LOCUS       NM_001112705            5481 bp    mRNA    linear   ROD 04-OCT-2024
DEFINITION  Mus musculus tousled-like kinase 2 (Arabidopsis) (Tlk2), transcript
            variant A, mRNA.
ACCESSION   NM_001112705
VERSION     NM_001112705.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 5481)
  AUTHORS   Lin,H.Y., Mohammadhosseini,M., McClatchy,J., Villamor-Paya,M.,
            Jeng,S., Bottomly,D., Tsai,C.F., Posso,C., Jacobson,J., Adey,A.,
            Gosline,S., Liu,T., McWeeney,S., Stracker,T.H. and Agarwal,A.
  TITLE     The TLK-ASF1 histone chaperone pathway plays a critical role in
            IL-1beta-mediated AML progression
  JOURNAL   Blood 143 (26), 2749-2762 (2024)
   PUBMED   38498025
REFERENCE   2  (bases 1 to 5481)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   3  (bases 1 to 5481)
  AUTHORS   Correia,S.P., Chan,A.B., Vaughan,M., Zolboot,N., Perea,V.,
            Huber,A.L., Kriebs,A., Moresco,J.J., Yates,J.R. 3rd and Lamia,K.A.
  TITLE     The circadian E3 ligase complex SCFFBXL3+CRY targets TLK2
  JOURNAL   Sci Rep 9 (1), 198 (2019)
   PUBMED   30655559
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 5481)
  AUTHORS   Segura-Bayona,S., Knobel,P.A., Gonzalez-Buron,H., Youssef,S.A.,
            Pena-Blanco,A., Coyaud,E., Lopez-Rovira,T., Rein,K., Palenzuela,L.,
            Colombelli,J., Forrow,S., Raught,B., Groth,A., de Bruin,A. and
            Stracker,T.H.
  TITLE     Differential requirements for Tousled-like kinases 1 and 2 in
            mammalian development
  JOURNAL   Cell Death Differ 24 (11), 1872-1885 (2017)
   PUBMED   28708136
  REMARK    GeneRIF: TLK1 and TLK2 have largely redundant roles in genome
            maintenance.
REFERENCE   5  (bases 1 to 5481)
  AUTHORS   Liu,H., Dowdle,J.A., Khurshid,S., Sullivan,N.J., Bertos,N.,
            Rambani,K., Mair,M., Daniel,P., Wheeler,E., Tang,X., Toth,K.,
            Lause,M., Harrigan,M.E., Eiring,K., Sullivan,C., Sullivan,M.J.,
            Chang,S.W., Srivastava,S., Conway,J.S., Kladney,R., McElroy,J.,
            Bae,S., Lu,Y., Tofigh,A., Saleh,S.M.I., Fernandez,S.A.,
            Parvin,J.D., Coppola,V., Macrae,E.R., Majumder,S., Shapiro,C.L.,
            Yee,L.D., Ramaswamy,B., Hallett,M., Ostrowski,M.C., Park,M.,
            Chamberlin,H.M. and Leone,G.
  TITLE     Discovery of Stromal Regulatory Networks that Suppress
            Ras-Sensitized Epithelial Cell Proliferation
  JOURNAL   Dev Cell 41 (4), 392-407 (2017)
   PUBMED   28535374
REFERENCE   6  (bases 1 to 5481)
  AUTHORS   Zhang,J., Gao,G., Begum,G., Wang,J., Khanna,A.R., Shmukler,B.E.,
            Daubner,G.M., de Los Heros,P., Davies,P., Varghese,J.,
            Bhuiyan,M.I., Duan,J., Zhang,J., Duran,D., Alper,S.L., Sun,D.,
            Elledge,S.J., Alessi,D.R. and Kahle,K.T.
  TITLE     Functional kinomics establishes a critical node of volume-sensitive
            cation-Cl- cotransporter regulation in the mammalian brain
  JOURNAL   Sci Rep 6, 35986 (2016)
   PUBMED   27782176
  REMARK    Publication Status: Online-Only
REFERENCE   7  (bases 1 to 5481)
  AUTHORS   Piao,Y., Ko,N.T., Lim,M.K. and Ko,M.S.
