LOCUS       NM_001417865            2754 bp    mRNA    linear   ROD 12-AUG-2024
DEFINITION  Mus musculus triokinase, FMN cyclase (Tkfc), transcript variant 2,
            mRNA.
ACCESSION   NM_001417865 XM_006526942
VERSION     NM_001417865.1
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2754)
  AUTHORS   Tsukamoto,R., Watanabe,K., Kodaka,M., Iwase,M., Sakiyama,H.,
            Inoue,Y., Suzuki,T., Yamamoto,Y., Shimizu,M., Sato,R. and Inoue,J.
  TITLE     HNF4alpha is required for Tkfc promoter activation by ChREBP
  JOURNAL   Biosci Biotechnol Biochem 88 (8), 941-947 (2024)
   PUBMED   38782732
  REMARK    GeneRIF: HNF4alpha is required for Tkfc promoter activation by
            ChREBP.
REFERENCE   2  (bases 1 to 2754)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   3  (bases 1 to 2754)
  AUTHORS   Ribeiro,J.M., Costas,M.J., Cabezas,A., Meunier,B., Onoufriadis,A.
            and Cameselle,J.C.
  TITLE     The TKFC Ala185Thr variant, reported as 'null' for fructose
            metabolism, is fully active as triokinase
  JOURNAL   FEBS Lett 596 (11), 1453-1457 (2022)
   PUBMED   35114011
  REMARK    GeneRIF: The TKFC Ala185Thr variant, reported as 'null' for
            fructose metabolism, is fully active as triokinase.
REFERENCE   4  (bases 1 to 2754)
  AUTHORS   Liu,L., Li,T., Liao,Y., Wang,Y., Gao,Y., Hu,H., Huang,H., Wu,F.,
            Chen,Y.G., Xu,S. and Fu,S.
  TITLE     Triose Kinase Controls the Lipogenic Potential of Fructose and
            Dietary Tolerance
  JOURNAL   Cell Metab 32 (4), 605-618 (2020)
   PUBMED   32818435
REFERENCE   5  (bases 1 to 2754)
  AUTHORS   Lo,J.C., Jamsai,D., O'Connor,A.E., Borg,C., Clark,B.J.,
            Whisstock,J.C., Field,M.C., Adams,V., Ishikawa,T., Aitken,R.J.,
            Whittle,B., Goodnow,C.C., Ormandy,C.J. and O'Bryan,M.K.
  TITLE     RAB-like 2 has an essential role in male fertility, sperm
            intra-flagellar transport, and tail assembly
  JOURNAL   PLoS Genet 8 (10), e1002969 (2012)
   PUBMED   23055941
REFERENCE   6  (bases 1 to 2754)
  AUTHORS   Hagopian,K., Ramsey,J.J. and Weindruch,R.
  TITLE     Enzymes of glycerol and glyceraldehyde metabolism in mouse liver:
            effects of caloric restriction and age on activities
  JOURNAL   Biosci Rep 28 (2), 107-115 (2008)
   PUBMED   18429748
REFERENCE   7  (bases 1 to 2754)
  AUTHORS   Hagopian,K., Ramsey,J.J. and Weindruch,R.
  TITLE     Fructose metabolizing enzymes from mouse liver: influence of age
            and caloric restriction
  JOURNAL   Biochim Biophys Acta 1721 (1-3), 37-43 (2005)
   PUBMED   15652177
REFERENCE   8  (bases 1 to 2754)
  AUTHORS   Massillon,D., Stalmans,W., van de Werve,G. and Bollen,M.
  TITLE     Identification of the glycogenic compound 5-iodotubercidin as a
            general protein kinase inhibitor
  JOURNAL   Biochem J 299 (Pt 1) (Pt 1), 123-128 (1994)
   PUBMED   8166629
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC125093.4.
