LOCUS       NM_019818               2838 bp    mRNA    linear   ROD 09-FEB-2025
DEFINITION  Mus musculus translocase of inner mitochondrial membrane 22
            (Timm22), transcript variant 1, mRNA; nuclear gene for
            mitochondrial product.
ACCESSION   NM_019818
VERSION     NM_019818.5
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2838)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 2838)
  AUTHORS   Cheong,A., Archambault,D., Degani,R., Iverson,E., Tremblay,K.D. and
            Mager,J.
  TITLE     Nuclear-encoded mitochondrial ribosomal proteins are required to
            initiate gastrulation
  JOURNAL   Development 147 (10) (2020)
   PUBMED   32376682
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2838)
  AUTHORS   Sundberg,J.P., Dadras,S.S., Silva,K.A., Kennedy,V.E., Garland,G.,
            Murray,S.A., Sundberg,B.A., Schofield,P.N. and Pratt,C.H.
  TITLE     Systematic screening for skin, hair, and nail abnormalities in a
            large-scale knockout mouse program
  JOURNAL   PLoS One 12 (7), e0180682 (2017)
   PUBMED   28700664
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2838)
  AUTHORS   Dickinson,M.E., Flenniken,A.M., Ji,X., Teboul,L., Wong,M.D.,
            White,J.K., Meehan,T.F., Weninger,W.J., Westerberg,H., Adissu,H.,
            Baker,C.N., Bower,L., Brown,J.M., Caddle,L.B., Chiani,F., Clary,D.,
            Cleak,J., Daly,M.J., Denegre,J.M., Doe,B., Dolan,M.E., Edie,S.M.,
            Fuchs,H., Gailus-Durner,V., Galli,A., Gambadoro,A., Gallegos,J.,
            Guo,S., Horner,N.R., Hsu,C.W., Johnson,S.J., Kalaga,S., Keith,L.C.,
            Lanoue,L., Lawson,T.N., Lek,M., Mark,M., Marschall,S., Mason,J.,
            McElwee,M.L., Newbigging,S., Nutter,L.M., Peterson,K.A.,
            Ramirez-Solis,R., Rowland,D.J., Ryder,E., Samocha,K.E.,
            Seavitt,J.R., Selloum,M., Szoke-Kovacs,Z., Tamura,M., Trainor,A.G.,
            Tudose,I., Wakana,S., Warren,J., Wendling,O., West,D.B., Wong,L.,
            Yoshiki,A., MacArthur,D.G., Tocchini-Valentini,G.P., Gao,X.,
            Flicek,P., Bradley,A., Skarnes,W.C., Justice,M.J., Parkinson,H.E.,
            Moore,M., Wells,S., Braun,R.E., Svenson,K.L., de Angelis,M.H.,
            Herault,Y., Mohun,T., Mallon,A.M., Henkelman,R.M., Brown,S.D.,
            Adams,D.J., Lloyd,K.C., McKerlie,C., Beaudet,A.L., Bucan,M. and
            Murray,S.A.
  CONSRTM   International Mouse Phenotyping Consortium; Jackson Laboratory;
            Infrastructure Nationale PHENOMIN, Institut Clinique de la Souris
            (ICS); Charles River Laboratories; MRC Harwell; Toronto Centre for
            Phenogenomics; Wellcome Trust Sanger Institute; RIKEN BioResource
            Center
  TITLE     High-throughput discovery of novel developmental phenotypes
  JOURNAL   Nature 537 (7621), 508-514 (2016)
   PUBMED   27626380
  REMARK    Erratum:[Nature. 2017 Nov 16;551(7680):398. doi:
            10.1038/nature24643.. PMID: 29144450]
REFERENCE   5  (bases 1 to 2838)
  AUTHORS   Poche,R.A., Zhang,M., Rueda,E.M., Tong,X., McElwee,M.L., Wong,L.,
            Hsu,C.W., Dejosez,M., Burns,A.R., Fox,D.A., Martin,J.F., Zwaka,T.P.
            and Dickinson,M.E.
