LOCUS       NM_021028               2400 bp    mRNA    linear   ROD 09-FEB-2025
DEFINITION  Mus musculus thymidine kinase 2, mitochondrial (Tk2), transcript
            variant 1, mRNA; nuclear gene for mitochondrial product.
ACCESSION   NM_021028
VERSION     NM_021028.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2400)
  AUTHORS   Zhao,Q., Zhou,X., Yan,J., Kuiper,R., Curbo,S. and Karlsson,A.
  TITLE     Long term survival and abnormal liver fat accumulation in mice with
            specific thymidine kinase 2 deficiency in liver tissue
  JOURNAL   PLoS One 18 (10), e0285242 (2023)
   PUBMED   37796969
  REMARK    GeneRIF: Long term survival and abnormal liver fat accumulation in
            mice with specific thymidine kinase 2 deficiency in liver tissue.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2400)
  AUTHORS   Siegmund,S.E., Yang,H., Sharma,R., Javors,M., Skinner,O.,
            Mootha,V., Hirano,M. and Schon,E.A.
  TITLE     Low-dose rapamycin extends lifespan in a mouse model of mtDNA
            depletion syndrome
  JOURNAL   Hum Mol Genet 26 (23), 4588-4605 (2017)
   PUBMED   28973153
REFERENCE   3  (bases 1 to 2400)
  AUTHORS   Bartesaghi,S., Graziano,V., Galavotti,S., Henriquez,N.V., Betts,J.,
            Saxena,J., Minieri,V., A,D., Karlsson,A., Martins,L.M., Capasso,M.,
            Nicotera,P., Brandner,S., De Laurenzi,V. and Salomoni,P.
  TITLE     Inhibition of oxidative metabolism leads to p53 genetic
            inactivation and transformation in neural stem cells
  JOURNAL   Proc Natl Acad Sci U S A 112 (4), 1059-1064 (2015)
   PUBMED   25583481
  REMARK    Erratum:[Proc Natl Acad Sci U S A. 2015 Mar 17;112(11):E1401. doi:
            10.1073/pnas.1503132112.. Minieri, Valentina [added]. PMID:
            25713129]
REFERENCE   4  (bases 1 to 2400)
  AUTHORS   Krishnan,S., Paredes,J.A., Zhou,X., Kuiper,R.V., Hultenby,K.,
            Curbo,S. and Karlsson,A.
  TITLE     Long term expression of Drosophila melanogaster nucleoside kinase
            in thymidine kinase 2-deficient mice with no lethal effects caused
            by nucleotide pool imbalances
  JOURNAL   J Biol Chem 289 (47), 32835-32844 (2014)
   PUBMED   25296759
  REMARK    GeneRIF: Data indicate that long term Dm-dNK ( nucleoside kinase
            from Drosophila melanogaster) expression rescued thymidine kinase 2
            (Tk2)-deficient mice without lethal side effects.
REFERENCE   5  (bases 1 to 2400)
  AUTHORS   Garone,C., Garcia-Diaz,B., Emmanuele,V., Lopez,L.C., Tadesse,S.,
            Akman,H.O., Tanji,K., Quinzii,C.M. and Hirano,M.
  TITLE     Deoxypyrimidine monophosphate bypass therapy for thymidine kinase 2
            deficiency
  JOURNAL   EMBO Mol Med 6 (8), 1016-1027 (2014)
   PUBMED   24968719
REFERENCE   6  (bases 1 to 2400)
  AUTHORS   Mootha,V.K., Bunkenborg,J., Olsen,J.V., Hjerrild,M.,
            Wisniewski,J.R., Stahl,E., Bolouri,M.S., Ray,H.N., Sihag,S.,
            Kamal,M., Patterson,N., Lander,E.S. and Mann,M.
  TITLE     Integrated analysis of protein composition, tissue diversity, and
            gene regulation in mouse mitochondria
  JOURNAL   Cell 115 (5), 629-640 (2003)
   PUBMED   14651853
REFERENCE   7  (bases 1 to 2400)
  AUTHORS   Da Cruz,S., Xenarios,I., Langridge,J., Vilbois,F., Parone,P.A. and
            Martinou,J.C.
  TITLE     Proteomic analysis of the mouse liver mitochondrial inner membrane
  JOURNAL   J Biol Chem 278 (42), 41566-41571 (2003)
   PUBMED   12865426
REFERENCE   8  (bases 1 to 2400)
  AUTHORS   Saada,A., Shaag,A., Mandel,H., Nevo,Y., Eriksson,S. and Elpeleg,O.
