LOCUS       NM_001271574            6368 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus transketolase-like 2 (Tktl2), transcript variant 2,
            mRNA.
ACCESSION   NM_001271574
VERSION     NM_001271574.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 6368)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 6368)
  AUTHORS   Lu,Y., Oura,S., Matsumura,T., Oji,A., Sakurai,N., Fujihara,Y.,
            Shimada,K., Miyata,H., Tobita,T., Noda,T., Castaneda,J.M.,
            Kiyozumi,D., Zhang,Q., Larasati,T., Young,S.A.M., Kodani,M.,
            Huddleston,C.A., Robertson,M.J., Coarfa,C., Isotani,A.,
            Aitken,R.J., Okabe,M., Matzuk,M.M., Garcia,T.X. and Ikawa,M.
  TITLE     CRISPR/Cas9-mediated genome editing reveals 30 testis-enriched
            genes dispensable for male fertility in micedagger
  JOURNAL   Biol Reprod 101 (2), 501-511 (2019)
   PUBMED   31201419
REFERENCE   3  (bases 1 to 6368)
  AUTHORS   Bulusu,V., Prior,N., Snaebjornsson,M.T., Kuehne,A., Sonnen,K.F.,
            Kress,J., Stein,F., Schultz,C., Sauer,U. and Aulehla,A.
  TITLE     Spatiotemporal Analysis of a Glycolytic Activity Gradient Linked to
            Mouse Embryo Mesoderm Development
  JOURNAL   Dev Cell 40 (4), 331-341 (2017)
   PUBMED   28245920
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BY090827.1, AK029871.1, AC122800.3 and AV271587.2.
            
            Transcript Variant: This variant (2) contains an alternate exon
            structure in the 3' UTR, compared to variant 1. Variants 1 and 2
            encode the same protein.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK029871.1, SRR5189685.148372.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849384 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-19                BY090827.1         1-19
            20-934              AK029871.1         2-916
            935-5504            AK029871.1         918-5487
            5505-5806           AC122800.3         161876-162177
            5807-6368           AV271587.2         56-617
FEATURES             Location/Qualifiers
     source          1..6368
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="8"
                     /map="8 33.14 cM"
     gene            1..6368
                     /gene="Tktl2"
                     /gene_synonym="4933401I19Rik"
                     /note="transketolase-like 2"
                     /db_xref="GeneID:74419"
                     /db_xref="MGI:MGI:1921669"
     exon            1..1961
                     /gene="Tktl2"
                     /gene_synonym="4933401I19Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             53..1936
                     /gene="Tktl2"
                     /gene_synonym="4933401I19Rik"
                     /EC_number="2.2.1.1"
                     /codon_start=1
                     /product="transketolase-like protein 2"
                     /protein_id="NP_001258503.1"
                     /db_xref="CCDS:CCDS22334.1"
                     /db_xref="GeneID:74419"
                     /db_xref="MGI:MGI:1921669"
                     /translation="MALARDAKLESDTFQVLQDVANRLRIHSIRATCACSSGHPTSCC