  TITLE     RONIN Is an Essential Transcriptional Regulator of Genes Required
            for Mitochondrial Function in the Developing Retina
  JOURNAL   Cell Rep 14 (7), 1684-1697 (2016)
   PUBMED   26876175
REFERENCE   6  (bases 1 to 2838)
  AUTHORS   Koscielny,G., Yaikhom,G., Iyer,V., Meehan,T.F., Morgan,H.,
            Atienza-Herrero,J., Blake,A., Chen,C.K., Easty,R., Di Fenza,A.,
            Fiegel,T., Grifiths,M., Horne,A., Karp,N.A., Kurbatova,N.,
            Mason,J.C., Matthews,P., Oakley,D.J., Qazi,A., Regnart,J.,
            Retha,A., Santos,L.A., Sneddon,D.J., Warren,J., Westerberg,H.,
            Wilson,R.J., Melvin,D.G., Smedley,D., Brown,S.D., Flicek,P.,
            Skarnes,W.C., Mallon,A.M. and Parkinson,H.
  TITLE     The International Mouse Phenotyping Consortium Web Portal, a
            unified point of access for knockout mice and related phenotyping
            data
  JOURNAL   Nucleic Acids Res 42 (Database issue), D802-D809 (2014)
   PUBMED   24194600
REFERENCE   7  (bases 1 to 2838)
  AUTHORS   Sharov,A.A., Piao,Y., Matoba,R., Dudekula,D.B., Qian,Y.,
            VanBuren,V., Falco,G., Martin,P.R., Stagg,C.A., Bassey,U.C.,
            Wang,Y., Carter,M.G., Hamatani,T., Aiba,K., Akutsu,H., Sharova,L.,
            Tanaka,T.S., Kimber,W.L., Yoshikawa,T., Jaradat,S.A., Pantano,S.,
            Nagaraja,R., Boheler,K.R., Taub,D., Hodes,R.J., Longo,D.L.,
            Schlessinger,D., Keller,J., Klotz,E., Kelsoe,G., Umezawa,A.,
            Vescovi,A.L., Rossant,J., Kunath,T., Hogan,B.L., Curci,A.,
            D'Urso,M., Kelso,J., Hide,W. and Ko,M.S.
  TITLE     Transcriptome analysis of mouse stem cells and early embryos
  JOURNAL   PLoS Biol 1 (3), E74 (2003)
   PUBMED   14691545
REFERENCE   8  (bases 1 to 2838)
  AUTHORS   Suzuki,H., Fukunishi,Y., Kagawa,I., Saito,R., Oda,H., Endo,T.,
            Kondo,S., Bono,H., Okazaki,Y. and Hayashizaki,Y.
  TITLE     Protein-protein interaction panel using mouse full-length cDNAs
  JOURNAL   Genome Res 11 (10), 1758-1765 (2001)
   PUBMED   11591653
REFERENCE   9  (bases 1 to 2838)
  AUTHORS   Bauer,M.F., Rothbauer,U., Muhlenbein,N., Smith,R.J., Gerbitz,K.,
            Neupert,W., Brunner,M. and Hofmann,S.
  TITLE     The mitochondrial TIM22 preprotein translocase is highly conserved
            throughout the eukaryotic kingdom
  JOURNAL   FEBS Lett 464 (1-2), 41-47 (1999)
   PUBMED   10611480
REFERENCE   10 (bases 1 to 2838)
  AUTHORS   Voss,G.C. and Jockusch,H.
  TITLE     New testis-specific expressed genes on mouse Chromosome 11
  JOURNAL   Mamm Genome 7 (2), 163 (1996)
   PUBMED   8835539
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BY309364.1, AK012130.2 and BB724262.1.
            
            On Mar 27, 2014 this sequence version replaced NM_019818.4.
            
            Transcript Variant: This variant (1) represents the longest
            transcript and it encodes the longest isoform (1).
