LOCUS       NM_080639               2944 bp    mRNA    linear   ROD 01-MAY-2024
DEFINITION  Mus musculus tissue inhibitor of metalloproteinase 4 (Timp4),
            transcript variant 1, mRNA.
ACCESSION   NM_080639
VERSION     NM_080639.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2944)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 2944)
  AUTHORS   Thurstin,A.A., Egeli,A.N., Goldsmith,E.C., Spinale,F.G. and
            LaVoie,H.A.
  TITLE     Tissue inhibitor of metalloproteinase-4 deletion in mice impacts
            maternal cardiac function during pregnancy and postpartum
  JOURNAL   Am J Physiol Heart Circ Physiol 324 (1), H85-H99 (2023)
   PUBMED   36459450
  REMARK    GeneRIF: Tissue inhibitor of metalloproteinase-4 deletion in mice
            impacts maternal cardiac function during pregnancy and postpartum.
REFERENCE   3  (bases 1 to 2944)
  AUTHORS   Hu,M., Jana,S., Kilic,T., Wang,F., Shen,M., Winkelaar,G.,
            Oudit,G.Y., Rayner,K., Zhang,D.W. and Kassiri,Z.
  TITLE     Loss of TIMP4 (Tissue Inhibitor of Metalloproteinase 4) Promotes
            Atherosclerotic Plaque Deposition in the Abdominal Aorta Despite
            Suppressed Plasma Cholesterol Levels
  JOURNAL   Arterioscler Thromb Vasc Biol 41 (6), 1874-1889 (2021)
   PUBMED   33792349
  REMARK    GeneRIF: Loss of TIMP4 (Tissue Inhibitor of Metalloproteinase 4)
            Promotes Atherosclerotic Plaque Deposition in the Abdominal Aorta
            Despite Suppressed Plasma Cholesterol Levels.
REFERENCE   4  (bases 1 to 2944)
  AUTHORS   Yonebayashi,S., Tajiri,K., Hara,M., Saito,H., Suzuki,N., Sakai,S.,
            Kimura,T., Sato,A., Sekimoto,A., Fujita,S., Okamoto,R.,
            Schwartz,R.J., Yoshida,T. and Imanaka-Yoshida,K.
  TITLE     Generation of Transgenic Mice that Conditionally Overexpress
            Tenascin-C
  JOURNAL   Front Immunol 12, 620541 (2021)
   PUBMED   33763067
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2944)
  AUTHORS   Solga,R., Behrens,J., Ziemann,A., Riou,A., Berwanger,C., Becker,L.,
            Garrett,L., de Angelis,M.H., Fischer,L., Coras,R., Barkovits,K.,
            Marcus,K., Mahabir,E., Eichinger,L., Schroder,R., Noegel,A.A. and
            Clemen,C.S.
  CONSRTM   German Mouse Clinic Consortium
  TITLE     CRN2 binds to TIMP4 and MMP14 and promotes perivascular invasion of
            glioblastoma cells
  JOURNAL   Eur J Cell Biol 98 (5-8), 151046 (2019)
   PUBMED   31677819
  REMARK    GeneRIF: The CRN2 increased the secretion of TIMP4 as well as the
            catalytic activity of MMP14.
REFERENCE   6  (bases 1 to 2944)
  AUTHORS   Thomas,G.T., Lewis,M.P. and Speight,P.M.
  TITLE     Matrix metalloproteinases and oral cancer
  JOURNAL   Oral Oncol 35 (3), 227-233 (1999)
   PUBMED   10621841
  REMARK    Review article
REFERENCE   7  (bases 1 to 2944)
  AUTHORS   Stetler-Stevenson,W.G.
  TITLE     Matrix metalloproteinases in angiogenesis: a moving target for
            therapeutic intervention
  JOURNAL   J Clin Invest 103 (9), 1237-1241 (1999)
   PUBMED   10225966
  REMARK    Review article
REFERENCE   8  (bases 1 to 2944)
  AUTHORS   Olson,T.M., Hirohata,S., Ye,J., Leco,K., Seldin,M.F. and Apte,S.S.
  TITLE     Cloning of the human tissue inhibitor of metalloproteinase-4 gene
            (TIMP4) and localization of the TIMP4 and Timp4 genes to human
            chromosome 3p25 and mouse chromosome 6, respectively
  JOURNAL   Genomics 51 (1), 148-151 (1998)
   PUBMED   9693046
REFERENCE   9  (bases 1 to 2944)
  AUTHORS   Leco,K.J., Apte,S.S., Taniguchi,G.T., Hawkes,S.P., Khokha,R.,
            Schultz,G.A. and Edwards,D.R.