            
            On Mar 10, 2023 this sequence version replaced XM_006526942.3.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR17253011.157653.1,
                                           SRR13422598.1225037.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849380 [ECO:0000348]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-485               AC125093.4         5927-6411
            486-591             AC125093.4         7547-7652
            592-781             AC125093.4         11060-11249
            782-892             AC125093.4         12402-12512
            893-1074            AC125093.4         12607-12788
            1075-1153           AC125093.4         14432-14510
            1154-1243           AC125093.4         14799-14888
            1244-1278           AC125093.4         15428-15462
            1279-1363           AC125093.4         15538-15622
            1364-1453           AC125093.4         15719-15808
            1454-1570           AC125093.4         16410-16526
            1571-1690           AC125093.4         16998-17117
            1691-1828           AC125093.4         17252-17389
            1829-1935           AC125093.4         18044-18150
            1936-2039           AC125093.4         18253-18356
            2040-2073           AC125093.4         18461-18494
            2074-2163           AC125093.4         18584-18673
            2164-2754           AC125093.4         19031-19621
FEATURES             Location/Qualifiers
     source          1..2754
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="19"
                     /map="19 6.6 cM"
     gene            1..2754
                     /gene="Tkfc"
                     /gene_synonym="Dak"
                     /note="triokinase, FMN cyclase"
                     /db_xref="GeneID:225913"
                     /db_xref="MGI:MGI:2385084"
     exon            1..485
                     /gene="Tkfc"
                     /gene_synonym="Dak"
                     /inference="alignment:Splign:2.1.0"
     exon            486..591
                     /gene="Tkfc"
                     /gene_synonym="Dak"
                     /inference="alignment:Splign:2.1.0"
     CDS             589..2325
                     /gene="Tkfc"
                     /gene_synonym="Dak"
                     /EC_number="2.7.1.29"
                     /EC_number="4.6.1.15"
                     /EC_number="2.7.1.28"
                     /note="isoform 1 is encoded by transcript variant 2;
                     bifunctional ATP-dependent dihydroxyacetone kinase/FAD-AMP
                     lyase (cyclizing); dihydroxyacetone kinase 2 homolog"
                     /codon_start=1
                     /product="triokinase/FMN cyclase isoform 1"
                     /protein_id="NP_001404794.1"
                     /db_xref="GeneID:225913"
                     /db_xref="MGI:MGI:2385084"
                     /translation="MSSKKMVNSVEGCADDALAGLVASNPDLQLLQGHRVALRSDLDT
                     LKGRVALLSGGGSGHEPAHAGFIGKGMLTGVIAGSVFASPPVGSILAAIRAVAQAGTV
                     GTLLIVKNYTGDRLNFGLAMEQAKAEGISVEMVIVEDDSAFTVLKKAGRRGLCGTVLI
                     HKVAGALAEEGMGLEEITKRVSVIAKTMGTLGVSLSSCSVPGATHTFELAADEIELGL
                     GIHGEAGVRRIKIAPVDQIVTLMLDHMTNTSNIFHVPVRSGSSVVLIVNNLGGLSFLE
                     LGIIADAAIRLLEGRGVKVARALVGTFMSALEMPGVSLTLMLVDEPVLKLIDAETTAK
                     AWPHMAKVSVTGRKRIRAAPTEPPEAPEATAAGGVTSKQMALVLDRICTTLIGLEEHL
                     NALDRAAGDGDCGSTHSRAAKAIQGWLKEGPSLTSPAQVLSRLSVLLLERMGGSSGAL
                     YGLFLTAAAQPLKAKTDLPTWSAAMDAGLESMQKYGKAAPGDRTMLDSLWAAAQEFQA
                     WKSPGASLLPVLTKAVKSAEAAAEATKNMEAGAGRASYISSAQLDQPDPGAVAAAAIF
                     RAILEVLQTQGA"
     exon            592..781
                     /gene="Tkfc"
                     /gene_synonym="Dak"
                     /inference="alignment:Splign:2.1.0"
     exon            782..892
                     /gene="Tkfc"
                     /gene_synonym="Dak"
                     /inference="alignment:Splign:2.1.0"
     exon            893..1074
                     /gene="Tkfc"
                     /gene_synonym="Dak"
                     /inference="alignment:Splign:2.1.0"
     exon            1075..1153
                     /gene="Tkfc"
                     /gene_synonym="Dak"
                     /inference="alignment:Splign:2.1.0"
     exon            1154..1243
                     /gene="Tkfc"
                     /gene_synonym="Dak"
                     /inference="alignment:Splign:2.1.0"
     exon            1244..1278
                     /gene="Tkfc"
                     /gene_synonym="Dak"
                     /inference="alignment:Splign:2.1.0"
     exon            1279..1363
                     /gene="Tkfc"
                     /gene_synonym="Dak"
                     /inference="alignment:Splign:2.1.0"
     exon            1364..1453
                     /gene="Tkfc"
                     /gene_synonym="Dak"
                     /inference="alignment:Splign:2.1.0"
     exon            1454..1570