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK153837.1, AK012130.2 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: reported by MitoCarta
            RefSeq Select criteria             :: based on conservation,
                                                  expression, longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-47                BY309364.1         2-48
            48-2817             AK012130.2         2-2771
            2818-2838           BB724262.1         199-219
FEATURES             Location/Qualifiers
     source          1..2838
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="11"
                     /map="11 45.92 cM"
     gene            1..2838
                     /gene="Timm22"
                     /gene_synonym="Tim22"
                     /note="translocase of inner mitochondrial membrane 22"
                     /db_xref="GeneID:56322"
                     /db_xref="MGI:MGI:1929742"
     exon            1..300
                     /gene="Timm22"
                     /gene_synonym="Tim22"
                     /inference="alignment:Splign:2.1.0"
     CDS             63..647
                     /gene="Timm22"
                     /gene_synonym="Tim22"
                     /note="isoform 1 is encoded by transcript variant 1;
                     preprotein translocase; mitochondrial import inner
                     membrane translocase subunit Tim22; translocase of inner
                     mitochondrial membrane 22 homolog"
                     /codon_start=1
                     /product="mitochondrial import inner membrane translocase
                     subunit Tim22 isoform 1"
                     /protein_id="NP_062792.2"
                     /db_xref="CCDS:CCDS25065.1"
                     /db_xref="GeneID:56322"
                     /db_xref="MGI:MGI:1929742"
                     /translation="MAATAPKAGGSAPEAAGSAEAPLQYSLLLQYLVGDKRQPRLLEP
                     GSLGGIPSPAKSEEQKMIERAMESCAFKAVLACVGGFVLGGAFGIFTAGIDTNVGFDP
                     KDPYRTPTAKEVLKDMGQRGMSYAKNFAIVGAMFSCTECLVESYRGKSDWKNSVISGC
                     ITGGAIGFRAGVKAGAIGCGGFAAFSAAIDYYLR"
     exon            301..497
                     /gene="Timm22"
                     /gene_synonym="Tim22"
                     /inference="alignment:Splign:2.1.0"
     exon            498..570
                     /gene="Timm22"
                     /gene_synonym="Tim22"
                     /inference="alignment:Splign:2.1.0"
     exon            571..2838
                     /gene="Timm22"
                     /gene_synonym="Tim22"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1066..1071
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Timm22"
                     /gene_synonym="Tim22"
                     /note="hexamer: AGTAAA"
     polyA_site      1097
                     /gene="Timm22"
                     /gene_synonym="Tim22"
                     /note="major polyA site"
     regulatory      2223..2228
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Timm22"
                     /gene_synonym="Tim22"
                     /note="hexamer: AATAAT"
     polyA_site      2247
                     /gene="Timm22"
                     /gene_synonym="Tim22"
     regulatory      2798..2803
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Timm22"
                     /gene_synonym="Tim22"
                     /note="hexamer: AATAAA"
     polyA_site      2817
                     /gene="Timm22"
                     /gene_synonym="Tim22"
ORIGIN      