  TITLE     Mutant mitochondrial thymidine kinase in mitochondrial DNA
            depletion myopathy
  JOURNAL   Nat Genet 29 (3), 342-344 (2001)
   PUBMED   11687801
REFERENCE   9  (bases 1 to 2400)
  AUTHORS   Wang,L. and Eriksson,S.
  TITLE     Cloning and characterization of full-length mouse thymidine kinase
            2: the N-terminal sequence directs import of the precursor protein
            into mitochondria
  JOURNAL   Biochem J 351 Pt 2 (Pt 2), 469-476 (2000)
   PUBMED   11023833
REFERENCE   10 (bases 1 to 2400)
  AUTHORS   Wettin,K., Johansson,M., Zheng,X., Zhu,C. and Karlsson,A.
  TITLE     Cloning of mouse mitochondrial thymidine kinase 2 cDNA
  JOURNAL   FEBS Lett 460 (1), 103-106 (1999)
   PUBMED   10571069
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK087557.1 and AV240875.1.
            
            On Mar 21, 2009 this sequence version replaced NM_021028.2.
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes a protein.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK087557.1, AK154160.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164139 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: reported by MitoCarta
            RefSeq Select criteria             :: based on conservation,
                                                  expression, longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-979               AK087557.1         2-980
            980-2387            AK087557.1         982-2389
            2388-2400           AV240875.1         251-263
FEATURES             Location/Qualifiers
     source          1..2400
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="8"
                     /map="8 53.04 cM"
     gene            1..2400
                     /gene="Tk2"
                     /gene_synonym="mt-TK"
                     /note="thymidine kinase 2, mitochondrial"
                     /db_xref="GeneID:57813"
                     /db_xref="MGI:MGI:1913266"
     exon            1..158
                     /gene="Tk2"
                     /gene_synonym="mt-TK"
                     /inference="alignment:Splign:2.1.0"
     CDS             26..838
                     /gene="Tk2"
                     /gene_synonym="mt-TK"
                     /EC_number="2.7.1.74"
                     /EC_number="2.7.1.21"
                     /note="deoxycytidine kinase TK2; 2'-deoxyuridine kinase
                     TK2"
                     /codon_start=1
                     /product="thymidine kinase 2, mitochondrial precursor"
                     /protein_id="NP_066356.3"
                     /db_xref="CCDS:CCDS22573.1"
                     /db_xref="GeneID:57813"
                     /db_xref="MGI:MGI:1913266"
                     /translation="MLLRSLRSWAARSLRSVGPGSSGSPGSLDSGAGPLWAPRRAWPP
                     DKDRENDKEKKAVVCIEGNIASGKTTCLEFFSNTTDVEVLMEPVLKWRNVHGHNPLSL
                     MYHDASRWGLTLQTYVQLTMLDQHTRPQMSPVRLMERSIYSARYIFVENLYRSGKMPE
                     VDYAILSEWFDWIVRNIDVSVDLIVYLRTTPEICYQRLKMRCREEEKVIPMEYLHAIH
                     RLYEEWLVNGSLFPAAAPVLVIEADHNLEKMLELFEQNRARILTPENWKHGP"
     exon            159..196
                     /gene="Tk2"
                     /gene_synonym="mt-TK"
                     /inference="alignment:Splign:2.1.0"
     exon            197..271
                     /gene="Tk2"
                     /gene_synonym="mt-TK"
                     /inference="alignment:Splign:2.1.0"
     exon            272..325
                     /gene="Tk2"
                     /gene_synonym="mt-TK"
                     /inference="alignment:Splign:2.1.0"
     exon            326..415
                     /gene="Tk2"
                     /gene_synonym="mt-TK"
                     /inference="alignment:Splign:2.1.0"
     exon            416..489
                     /gene="Tk2"
                     /gene_synonym="mt-TK"
                     /inference="alignment:Splign:2.1.0"
     exon            490..578