                     SVAEIMAVLFFHTMRYKQADPEHPDNDRFVLSKGHAAPILYAVWVEVGRICESDLLNL
                     RKIHCDLEGHPTPRLSFVDVATGSLGQGLGAACGMAYTGKYFDKASYRVFCLMGDGES
                     SEGSVWEALAFASHYNLDNLVAIFDVNRLGQSGTAPLEHCTAVYEKRCQAFGWNTYVV
                     DGHDVEALCQAFWKAAQVKNKPTALIAKTFKGRGIPNVEDAENWHGKPMPKDRADGIV
                     KLIENRIQTNRNLTPKPPIEDSPRISMSNTKMTSLPVYKLGDMIATREAYGLALAKLG
                     QSNQRVIVLDGDTKNSTFSEVFKKEHPERFIECFIAEQNMVSVALGCATRGRTIAFVS
                     TFAAFLTRAFDQIRMGAISQTNINFVGSHCGVSVGEDGPSQMALEDLAMFRSIPNCTV
                     FYPSDAVSTEHAVYLAANTKGMCFIRTTRPKTAVIYTAEENFVIGQAKVIRQSAVDKV
                     TVIGAGVTLHEALVAAEELSQQGIFIRVIDLFTIKPLDAVTIIQSAKATGGQIITVED
                     HYREGGIGEAVCAAISREPDIVVRQLAVTEVPRSGKPSELLDMFGISARHIIAAVKDT
                     VMK"
     exon            1962..6368
                     /gene="Tktl2"
                     /gene_synonym="4933401I19Rik"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2792..2797
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Tktl2"
                     /gene_synonym="4933401I19Rik"
                     /note="hexamer: ATTAAA"
     polyA_site      2813
                     /gene="Tktl2"
                     /gene_synonym="4933401I19Rik"
ORIGIN      
        1 tgaggaagct gagcttcttg ctcttcgtac ggcagccgag ctgcctgcag ccatggcctt
       61 ggccagagat gccaagctgg agtcggacac ctttcaggtg cttcaggacg tggccaatcg
      121 cctgcgaatc cactcaatca gggccacgtg tgcctgcagc tccggacatc ccacgtcgtg
      181 ctgtagtgtg gcagagatca tggctgtgct cttcttccat accatgaggt acaaacaggc
      241 agacccagaa cacccggaca atgaccgctt tgtcctctcc aagggccacg ccgcgcccat
      301 cctctatgcg gtttgggtgg aggtgggccg tatctgtgaa tcggacttgc tgaacttgcg
      361 aaaaattcac tgcgacctgg agggtcatcc caccccacga ctgtcgtttg ttgatgtggc
      421 aacagggtcc cttgggcaag gactgggtgc tgcctgtgga atggcttaca ccggcaaata
      481 ctttgacaag gccagctacc gcgtgttctg cctcatgggg gatggtgagt cctcggaagg
      541 ttctgtctgg gaggccttgg cctttgcttc ccactacaac ttggacaatc tcgtggcaat
      601 ctttgacgtg aaccgcttgg gacaaagcgg cacagcaccc ctagagcact gtacagccgt
      661 ttatgagaag cgctgccaag cgtttgggtg gaatacttac gtggtggatg gtcatgatgt
      721 tgaagctctc tgccaggcct tttggaaagc agctcaagtc aagaataagc ctactgccct
      781 gattgccaag accttcaagg gtagaggtat tccaaatgtc gaggatgcgg aaaattggca
      841 tggaaagcct atgccaaaag acagagctga tgggattgtc aaattaattg agaatcggat
      901 acagacaaac cgaaatctca caccgaaacc ccccattgaa gactcgccca ggatcagcat
      961 gtccaataca aaaatgacct ctctgcctgt ctacaaactg ggagacatga tagctactcg
     1021 tgaagcatac ggcttggctc tggctaaatt gggccaatct aaccaaagag ttatcgttct
     1081 agatggcgac acaaaaaact ctaccttttc agaagtattc aagaaagagc accctgagcg
     1141 tttcatagag tgctttatcg ctgagcaaaa catggtaagt gtggccctgg ggtgtgccac
     1201 acgaggacga actattgctt tcgttagtac ctttgctgcc ttcctgaccc gagcctttga
     1261 tcagatccga atgggagcca tctctcaaac caacatcaac ttcgttggtt cccactgcgg
     1321 ggtgtctgtt ggagaagacg gaccttccca gatggccctg gaggacctag ccatgttcag
     1381 aagcatcccc aactgcactg ttttctatcc aagtgatgct gtctcgacag aacacgctgt
