LOCUS       NM_001252474            2462 bp    mRNA    linear   ROD 04-JUN-2024
DEFINITION  Mus musculus tight junction associated protein 1 (Tjap1),
            transcript variant 3, mRNA.
ACCESSION   NM_001252474
VERSION     NM_001252474.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2462)
  AUTHORS   Tamaki,H., Sanda,M., Katsumata,O., Hara,Y., Fukaya,M. and
            Sakagami,H.
  TITLE     Pilt is a coiled-coil domain-containing protein that localizes at
            the trans-Golgi complex and regulates its structure
  JOURNAL   FEBS Lett 586 (19), 3064-3070 (2012)
   PUBMED   22841714
REFERENCE   2  (bases 1 to 2462)
  AUTHORS   Kawabe,H., Nakanishi,H., Asada,M., Fukuhara,A., Morimoto,K.,
            Takeuchi,M. and Takai,Y.
  TITLE     Pilt, a novel peripheral membrane protein at tight junctions in
            epithelial cells
  JOURNAL   J Biol Chem 276 (51), 48350-48355 (2001)
   PUBMED   11602598
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC116739.13 and AK164925.1.
            
            Transcript Variant: This variant (3) differs in the 5' UTR compared
            to variant 1. Variants 1, 2, 3 and 4 encode the same protein.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK164925.1, SRR13948564.2787467.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-16                AC116739.13        10675-10690
            17-672              AK164925.1         2-657
            673-2442            AK164925.1         659-2428
            2443-2462           AC116739.13        35831-35850
FEATURES             Location/Qualifiers
     source          1..2462
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="17"
                     /map="17 22.9 cM"
     gene            1..2462
                     /gene="Tjap1"
                     /gene_synonym="0610041D19Rik; Pilt; Tjp4"
                     /note="tight junction associated protein 1"
                     /db_xref="GeneID:74094"
                     /db_xref="MGI:MGI:1921344"
     exon            1..67
                     /gene="Tjap1"
                     /gene_synonym="0610041D19Rik; Pilt; Tjp4"
                     /inference="alignment:Splign:2.1.0"
     exon            68..161
                     /gene="Tjap1"
                     /gene_synonym="0610041D19Rik; Pilt; Tjp4"
                     /inference="alignment:Splign:2.1.0"
     exon            162..257
                     /gene="Tjap1"
                     /gene_synonym="0610041D19Rik; Pilt; Tjp4"
                     /inference="alignment:Splign:2.1.0"
     exon            258..379
                     /gene="Tjap1"
                     /gene_synonym="0610041D19Rik; Pilt; Tjp4"
                     /inference="alignment:Splign:2.1.0"
     CDS             281..1900
                     /gene="Tjap1"
                     /gene_synonym="0610041D19Rik; Pilt; Tjp4"
                     /note="tight junction protein 4 (peripheral); hdlg-binding
                     protein Pilt; protein incorporated later into tight
                     junctions"
                     /codon_start=1
                     /product="tight junction-associated protein 1"
                     /protein_id="NP_001239403.1"
                     /db_xref="CCDS:CCDS28824.1"
                     /db_xref="GeneID:74094"
                     /db_xref="MGI:MGI:1921344"
                     /translation="MSSAAPAKKPYRKAPPEHRELRLEIPVSRLEQEESLTDAERMKL
                     LQQENEELRKRLASATRRTEALERELEIGQDCLELELGQSREELDKFKDKFRRLQNSY
                     TASQRTNQELEDKLHALIKKAEMDRKTLDWEIVELTNKLLDARNTINKLEELNERYRL
                     DCNLAVQLLKCNKSHFRNHKLADLPCELQDMVRKHLRSGQEVASPSPSPSSSLSPGAV
                     VPTSVIARVLEKPESLLLNSAQSGSAGRPLAEDVFVHVDMSGGDPASPPAPGSPNGEC
                     CSVSTAGGSPEEELPLPAFDKLSPYPTPSPPHPLYPGRKVIEFSEDKIRIPRNSPLPN
                     CTYATRQAISLSLVEDGSERAHRSSVPSSPASAQGSPHHQPSPAPSALSAPASSASSE
                     EDLLASWQRAFVDRTPPPAAVVQRTAFGRDSLPELQLHFSPGHSTAPPPSPHRERGLV
                     LPAEPDSGFPQDEEEEMLNLPVSPEEERQSLLPDKEGTEEASGPSHVDGRAWPLPSPS
                     RPQRSPKRMGVHHLHRKDSLTQAQEQGTVLS"
     exon            380..408
                     /gene="Tjap1"
                     /gene_synonym="0610041D19Rik; Pilt; Tjp4"
                     /inference="alignment:Splign:2.1.0"
     exon            409..570
                     /gene="Tjap1"