  TITLE     Construction of long-transcript enriched cDNA libraries from
            submicrogram amounts of total RNAs by a universal PCR amplification
            method
  JOURNAL   Genome Res 11 (9), 1553-1558 (2001)
   PUBMED   11544199
REFERENCE   8  (bases 1 to 5481)
  AUTHORS   Sillje,H.H., Takahashi,K., Tanaka,K., Van Houwe,G. and Nigg,E.A.
  TITLE     Mammalian homologues of the plant Tousled gene code for
            cell-cycle-regulated kinases with maximal activities linked to
            ongoing DNA replication
  JOURNAL   EMBO J 18 (20), 5691-5702 (1999)
   PUBMED   10523312
REFERENCE   9  (bases 1 to 5481)
  AUTHORS   Zhang,S., Xing,H. and Muslin,A.J.
  TITLE     Nuclear localization of protein kinase U-alpha is regulated by
            14-3-3
  JOURNAL   J Biol Chem 274 (35), 24865-24872 (1999)
   PUBMED   10455159
REFERENCE   10 (bases 1 to 5481)
  AUTHORS   Shalom,S. and Don,J.
  TITLE     Tlk, a novel evolutionarily conserved murine serine threonine
            kinase, encodes multiple testis transcripts
  JOURNAL   Mol Reprod Dev 52 (4), 392-405 (1999)
   PUBMED   10092119
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL645471.8.
            
            On Jul 1, 2014 this sequence version replaced NM_001112705.1.
            
            Transcript Variant: This variant (A) encodes the longest isoform
            (A).
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK165667.1, SRR1660819.71605.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849380, SAMN00849381
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-525               AL645471.8         24934-25458
            526-608             AL645471.8         27619-27701
            609-680             AL645471.8         50787-50858
            681-750             AL645471.8         52259-52328
            751-794             AL645471.8         53201-53244
            795-890             AL645471.8         53952-54047
            891-1058            AL645471.8         64593-64760
            1059-1154           AL645471.8         83768-83863
            1155-1247           AL645471.8         84976-85068
            1248-1358           AL645471.8         90181-90291
            1359-1495           AL645471.8         90866-91002
            1496-1648           AL645471.8         92892-93044
            1649-1715           AL645471.8         96698-96764
            1716-1813           AL645471.8         98373-98470
            1814-1895           AL645471.8         100277-100358
            1896-1987           AL645471.8         103668-103759
            1988-2077           AL645471.8         110560-110649
            2078-2247           AL645471.8         113033-113202
            2248-2386           AL645471.8         114506-114644
            2387-2498           AL645471.8         119244-119355
            2499-2606           AL645471.8         122525-122632
            2607-5481           AL645471.8         124492-127366
FEATURES             Location/Qualifiers
     source          1..5481
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="11"
                     /map="11 68.61 cM"
     gene            1..5481
                     /gene="Tlk2"
                     /gene_synonym="4933403M19Rik; PKU-alpha; PKUalpha; Tlk"
                     /note="tousled-like kinase 2 (Arabidopsis)"
                     /db_xref="GeneID:24086"
                     /db_xref="MGI:MGI:1346023"
     exon            1..525
                     /gene="Tlk2"