     1441 ttatctggca gccaacacga agggaatgtg cttcattcgt accactcggc caaaaacagc
     1501 agttatttat acagctgaag aaaactttgt gattggacag gccaaggtga tccgccagag
     1561 tgcagtcgac aaggtgacag ttattggagc aggggttact ctgcatgaag ccttagtagc
     1621 tgctgaggag ctttctcaac aagggatttt tattcgtgtc attgacctgt tcactatcaa
     1681 acctctggat gctgtcacca ttatccaaag tgccaaagcc acaggtggcc agatcatcac
     1741 tgtggaagat cactaccgag aaggtggcat tggtgaagct gtgtgtgcag ccatctctag
     1801 agagcctgac atagtcgttc gtcaacttgc agtaacagaa gtacctcgaa gcgggaaacc
     1861 tagcgagcta ctggatatgt ttggaatcag tgccagacac attatagcag ctgtgaaaga
     1921 taccgtaatg aaatgaaatt gcccaggctt catacatcaa ggcctgagca gttggttttc
     1981 cagcagggtg gagatatgac aggataaaac accaacctga gagttggaaa cacggagtcc
     2041 tggtgtttct gcactgcccc acactctaat ctctactttc aagataacca ctcctctttg
     2101 catgacattc ttaaataatt tagaaaaaaa tagttgatat aagaacgttg taaatattag
     2161 aatgggtaga tatggagaac atttgtcacc tcgatttctt gcctgcagtg atctgtagct
     2221 atgggcctgc taatagtccc acctgaacct agtaacatca gatgtcacat ttccccatga
     2281 gtggggtgct attgcaggat taagtctctt ccttatgctt tcttgcttca tggggataca
     2341 tggttgattt acaaactgtg aactagacaa aaatatcaaa acaaaagttc attggtttct
     2401 tcagctttta ttatttggaa atgtgggagg tctattttcc caaatatgca aaaacatgtg
     2461 tttaatagtg gcagtacatc tgaaaattta aatgtctaat ggaatcttgg agactgctag
     2521 ataatgcttt ataaaatgag attgggggtg agatttacca tatgctcagc atttcttaag
     2581 tgagagagag tgtgtgtctt gttcagtttt gctacacaat gagtgctctg aagaattgag
     2641 tccgtcctga ctagtgtaac cactcactgt taaaattgaa gacactaagg cttttcactt
     2701 ctgtttttgt tgctgttgtt gttattgttc ataaaatgat caaaacattt tctctgtttt
     2761 tcatgaaatc cactttaaca acttttaatg gattaaatta ttcttcagac agatcttgct
     2821 gtggtagctt gggatggcct caaacttgcg ttcatccact ggcttctatc tccccagtac
     2881 cgacatgaaa ggagtatgta tcatgccctg ttgaagatac agttttgtta agcaacaaaa
     2941 catggcagtg atttagtgtt gatattctgt tcaaggttgt gcctagaagc tcttttcgat
     3001 atcaggagag cttagtgata gttcagggat ggctgtcccc tcacaattgc cttgcttgtg
     3061 attgcagtag aaaggccagt gtgaaaatga cactcattcc agtcaacgta tgacttcttg
     3121 agttgtgtgg gtagtaaaag aataagggtg aatgttttag tttgccttaa gtacattgaa
     3181 aaggttattt aaataaaagt tcaattgagg catctatttg ggaaaaagag ccttttcaag
     3241 atagggcaaa agacatggca tttatgtcaa gataccagtg ctggtcctgg ttcctagggc
     3301 ctgtagtcct ggctaacttg gaaagcatat ataaaacact taaaaaataa acttacatgc
     3361 cataagtttc gatgaaaaag acaagcacat atgtatttta aaggaaattg cgccaatacc
     3421 taatactatg tccttatttc atatgattag aatagagcag aacttttgaa gtatgtattt
     3481 tattatcctc acctttacat gggataatga gatttaccct tagaaaatgg aacttgtcta
     3541 tttgcttcaa aacttatttc aagattctct ttgttcactt atattcgaca tttccatgtt
     3601 ttcctattta aagtgggtaa atttaaactc attactgttc agcacaagtt ttaaaaatct
     3661 agtgtaaata cccaggaaag aaaaattgta gcaatacatt aacagtgtgg cctcattaac
     3721 aaagtggcca gactctgaaa gaataaaagc acatgggaac tgtcagaaaa gcacacaggc
     3781 caaataaata tatgtgcaca cacaccatca gttcattggg ttgatgttta atcctagatt
     3841 caattgtgtt gagagctggg gactttaggt gatgtgtcca gacgctgaga tttgtgtctt
