LOCUS       NM_021297               7891 bp    mRNA    linear   ROD 26-NOV-2024
DEFINITION  Mus musculus toll-like receptor 4 (Tlr4), mRNA.
ACCESSION   NM_021297
VERSION     NM_021297.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 7891)
  AUTHORS   Pizzuto,M., Hurtado-Navarro,L., Molina-Lopez,C., Soubhye,J.,
            Gelbcke,M., Rodriguez-Lopez,S., Ruysschaert,J.M., Schroder,K. and
            Pelegrin,P.
  TITLE     Ornithine lipid is a partial TLR4 agonist and NLRP3 activator
  JOURNAL   Cell Rep 43 (10), 114788 (2024)
   PUBMED   39340778
REFERENCE   2  (bases 1 to 7891)
  AUTHORS   Kim,Y.S., Choi,S.H., Kim,K.Y., Navia-Pelaez,J.M., Perkins,G.A.,
            Choi,S., Kim,J., Nazarenkov,N., Rissman,R.A., Ju,W.K.,
            Ellisman,M.H. and Miller,Y.I.
  TITLE     AIBP controls TLR4 inflammarafts and mitochondrial dysfunction in a
            mouse model of Alzheimer's disease
  JOURNAL   J Neuroinflammation 21 (1), 245 (2024)
   PUBMED   39342323
  REMARK    GeneRIF: AIBP controls TLR4 inflammarafts and mitochondrial
            dysfunction in a mouse model of Alzheimer's disease.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 7891)
  AUTHORS   Takahashi,T., Asano,Y., Ichimura,Y., Toyama,T., Taniguchi,T.,
            Noda,S., Akamata,K., Tada,Y., Sugaya,M., Kadono,T. and Sato,S.
  TITLE     Amelioration of tissue fibrosis by toll-like receptor 4 knockout in
            murine models of systemic sclerosis
  JOURNAL   Arthritis Rheumatol 67 (1), 254-265 (2015)
   PUBMED   25302613
  REMARK    GeneRIF: There is a pivotal contribution of TLR-4 to the pathologic
            tissue fibrosis of systemic sclerosis murine models.
REFERENCE   4  (bases 1 to 7891)
  AUTHORS   Jialal,I., Major,A.M. and Devaraj,S.
  TITLE     Global Toll-like receptor 4 knockout results in decreased renal
            inflammation, fibrosis and podocytopathy
  JOURNAL   J Diabetes Complications 28 (6), 755-761 (2014)
   PUBMED   25116192
  REMARK    GeneRIF: Global genetic deficiency of TLR4 also ameliorates renal
            inflammation, fibrosis and podocytopathy and could be important in
            diabetic nephropathy.
REFERENCE   5  (bases 1 to 7891)
  AUTHORS   Zanoni,I., Ostuni,R., Marek,L.R., Barresi,S., Barbalat,R.,
            Barton,G.M., Granucci,F. and Kagan,J.C.
  TITLE     CD14 controls the LPS-induced endocytosis of Toll-like receptor 4
  JOURNAL   Cell 147 (4), 868-880 (2011)
   PUBMED   22078883
  REMARK    GeneRIF: These data establish that upon microbial detection, an
            upstream Pattern Recognition Receptor, CD14 controls the
            trafficking and signaling functions of a downstream Pattern
            Recognition Receptor, TLR4.
REFERENCE   6  (bases 1 to 7891)
  AUTHORS   West,A.P., Brodsky,I.E., Rahner,C., Woo,D.K., Erdjument-Bromage,H.,
            Tempst,P., Walsh,M.C., Choi,Y., Shadel,G.S. and Ghosh,S.
  TITLE     TLR signalling augments macrophage bactericidal activity through
            mitochondrial ROS
  JOURNAL   Nature 472 (7344), 476-480 (2011)
   PUBMED   21525932
REFERENCE   7  (bases 1 to 7891)
  AUTHORS   Kim,H.M., Park,B.S., Kim,J.I., Kim,S.E., Lee,J., Oh,S.C.,
            Enkhbayar,P., Matsushima,N., Lee,H., Yoo,O.J. and Lee,J.O.