  TITLE     Murine tissue inhibitor of metalloproteinases-4 (Timp-4): cDNA
            isolation and expression in adult mouse tissues
  JOURNAL   FEBS Lett 401 (2-3), 213-217 (1997)
   PUBMED   9013889
REFERENCE   10 (bases 1 to 2944)
  AUTHORS   Greene,J., Wang,M., Liu,Y.E., Raymond,L.A., Rosen,C. and Shi,Y.E.
  TITLE     Molecular cloning and characterization of human tissue inhibitor of
            metalloproteinase 4
  JOURNAL   J Biol Chem 271 (48), 30375-30380 (1996)
   PUBMED   8939999
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC153907.1.
            
            On Oct 13, 2017 this sequence version replaced NM_080639.3.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK140367.1, BC064046.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849382, SAMN01164141
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-566               AC153907.1         100260-100825       c
            567-664             AC153907.1         99000-99097         c
            665-779             AC153907.1         98449-98563         c
            780-904             AC153907.1         95850-95974         c
            905-2944            AC153907.1         93064-95103         c
FEATURES             Location/Qualifiers
     source          1..2944
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="6"
                     /map="6 53.29 cM"
     gene            1..2944
                     /gene="Timp4"
                     /gene_synonym="Timp-4"
                     /note="tissue inhibitor of metalloproteinase 4"
                     /db_xref="GeneID:110595"
                     /db_xref="MGI:MGI:109125"
     exon            1..566
                     /gene="Timp4"
                     /gene_synonym="Timp-4"
                     /inference="alignment:Splign:2.1.0"
     CDS             428..1102
                     /gene="Timp4"
                     /gene_synonym="Timp-4"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1; metalloproteinase inhibitor 4; tissue inhibitor
                     of metalloproteinases 4"
                     /codon_start=1
                     /product="metalloproteinase inhibitor 4 isoform 1
                     precursor"
                     /protein_id="NP_542370.3"
                     /db_xref="CCDS:CCDS20438.1"
                     /db_xref="GeneID:110595"
                     /db_xref="MGI:MGI:109125"
                     /translation="MPWSPLAALSWALVLRLLALLWPPGRGEACSCAPAHPQQHFCHS
                     ALVIRAKISSEKVVPASKDPADTQKLIRYEIKQIKMFKGFEKAKDIQYVYTPFDSSLC
                     GVKLETNSHKQYLLTGQILSDGKVFIHLCNYIEPWEDLSLVQRESLNHHYHQNCGCQI
                     TTCYAVPCTISAPNECLWTDWLLERKLYGYQAQHYVCMKHVDGICSWYRGHLHLRKEY
                     VDIIQP"
     exon            567..664
                     /gene="Timp4"
                     /gene_synonym="Timp-4"
                     /inference="alignment:Splign:2.1.0"
     exon            665..779
                     /gene="Timp4"
                     /gene_synonym="Timp-4"
                     /inference="alignment:Splign:2.1.0"
     exon            780..904
                     /gene="Timp4"
                     /gene_synonym="Timp-4"
                     /inference="alignment:Splign:2.1.0"
     exon            905..2944
                     /gene="Timp4"
                     /gene_synonym="Timp-4"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1461..1466
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Timp4"
                     /gene_synonym="Timp-4"
                     /note="hexamer: CATAAA"
     polyA_site      1480
                     /gene="Timp4"
                     /gene_synonym="Timp-4"
                     /note="major polyA site"
     regulatory      1550..1555
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Timp4"
                     /gene_synonym="Timp-4"
                     /note="hexamer: ACTAAA"
     polyA_site      1570
                     /gene="Timp4"
                     /gene_synonym="Timp-4"
                     /note="major polyA site"