        1 ggtaagcgtg tggaatcggc tgtgggaaat acgaagtgaa aggtactaga gcccctacgg
       61 tcatggcggc taccgccccc aaggccgggg gttcagctcc tgaggcagcg ggttctgccg
      121 aagctccgtt gcagtacagc cttcttcttc agtacctggt gggcgacaag cgtcagcccc
      181 ggcttctgga gcctggcagc ctgggcggga tcccgagtcc tgccaagagc gaggagcaga
      241 agatgatcga gagggcaatg gaaagttgcg ccttcaaggc tgtgctggcc tgtgtaggag
      301 ggtttgtctt gggaggcgcg tttggtatct ttactgctgg cattgatacc aacgtaggct
      361 ttgacccaaa ggacccttac cggacaccaa ctgcaaaaga agtcctgaaa gacatgggac
      421 agagaggaat gtcctatgcc aaaaactttg ccattgtggg cgccatgttt tcatgtactg
      481 agtgtctggt agagtcttac cggggaaagt cggactggaa gaacagcgtc atcagtggct
      541 gcatcactgg cggagccatc ggcttccgag ctggagtaaa ggccggggcc ataggttgtg
      601 gagggtttgc tgctttctct gctgcaatcg attattacct acggtgaaag aaacagccgg
      661 gaaggaaaga ggacgccagc caccttcaga gctgctctgt ggagcagttt ctacacactt
      721 ggactgcttc aggggtgaag ccaagtgcat ccctctacag ttgatggtgt gaaggacaca
      781 cctcgctgct cgatcgctct ctgcagccat tggaacaacc atacttttcc acttgaattc
      841 caaccaggtt tttcagggga ggtggtatgg cagagtgtct ccttgcagta aacagatcag
      901 gaggcagtgt gtggactgtt acgcattaag ctttgaacca ggctcagttc tgacctggct
      961 cagctcccca gctttgaata gggaagtggc agtgtttgcc catagccggg aagctcttct
     1021 tccttctgtg tgcaagtatc catcatgagt cccagggcag ggcaaagtaa acttatgcca
     1081 tcgaggcaat tatgtgattt cagttagtat ctttgctgtc cttgttaaaa aatatggggg
     1141 ttggggattt agctcagtgg taaagtgctt gcctagcaag cccaaggccc tgggttcggt
     1201 cctcagctcc ggaaaaagga aaaaaaaaat atatgtatat gggggctgtg agaaggctca
     1261 gtagttaata ccagaggtcc caggtttggt tcccagcacc tacattgtgg gttacaacca
     1321 tctgtaactc tagttccaga gatctaaggt tctcttctgg cctttgaggg cacctgagtg
     1381 gtgcagacat atacacatgc aggcaaaaat acccaatttt atttttttta aagcagatgt
     1441 actattatta ggagctagtt tttcagattt cacacctctg acaaaagatg ccagctggcc
     1501 caaagagtga caaatccaat ggaaaggcta ggaaacctct aaggaactct gggagcgttg
     1561 ctcttagcgt ctgtggaagc tgtatctttg ttaccacagc cactccatca gcaaggcctc
     1621 tccctagaaa gtttagccat gttcctggat gtattgcaac atgctggtaa ctcctctgcc
     1681 tcctcaaagg cattggattg gctgatggtc atgcctacag cttacaaaac ccttggtctt
     1741 gggccccaac ccaggtgaaa ggtccgtgaa tgtcttactg tggtcaccct gccagctgag
     1801 tcagtcatac tgtagctact ggcccagatc agatggcaga ggcctgttca ccaccactcc
     1861 tgttctccgc taagcttttc tttggctttt ggtggtttgt tttttgttac tgttattcaa
     1921 cagttcagcc taattatacc atggcagaga acgagccttt tatgtttggg ctgtgccact
     1981 gaactgttta ctgtagcgtg tgggtgaagg tggaactaat gggctcagtc cttacctcct
     2041 gcttctgtgt aggaggctca gccgaggctt ggaactggct accttcagcc agcagtcttt
     2101 tcccctgctg tatagcaacc cttctaccct tgcttttctt gcttcctcat cttcactcaa
     2161 ccttaagcag agttcaaaga ctcaacttca acattggtca tctgggtgtg tatttatatg
     2221 tgaataatga tatcagatcc agagtaacac ctttgctgtc ttcttaggat gggtgagtgc
     2281 acggggctcg ggctctttgc tgaatacttt tcgttcctat gccgaggaaa gaatccaagt
     2341 caggtactca attagtcaat ctccctgctt ctaatctgca gtgaattctg tatgatgcct
     2401 gccctatttc ataagcacag agcttcagtc gtgcctaccc cagagctctg gaaacaggct
     2461 taggagtgct tcctgtattg tctgtaaatc cttcacctag gaacactgat gcaaggatgg
     2521 actctacctg gtacctgtca aagtgctcag gtgcctgagg tcttttacca gctgctcttt
     2581 cacatgagcg ccagtacaag aaatgctgcc atcacagcta ccttggcaca gaggggcctc
     2641 agctgtcagt agccatcagt tgaacaagcc aagccttgtc cacgtggacc attcaatcaa
     2701 aaggagatga atagcttcct gttttagatg gctgggggtc agtatgagct cataaaccaa
     2761 acagcagttt gcagacacat ctgttcctga tctctagaat aaacagtgtc caatggcttc
     2821 agctgattaa gtcccaca
//