                     /gene="Tk2"
                     /gene_synonym="mt-TK"
                     /inference="alignment:Splign:2.1.0"
     exon            579..658
                     /gene="Tk2"
                     /gene_synonym="mt-TK"
                     /inference="alignment:Splign:2.1.0"
     exon            659..739
                     /gene="Tk2"
                     /gene_synonym="mt-TK"
                     /inference="alignment:Splign:2.1.0"
     exon            740..2400
                     /gene="Tk2"
                     /gene_synonym="mt-TK"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2372..2377
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Tk2"
                     /gene_synonym="mt-TK"
                     /note="hexamer: AATAAA"
     polyA_site      2391
                     /gene="Tk2"
                     /gene_synonym="mt-TK"
                     /note="major polyA site"
ORIGIN      
        1 aggacttgca catcagccag ggactatgct gctgcggtcg ctgcgtagtt gggctgcccg
       61 gtccctgcgt agcgtgggcc cggggagctc cgggagcccc gggagcctcg attcgggcgc
      121 cgggccgctg tgggcacctc gccgcgcctg gcctccggat aaagatagag aaaatgataa
      181 ggagaaaaaa gcagtggttt gtattgaggg caatattgca agtgggaaga cgacatgcct
      241 ggagttcttc tccaatacaa cagacgtcga ggtgttaatg gagcctgtgc tcaagtggag
      301 aaatgtccat ggccataacc ctctgagcct catgtaccat gatgccagcc gatggggcct
      361 cacactgcag acgtacgtgc agctcaccat gctggaccag cacacgcgcc ctcagatgtc
      421 acctgtacgg ttgatggaaa ggtcaattta cagcgcaaga tacatttttg tagaaaacct
      481 gtatagaagt gggaagatgc ccgaggttga ctacgcgatt ctgtctgagt ggtttgactg
      541 gatcgtcagg aacattgatg tctctgttga tctgatagtt tatctgcgaa ccactcccga
      601 aatctgctac cagagattaa agatgaggtg ccgggaagag gagaaagtca ttccgatgga
      661 atacctccat gctattcacc gcctctacga ggagtggctg gtcaacggga gcctcttccc
      721 agctgcagcc cctgttctgg tgattgaggc tgaccacaac ttggagaaaa tgttagaact
      781 ctttgaacaa aaccgggccc ggatattaac tccagagaac tggaagcatg gaccctagga
      841 ctgagtgatc tacaagacag cgccaggaac agcccagcca gggccaggtt agctgctagg
      901 agcatttgga agaatctact cttaggaggg tctgttatct aatcagatct attttctcaa
      961 tggtcttttc actgcagtac aaatggcttt tgtctgtcag atgcttgcag catacatccc
     1021 tcctggtgcc cctcctggtg gcttcctaac gatgaagccc tgtgttaaaa tcactgactg
     1081 tatccctggg gttgttctgg atcctctgca tcaggtcacc tcatgggtga gggagcagtc
     1141 ttccacccca ggcaaggatt tgaagcccag agaggtgtgg ggactcgtca agccagagca
     1201 ttgtggaggg agctgggacc attcagatac ctggtttggg ctgcctcctc ctaagctgtt
     1261 ccccaccgcc ctgctgctct gagcaccagc ccccgcctct gtgagaagag tggacagttt
     1321 gtgaattcca tcatctttca gtaccgggcc agttcaagga gaccagacat cactaggtgt
     1381 tggcacacct ctcttatttg gcatgagccc agggtgtcct ggtatgaggg tactgtgacc
     1441 tctatccatt cctttgtcac ctggacctcg gtgtccactg gggactcctg gatcggcctc
     1501 tgcttggtgc cagagtcctt gaggatgagt cctccccaga ctctaccagt tccctgtcgt
     1561 tttgtgtcag aaaaaagggg gttatgggac ctggacactt taccctttgc tgagcgaggc
     1621 caagggcagc cagctgagct tccaagaccc catcactctc tcctgtcctt gtgtgaggag
     1681 cagtgtcccc ttgctccagg gagagtgaca gtggccttaa ctcctttgaa gtgtttttcc
     1741 atctgagcag atgtcatgga ccacgagcat gaagaagtca tctctattgg atcagaaaat
     1801 accttagctt tgctgagccg taactgtgca ctgaaggtgc agctgttact tgaatcagtc
     1861 atgaccaaga gaggccggcc attcctcagg accgtgagac tggaaagtca tggtttgcca
     1921 acaaagacaa tttaagcctt gctgctcttg acattctaga atggatggag tgcatctgga
     1981 aaagactgga cacagatgat acaggggaat gatgaccgta taccctggag aagcacagca
     2041 caggctgggg agggggctgc aggggagccc tgctcccctg tttagctgcc tgggagctag
     2101 aaagagggcc aagagtccag ctcttggtcc tttctgtttg gaccaattct tggccctttt
     2161 ggtagtatga agagtagcat ccagggagat tgtccatcag cacctgcagt ccagcaatgg
     2221 ataaacctga gaaaggcagg aactttgaac cagcagtact catgggggca tctttagaat
     2281 ttgcaaacga acagtctagt acttgccctt tgctctacca gaacacttgt tttggggaga
     2341 aatgtgctgt ttctctttct ctctagtagc caataaaatt ctacagctaa accatgatgt
//
