LOCUS       NM_025616               1529 bp    mRNA    linear   ROD 14-FEB-2025
DEFINITION  Mus musculus translocase of inner mitochondrial membrane 50
            (Timm50), transcript variant 1, mRNA; nuclear gene for
            mitochondrial product.
ACCESSION   NM_025616
VERSION     NM_025616.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1529)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 1529)
  AUTHORS   Bedogni,F. and Hevner,R.F.
  TITLE     Cell-Type-Specific Gene Expression in Developing Mouse Neocortex:
            Intermediate Progenitors Implicated in Axon Development
  JOURNAL   Front Mol Neurosci 14, 686034 (2021)
   PUBMED   34321999
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 1529)
  AUTHORS   Tang,K., Zhao,Y., Li,H., Zhu,M., Li,W., Liu,W., Zhu,G., Xu,D.,
            Peng,W. and Xu,Y.W.
  TITLE     Translocase of Inner Membrane 50 Functions as a Novel Protective
            Regulator of Pathological Cardiac Hypertrophy
  JOURNAL   J Am Heart Assoc 6 (4), e004346 (2017)
   PUBMED   28432072
  REMARK    GeneRIF: TIM50 deficiency increased heart and cardiomyocyte size
            with more severe cardiac fibrosis compared with wild-type
            littermates. In cardiomyocyte-specific TIM50 transgenic mice, the
            hypertrophic and fibrotic phenotypes were all alleviated.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 1529)
  AUTHORS   Dickinson,M.E., Flenniken,A.M., Ji,X., Teboul,L., Wong,M.D.,
            White,J.K., Meehan,T.F., Weninger,W.J., Westerberg,H., Adissu,H.,
            Baker,C.N., Bower,L., Brown,J.M., Caddle,L.B., Chiani,F., Clary,D.,
            Cleak,J., Daly,M.J., Denegre,J.M., Doe,B., Dolan,M.E., Edie,S.M.,
            Fuchs,H., Gailus-Durner,V., Galli,A., Gambadoro,A., Gallegos,J.,
            Guo,S., Horner,N.R., Hsu,C.W., Johnson,S.J., Kalaga,S., Keith,L.C.,
            Lanoue,L., Lawson,T.N., Lek,M., Mark,M., Marschall,S., Mason,J.,
            McElwee,M.L., Newbigging,S., Nutter,L.M., Peterson,K.A.,
            Ramirez-Solis,R., Rowland,D.J., Ryder,E., Samocha,K.E.,
            Seavitt,J.R., Selloum,M., Szoke-Kovacs,Z., Tamura,M., Trainor,A.G.,
            Tudose,I., Wakana,S., Warren,J., Wendling,O., West,D.B., Wong,L.,
            Yoshiki,A., MacArthur,D.G., Tocchini-Valentini,G.P., Gao,X.,
            Flicek,P., Bradley,A., Skarnes,W.C., Justice,M.J., Parkinson,H.E.,
            Moore,M., Wells,S., Braun,R.E., Svenson,K.L., de Angelis,M.H.,
            Herault,Y., Mohun,T., Mallon,A.M., Henkelman,R.M., Brown,S.D.,
            Adams,D.J., Lloyd,K.C., McKerlie,C., Beaudet,A.L., Bucan,M. and
            Murray,S.A.
  CONSRTM   International Mouse Phenotyping Consortium; Jackson Laboratory;
            Infrastructure Nationale PHENOMIN, Institut Clinique de la Souris
            (ICS); Charles River Laboratories; MRC Harwell; Toronto Centre for
            Phenogenomics; Wellcome Trust Sanger Institute; RIKEN BioResource
            Center
  TITLE     High-throughput discovery of novel developmental phenotypes
  JOURNAL   Nature 537 (7621), 508-514 (2016)
   PUBMED   27626380
  REMARK    Erratum:[Nature. 2017 Nov 16;551(7680):398. doi:
            10.1038/nature24643.. PMID: 29144450]
REFERENCE   5  (bases 1 to 1529)
  AUTHORS   Guo,Y., Cheong,N., Zhang,Z., De Rose,R., Deng,Y., Farber,S.A.,
            Fernandes-Alnemri,T. and Alnemri,E.S.