                     /gene_synonym="4933403M19Rik; PKU-alpha; PKUalpha; Tlk"
                     /inference="alignment:Splign:2.1.0"
     exon            526..608
                     /gene="Tlk2"
                     /gene_synonym="4933403M19Rik; PKU-alpha; PKUalpha; Tlk"
                     /inference="alignment:Splign:2.1.0"
     CDS             528..2780
                     /gene="Tlk2"
                     /gene_synonym="4933403M19Rik; PKU-alpha; PKUalpha; Tlk"
                     /EC_number="2.7.11.1"
                     /note="isoform A is encoded by transcript variant A;
                     serine/threonine-protein kinase tousled-like 2; protein
                     kinase U-alpha"
                     /codon_start=1
                     /product="serine/threonine-protein kinase tousled-like 2
                     isoform A"
                     /protein_id="NP_001106176.1"
                     /db_xref="CCDS:CCDS48954.1"
                     /db_xref="GeneID:24086"
                     /db_xref="MGI:MGI:1346023"
                     /translation="MMEELHSLDPRRQELLEARFTGVGVSKGPLNSESSNQSLCSVGS
                     LSDKEVETPEKKQNDQRNRKRKAEPYDTSQGKGTPRGHKISDYFEFAGGSGPGTSPGR
                     SVPPVARSSPQHSLSNPLPRRAEQPLYGLDGSAAKEASEEQSALPTLMSVMLAKPRLD
                     TEQLAPRGAGLCFTFVSAQQNSPSSTGSGNTEHSCSSQKQISIQHRQTQSDLTIEKIS
                     ALENSKNSDLEKKEGRIDDLLRANCDLRRQIDEQQKMLEKYKERLNRCVTMSKKLLIE
                     KSKQEKMACRDKSMQDRLRLGHFTTVRHGASFTEQWTDGYAFQNLIKQQERINSQREE
                     IERQRKMLAKRKPPAMGQAPPATNEQKQRKSKTNGAENETLTLAEYHEQEEIFKLRLG
                     HLKKEEAEIQAELERLERVRNLHIRELKRIHNEDNSQFKDHPTLNDRYLLLHLLGRGG
                     FSEVYKAFDLTEQRYVAVKIHQLNKNWRDEKKENYHKHACREYRIHKELDHPRIVKLY
                     DYFSLDTDSFCTVLEYCEGNDLDFYLKQHKLMSEKEARSIIMQIVNALKYLNEIKPPI
                     IHYDLKPGNILLVNGTACGEIKITDFGLSKIMDDDSYNSVDGMELTSQGAGTYWYLPP
                     ECFVVGKEPPKISNKVDVWSVGVIFYQCLYGRKPFGHNQSQQDILQENTILKATEVQF
                     PPKPVVTPEAKAFIRRCLAYRKEDRIDVQQLACDPYLLPHIRKSVSTSSPAGAAIAST
                     SGASNNSSSN"
     exon            609..680
                     /gene="Tlk2"
                     /gene_synonym="4933403M19Rik; PKU-alpha; PKUalpha; Tlk"
                     /inference="alignment:Splign:2.1.0"
     exon            681..750
                     /gene="Tlk2"
                     /gene_synonym="4933403M19Rik; PKU-alpha; PKUalpha; Tlk"
                     /inference="alignment:Splign:2.1.0"
     exon            751..794
                     /gene="Tlk2"
                     /gene_synonym="4933403M19Rik; PKU-alpha; PKUalpha; Tlk"
                     /inference="alignment:Splign:2.1.0"
     exon            795..890
                     /gene="Tlk2"
                     /gene_synonym="4933403M19Rik; PKU-alpha; PKUalpha; Tlk"
                     /inference="alignment:Splign:2.1.0"
     exon            891..1058
                     /gene="Tlk2"
                     /gene_synonym="4933403M19Rik; PKU-alpha; PKUalpha; Tlk"
                     /inference="alignment:Splign:2.1.0"
     exon            1059..1154
                     /gene="Tlk2"
                     /gene_synonym="4933403M19Rik; PKU-alpha; PKUalpha; Tlk"
                     /inference="alignment:Splign:2.1.0"
     exon            1155..1247
                     /gene="Tlk2"
                     /gene_synonym="4933403M19Rik; PKU-alpha; PKUalpha; Tlk"
                     /inference="alignment:Splign:2.1.0"
     exon            1248..1358
                     /gene="Tlk2"
