LOCUS       NM_001122998            6144 bp    mRNA    linear   ROD 30-JUL-2024
DEFINITION  Mus musculus T cell lymphoma invasion and metastasis 2 (Tiam2),
            transcript variant 2, mRNA.
ACCESSION   NM_001122998
VERSION     NM_001122998.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 6144)
  AUTHORS   Rao,S., Liang,F. and Herring,B.E.
  TITLE     RhoGEF Tiam2 Regulates Glutamatergic Synaptic Transmission in
            Hippocampal CA1 Pyramidal Neurons
  JOURNAL   eNeuro 11 (7) (2024)
   PUBMED   38871458
  REMARK    GeneRIF: RhoGEF Tiam2 Regulates Glutamatergic Synaptic Transmission
            in Hippocampal CA1 Pyramidal Neurons.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6144)
  AUTHORS   Chu,C.H., Chen,J.S., Chan,Y.L., Lu,W.J., Huang,Y.T., Mao,P.C.,
            Sze,C.I. and Sun,H.S.
  TITLE     TIAM2S-positive microglia enhance inflammation and neurotoxicity
            through soluble ICAM-1-mediated immune priming
  JOURNAL   FASEB J 37 (11), e23242 (2023)
   PUBMED   37801065
  REMARK    GeneRIF: TIAM2S-positive microglia enhance inflammation and
            neurotoxicity through soluble ICAM-1-mediated immune priming.
REFERENCE   3  (bases 1 to 6144)
  AUTHORS   Bedogni,F. and Hevner,R.F.
  TITLE     Cell-Type-Specific Gene Expression in Developing Mouse Neocortex:
            Intermediate Progenitors Implicated in Axon Development
  JOURNAL   Front Mol Neurosci 14, 686034 (2021)
   PUBMED   34321999
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 6144)
  AUTHORS   Chu,C.H., Chen,J.S., Chuang,P.C., Su,C.H., Chan,Y.L., Yang,Y.J.,
            Chiang,Y.T., Su,Y.Y., Gean,P.W. and Sun,H.S.
  TITLE     TIAM2S as a novel regulator for serotonin level enhances brain
            plasticity and locomotion behavior
  JOURNAL   FASEB J 34 (2), 3267-3288 (2020)
   PUBMED   31908036
  REMARK    GeneRIF: TIAM2S as a novel regulator for serotonin level enhances
            brain plasticity and locomotion behavior.
REFERENCE   5  (bases 1 to 6144)
  AUTHORS   Charron,Y., Willert,J., Lipkowitz,B., Kusecek,B., Herrmann,B.G. and
            Bauer,H.
  TITLE     Two isoforms of the RAC-specific guanine nucleotide exchange factor
            TIAM2 act oppositely on transmission ratio distortion by the mouse
            t-haplotype
  JOURNAL   PLoS Genet 15 (2), e1007964 (2019)
   PUBMED   30817801
  REMARK    GeneRIF: Tiam2 is expressed in two isoforms, the full-length
            (Tiam2l) and a short transcript (Tiam2s). Tiam2s expression from
            the t-allele is strongly increased compared to the wild-type
            allele. By transgenic approaches we show that Tiam2s enhances
            t-haplotype transmission, while Tiam2l has the opposite effect.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 6144)
  AUTHORS   Yoshizawa,M., Hoshino,M., Sone,M. and Nabeshima,Y.
  TITLE     Expression of stef, an activator of Rac1, correlates with the
            stages of neuronal morphological development in the mouse brain
  JOURNAL   Mech Dev 113 (1), 65-68 (2002)
   PUBMED   11900975
REFERENCE   7  (bases 1 to 6144)
  AUTHORS   Matsuo,N., Hoshino,M., Yoshizawa,M. and Nabeshima,Y.
  TITLE     Characterization of STEF, a guanine nucleotide exchange factor for
            Rac1, required for neurite growth
  JOURNAL   J Biol Chem 277 (4), 2860-2868 (2002)
   PUBMED   11707441
REFERENCE   8  (bases 1 to 6144)
  AUTHORS   Piao,Y., Ko,N.T., Lim,M.K. and Ko,M.S.
  TITLE     Construction of long-transcript enriched cDNA libraries from
            submicrogram amounts of total RNAs by a universal PCR amplification
            method
  JOURNAL   Genome Res 11 (9), 1553-1558 (2001)
   PUBMED   11544199
REFERENCE   9  (bases 1 to 6144)
  AUTHORS   Hoshino,M., Sone,M., Fukata,M., Kuroda,S., Kaibuchi,K.,
            Nabeshima,Y. and Hama,C.