  TITLE     Tim50, a component of the mitochondrial translocator, regulates
            mitochondrial integrity and cell death
  JOURNAL   J Biol Chem 279 (23), 24813-24825 (2004)
   PUBMED   15044455
REFERENCE   6  (bases 1 to 1529)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC148986.5.
            
            On Jan 24, 2023 this sequence version replaced NM_025616.3.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC010303.1, AK010832.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164138, SAMN01164139
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: reported by MitoCarta
            RefSeq Select criteria             :: based on single
                                                  protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-168               AC148986.5         205231-205398       c
            169-319             AC148986.5         204175-204325       c
            320-351             AC148986.5         203602-203633       c
            352-373             AC148986.5         203385-203406       c
            374-432             AC148986.5         201660-201718       c
            433-552             AC148986.5         201454-201573       c
            553-657             AC148986.5         201051-201155       c
            658-756             AC148986.5         200859-200957       c
            757-913             AC148986.5         200128-200284       c
            914-1020            AC148986.5         199729-199835       c
            1021-1529           AC148986.5         198828-199336       c
FEATURES             Location/Qualifiers
     source          1..1529
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="7"
                     /map="7 16.67 cM"
     gene            1..1529
                     /gene="Timm50"
                     /gene_synonym="2810403L02Rik; TIM50L"
                     /note="translocase of inner mitochondrial membrane 50"
                     /db_xref="GeneID:66525"
                     /db_xref="MGI:MGI:1913775"
     exon            1..168
                     /gene="Timm50"
                     /gene_synonym="2810403L02Rik; TIM50L"
                     /inference="alignment:Splign:2.1.0"
     CDS             61..1122
                     /gene="Timm50"
                     /gene_synonym="2810403L02Rik; TIM50L"
                     /note="mitochondrial import inner membrane translocase
                     subunit TIM50; translocase of inner mitochondrial membrane
                     50 homolog"
                     /codon_start=1
                     /product="mitochondrial import inner membrane translocase
                     subunit TIM50 precursor"
                     /protein_id="NP_079892.1"
                     /db_xref="CCDS:CCDS21039.1"
                     /db_xref="GeneID:66525"
                     /db_xref="MGI:MGI:1913775"
                     /translation="MAASAALFSRLRSGLRVGARGLCTRLAPPPPRTPEQVTEIANRG
                     GSKAQGPQHQPGSEGPSYAKKIALWIAGLLGAGGTVSIVYIFGNNPVDENGTKIPDEF
                     DSDPILVQQLRRTYKYFKDYRQMIIEPTSPCLLPDPLREPYYQPPYTLVLELTGVLLH
                     PEWSLATGWRFKKRPGIETLFQQLAPLYEIVIFTSETGMTAFPLIDSVDPHGFISYRL
                     FRDATRYMEGHHVKDISCLNRDPARVVVVDCKKEAFRLQPFNGVALRPWDGNSDDRVL
                     LDLSAFLKTIALNQVEDVRTVLEHYALEDDPLEAFKQRQSRLEQEEQQRLAELSKSNR
                     QGLSFGSLASRLWPRSKQP"
     exon            169..319
                     /gene="Timm50"
                     /gene_synonym="2810403L02Rik; TIM50L"
                     /inference="alignment:Splign:2.1.0"
     exon            320..351
                     /gene="Timm50"
                     /gene_synonym="2810403L02Rik; TIM50L"
                     /inference="alignment:Splign:2.1.0"