                     /gene_synonym="4933403M19Rik; PKU-alpha; PKUalpha; Tlk"
                     /inference="alignment:Splign:2.1.0"
     exon            1359..1495
                     /gene="Tlk2"
                     /gene_synonym="4933403M19Rik; PKU-alpha; PKUalpha; Tlk"
                     /inference="alignment:Splign:2.1.0"
     exon            1496..1648
                     /gene="Tlk2"
                     /gene_synonym="4933403M19Rik; PKU-alpha; PKUalpha; Tlk"
                     /inference="alignment:Splign:2.1.0"
     exon            1649..1715
                     /gene="Tlk2"
                     /gene_synonym="4933403M19Rik; PKU-alpha; PKUalpha; Tlk"
                     /inference="alignment:Splign:2.1.0"
     exon            1716..1813
                     /gene="Tlk2"
                     /gene_synonym="4933403M19Rik; PKU-alpha; PKUalpha; Tlk"
                     /inference="alignment:Splign:2.1.0"
     exon            1814..1895
                     /gene="Tlk2"
                     /gene_synonym="4933403M19Rik; PKU-alpha; PKUalpha; Tlk"
                     /inference="alignment:Splign:2.1.0"
     exon            1896..1987
                     /gene="Tlk2"
                     /gene_synonym="4933403M19Rik; PKU-alpha; PKUalpha; Tlk"
                     /inference="alignment:Splign:2.1.0"
     exon            1988..2077
                     /gene="Tlk2"
                     /gene_synonym="4933403M19Rik; PKU-alpha; PKUalpha; Tlk"
                     /inference="alignment:Splign:2.1.0"
     exon            2078..2247
                     /gene="Tlk2"
                     /gene_synonym="4933403M19Rik; PKU-alpha; PKUalpha; Tlk"
                     /inference="alignment:Splign:2.1.0"
     exon            2248..2386
                     /gene="Tlk2"
                     /gene_synonym="4933403M19Rik; PKU-alpha; PKUalpha; Tlk"
                     /inference="alignment:Splign:2.1.0"
     exon            2387..2498
                     /gene="Tlk2"
                     /gene_synonym="4933403M19Rik; PKU-alpha; PKUalpha; Tlk"
                     /inference="alignment:Splign:2.1.0"
     exon            2499..2606
                     /gene="Tlk2"
                     /gene_synonym="4933403M19Rik; PKU-alpha; PKUalpha; Tlk"
                     /inference="alignment:Splign:2.1.0"
     exon            2607..5481
                     /gene="Tlk2"
                     /gene_synonym="4933403M19Rik; PKU-alpha; PKUalpha; Tlk"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3676..3681
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Tlk2"
                     /gene_synonym="4933403M19Rik; PKU-alpha; PKUalpha; Tlk"
                     /note="hexamer: AATAAA"
     polyA_site      3699
                     /gene="Tlk2"
                     /gene_synonym="4933403M19Rik; PKU-alpha; PKUalpha; Tlk"
                     /note="major polyA site"
     regulatory      3819..3824
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Tlk2"
                     /gene_synonym="4933403M19Rik; PKU-alpha; PKUalpha; Tlk"
                     /note="hexamer: ATTAAA"
     polyA_site      3837
                     /gene="Tlk2"
                     /gene_synonym="4933403M19Rik; PKU-alpha; PKUalpha; Tlk"
     regulatory      5455..5460
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Tlk2"
                     /gene_synonym="4933403M19Rik; PKU-alpha; PKUalpha; Tlk"
                     /note="hexamer: AATAAA"
     polyA_site      5481
                     /gene="Tlk2"