     3901 aaagtcacca caggcctgta tgctaaagat gagttgacag tcaatagcgt aataagcatt
     3961 tagaagcctc ttttcataaa aagcaccaac tcttcacttg gacccacaag gtaaatatgt
     4021 gaaggtagaa agtctgtcag gtcttccttc atttggtctc tgtcttcttg gctgagattc
     4081 catcttgagt ctgcctaata tcatctgttc tcaaggctaa agggacagag ataagtcatt
     4141 atccttagtc tttctccaca gtcccggggt aatagacgca tacaagcagc tccctttgga
     4201 atgaattcat gtctacctta ttgtgacagt gattcaacca cagctataga catgtagttt
     4261 cccttactca tgtgccacca atagactgtt atatttctgg tcacttttta tggtacatac
     4321 ttcttccaag cagcttctgg acataagcaa atctcccttc aatatgttgt gaaggttact
     4381 gtataagtag agcacttaaa aatagtcact aggggctaga gaagttactc tgtggttcag
     4441 agtacttgtt gcttgtgcag tggaccaggg ttcagttccc agcagccaca tgatggtttg
     4501 caactagcaa ttactccagt tccaggggtt ctgatgccgc ctttttacct ctacaggcat
     4561 cacacacaca cacaaacata catgcaggca aaacatgaat acacacagag aaaataaatc
     4621 tttataaaaa tgtaaaagac agccactagt atatttgttg taagccttaa atgtattttg
     4681 gttcaatatc ttttcataat ttcctcaaat tggataaaaa cctctctttt ctgtccgaag
     4741 tcttaagagc aggtacaggt caaaatgtac ccttattgct gttcagggca cagtgccctg
     4801 gatgcttgag agctctctcc cttagtcaga attaatctat cctcatattt tatatgtcct
     4861 aatttcatta aggacattaa atatggtgta atgttcaatt ttaaaggtaa attaatagga
     4921 cagaagcaca ccctgaaaat gatcaataga tgaaacctca aattttctgg gagtatgtga
     4981 ggaaaatatt tttaaaagca ctgacttagt taattaggac ccattgtatc agggaactac
     5041 tggcacgagg aaagagtgta gtccttcaag atcatcagct ttagtttctc cttttttccc
     5101 cttaacattg aacctgaatt tgaaaccaca tctttttgga cagtctgtgt ggggatgagg
     5161 ctgactgtgg atgtatttat tcggtgattt ttagaactga actaatgaac gtaaaggtat
     5221 attctctatt gtcccagaga ctgtgatccg tgtgaagtgc tagtcatttg tgtggtttct
     5281 atttgcataa ttcaagctca aattaattaa gaaactgaaa gtcactcaga aaaagactat
     5341 gtgttcatat ttgggccact gtttatctgt ctttggggtt tcttgacctg tggttgaaat
     5401 ggtatgaaat agtatgattg caaactatct gtgcatgtac ctgtttgcat gcctattact
     5461 tagaaaaaca cttagttttt atcacataag taataaatgt atctaagagg aatcctccta
     5521 aattgcaatg tatttcatta aagtgaaaaa aaaacacttc cttttgatag actagtaaca
     5581 acaaatatga aattataaat caaatactgc taatatttta ggaaatgttt tttatagttt
     5641 aaaatatctc aaatttacat tcttatgaga gattaaccaa tctttattct aaacttttga
     5701 gaatagaatt ttattaattc caatatttga aatatttcat accttgtgga attctgtaga
     5761 tactttccat cattatcaaa ggaaagacat agaacgtatt cttcctgtct tcacatattt
     5821 tgcagcatgt tgaacaatct ctgtctaata acactgggca acaatgacat attgttgcta
     5881 atagtgatat aatttatcat catcaccgtc atcacccacc agcatcatca gacatagaat
     5941 ctgacctatc ccttccaggc tgcccccagt gccctcccac ttctgattgt cctgcctcta
     6001 actgccaagc actgggatta cagggctgct gtaacaggcc tgtcttatgc agacacagag
     6061 atcagacctg gagcttgctg cgtgctagac aaacattctg ccaactgagt ggcatcctca
     6121 cacatgttat ctaaaggtgt ccactgccca taatagagct gtgttccagg agttctccag
     6181 tgcagagcta tagcaggtca cttttggaat tgtattctta aatgtgttta taaaaatgcc
     6241 aaaatctagc ccctgttctg gactgacttg ctctggtttc ttttgtgttg ttgatctaaa
     6301 acagtaagcc tcatagttac tttgttattt atttgaactc tcttaataaa attgtgttca
     6361 gacagttg
//