  TITLE     Crystal structure of the TLR4-MD-2 complex with bound endotoxin
            antagonist Eritoran
  JOURNAL   Cell 130 (5), 906-917 (2007)
   PUBMED   17803912
  REMARK    GeneRIF: Based on structural analysis and mutagenesis experiments
            on MD-2 and TLR4, we propose a model of TLR4-MD-2 dimerization
            induced by LPS.
REFERENCE   8  (bases 1 to 7891)
  AUTHORS   Foster,S.L., Hargreaves,D.C. and Medzhitov,R.
  TITLE     Gene-specific control of inflammation by TLR-induced chromatin
            modifications
  JOURNAL   Nature 447 (7147), 972-978 (2007)
   PUBMED   17538624
  REMARK    GeneRIF: Using an in vitro system of lipopolysaccharide tolerance
            in murine macrophages, we show that TLR-induced genes fall into two
            categories on the basis of their functions and regulatory
            requirements
            Erratum:[Nature. 2008 Jan 3;451(7174):102]
REFERENCE   9  (bases 1 to 7891)
  AUTHORS   Rosenstreich,D.L., Vogel,S.N., Jacques,A.R., Wahl,L.M. and
            Oppenheim,J.J.
  TITLE     Macrophage sensitivity to endotoxin: genetic control by a single
            codominant gene
  JOURNAL   J Immunol 121 (5), 1664-1670 (1978)
   PUBMED   101590
REFERENCE   10 (bases 1 to 7891)
  AUTHORS   Watson,J. and Riblet,R.
  TITLE     Genetic control of responses to bacterial lipopolysaccharides in
            mice. II. A gene that influences a membrane component involved in
            the activation of bone marrow-derived lymphocytes by
            lipipolysaccharides
  JOURNAL   J Immunol 114 (5), 1462-1468 (1975)
   PUBMED   123543
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BX649609.2, CD544295.1,
            JX878359.1, AK030348.1, AI450864.1 and BX514079.1.
            
            On Sep 15, 2015 this sequence version replaced NM_021297.2.
            
            Summary: This gene belongs to the evolutionarily-conserved
            Toll-like receptor family, whose members are type-1 transmembrane
            proteins that are involved in innate immunity. Toll-like receptors
            are characterized by an extracellular leucine-rich repeat domain
            that functions in ligand recognition and an intracellular
            toll/interleukin-1 receptor-like domain that is crucial for signal
            transduction. The receptor encoded by this gene mediates the innate
            immune response to bacterial lipopolysaccharide, a major component
            of the outer membrane of Gram-negative bacteria, through synthesis
            of pro-inflammatory cytokines and chemokines. In addition, this
            protein can recognize other pathogens from Gram-negative and
            Gram-positive bacteria as well as viral components. Mice deficient
            in this gene display a number of immune response-related phenotypes
            including hyporesponsiveness to bacterial lipopolysaccharide and
            increased levels of respiratory syncytial virus compared to
            controls. [provided by RefSeq, Sep 2015].