                     /gene_synonym="4933403M19Rik; PKU-alpha; PKUalpha; Tlk"
ORIGIN      
        1 agccgtgcaa atctcgcgag gaaacacgcg gtttcactag tgtgtttaca ccgatcacta
       61 ctaatccgga ccgaaccgat ccggattaag gggccggagg cgggtcctgg gcactagcgg
      121 ttccgacccc ccccccatcc cgccctccgc gccgcacccg agtggccccc agccgagcgg
      181 gcccccactt ctcggcccga cccagaccct tctgcgcctc ccttggcctt tgtccggcgc
      241 caggaggccg gccccgcgcc ccccggcggc cgcccgggcc cgggcccgcg tcagccgcct
      301 ggctccgtac gcgagctcgg ggatctgcgg cctccgtggc ccccctcccc cgcccgccct
      361 gtcgtggagc ccggcgccgc cggcggctgc ccgggcgggg ggttgcggcg ctcaggagag
      421 gccccggctc cgccccgggc ctgcccaggg ggagagcgga gcggtcagca gccgggtcgg
      481 gtcggggccc ctcccgagag gagagtggag cggcagcggc ggcagaaatg atggaagaac
      541 tgcatagcct ggacccacga aggcaggaat tactagaggc caggttcact ggagttggtg
      601 taagtaaggg gccactcaac agtgagtctt ccaaccagag tctgtgcagc gtggggtcgt
      661 tgagtgataa agaagtagag actcctgaga aaaagcagaa tgaccagcga aatcggaaaa
      721 ggaaagccga gccatatgac actagccaag ggaaaggcac tcctagggga cataaaatta
      781 gtgattactt tgagtttgct gggggaagcg ggccaggaac cagccctggc agaagtgttc
      841 caccagttgc acgatcctca ccgcaacatt ccttatccaa tcccttaccg cgtcgagcag
      901 aacagcctct gtatggttta gatggcagtg cagcaaagga ggcctcagaa gagcagtctg
      961 ctctgcccac cctcatgtca gtgatgttag caaaaccgcg acttgacaca gagcagttag
     1021 cgccacgggg agctggcctc tgcttcactt tcgtctctgc tcaacaaaac agtccttctt
     1081 ctacgggttc tggcaataca gaacattctt gcagctccca aaaacagatc tccatccagc
     1141 acagacagac ccagtctgac ctcacaatag aaaaaatatc tgcactagaa aacagtaaga
     1201 actctgactt agagaagaag gaaggaagaa tagatgattt attaagagcc aactgtgatt
     1261 tgagacgaca gatagatgaa cagcaaaaga tgctagagaa atacaaggaa cgattaaata
     1321 gatgtgtcac catgagcaag aagctcctta tagaaaagtc aaaacaagag aagatggcgt
     1381 gcagagataa gagcatgcag gaccgattgc gattaggcca ctttactact gtccggcatg
     1441 gagcctcgtt tactgagcag tggacagatg gttatgcttt ccaaaacctc atcaagcaac
     1501 aggaaaggat aaattcacag agagaagaga tagaaaggca acggaaaatg ttagcaaaac
     1561 ggaaacctcc tgccatgggt caggcccctc ctgcaaccaa tgagcagaaa caacggaaaa
     1621 gcaagactaa tggagctgaa aatgaaacgt taacgttagc cgagtaccat gaacaagagg
     1681 aaatcttcaa acttagatta ggtcatctta agaaagagga agcagaaatc caggcagagc
     1741 tggaaaggct ggaaagggtt aggaatctac acatcaggga attaaaaagg atacataatg
     1801 aagacaattc gcagtttaaa gaccatccaa cactaaatga cagatatttg ttgttacatc
     1861 ttttgggtag aggaggtttc agtgaagttt acaaggcatt tgatctaacg gagcaaagat
     1921 atgtagctgt gaaaattcac cagttaaata aaaactggag agatgagaaa aaggagaatt
     1981 accacaagca tgcgtgtagg gaataccgga ttcacaagga gctggaccac cccaggatag
     2041 tgaagctgta tgattacttt tcactggaca ctgactcgtt ttgtacagta ttagaatact
     2101 gtgaagggaa tgacctggac ttctacctaa aacagcacaa attaatgtcg gagaaagaag
     2161 cccgatccat tattatgcag attgtgaatg ctttaaagta cttaaatgaa ataaaacctc
     2221 ccatcataca ctatgacctc aaaccaggta atatcctttt agtaaatggt acagcatgtg
     2281 gagagataaa aattacagat tttggtcttt ccaagatcat ggatgatgat agctacaatt
     2341 cagtggatgg catggagctg acgtcacaag gagctggtac ttattggtat ttaccaccag
     2401 agtgttttgt ggttgggaaa gagccaccaa agatctcaaa taaagtcgat gtttggtcag
     2461 tgggtgtgat cttctaccag tgtctttatg ggaggaagcc ttttggccat aaccagtccc
     2521 agcaagatat tctacaagag aatactattc ttaaggctac tgaagtacag ttcccgccaa
     2581 agccagtagt aacacctgaa gcaaaggcat ttatcaggag atgtctggcc tatcgaaagg
     2641 aagatcgcat tgatgtgcag cagctggcct gtgaccccta cttgttgcct cacatccgaa
