LOCUS       NM_181820               2279 bp    mRNA    linear   ROD 04-AUG-2024
DEFINITION  Mus musculus transmembrane channel-like gene family 4 (Tmc4), mRNA.
ACCESSION   NM_181820
VERSION     NM_181820.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2279)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 2279)
  AUTHORS   Narukawa,M., Masago,A., Murata,M., Saito,Y., Kasahara,Y., Abe,K.
            and Asakura,T.
  TITLE     Mouse TMC4 is involved in the detection of chloride taste of salts
  JOURNAL   Biosci Biotechnol Biochem 88 (2), 203-205 (2024)
   PUBMED   37947260
  REMARK    GeneRIF: Mouse TMC4 is involved in the detection of chloride taste
            of salts.
REFERENCE   3  (bases 1 to 2279)
  AUTHORS   Kasahara,Y., Narukawa,M., Ishimaru,Y., Kanda,S., Umatani,C.,
            Takayama,Y., Tominaga,M., Oka,Y., Kondo,K., Kondo,T., Takeuchi,A.,
            Misaka,T., Abe,K. and Asakura,T.
  TITLE     TMC4 is a novel chloride channel involved in high-concentration
            salt taste sensation
  JOURNAL   J Physiol Sci 71 (1), 23 (2021)
   PUBMED   34429071
  REMARK    GeneRIF: TMC4 is a novel chloride channel involved in
            high-concentration salt taste sensation.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2279)
  AUTHORS   Helsley,R.N., Varadharajan,V., Brown,A.L., Gromovsky,A.D.,
            Schugar,R.C., Ramachandiran,I., Fung,K., Kabbany,M.N., Banerjee,R.,
            Neumann,C.K., Finney,C., Pathak,P., Orabi,D., Osborn,L.J.,
            Massey,W., Zhang,R., Kadam,A., Sansbury,B.E., Pan,C., Sacks,J.,
            Lee,R.G., Crooke,R.M., Graham,M.J., Lemieux,M.E., Gogonea,V.,
            Kirwan,J.P., Allende,D.S., Civelek,M., Fox,P.L., Rudel,L.L.,
            Lusis,A.J., Spite,M. and Brown,J.M.
  TITLE     Obesity-linked suppression of membrane-bound O-acyltransferase 7
            (MBOAT7) drives non-alcoholic fatty liver disease
  JOURNAL   Elife 8, e49882 (2019)
   PUBMED   31621579
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2279)
  AUTHORS   Sharov,A.A., Piao,Y., Matoba,R., Dudekula,D.B., Qian,Y.,
            VanBuren,V., Falco,G., Martin,P.R., Stagg,C.A., Bassey,U.C.,
            Wang,Y., Carter,M.G., Hamatani,T., Aiba,K., Akutsu,H., Sharova,L.,
            Tanaka,T.S., Kimber,W.L., Yoshikawa,T., Jaradat,S.A., Pantano,S.,
            Nagaraja,R., Boheler,K.R., Taub,D., Hodes,R.J., Longo,D.L.,
            Schlessinger,D., Keller,J., Klotz,E., Kelsoe,G., Umezawa,A.,
            Vescovi,A.L., Rossant,J., Kunath,T., Hogan,B.L., Curci,A.,
            D'Urso,M., Kelso,J., Hide,W. and Ko,M.S.
  TITLE     Transcriptome analysis of mouse stem cells and early embryos
  JOURNAL   PLoS Biol 1 (3), E74 (2003)
   PUBMED   14691545
REFERENCE   6  (bases 1 to 2279)
  AUTHORS   Kurima,K., Yang,Y., Sorber,K. and Griffith,A.J.
  TITLE     Characterization of the transmembrane channel-like (TMC) gene
            family: functional clues from hearing loss and epidermodysplasia
            verruciformis
  JOURNAL   Genomics 82 (3), 300-308 (2003)
   PUBMED   12906855
REFERENCE   7  (bases 1 to 2279)
  AUTHORS   Keresztes,G., Mutai,H. and Heller,S.
  TITLE     TMC and EVER genes belong to a larger novel family, the TMC gene
            family encoding transmembrane proteins
  JOURNAL   BMC Genomics 4 (1), 24 (2003)
   PUBMED   12812529
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC171680.5.