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK030348.1, AF110133.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-56                BX649609.2         8372-8427
            57-266              CD544295.1         1-210
            267-2789            JX878359.1         1-2523
            2790-3901           AK030348.1         2732-3843
            3902-4108           AI450864.1         1-207               c
            4109-7798           BX649609.2         23620-27309
            7799-7891           BX514079.1         427-519
FEATURES             Location/Qualifiers
     source          1..7891
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="4"
                     /map="4 34.66 cM"
     gene            1..7891
                     /gene="Tlr4"
                     /gene_synonym="Lps; Ly87; Ran/M1; Rasl2-8"
                     /note="toll-like receptor 4"
                     /db_xref="GeneID:21898"
                     /db_xref="MGI:MGI:96824"
     exon            1..371
                     /gene="Tlr4"
                     /gene_synonym="Lps; Ly87; Ran/M1; Rasl2-8"
                     /inference="alignment:Splign:2.1.0"
     exon            372..538
                     /gene="Tlr4"
                     /gene_synonym="Lps; Ly87; Ran/M1; Rasl2-8"
                     /inference="alignment:Splign:2.1.0"
     exon            539..7891
                     /gene="Tlr4"
                     /gene_synonym="Lps; Ly87; Ran/M1; Rasl2-8"
                     /inference="alignment:Splign:2.1.0"
     regulatory      7321..7326
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Tlr4"
                     /gene_synonym="Lps; Ly87; Ran/M1; Rasl2-8"
                     /note="hexamer: AATAAA"
     polyA_site      7342
                     /gene="Tlr4"
                     /gene_synonym="Lps; Ly87; Ran/M1; Rasl2-8"
                     /note="major polyA site"
     regulatory      7377..7382
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Tlr4"
                     /gene_synonym="Lps; Ly87; Ran/M1; Rasl2-8"
                     /note="hexamer: ATTAAA"
     polyA_site      7398
                     /gene="Tlr4"
                     /gene_synonym="Lps; Ly87; Ran/M1; Rasl2-8"
ORIGIN      
        1 agcttcctct tgctgttcct ccagtcggtc agcaaacgcc ttcttcctgt tctagtcttc
       61 agtcttctaa cttccctcct gcgacggggc agatcgattc tagaacaaaa ccaaaagtga
      121 gaatgctaag gttggcactc tcacttcctc tttgaatata gtacttgcag aggggcaccc
      181 actgggaggg aagaggcagg tgtcccaggg actctgcgct gccaccagtt acagatcgtc
      241 atgttctctc atggcctcca ctggttgcag aaaatgccag gatgatgcct ccctggctcc
      301 tggctaggac tctgatcatg gcactgttct tctcctgcct gacaccagga agcttgaatc
      361 cctgcataga ggtagttcct aatattacct accaatgcat ggatcagaaa ctcagcaaag
      421 tccctgatga cattccttct tcaaccaaga acatagatct gagcttcaac cccttgaaga
      481 tcttaaaaag ctatagcttc tccaattttt cagaacttca gtggctggat ttatccaggt
      541 gtgaaattga aacaattgaa gacaaggcat ggcatggctt acaccacctc tcaaacttga
      601 tactgacagg aaaccctatc cagagttttt ccccaggaag tttctctgga ctaacaagtt
