="Tmc8"
                     /gene_synonym="Ever2; EVIN2; mFLJ00400"
                     /inference="alignment:Splign:2.1.0"
     exon            1718..1848
                     /gene="Tmc8"
                     /gene_synonym="Ever2; EVIN2; mFLJ00400"
                     /inference="alignment:Splign:2.1.0"
     exon            1849..2007
                     /gene="Tmc8"
                     /gene_synonym="Ever2; EVIN2; mFLJ00400"
                     /inference="alignment:Splign:2.1.0"
     exon            2008..2086
                     /gene="Tmc8"
                     /gene_synonym="Ever2; EVIN2; mFLJ00400"
                     /inference="alignment:Splign:2.1.0"
     exon            2087..2686
                     /gene="Tmc8"
                     /gene_synonym="Ever2; EVIN2; mFLJ00400"
                     /inference="alignment:Splign:2.1.0"
     polyA_site      2550
                     /gene="Tmc8"
                     /gene_synonym="Ever2; EVIN2; mFLJ00400"
                     /note="major polyA site"
     regulatory      2641..2646
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Tmc8"
                     /gene_synonym="Ever2; EVIN2; mFLJ00400"
                     /note="hexamer: AATATA"
     polyA_site      2658
                     /gene="Tmc8"
                     /gene_synonym="Ever2; EVIN2; mFLJ00400"
                     /note="major polyA site"
ORIGIN      
        1 actccctcac caccctgcct ggtgtgtcaa gagctggtct taggaggggg ctcttcgggg
       61 agcttggtcc cttgcctctt tgtgagctgc ttgccttgtg acaataagga cgggccaagg
      121 tggagctcct gcgagcaagg atcttgcctg tctgtcccgc gcgcccactg ggatgtttcg
      181 ccagtggtcg gtgcagtcag gaccagcccc acgaaggcct gaatcccagg cggcctcgga
      241 ggagctgtgg gagcaagaag tggagcggct atgcgcctcc cggacgcctg tgcggatgct
      301 gccttacgcc atggcagaca agcgcttcat ccgggaactg cgggagcccg agggagtgaa
      361 gaccaccttc tggcagcggt ggcatcgccc gaggcgggtg gcgcgacaac acctgaggga
      421 ggcagagcaa cggctggccc gcggcttcgg tctctgggag ggagctctct acgagattgg
      481 gggcctcttc ggaaccggga tccagtccta cttcactttc cttcgcttcc tgctgctgct
      541 caacctgctg accatgctgc tgactgcctg ctttgtgctg ctgcccctgg tctggctccg
      601 cccccctgag ctaggccctg ccctgaaact gagactccag tgctccagca gccccctgcc
      661 tcagagtgac attccaaggt ttcacaaccc actttggaat attttaacgg gcagagcctt
      721 caacaacacc tatctcttct acggtgccta ccgagcgggg ccagagagca gctctgaata
      781 cagcatccgc ctggcctacc tccttagccc gatggtctgc ctgctcctct gtttctgtgg
      841 gatactgcag aggatggcag aaggcctacc ccagcagaca ctcttgggtc agcgctacag
      901 gacacccctc agcgccaaag tgttctcatc ctgggacttc tgcatccggg tgtgggaagc
      961 cgccaccatc aagaagcacg aaatcagcaa tgaactgaag atggagctgg aagagggccg
     1021 ccgcgtggag ctggctcaac aacagaccag ggcccagaag gcctgccgcc tgctcaccta
     1081 ccttcgcacc aacatcctca ttgtgctcct agtggtcgga gccatcagcg ccatcttctg
     1141 ggccaccaag tactcacagg acaacaagga ggagtccctt tttctggtgc tccagtactt
     1201 gcctcctgga gtcatctccc tggtcaactt cctgggcccc caattgttca cagtcctgat
     1261 ccagctggag aactaccctc ccggcactga agtcaacctc accctgatct ggtgcgtggt
     1321 gctgaagctg gccagcttgg gcatgttctc cttctccctg ggtcagacgg tgctgtgcat
     1381 aggcagaaac aagaccagct gcgagtccta cggctacaac gcttgcgact accagtgctg
     1441 ggagaactcg gtgggcgaag aattatataa gttaatcatc ttcaacttcc tgctcaccgt
     1501 ggccttcgcc ttcctggtca gcctgcctcg aaggctgtta gtggagcgct tctcgggctg
     1561 gttctggact tggctagacc gggaggagtt cctggtgcct aagaatgtgc tggacatcgt
     1621 ggctgcacag actgtcacct ggatgggcct cttctactgc cccctgctgc cactactcaa
     1681 cagtgtcttc ctctttctca ccttctacat caagaagtac accctcctga ggaactccag
     1741 ggcgtccccg cggcgtttcc gagcctccag ctccaccttc ttcttccacc tggtgctgct
     1801 cctaggcctg cttttggctg cagtacctct ggcctacgtg atcagcagca cccactcctc
     1861 ctgggactgt ggcctcttca caaactattc ggccccctgg caggtggtac ccgagctggt
     1921 agccctccag ctcccacttc ctagccagcg tgccctccgt tacctcagct cccacgcctt
     1981 cagcttccca ctcctcattc tgctcagcat cgtcctgacg gtttgcatct cccagtcccg
     2041 ggcgaatgcc agagccatcc aggggctgag gaagcaactg gtgtggcagg tccaggaaaa
     2101 gtggcacctg gtggacgacc tgtcgcggct gctgccagaa ctgagcccag agcccgggtc
     2161 ccctcactct cgagcttcgc gtccacgatc cttctgtcct ggatttccat gcccaggctc
     2221 gcccggtccc aggacaccca ggctggcacc ctccaacagg ctgagctcct cgagcctcgg
     2281 agcccccagt gcctcggttc ctgcctccag atttcacttc cccagccgca cagagctgta
     2341 gcaccgaccc cacgtccctt ggctctgccg cagcttcctc cagtgcctcc tgcactctca
     2401 gcccttccac cacctcctta ggcccacaat ctctgccccc cccccgccgc ccccgcccaa
     2461 accactgcat acagatggct cctggtcctc tagcaaggtt ctccaaagca agccacaccc
     2521 tcaggaagaa catgagaatt tctacctcta ttttatttta tctcattcct tcaatttttt
     2581 tattgagtgt tctatagtgt acataacagt atgaatataa actcatggat gcaatttata
     2641 aatatatata tatatgtata tatttggaaa tacttgaatt tcttgg
//