                     /gene="Tkfc"
                     /gene_synonym="Dak"
                     /inference="alignment:Splign:2.1.0"
     exon            1571..1690
                     /gene="Tkfc"
                     /gene_synonym="Dak"
                     /inference="alignment:Splign:2.1.0"
     exon            1691..1828
                     /gene="Tkfc"
                     /gene_synonym="Dak"
                     /inference="alignment:Splign:2.1.0"
     exon            1829..1935
                     /gene="Tkfc"
                     /gene_synonym="Dak"
                     /inference="alignment:Splign:2.1.0"
     exon            1936..2039
                     /gene="Tkfc"
                     /gene_synonym="Dak"
                     /inference="alignment:Splign:2.1.0"
     exon            2040..2073
                     /gene="Tkfc"
                     /gene_synonym="Dak"
                     /inference="alignment:Splign:2.1.0"
     exon            2074..2163
                     /gene="Tkfc"
                     /gene_synonym="Dak"
                     /inference="alignment:Splign:2.1.0"
     exon            2164..2754
                     /gene="Tkfc"
                     /gene_synonym="Dak"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2735..2740
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Tkfc"
                     /gene_synonym="Dak"
                     /note="hexamer: AATAAA"
     polyA_site      2754
                     /gene="Tkfc"
                     /gene_synonym="Dak"
                     /note="major polyA site"
ORIGIN      
        1 gttccgcgac tgccagcgcc ttccattccc ggagcccaag tttttgctcc cgccgcccct
       61 ggggccgctg gcctccctag gcctgggatc cctcccttgc ccgggtctcc ggccggggca
      121 atctcaccgg tcacacagcc gttcactgca gtaggcttct gcgccgttac gacgtagttg
      181 tacgacatat tgaggccttg ggttggcccg tggggcgcct agagcgacga tagagagacg
      241 ggcgcgagcg aagagactga gaacccccac cctcacgcag ccaactccgt ggctgctgcc
      301 tccgcccctg agacacgttg caggccagag aggccaggcg cagaggatca cgggacggcc
      361 tcacctggcc tcttggagga cttacggagc ccgaggcggc caaccaagcg aggccccgcc
      421 ccctgaggtc ccgccttctc ctaaccgcca gaggtcgcag tacccggatg agagcgagag
      481 taccggtgct gctgtctcta cttcagacct ggtagcagtc aggaccgtcc acccggcggc
      541 atctcagagc agaagtgtga gctcagcttg tttcagaact ttcccaccat gagttccaag
      601 aagatggtga attctgtgga agggtgtgct gatgatgccc ttgctgggtt agtagcctct
      661 aaccctgact tgcagctcct gcaagggcac cgtgtggccc tacgttctga cctggacacc
      721 ctcaagggcc gggtagcact tctgtcaggt ggaggctcag gccatgagcc tgcccatgcc
      781 ggttttatcg ggaaagggat gctaacaggt gtcatcgcag gatctgtgtt tgcctctcct
      841 cctgtgggca gcatcctggc agccattaga gctgtggccc aggcaggcac agtgggcacc
      901 ctcctcattg tgaagaacta cactggggat cggctcaact ttggacttgc catggagcag
      961 gccaaggccg agggcatctc tgtggagatg gtgatagttg aggatgacag tgccttcacc
     1021 gtcctcaaga aggcaggccg gcgtggcctg tgtggcacag tacttatcca caaggtggca
     1081 ggtgctctgg ctgaggaagg aatggggctg gaggagatca caaagagggt gagcgtgatc
     1141 gccaagacca tgggtaccct aggggtgagc ttgtcatcct gcagtgtccc tggcgccaca
     1201 cacacctttg agcttgcagc tgatgaaata gagctaggcc tggggatcca tggggaagct
     1261 ggtgttcgtc ggataaagat agcacctgtt gatcagattg tcactcttat gcttgaccac
     1321 atgacaaaca cctccaatat atttcatgtg cctgtacggt caggctcttc agtggtgctg
     1381 atagtcaaca atctgggtgg cctgtctttc ctggaactgg gcatcatagc tgatgctgcc
     1441 atacgcttgc tggagggccg tggagtgaag gtcgcccgtg ccctggtggg caccttcatg
     1501 tcagcgctgg agatgcctgg tgtgtctctt actttgatgc ttgtggatga acccgtgctg
     1561 aagctaatag atgctgaaac tactgcaaaa gcctggcctc acatggccaa ggtctctgtg
     1621 actgggagga agcggattcg ggcagctccc acagagcctc cagaagcccc tgaagccact
     1681 gcagcaggag gtgtaacatc aaagcagatg gcacttgtgc tggatcggat atgcaccacc
     1741 ctcatcggac tggaggagca cctgaatgcc ttggacaggg ctgctggtga tggggattgt
     1801 ggttctaccc atagccgtgc tgccaaagcc attcagggct ggctaaagga aggcccatct
     1861 ctaaccagcc ctgcccaggt actctccaga ttgtctgtcc tgctgctgga gaggatggga
     1921 ggctcatctg gggcgctcta tggcctgttc ctgactgcag ctgcccagcc tctcaaggcc
     1981 aagactgacc tcccaacctg gtctgctgcc atggatgccg gcttagagtc catgcagaag
     2041 tatggaaagg ctgcaccagg agacaggaca atgctggatt ctctgtgggc agcagcacag
     2101 gagttccaag cctggaagag cccaggggcc agtctcctcc cagtcctgac taaagcagtc
     2161 aagagtgctg aagctgcagc cgaggccacc aagaatatgg aagctggtgc tgggagagct
     2221 agttatatca gctctgcaca actagatcag ccagaccctg gagcagtcgc agctgctgcc
     2281 atcttccgtg ccatcctgga ggtcttgcag acgcagggag catgactgct tttctcctcc
     2341 tcagctcatt tgttactttg tcttgccttc agatggtcac ctccctccct ccctccttcc
     2401 ctcccccttc ccccaccctc cccctaacac ccaacctctg catggaacag gacatccttc
     2461 accacacctt cagaactgtt ccatgaagat cgtatctggc taggtgggtc acatgtccgg
     2521 gcagcactgt tcttcccctc ctcaggatga ggagtccctc agatcttatc caccccagtc
     2581 tctaactggg tatacccatt tcgtttcaag atttcaggcc tcagcctggc agtggtggcg
     2641 cacgccttta atcccagcac ttgggaggca aaaagattct aggcctcttc ctctggaagg
     2701 cctcctatac ccagtcagta tacgaaagaa agttaataaa atccattgaa gcca
//