      661 tagagaatct ggtggctgtg gagacaaaat tggcctctct agaaagcttc cctattggac
      721 agcttataac cttaaagaaa ctcaatgtgg ctcacaattt tatacattcc tgtaagttac
      781 ctgcatattt ttccaatctg acgaacctag tacatgtgga tctttcttat aactatattc
      841 aaactattac tgtcaacgac ttacagtttc tacgtgaaaa tccacaagtc aatctctctt
      901 tagacatgtc tttgaaccca attgacttca ttcaagacca agcctttcag ggaattaagc
      961 tccatgaact gactctaaga ggtaatttta atagctcaaa tataatgaaa acttgccttc
     1021 aaaacctggc tggtttacac gtccatcggt tgatcttggg agaatttaaa gatgaaagga
     1081 atctggaaat ttttgaaccc tctatcatgg aaggactatg tgatgtgacc attgatgagt
     1141 tcaggttaac atatacaaat gatttttcag atgatattgt taagttccat tgcttggcga
     1201 atgtttctgc aatgtctctg gcaggtgtat ctataaaata tctagaagat gttcctaaac
     1261 atttcaaatg gcaatcctta tcaatcatta gatgtcaact taagcagttt ccaactctgg
     1321 atctaccctt tcttaaaagt ttgactttaa ctatgaacaa agggtctatc agttttaaaa
     1381 aagtggccct accaagtctc agctatctag atcttagtag aaatgcactg agctttagtg
     1441 gttgctgttc ttattctgat ttgggaacaa acagcctgag acacttagac ctcagcttca
     1501 atggtgccat cattatgagt gccaatttca tgggtctaga agagctgcag cacctggatt
     1561 ttcagcactc tactttaaaa agggtcacag aattctcagc gttcttatcc cttgaaaagc
     1621 tactttacct tgacatctct tatactaaca ccaaaattga cttcgatggt atatttcttg
     1681 gcttgaccag tctcaacaca ttaaaaatgg ctggcaattc tttcaaagac aacacccttt
     1741 caaatgtctt tgcaaacaca acaaacttga cattcctgga tctttctaaa tgtcaattgg
     1801 aacaaatatc ttggggggta tttgacaccc tccatagact tcaattatta aatatgagtc
     1861 acaacaatct attgtttttg gattcatccc attataacca gctgtattcc ctcagcactc
     1921 ttgattgcag tttcaatcgc atagagacat ctaaaggaat actgcaacat tttccaaaga
     1981 gtctagcctt cttcaatctt actaacaatt ctgttgcttg tatatgtgaa catcagaaat
     2041 tcctgcagtg ggtcaaggaa cagaagcagt tcttggtgaa tgttgaacaa atgacatgtg
     2101 caacacctgt agagatgaat acctccttag tgttggattt taataattct acctgttata
     2161 tgtacaagac aatcatcagt gtgtcagtgg tcagtgtgat tgtggtatcc actgtagcat
     2221 ttctgatata ccacttctat tttcacctga tacttattgc tggctgtaaa aagtacagca
     2281 gaggagaaag catctatgat gcatttgtga tctactcgag tcagaatgag gactgggtga
     2341 gaaatgagct ggtaaagaat ttagaagaag gagtgccccg ctttcacctc tgccttcact
     2401 acagagactt tattcctggt gtagccattg ctgccaacat catccaggaa ggcttccaca
     2461 agagccggaa ggttattgtg gtagtgtcta gacactttat tcagagccgt tggtgtatct
     2521 ttgaatatga gattgctcaa acatggcagt ttctgagcag ccgctctggc atcatcttca
     2581 ttgtccttga gaaggttgag aagtccctgc tgaggcagca ggtggaattg tatcgccttc
     2641 ttagcagaaa cacctacctg gaatgggagg acaatcctct ggggaggcac atcttctgga
     2701 gaagacttaa aaatgcccta ttggatggaa aagcctcgaa tcctgagcaa acagcagagg
     2761 aagaacaaga aacggcaact tggacctgag gagaacaaaa ctctggggcc taaacccagt
     2821 ctgtttgcaa ttaataaatg ctacagctca cctggggctc tgctatggac cgagagccca
     2881 tggaacacat ggctgctaag ctatagcatg gaccttaccg ggcagaagga agtagcactg
     2941 acaccttcct ttccaggggt atgaattacc taactcggga aaagaaacat aatccagaat
     3001 ctttaccttt aatctgaagg agaagaggct aaggcctagt gagaacagaa aggagaacca