     exon            352..373
                     /gene="Timm50"
                     /gene_synonym="2810403L02Rik; TIM50L"
                     /inference="alignment:Splign:2.1.0"
     exon            374..432
                     /gene="Timm50"
                     /gene_synonym="2810403L02Rik; TIM50L"
                     /inference="alignment:Splign:2.1.0"
     exon            433..552
                     /gene="Timm50"
                     /gene_synonym="2810403L02Rik; TIM50L"
                     /inference="alignment:Splign:2.1.0"
     exon            553..657
                     /gene="Timm50"
                     /gene_synonym="2810403L02Rik; TIM50L"
                     /inference="alignment:Splign:2.1.0"
     exon            658..756
                     /gene="Timm50"
                     /gene_synonym="2810403L02Rik; TIM50L"
                     /inference="alignment:Splign:2.1.0"
     exon            757..913
                     /gene="Timm50"
                     /gene_synonym="2810403L02Rik; TIM50L"
                     /inference="alignment:Splign:2.1.0"
     exon            914..1020
                     /gene="Timm50"
                     /gene_synonym="2810403L02Rik; TIM50L"
                     /inference="alignment:Splign:2.1.0"
     exon            1021..1529
                     /gene="Timm50"
                     /gene_synonym="2810403L02Rik; TIM50L"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1204..1209
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Timm50"
                     /gene_synonym="2810403L02Rik; TIM50L"
                     /note="hexamer: AATAAA"
     polyA_site      1229
                     /gene="Timm50"
                     /gene_synonym="2810403L02Rik; TIM50L"
                     /note="major polyA site"
ORIGIN      
        1 gtcctcgcct gggcgggaga gagggagcga gtgggcgggg ccgcgtggcg tcagcgcaag
       61 atggcggcct cggcggctct gttctcgcgc ttgcggagcg ggctccgggt aggcgcgcgg
      121 gggctgtgca cgcggctagc gccgccacct ccccgcactc ccgagcaggt cactgaaatt
      181 gccaaccgtg ggggctccaa ggcccagggg ccacagcacc agccaggctc cgagggtccc
      241 agctatgcca aaaaaattgc gctctggatc gccggtttgc tcggagctgg tgggactgtc
      301 agcatcgtct atatttttgg aaacaaccct gtggatgaaa atggtaccaa gattcctgat
      361 gaattcgaca gcgatccaat tctggttcag cagttgcgcc ggacatacaa atacttcaaa
      421 gattacagac agatgatcat cgagcccacc agtccctgcc ttctcccaga ccctctgcgg
      481 gagccctact accagccacc ttatacactg gtcctggagc ttaccggggt ccttctgcac
      541 ccagagtggt cgctggccac tggctggagg tttaagaagc gtccaggcat cgagaccttg
      601 ttccagcagc ttgcccctct gtatgaaata gtcatcttca catcagagac tggcatgact
      661 gcatttccac tcattgatag tgtggaccct cacggtttca tctcctaccg tctgttccgg
      721 gacgccacca gatatatgga gggacatcat gtgaaggaca tctcctgtct gaatcgggac
      781 ccagcccgag tagtagtcgt ggactgcaag aaggaagctt tccgcctcca gcccttcaac
      841 ggtgttgccc tgcggccctg ggatggcaac tcagatgacc gggtcttgct ggacctgtct
      901 gccttcctca agaccattgc actgaaccaa gtggaggacg tccggacggt gctggagcac
      961 tatgctctag aggatgaccc actggaggca ttcaaacagc ggcagagccg gcttgagcag
     1021 gaggagcagc agcgcctggc cgagctctcc aagtccaaca ggcagggcct ctcctttggc
     1081 tcactcgcca gccgactgtg gcctcgctcc aagcagccct gacttctagg cttcctcaga
     1141 gacttgatgc ctgagccttg ggccccagcc cttacacggc aaagtgtgga cagctatgtg
     1201 tccaataaag cgagtcccag atgccacaat gcctgtgtcc agagtccaga tggggcactg
     1261 gggtgaggtc ttggactcgg tcgtcattca cagtgactga gcaccaccac agggctgggt
     1321 gccatgagtg cctcgttgga gtggggctcc tgccagcctg aaactgaaaa cctgggtttc
     1381 tgctgcacat gtcttcccat atactctatg agttggcaaa gaaaagcccc tctctctctc
     1441 tccctccctc tttctctggc tctggactcc ttgtgtgctg tagaaataaa gagaagtctg
     1501 gcctctgggg agccgcctcg agggctgtc
//