                     /gene_synonym="0610041D19Rik; Pilt; Tjp4"
                     /inference="alignment:Splign:2.1.0"
     exon            571..637
                     /gene="Tjap1"
                     /gene_synonym="0610041D19Rik; Pilt; Tjp4"
                     /inference="alignment:Splign:2.1.0"
     exon            638..745
                     /gene="Tjap1"
                     /gene_synonym="0610041D19Rik; Pilt; Tjp4"
                     /inference="alignment:Splign:2.1.0"
     exon            746..829
                     /gene="Tjap1"
                     /gene_synonym="0610041D19Rik; Pilt; Tjp4"
                     /inference="alignment:Splign:2.1.0"
     exon            830..2462
                     /gene="Tjap1"
                     /gene_synonym="0610041D19Rik; Pilt; Tjp4"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2417..2422
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Tjap1"
                     /gene_synonym="0610041D19Rik; Pilt; Tjp4"
                     /note="hexamer: AATAAA"
     polyA_site      2439
                     /gene="Tjap1"
                     /gene_synonym="0610041D19Rik; Pilt; Tjp4"
                     /note="major polyA site"
ORIGIN      
        1 ggtccaggcc cctacccggc ctgccccgcg gcaagatggc tgcctgaagg agaggccccg
       61 gcagaaactt gaagacccaa gggccccagg gactggaaga aatcacattg cctgcttgga
      121 aagaagaggt agcaacttgc cccagcccaa ccccagagcg ggagctcttg gctagaggaa
      181 gtagaggggc cgtgggatgt ggagagccga aggctgattc caggagagca gaacagagag
      241 ctgccagcga acagacggag acgagcccat accttccagg atgagcagtg ctgcccctgc
      301 taagaaacct taccgtaagg cgcctccaga gcaccgagag ctacgcctgg agattcccgt
      361 gtcccggctc gagcaagagg agtccctgac tgacgcagag cggatgaaac ttttgcagca
      421 ggagaatgaa gaacttcgca aacgcctggc ctcagctacc agacgcactg aggccttgga
      481 gcgggagctg gaaataggac aagactgcct agaactggag ttaggacaga gccgtgagga
      541 gctggacaag tttaaggaca aattccgcag gctacagaac agctacacgg catcccagcg
      601 gaccaaccag gaactggagg acaagctgca cgcactgatc aagaaggctg agatggacag
      661 gaagacgctg gactgggaga ttgtggagct gaccaacaag ctactggatg ccaggaacac
      721 catcaataaa ctggaagagc tcaatgagcg gtaccggctg gactgcaacc tggcggtgca
      781 gcttctcaag tgcaacaagt cccacttccg gaaccacaag cttgctgacc taccctgtga
      841 gctccaggac atggttcgaa aacatctgcg cagtgggcag gaggttgcca gccccagccc
      901 cagcccttct tccagcctgt ccccaggggc tgtggtgcct acctcagtca ttgcccgggt
      961 gttggagaaa cctgagtctc tcttgctcaa ttcagctcag tctggcagtg ctgggcgccc
     1021 cttggctgag gatgtattcg tgcatgtgga catgagtggg ggtgacccag ccagcccccc
     1081 tgccccgggc agccccaatg gggagtgttg ctcagtgagt actgcagggg gctccccgga
     1141 ggaggagctg cccctgccag ccttcgacaa gttgagtccc taccctaccc cgtctccacc
     1201 acacccatta taccctggta ggaaggtgat agaattctct gaggacaaga tccgaattcc
     1261 tcggaacagc cccctcccca actgtaccta tgccacccgg caggccatct ccttgagcct
     1321 ggtggaggac ggcagtgagc gggcccaccg cagctcagtg cctagcagtc ctgcctctgc
     1381 ccaagggtcg ccccatcacc agcctagtcc agctccctca gcactgagtg ccccagctag
     1441 ttcggccagc tcggaggagg acctgctggc cagctggcag cgtgcatttg tggaccgcac
     1501 cccgccccct gctgccgtgg tccagcgcac ggcctttgga cgagactcac tgcccgagct
     1561 acaattgcat ttctccccgg gccactccac agccccaccg ccttccccgc accgtgagcg
     1621 gggacttgtg ctgccagcag aacctgactc tggctttcct caagacgagg aagaggaaat
     1681 gctgaacctg cctgtcagcc ccgaggaaga gcgtcagagc ctgctgcctg ataaagaggg
     1741 caccgaggag gcctctggcc cttctcatgt tgatggcagg gcctggcctc tccccagtcc
     1801 cagccgcccc cagcgcagcc ccaagagaat gggagtacac catctccacc gcaaggacag
     1861 cctgacgcag gcacaggagc agggcactgt gctcagctag acctgcctgc cttctgccac
     1921 cacctcaggg ctgcagatgg cccagactct ccctacagac ctgggcagct cagccagtgg
     1981 ggcccacatc aatccttgga gctgcgtttg cactaatggg tgccagcttg ccatcatcat
     2041 ttttgtttcc ttttcctttg aaatatttat tctccaaggt aacaagtcat ttgggcccca
     2101 agctcttcca ccatccagtc cgtccgtaag ggacgggcgg tgggcttgtc agttttcctt
     2161 tcccagctcc tttctcattg agagctctgg ttggtagggg agggtgtcgt tggtgctgct
     2221 gacctctatt cctctgcctc tgcgtgggct gctcttaggc ctgctcagct ggccccagag
     2281 acaagggtgg actcactcag ggctccccct tccagaggga gggcgtgact tctggggcct
     2341 ggccccattc ttaaccttgg ctctagactg ttactcccag atttgggaag ttttcacaat
     2401 gactatttta aaatcaaata aaacttattt tacaggtaag gcttaatttc tttctccttc
     2461 cc
//