     3061 gtcttcactg ggccttttga atacaagcca tgtcatgttc tgtgtttcag ttgctttaga
     3121 agagtattga tagtttcaac tgaactgaac ggtttcttac tttccctttt ttctactgaa
     3181 tgcaatatta aatagctctt tttgagaggt cttcattcca atttcatctt ccattttatg
     3241 tcattttctt ttcttttttt tttttatcta attctataag aaatatgatt gatacacgct
     3301 cacagatagc ctggccaatc ctaagaatgc tatatttatt aaatacaatt cctagtatac
     3361 ttttactttt ataaattcag ttatcgtttt tcatgccttg actataaact aatatcataa
     3421 ataagattgt tacaggtatg ctaagaaggc ccatatttga ctataatttt ttaagaaagt
     3481 atgtaaaata tactttgtca tattgtcact gaatgtcatt cttaagttat tacctaagtt
     3541 atggatgtca cagagtcagt gttaaaaata atttggttga tagaaatatt tttaatcagg
     3601 agggaaaagt ggagaggggt gcaggaacag aaatcatgat ttcatcattt attcttgatt
     3661 tttccggaag ttcacatagc tgaatgacaa gactacatat gctgcaactg atgttccttc
     3721 tcatcaagga tactctctga aggacttgag aacattttgg ggaggaagaa aggtctaaca
     3781 tccttttcct tcatcattct catttctgga catgccttgt gagatggatg aatgttggga
     3841 gtacacattt ctgctttcac cttatttcag tcagcatgaa cactgaatat ataatgtcat
     3901 ttcacagtgt gtgtgtgtgt gtgttgtgta tgtacatata tgaacctgta catgtgttta
     3961 agtttaaaga gaaaatagtg tacagagcag ctctatattt gtgatagggc tttaaatagt
     4021 tgagctaatt cagaaaagta tggagatttc ttggtaaagg aaaccaaagt agaatcatta
     4081 caagatctaa caataaaaat tttgaaacaa tcctacaagt aaatatattg gattttcttg
     4141 tccattaaga caatattcat actattgaaa ttatggaaac aacccttgga aggttaatgc
     4201 atagagacag aatgctatct acttgcagtg gaatgtgatt tgaccttgga gaagaagcaa
     4261 accttgctac ttgtgagcag atgcataaag gtggaggttt tttattgtaa gtgaaatatg
     4321 ccaggcacag aaggaactgg cctttcagga acttttgatg acatgagcaa agttagaaaa
     4381 aataatatgc agaacaatag aagaggaaga caaaagaaag acagccctag gatgtattct
     4441 tcacaacgat tttaaacaat atgcttgaaa gagaatgaag ttattagtat caattaagat
     4501 gtctacaatt ttcataattc cattcaaact ggaacatagc cacctaatta tttgtctctt
     4561 gttagccaag tgaaatagca gatcaagaat ctccccattt ttctgatata aaaacccaaa
     4621 ttctaatgca gtaaatgtct tgtcaatcag ccagatagca cagaagaggc aaggcgacag
     4681 tctgtgcccc ttccctctca cagaaactcc tgtgcactct agcccactgc ttcaggctac
     4741 aagctagaaa agcaagaagt gaaagtgcca cagttctcta tgtggttagt gccagtcagg
     4801 gtcattcaac ttaaaccatg agtcattaag aaaatacata tgcatgcatg cattaatgca
     4861 cagagtagtt tatttataac aactctttcc ataaagggct ggggagtttt caacaaaata
     4921 taaaggaaca attagtttaa tcaaaagaaa gaaatatagg cagaagaaag aaatgaaaga
     4981 aagaaaggaa agttttaact gtgtattcca ggtttaattc tagagatctt ctggaatttt
     5041 agagagtgtg acttttggag aattcctaaa ctcattttca gattatatta cgtatgtgac
     5101 ttggccttca tctgtctgag agctaagaaa gaaatgaaga tcatgcattt attattaggc
     5161 cattacaaac taataaatat aaagataaaa gggagactct gtggatgagt ctccctcttg
     5221 gctttcttat gggtagtcag agagaagcac tcagtagcct tatccttgac aacatttttg
     5281 tcacatttgt tttcccagtc tgtaggacaa cagcagtcct tatgactaaa gtagattgta
     5341 tcttttttac ctagcttcta ttcatctgtg ttgtcctagc ttcctttttg agtctacagc
     5401 ctttgagaaa tcactagaag tcactggaac ctcatgcttt gacttgaggc agtcctcata