     regulatory      1750..1755
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Timp4"
                     /gene_synonym="Timp-4"
                     /note="hexamer: AACAAG"
     polyA_site      1775
                     /gene="Timp4"
                     /gene_synonym="Timp-4"
ORIGIN      
        1 tgcctctgac ttggcctgtg tctctctgtg tctatgcttc ccttcactag ctcttgctct
       61 tagtgctctc cctcatctct ctgccttttc taagtctctt cactggtctt tgcttttttt
      121 tttttttttt tttttatctc tctgccccta actgcagaat cttcctctac ttccaggctc
      181 ttttcggaat ctgcaggttt ccctctgcaa cttcccaact gggtttgttg gtctggtcag
      241 gagggccttg ggtcctgtgg ctgtgggtgg cctggtgcca aaggagggag gagcccatgg
      301 gggcgtgatt ggctctaaag ccacctcata aagactgggg ctgcacgaag ctttctggag
      361 gagtcctgga ggggctgttt gttacgacag gcagacccca cgagctcagc tggggctctg
      421 cagtgtcatg ccctggagtc ccctggctgc gctgagctgg gcgctggtgc tgaggctgct
      481 ggctttgctg tggccccctg gacggggtga ggcgtgcagc tgtgcgcctg cgcaccccca
      541 gcagcacttc tgccactcgg ctctagtgat acgggccaaa atatccagtg agaaggtagt
      601 ccctgccagc aaagaccctg ctgacactca aaaactgatc cggtatgaaa tcaaacagat
      661 aaagatgttt aaagggttcg agaaggccaa ggatattcag tatgtctaca cgccatttga
      721 ctcttccctc tgtggtgtga agctagaaac caacagtcac aagcagtatc ttttgactgg
      781 ccagattctc agtgatggaa aagtcttcat ccatctgtgc aactacattg agccctggga
      841 ggacctgtcc ttggtgcaga gggagagcct gaatcatcac taccaccaga actgtggctg
      901 ccaaatcacc acttgctatg cagtgccatg taccatctca gcccccaatg agtgtctctg
      961 gacggactgg ctgctggaac ggaagctcta tgggtaccag gcccagcact atgtctgcat
     1021 gaagcatgtt gatggcatct gcagctggta ccgaggccac ctacacctcc ggaaggagta
     1081 cgttgacatc atccagccct agtagggacc aggaaccaca tccttggaag cattctgaag
     1141 accaagccag ttctctgtgg tcctttgacc atcaccacct gctgtgttgc tgccagataa
     1201 tggaaaatga caagtggatg gcctggccag caaatggagg ctgtggggca tgtgacagct
     1261 tgtgtctgct gttcacatct ccagctcaca acttgtcaag ggttggaaag gtatcttatc
     1321 tgccttgccc tcagcccctc ttccctgtct cccaaaccct gttagtctag cttataccta
     1381 ttactgagag tttttattct ggctttgttt aactggcttt tcttagctca tatgataagg
     1441 gagaagctgt gactgactgt cataaagttg tacaaccttg cgatgtgtgc tatggtaggt
     1501 gggctgactg tatcctgagt gggctgtttg gctgaagtga aaagaacaca ctaaagctcc
     1561 agatctccta ctcccactgt ctcaacttag cctaattctc tgacacactt ttatctgctt
     1621 gaaggacaca gtgggagctg aacagccagc ccgctcttcc tcttgtcctg caagtcccct
     1681 gatacttttg ctgacagaag gtctggccca gttggcatac atctctggaa aagaaaacgt
     1741 acatactaga acaagcattt tccctccagg ttaaatttct ctgttgcctt ctgttggtgt
     1801 ctggggactt ctcctttagg ttgctaatgt ctcttttcct actttagaac ttccttttta
     1861 aacaaacaaa caaacaaaaa aggtgaaaca acaaaggtgc cctcgtcacg atcttcctca
     1921 tcgtgatctt ttcctgaggc caagtttcag tagcagctga gagggagata atacaagatg
     1981 ctagtgtgga atacaccttt cacttgcttc aagcttcttc aagctgctag gtgttacagc
     2041 aggagaaagc cagctgtgac cccagagtgt ttctaccctc cagctcagtc cccttatcac
     2101 tgtgccaggc ccttgaaggc tcctcagcac ctctgaaagt cttcctggtt tgtctacatg
     2161 aaaaaaacac tagcaatagc ctctctggtt tattaagttg tagagctcaa tgtccccaaa
     2221 accttttcct gggaagatgg tagagattat cagttctttt tgacagctca ggaccagttt
     2281 tcatttttga tgtgtggtca actggtaagc agggccatct gtgctgtgct gggagaagga
     2341 gctggattta aatacaacga tggagatctc ttttggagga agaagtggga ggggaccaac
     2401 tatgatctct gtcccagaac acagcacttg gaaggctctg gcagtgagac tccaagtggg
     2461 gtcatccagt aaaaatctac accatgcttg ggcagggggt ggggatggag aggaagatgc
     2521 aaagtaacct ccaggttgct caatatgaaa catcctcaca gccttcaatc aaccatttat
     2581 cttttccttc gaacttctgg gggtggacac agaggagtaa taggattaac tctgccttgt
     2641 caccatttgg aggtggccaa agaaatacaa gcctagtgaa gaatgacaga agcaagagct
     2701 tttatctcag aggttttaca cctaccagaa ggctattacc tgaggaatct ctttggaatt
     2761 gtgtgtacac tgtattagat atcctggtca agggcagcta tgctcacttg gcatggttgg
     2821 gaaattgaca tgtggtgagg gaatgtaaca tgcccagtga cacatggcaa agctagagct
     2881 acactcaaaa ataatgaaac tccagactgc tggctcacag atttgttact ctctcacatt
     2941 aaaa
//
