LOCUS       NM_009387               1389 bp    mRNA    linear   ROD 24-DEC-2024
DEFINITION  Mus musculus thymidine kinase 1 (Tk1), transcript variant 1, mRNA.
ACCESSION   NM_009387
VERSION     NM_009387.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1389)
  AUTHORS   Wu,M. and Ye,M.
  TITLE     Transcription factor Dp-1 knockdown downregulates thymidine kinase
            1 expression to protect against proliferation and
            epithelial-mesenchymal transition in cervical cancer
  JOURNAL   Funct Integr Genomics 23 (4), 301 (2023)
   PUBMED   37715794
  REMARK    GeneRIF: Transcription factor Dp-1 knockdown downregulates
            thymidine kinase 1 expression to protect against proliferation and
            epithelial-mesenchymal transition in cervical cancer.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1389)
  AUTHORS   Bedogni,F. and Hevner,R.F.
  TITLE     Cell-Type-Specific Gene Expression in Developing Mouse Neocortex:
            Intermediate Progenitors Implicated in Axon Development
  JOURNAL   Front Mol Neurosci 14, 686034 (2021)
   PUBMED   34321999
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 1389)
  AUTHORS   Ingham,N.J., Pearson,S.A., Vancollie,V.E., Rook,V., Lewis,M.A.,
            Chen,J., Buniello,A., Martelletti,E., Preite,L., Lam,C.C.,
            Weiss,F.D., Powis,Z., Suwannarat,P., Lelliott,C.J., Dawson,S.J.,
            White,J.K. and Steel,K.P.
  TITLE     Mouse screen reveals multiple new genes underlying mouse and human
            hearing loss
  JOURNAL   PLoS Biol 17 (4), e3000194 (2019)
   PUBMED   30973865
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 1389)
  AUTHORS   Loo,L., Simon,J.M., Xing,L., McCoy,E.S., Niehaus,J.K., Guo,J.,
            Anton,E.S. and Zylka,M.J.
  TITLE     Single-cell transcriptomic analysis of mouse neocortical
            development
  JOURNAL   Nat Commun 10 (1), 134 (2019)
   PUBMED   30635555
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 1389)
  AUTHORS   Mathur,V., Burai,R., Vest,R.T., Bonanno,L.N., Lehallier,B.,
            Zardeneta,M.E., Mistry,K.N., Do,D., Marsh,S.E., Abud,E.M.,
            Blurton-Jones,M., Li,L., Lashuel,H.A. and Wyss-Coray,T.
  TITLE     Activation of the STING-Dependent Type I Interferon Response
            Reduces Microglial Reactivity and Neuroinflammation
  JOURNAL   Neuron 96 (6), 1290-1302 (2017)
   PUBMED   29268096
REFERENCE   6  (bases 1 to 1389)
  AUTHORS   Wintersberger,E., Rotheneder,H., Grabner,M., Beck,G. and Seiser,C.
  TITLE     Regulation of thymidine kinase during growth, cell cycle and
            differentiation
  JOURNAL   Adv Enzyme Regul 32, 241-254 (1992)
   PUBMED   1496920
REFERENCE   7  (bases 1 to 1389)
  AUTHORS   Ambrose,C., Cheng,S., Fontaine,B., Nadeau,J.H., MacDonald,M. and
            Gusella,J.F.
  TITLE     The alpha-subunit of the skeletal muscle sodium channel is encoded
            proximal to Tk-1 on mouse chromosome 11
  JOURNAL   Mamm Genome 3 (3), 151-155 (1992)
   PUBMED   1352160
REFERENCE   8  (bases 1 to 1389)
  AUTHORS   Lalley,P.A., Minna,J.D. and Francke,U.
  TITLE     Conservation of autosomal gene synteny groups in mouse and man
  JOURNAL   Nature 274 (5667), 160-163 (1978)
   PUBMED   662012
REFERENCE   9  (bases 1 to 1389)
  AUTHORS   Kozak,C.A. and Ruddle,F.H.
  TITLE     Assignment of the genes for thymidine kinase and galactokinase to
            Mus musculus chromosome 11 and the preferential segregation of this
            chromosome in Chinese hamster/mouse somatic cell hybrids
  JOURNAL   Somatic Cell Genet 3 (2), 121-133 (1977)
   PUBMED   204065
REFERENCE   10 (bases 1 to 1389)
  AUTHORS   McBreen,P., Orkwiszewski,K.G., Chern,C.J., Mellman,W.J. and
            Croce,C.M.
  TITLE     Synteny of the genes for thymidine kinase and galactokinase in the
            mouse and their assignment to mouse chromosome 11
  JOURNAL   Cytogenet Cell Genet 19 (1), 7-13 (1977)
   PUBMED   196814
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BY773001.1, AK139244.1 and AA047942.1.
            
            On Apr 18, 2009 this sequence version replaced NM_009387.1.