            
            On Mar 3, 2022 this sequence version replaced NM_181820.2.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC040466.1, AY263162.2 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849377, SAMN01164134
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-100               AC171680.5         90757-90856         c
            101-305             AC171680.5         89897-90101         c
            306-451             AC171680.5         88759-88904         c
            452-684             AC171680.5         88183-88415         c
            685-806             AC171680.5         87920-88041         c
            807-954             AC171680.5         85296-85443         c
            955-1122            AC171680.5         84607-84774         c
            1123-1286           AC171680.5         84271-84434         c
            1287-1413           AC171680.5         83248-83374         c
            1414-1511           AC171680.5         82907-83004         c
            1512-1695           AC171680.5         80774-80957         c
            1696-1826           AC171680.5         80381-80511         c
            1827-1982           AC171680.5         80152-80307         c
            1983-2061           AC171680.5         79435-79513         c
            2062-2279           AC171680.5         79124-79341         c
FEATURES             Location/Qualifiers
     source          1..2279
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="7"
                     /map="7 2.11 cM"
     gene            1..2279
                     /gene="Tmc4"
                     /note="transmembrane channel-like gene family 4"
                     /db_xref="GeneID:353499"
                     /db_xref="MGI:MGI:2669035"
     exon            1..100
                     /gene="Tmc4"
                     /inference="alignment:Splign:2.1.0"
     CDS             46..2130
                     /gene="Tmc4"
                     /note="transmembrane channel-like protein 4"
                     /codon_start=1
                     /product="voltage-gated chloride channel TMC4"
                     /protein_id="NP_861541.2"
                     /db_xref="CCDS:CCDS20723.1"
                     /db_xref="GeneID:353499"
                     /db_xref="MGI:MGI:2669035"
                     /translation="MEAWGQSPACSSSRKARTGPSLASVLNDLPSAATLRYRGPGVLP
                     WGTLDEDDDEGGRSLQAFAETAQMESHPSRELPWPMQARRAHRKSQATGQLASGSESR
                     AAYWTRQLSRTKGKMKEGFQTIQPWAWTLKKIGGQFGAGTESYFSLLRFLLFLNLVAS
                     VIEICMKLIPTWLEGAPPGPPGPNISSPCGSYIPHTHGLVAFPTQLFNLLSGEGYLEW
                     SPLFYGFYPPRSNLAITYLCSVFAISIIYLLCILRRSVSGLKETLLAESDILTSYSHR
                     VFSAWNFGLCGDVHVRLRQRIILYELQVDLEEAVVRRRAAEQTLSQRAKVWSMRALLN
                     VLVLALLGAAFYGIYWATEYTLTLQETPLVRQTPLFKLLVDYLPSIFISLFNFVLPPV
                     FKFIASLEGYTQSRQIVLILLRTVFLRLASLVFLLVSLWSQITCGGNMEAEGCKACGY
                     NYKEIPCWETRLGQEMYKLVLFDLLMGLLVTLLVQFPRKILCGLCPGALGRLSGTLEF
                     QVPDEVLGLIYAQTVVWVGSFFCPLLPLINTAKFLILFCLKKITLFSIYSPASRTFRA
                     STANFFFPLVLLVGLAISAVPVLYSIFLIPPSKLCGPFRGKLSIWAQIPEAIESLPQT
                     AQNFLYFLGTQAFTVPLLILSSILMMYTVALANCYGRLISELKRQIETEVQNKVFLAQ
                     RAVALSSRNGTS"
     exon            101..305
                     /gene="Tmc4"
                     /inference="alignment:Splign:2.1.0"
     exon            306..451
                     /gene="Tmc4"
                     /inference="alignment:Splign:2.1.0"
     exon            452..684
                     /gene="Tmc4"
                     /inference="alignment:Splign:2.1.0"
     exon            685..806
                     /gene="Tmc4"
                     /inference="alignment:Splign:2.1.0"
     exon            807..954
                     /gene="Tmc4"
                     /inference="alignment:Splign:2.1.0"
     exon            955..1122
                     /gene="Tmc4"
                     /inference="alignment:Splign:2.1.0"
     exon            1123..1286