  TITLE     Identification of the stef gene that encodes a novel guanine
            nucleotide exchange factor specific for Rac1
  JOURNAL   J Biol Chem 274 (25), 17837-17844 (1999)
   PUBMED   10364228
REFERENCE   10 (bases 1 to 6144)
  AUTHORS   Kawasaki,H., Springett,G.M., Toki,S., Canales,J.J., Harlan,P.,
            Blumenstiel,J.P., Chen,E.J., Bany,I.A., Mochizuki,N., Ashbacher,A.,
            Matsuda,M., Housman,D.E. and Graybiel,A.M.
  TITLE     A Rap guanine nucleotide exchange factor enriched highly in the
            basal ganglia
  JOURNAL   Proc Natl Acad Sci U S A 95 (22), 13278-13283 (1998)
   PUBMED   9789079
  REMARK    Erratum:[Proc Natl Acad Sci U S A 1999 Jan 5;96(1):318]
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BC067048.1, BC079600.1, AK129474.1 and BB084682.1.
            
            Transcript Variant: This variant (2) has an alternate 5' UTR exon,
            compared to variant 1. Variants 1 and 2 encode the same isoform
            (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC067048.1, BC079600.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849380
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-111               BC067048.1         1-111
            112-5730            BC079600.1         1-5619
            5731-6132           AK129474.1         5634-6035
            6133-6144           BB084682.1         309-320
FEATURES             Location/Qualifiers
     source          1..6144
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="17"
                     /map="17 1.99 cM"
     gene            1..6144
                     /gene="Tiam2"
                     /gene_synonym="3000002F19Rik; mKIAA2016; STEF"
                     /note="T cell lymphoma invasion and metastasis 2"
                     /db_xref="GeneID:24001"
                     /db_xref="MGI:MGI:1344338"
     exon            1..154
                     /gene="Tiam2"
                     /gene_synonym="3000002F19Rik; mKIAA2016; STEF"
                     /inference="alignment:Splign:2.1.0"
     exon            155..273
                     /gene="Tiam2"
                     /gene_synonym="3000002F19Rik; mKIAA2016; STEF"
                     /inference="alignment:Splign:2.1.0"
     exon            274..1514
                     /gene="Tiam2"
                     /gene_synonym="3000002F19Rik; mKIAA2016; STEF"
                     /inference="alignment:Splign:2.1.0"
     CDS             327..5474
                     /gene="Tiam2"
                     /gene_synonym="3000002F19Rik; mKIAA2016; STEF"
                     /note="isoform 1 is encoded by transcript variant 2;
                     RAS-related C3 botulinum substrate 1, guanine nucleotide
                     exchange factor 1; T-lymphoma invasion and
                     metastasis-inducing protein 2; TIAM-2; SIF and TIAM1-like
                     exchange factor; T-cell lymphoma invasion and metastasis
                     2; RAS-related C3 botulinum substrate 1, guanine
                     nucleotide exchange factor 1; rho guanine nucleotide
                     exchange factor TIAM2"
                     /codon_start=1
                     /product="rho guanine nucleotide exchange factor TIAM2
                     isoform 1"
                     /protein_id="NP_001116470.1"
                     /db_xref="CCDS:CCDS37421.1"
                     /db_xref="GeneID:24001"
                     /db_xref="MGI:MGI:1344338"
                     /translation="MGNSESQYTFQGSKNHSNTVTGAKQKPCSLKIRSVHAKDEKSLH
                     GWTHGSSGAGYKSRSLARSCLSHFKNHQPYATRLSGPTCKVSKGTTYSKHRANTPGND
                     FQGNSGAFLPENGFHYVDRESEESHITSNGHLLTCYGRKESLASTPPGEDHRSPRVLI
                     KTLGKLDGCLRVEFHNGGNPHKGTSEDPSGPVRLLRYSPTLASETCPVRETRRHSAAG