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Sequence Note: An in-frame AUG is located 23 codons upstream of the
            annotated translation start site but is not being annotated as a
            start site since it is not conserved and is in a weak Kozak
            sequence context.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC091758.1, AK085188.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164138, SAMN01164140
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            CDS uses downstream in-frame AUG :: upstream AUG and CDS extension
                                                is not conserved
            RefSeq Select criteria           :: based on conservation,
                                                expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-183               BY773001.1         1-183
            184-1361            AK139244.1         4-1181
            1362-1389           AA047942.1         429-456
FEATURES             Location/Qualifiers
     source          1..1389
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="11"
                     /map="11 82.96 cM"
     gene            1..1389
                     /gene="Tk1"
                     /gene_synonym="D530002A18Rik; Tk-1; Tk1a; Tk1b"
                     /note="thymidine kinase 1"
                     /db_xref="GeneID:21877"
                     /db_xref="MGI:MGI:98763"
     exon            1..301
                     /gene="Tk1"
                     /gene_synonym="D530002A18Rik; Tk-1; Tk1a; Tk1b"
                     /inference="alignment:Splign:2.1.0"
     CDS             236..937
                     /gene="Tk1"
                     /gene_synonym="D530002A18Rik; Tk-1; Tk1a; Tk1b"
                     /EC_number="2.7.1.21"
                     /note="isoform 1 is encoded by transcript variant 1;
                     thymidine kinase, cytosolic"
                     /codon_start=1
                     /product="thymidine kinase, cytosolic isoform 1"
                     /protein_id="NP_033413.2"
                     /db_xref="CCDS:CCDS25692.1"
                     /db_xref="GeneID:21877"
                     /db_xref="MGI:MGI:98763"
                     /translation="MSYINLPTVLPSSPSKTRGQIQVILGPMFSGKSTELMRRVRRFQ
                     IAQYKCLVIKYAKDTRYSNSFSTHDRNTMDALPACMLRDVTQESLGVAVIGIDEGQFF
                     PDIVDFCEMMANEGKTVIVAALDGTFQRKAFGSILNLVPLAESVVKLTAVCMECFREA
                     AYTKRLGLEKEVEVIGGADKYHSVCRLCYFKKSSAQTAGSDNKNCLVLGQPGEALVVR
                     KLFASQQVLQYNSAN"
     exon            302..333
                     /gene="Tk1"
                     /gene_synonym="D530002A18Rik; Tk-1; Tk1a; Tk1b"
                     /inference="alignment:Splign:2.1.0"
     exon            334..444
                     /gene="Tk1"
                     /gene_synonym="D530002A18Rik; Tk-1; Tk1a; Tk1b"
                     /inference="alignment:Splign:2.1.0"
     exon            445..538
                     /gene="Tk1"
                     /gene_synonym="D530002A18Rik; Tk-1; Tk1a; Tk1b"
                     /inference="alignment:Splign:2.1.0"
     exon            539..628
                     /gene="Tk1"
                     /gene_synonym="D530002A18Rik; Tk-1; Tk1a; Tk1b"
                     /inference="alignment:Splign:2.1.0"
     exon            629..748
                     /gene="Tk1"
                     /gene_synonym="D530002A18Rik; Tk-1; Tk1a; Tk1b"
                     /inference="alignment:Splign:2.1.0"
     exon            749..1369
                     /gene="Tk1"
                     /gene_synonym="D530002A18Rik; Tk-1; Tk1a; Tk1b"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1342..1347
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Tk1"
                     /gene_synonym="D530002A18Rik; Tk-1; Tk1a; Tk1b"
                     /note="hexamer: ATTAAA"
     polyA_site      1365
                     /gene="Tk1"
                     /gene_synonym="D530002A18Rik; Tk-1; Tk1a; Tk1b"
                     /note="major polyA site"
ORIGIN      
        1 acacctacct ccgaagacaa gttcctccag ggattctggc ccgcagaaag ggaaggaaac
       61 gccatggcca gatccggagg ggatggtcga gctccaggct tttcacgtag ctgagaggtg
      121 ggacgagtct tgtcttcgtc ccgccccctt ttgagttcgc gggcaaatgc gagcagtaag
      181 tcgaaatttt tccacccacg gactctcggt gctaactaag gtttgcacag cagccatgag
      241 ctacatcaat ctgcccaccg tgctgcccag ctcccccagc aagactcggg ggcagattca
      301 ggtgattctc gggcccatgt tctcagggaa aagcacagag ctgatgagaa gagtccggcg
      361 cttccagatc gcccagtaca agtgcctggt catcaagtat gccaaagaca cgcgctatag
      421 caacagcttc tccacacatg atcggaacac catggacgca ttgccagcct gcatgctccg
      481 cgatgtgacc caggagtcct tgggtgtggc cgtcattggc atcgatgagg ggcagttttt
      541 tcctgacatt gtggatttct gtgaaatgat ggccaacgag ggcaagacag taattgtggc
      601 agcgctggat gggaccttcc agaggaaggc tttcggcagc atcttgaacc tggtgcccct
      661 ggcggagagt gtggtgaagc tcaccgctgt gtgcatggag tgcttccgag aagctgccta
      721 cacgaagagg ctgggcctgg agaaagaggt ggaggtgatt ggcggagccg acaagtatca
      781 ctccgtgtgc cgcctgtgct actttaagaa gtcttcagcc cagactgctg gctcagacaa
      841 caagaactgt ctggtgctgg ggcagccggg agaggccttg gttgtcagga agctctttgc
      901 ctctcagcaa gtcctacaat acaactctgc caactgaggg gacctgaggc ctgccagctc
      961 ctacccaggt tggactctca gagagcaggg ggagcgcggg cctgccattc ctaatggaca
     1021 atgtaccttg aacaggctgc cactcgctga agccgttttc agttcccttc ttgattgcca
     1081 agatgcctca atgcagactg gagcccagac cctgcctggt ggctagcggt cccgtgttca
     1141 gccaaaggtg aggacagagc tgtccagcat tgtgacactg gtggggctag tttcttcctt
     1201 gttggtggct gggtttcagt ctcagagccc caccctcacc aaggctccag gcctctcaca
     1261 gctcccccat ttatgcctaa acattctctc ctcagaacct cagctcttag tgagccactt
     1321 ttcttgtgca aaatgaacaa tattaaagtt tactactaat gagaacgtga aaaaaaaaaa
     1381 aaaaaaaaa
//