                     /gene="Tmc4"
                     /inference="alignment:Splign:2.1.0"
     exon            1287..1413
                     /gene="Tmc4"
                     /inference="alignment:Splign:2.1.0"
     exon            1414..1511
                     /gene="Tmc4"
                     /inference="alignment:Splign:2.1.0"
     exon            1512..1695
                     /gene="Tmc4"
                     /inference="alignment:Splign:2.1.0"
     exon            1696..1826
                     /gene="Tmc4"
                     /inference="alignment:Splign:2.1.0"
     exon            1827..1982
                     /gene="Tmc4"
                     /inference="alignment:Splign:2.1.0"
     exon            1983..2061
                     /gene="Tmc4"
                     /inference="alignment:Splign:2.1.0"
     exon            2062..2279
                     /gene="Tmc4"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2260..2265
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Tmc4"
                     /note="hexamer: AATAAA"
     polyA_site      2279
                     /gene="Tmc4"
                     /note="major polyA site"
ORIGIN      
        1 agagacagaa agaacgggtg ctggaagtac ggtacatcca caaacatgga agcctggggc
       61 cagtccccag cctgctccag ctcccggaaa gccagaacag gcccatcgct ggcgtctgtg
      121 ctgaacgatc tccccagcgc tgctacgctg cgataccgcg gccctggtgt gctcccttgg
      181 ggaacactgg atgaagatga tgatgaagga ggaaggagcc ttcaggcctt tgcagaaact
      241 gcacaaatgg aatctcaccc ttcccgggaa cttccctggc ctatgcaggc aaggcgggca
      301 cacaggaaga gccaggctac agggcagctg gcttctggct ctgaatccag ggctgcctac
      361 tggacccgac agctttcccg gaccaaggga aaaatgaaag aaggctttca aactatacag
      421 ccctgggcat ggacgctgaa gaaaattggg ggtcagtttg gagcaggcac tgagtcctac
      481 ttctccctgc tgcgcttcct gctctttctc aacctggtgg catctgtcat tgaaatctgc
      541 atgaagctga tccccacctg gctggaaggg gctcctccag gtccccctgg ccctaacatc
      601 tcttcaccct gtggttccta tattccacac actcatggcc tggtcgcctt ccccacccag
      661 cttttcaact tgctctctgg agagggttac cttgagtggt cccctctatt ctatgggttc
      721 tacccacccc gatcgaacct ggccatcacc tacctgtgct ccgtctttgc catcagcatc
      781 atctacttgc tgtgcatcct acgacgttcg gtgtccgggt tgaaggagac actgttggcg
      841 gagtcagaca ttctgaccag ctacagtcac cgagtgttct cagcttggaa ttttggcctc
      901 tgcggggacg tgcacgtgcg gctgcgccag cgcatcattt tgtatgaact acaggtggac
      961 ctagaggaag cagtagtccg gcgccgtgct gcggagcaga ctctgagcca acgagccaag
     1021 gtgtggtcga tgagggcgct gctcaacgtg ctggtcctcg cgctgctcgg ggcggccttc
     1081 tatggcatct actgggccac cgagtacacc ttgacactac aggagacgcc ccttgtccga
     1141 cagaccccac tattcaagct tctggtggat tatctaccat ccatcttcat ttctctgttc
     1201 aactttgtgc tgcctcctgt gttcaagttc attgcctcac tagagggcta cactcaaagc
     1261 cgacagatcg tcttgatcct gcttaggact gtgtttttgc gtctggcctc gctggtgttc
     1321 cttctggtct ctctgtggag tcagatcacg tgtgggggaa acatggaggc agaaggatgc
     1381 aaggcctgcg gctataatta caaagaaatt ccgtgctggg agactcggct gggccaggag
     1441 atgtacaaac ttgtactctt tgatctgctg atgggcctgt tggtcacgct gttggtccag
     1501 tttcctagga agatactctg tggcctctgt cctggggcac tgggtcgtct gtcgggaacc
     1561 ctggagttcc aggtgccaga tgaggtgctg gggctcatct atgcgcagac cgtggtctgg
     1621 gtgggaagct tcttctgccc tttactgcct ttgatcaaca ctgctaagtt cctcatactg
     1681 ttctgcctga agaagataac cctcttctcc atctactcac cggcttcccg caccttccga
     1741 gcatccacgg caaacttctt tttccccctg gttctccttg tgggtctggc gatctctgca
     1801 gttcccgtgc tttacagtat cttcctgatc ccaccttcga aactgtgtgg cccgttccgc
     1861 gggaagctgt ccatttgggc ccagatccct gaggccattg agagcctccc tcagactgcc
     1921 cagaattttc tctactttct gggaacccaa gcttttactg tacctcttct gatcctctct
     1981 agcatcctga tgatgtatac cgtagccctg gctaactgtt acggacgcct catctctgag
     2041 ctcaaacgcc agatagagac ggaagtgcag aataaagtct tcctggccca gcgagctgtg
     2101 gctctgagct caaggaatgg aacttcctga taagtctggc tgaaccttca gcccctcagc
     2161 ttcagtgact tcatatcaag tgatgggact ctttcatctc tggaaccaaa ttccccgccc
     2221 tctgccaccc gaatgctaca ctctcaattg aaccgtatta ataaacaaca gtcaaggcc
//