     5461 tgtgttccta ggtactcgag gggtcagttg ggagactggg gagccatatc ttaaccatca
     5521 gctttgcttc cttggtgttg agcatcatgc ctgacaaagt aagcagacaa tgcctgtata
     5581 cgtgaagaag aggagaatca ttaatgcatg ttttcttggt gtgctgttgt ccttgataca
     5641 ttccagttca gaatctaaag tcctagggat cttagctgtc aacttagttt tccctgtctg
     5701 tcactttgta tggatgattt aaattgcttc ttcacttggt tgcttgacac catgtattct
     5761 aaaattttgt ggaaggtgtg tgttgggggg gggcgtagtt ctaacaatag tgttctctag
     5821 tggatacatt aaaatcatat tcagctaatt aatatttgat taagttttgc atgctatacc
     5881 gatttgataa acattcacaa aatcacaggc ttcaagattt ttcttaacac atccaaagta
     5941 cacaggcatt aaatgggcaa aactaaatat caaactgact ttatttaata gtttctctac
     6001 tgttctcttt tgttttatgt caagagttga atgccactgt tctgtatttt taattattta
     6061 ttgtttgcta ttgtgagaat tcaaagccag aactttgagg agctgacaga ggcactgtgg
     6121 cctatgaaga cagtttttgg agttaacaat ttccttggta actatggact atgtctccac
     6181 acttcagctc tcatatctga tggaataaac tcctttccag gaggcttcta cttatgctaa
     6241 tgcacccaag caaacaagga ggctaataga accagctgtt tctgtcttta tagcaatttc
     6301 ccaacattct acacttgagg atttcttctg tcacatgatt tttttcattg ggcattcttt
     6361 caatccttca ttaaatggcc gagacttctc actagacccc aactcaatga aattcttaag
     6421 ctgctagcat tgaacaacac tgactttttc aaagcacctt gatagggaat ttaagctgga
     6481 ccatctgaag caggaaagtc tgttgttttg atggaatttc ctaatggtac cattgtggct
     6541 ttattttgcc ttgttaatgt aagggattca aagcatttca acttactact catagttcaa
     6601 gcatctattt tgcagatgca ctgaaaatta agagattgga gagtttgtca tatatatttc
     6661 catcatcaac tattctagtt cttactaaag aaggagggtg caaaaatttg aaggatatgt
     6721 taaagtgcct tctatactta atgattcttc tagaaaaggc aaagtgttga tcttgttctt
     6781 tgttatggta ttatatcttc tcatggtaat ttgaaagaag tttacatacc aatttcagtt
     6841 tgtttaccta ggccttgaga gtcattctac agtacacgat taggctacta tgaagacaaa
     6901 agaaatcatt gtggggaaac tcagtacagc tctagattta ccttttataa tagatgaatc
     6961 ccagaatgat aaagatcaag cctggcatga tgttaattta gtgggctagg atcctggaaa
     7021 cctcctaaaa taggacatcc catgcatttg gccttagcca gtgaggcatc tctgagaaag
     7081 tgtagaaaaa cttgcaagga ggttcagtgc tctgaaagac acagagtcaa atgtacatgt
     7141 aattccagtt cttcttttat atatgtgtac tttacatagt ccctgaagta tcgagaggct
     7201 caggtatagg tgctaccacc ttgatagagt tcacttagcc aaaatgcaga aatggatgcc
     7261 cagagagaat agattacttg tcctgcatcc tgtaacttaa aatgtgttaa taatcatcat
     7321 aataaattct atctgccaaa tatttcatat gtgcatgaga ctgttttagt ttaattatta
     7381 aaattgcttt ctgatgcagc tcttagccac attgtcattt cccatacaat gaaactgaga
     7441 ccaaaaagca aattctccaa ttccaagggt agaattcaag taatcctgat atccagagct
     7501 gctaattttt tgccacacag tagactgctg cagtgtctgg gcttttttgc tggggctcat
     7561 tcactcacta acgggagaat cctgtggaca aggtcagcaa ctcccttacc atctagaaat
     7621 tgaaggtttc aaaggcactg catgtgactt tccttgattt ctatggaaat gaagatggtc
     7681 cctcctgtga cagtgctaag tgccgagtct gagtgtaaat gtgctttttg gcacaaattg
     7741 ttctgttcta atagtgttga ttataattat aaaataatgt gtttctgaaa ggctgcaagc
     7801 aattctggga atgacaataa gggtttcgaa acaacatggt atttatgtga gaagtgtttt
     7861 gttgaaaatt aaacctgtgt ttaggagaaa g
//