     2701 agtcagtctc cacaagtagc cctgcaggag ctgctattgc atcaacctct ggggcatcca
     2761 ataacagttc ttcgaattga gaccaactcc taaggccaca aactgttgag cacacacagt
     2821 ggacatacgg cattcagcag tgggtttgga acatagtgaa tccgaatgga tctcatgaaa
     2881 cctgtaccag gtgcttttat tttcttgctt tttttcccca tccatagagc atgacagcat
     2941 cgattctcac tgaggagaag ccttgggcag ctctggccag gcctcatagg aaaaggtccc
     3001 gcccaaagtt ccagcgtcaa cggtcaccat gggtggctgc tctgagtgag gaaaaaaatt
     3061 aaaaagagaa aactggttcc atgtactgtg aacttggaaa catgcagact cagggggttc
     3121 cctgacggag tgcttcagag gaagaatgtg gacttgaaaa tacagactgg gctagtccag
     3181 tgtctatatt taaacttgct cttttctttt aataaaattt aggtaacatc tcctgaaaag
     3241 ctggtagcac aaggctcggc tggggatggt gtttgacttg ggaggaaaaa gttgctactg
     3301 ccattaaagg cactagagtt agtgtgctag ccctgaataa tctcagtttc taaaaacatg
     3361 cagcttcctt cacccctttt ttatttttga aagaatacat ttggtcataa agtgaaaccc
     3421 gtattagcga gtacgtggca atgttcattc cggtcagatg cagctttctc ctccatctgg
     3481 tctcccgcgt gaatgcttcc ctcgcctcag tagggagaaa cagtgggagt tctgagatgc
     3541 ctgggttttg ccatcggaca aagaatgagg ttagaagact gcagtttgga gtctctctgg
     3601 gttttcagat acttcttcac aatttgaaca cttgacggtt gtccctttta atttatttga
     3661 agtgctattt ttttaaataa aggttcatct gtccatgcag tcctcctgga ttctctgatt
     3721 atatatagta actatgggaa ctgcttccag aagtagacag acggctcgaa gggtgtctag
     3781 ctcctgccca caatcttctt gctcatttct tgctaaccat taaactcgat caacacatgg
     3841 gcttttccaa ctctgttaga tctttgagga gaacaaatcc tctctcaggc atttgaattg
     3901 tggtgaatat gctctggtgt gtgtgtgagc atggaccctc ttcttactca cttagagttt
     3961 gtttttttta aatctcaagg aatataggta tttcagcttt tcttcccccc ccccccccca
     4021 atttggctta tttctgagat gtgtgatctc ctggaggtgg agctgaaatg ggagccgtgt
     4081 gaccccactg tgccctggaa acagcagatg actctgcagg agagagttgg agccagggcc
     4141 tgaacatgtg gtagccagct tggaatgtta ctgacatgtg gtaagagagc cagccagaaa
     4201 tacttagagc tggagagatc agagcagggg cgggagtcag gaagggcaga gctcttaagg
     4261 caattcatgc ctctagtgct ctgtgcttgc taaaagtgtt cctaaggaca ccttttctaa
     4321 acccctgtgc attttgtagt cctgccccac actccttggt atcaagtgat cttaaagaaa
     4381 ggcagagcat tccaaaccaa aggaacttgt aggtaggttt tgtcgtatgg tgctctgagg
     4441 agagaaaact cctgaggctt tggtgaaatg tgtattttgt gtgtacctct cccctgctta
     4501 aatgctaggg ctggagcagg accttccgta tgccaggcaa gtgcagctct agcctcaacc
     4561 tgcgctccca tcctgctcac tgcctgtagc ttcagtgatg ataggatgtg tccgtccgca
     4621 ggcacgagga gagggtgaag tagaagccat ggtaaactag cgcaaaggaa ctcaatgtgc
     4681 gaatgagtga ttcttgcaat ttttaagtct tgactcagtg tggagaaaga tgctgtggct
     4741 ttaggccctc cttccctcac ttttggccca tttcctatgt ccttaggagt gtgtggcagg
     4801 cttactttgt gtctgccttt tctcccttta tcctaaagaa gggcgccatc ccttctcatg
     4861 tgaatcagtc tgagtcttgc ctggtctggc ttgaatctgc gcacggcatc tttccactgc
     4921 ctggagaagc cccagagaac atacactggc ctcttttcct ctttgaagga gccagtaaaa
     4981 tcattgagtg ggtttctctg atgtcagatg agtttcactt tctccaaggt gtagtccctt
     5041 attgccacag tgttgactta ctctgtccag tgcatccggc atccttttaa cattgaggtg
     5101 tgactgtcat ctatagatat gctagattgc attcctgact gttctgggac acatgtggcc
     5161 catggctgga ctcttcctgt aatacagtga ccaaagaaag tattaattta taattaaact
     5221 tatccatact gttttggcct tttccaacat tggatatagg gcacaaggac atacatttaa
     5281 aataaattat aagaaattag ggtgatgaga tggcttagca agtaaaggaa cttgctgaca
     5341 agcctgatta cctaaattta atccccgtga tcccagaaaa gtaacgccta caaattgtcc
     5401 tctgacctcc acatacctgc cttggtacta ccattaccca cacataaaca aattaataaa
     5461 tgtaatttta aaagaaacta a
//