                     SPSSQRPSPTDSRLRSSKGSSLSSESSWYDSPWGNAGEVSEVEGSFLAPSTPDPSLPS
                     SFPPSDTKKPFNQSSSLSSLRELYKDPNLGCRSPSGTCLSSNEYISSQVSLNNRVSFA
                     SDMDVPSRVDHRDPLHYSSFTLPCRKSKALTEDAAKKDTLKARMRRFSDWTGSLSRKK
                     RKLQEPRSMEGSEYFDSHSDGLNAEGQVPAQTSSLLWSGGSAQTLPHRSESTHAISVD
                     PLRQNIYENFMRELEMSRSNTEHVETSTETMESSSESVSSLEQLDLLFEKEQGVVRKA
                     GWLFFKPLVTLQKERKLELVARRKWKQYWVTLKGCTLLFYETYGKNSTEQNSAPRCAL
                     FAEDSIVQSVPEHPKKEHVFCLSNSCGDVYLFQATSQTDLENWVTAIHSACASLFAKK
                     HGKEDTVRLLKSQTRSLLQKIDMDSKMKKMAELQLSVVSDPKNRKAIENQIRQWEQNL
                     EKFHMDLFRMRCYLASLQGGELPNPKSLLAATSRPSKLALGRLGVLSVSSFHALVCSR
                     DDSTLRKRTLSLTQRGKSKKGIFSSLKGLDTLARKGREKRASITQMFDSSHSHGFLGT
                     QLPQKSTNSNKAHDLHLYGSAVDSALRDSMWEVQTYVHFQDNEGVTVTIKPEHRVEDV
                     LALVCKMRQLEPTHYGLQLRKVVDKSVEWCVPALYEYMQEQVYDEIEVFPLSVYDVQL
                     TKTGDMTDFGFAVTVQVDEHQHLNRIFISDVLPDSLAYGGGLRKGNEITSLNGEPVSD
                     LDIQQMEALFSEKSVGLTLVARPVTTRRTLCASWSDSDLFSRDQKSLPPSPNQSQLLE
                     EFLDNFRKTATSDFSNVPEITTGLKRSQTEGTLDQVPHREKMEQTFLSADQIAELCRD
                     LNNTHTNSMEAPTESHDPPPRPLARHLSDADRLRKVIQELVDTEKSYVKDLSCLFELY
                     LEPLQNETFLTQDEMESLFGSLPEMLEFQKVFLETLEDAISASSDFSVLETPSQFRKL
                     LFSLGGSFLYYADHFKLYSGFCANHIKVQRVLERAKTDKAFKAFLDARNPTKQHSSTL
                     ESYLIKPVQRVLKYPLLLKELVSLTDHESEEHYHLTEALKAMEKVASHINEMQKIYED
                     YGMVFDQLVAEQSGTEKEVTELSMGELLMHSTVSWLNPFLSLGKARKDIELTVFVFKR
                     AVILVYKENCKLKKKLPSNSRPAHNSADLDPFKFRWLIPISALQVRLGNTAGTENNST
                     WELIHTKSEIEGRPETIFQLCCSDSENKTSIVKVIRSILRENFRRHIKCELPLEKTCK
                     DRLVPLKNRVPVSAKLASSRSLKGLRTSSSSEWPSEPSKGNSLDSDECSLSSGTQSSG
                     CPVAESRRDSKSTELEKDAQEGLAEFPDGLIKESDILSDEDEDFHHPLKQGSPTKDIE
                     IQFQRLKISEESDVHPVGQQPLTESGEQPKLVRGHFCPIKRKANSTKRGRGTLLKAQT
                     RHQSLDSHPETASIDLNLVLEREFSVQSLTSVVNEEGFYETQSHGKS"
     exon            1515..1950
                     /gene="Tiam2"
                     /gene_synonym="3000002F19Rik; mKIAA2016; STEF"
                     /inference="alignment:Splign:2.1.0"
     exon            1951..2123
                     /gene="Tiam2"
                     /gene_synonym="3000002F19Rik; mKIAA2016; STEF"
                     /inference="alignment:Splign:2.1.0"
     exon            2124..2348
                     /gene="Tiam2"
                     /gene_synonym="3000002F19Rik; mKIAA2016; STEF"
                     /inference="alignment:Splign:2.1.0"
     exon            2349..2534
                     /gene="Tiam2"
                     /gene_synonym="3000002F19Rik; mKIAA2016; STEF"
                     /inference="alignment:Splign:2.1.0"
     exon            2535..2681
                     /gene="Tiam2"
                     /gene_synonym="3000002F19Rik; mKIAA2016; STEF"
                     /inference="alignment:Splign:2.1.0"
     exon            2682..2753
                     /gene="Tiam2"
                     /gene_synonym="3000002F19Rik; mKIAA2016; STEF"
                     /inference="alignment:Splign:2.1.0"
     exon            2754..2912
                     /gene="Tiam2"
                     /gene_synonym="3000002F19Rik; mKIAA2016; STEF"
                     /inference="alignment:Splign:2.1.0"
     exon            2913..3017
                     /gene="Tiam2"
                     /gene_synonym="3000002F19Rik; mKIAA2016; STEF"
                     /inference="alignment:Splign:2.1.0"
     exon            3018..3096
                     /gene="Tiam2"
                     /gene_synonym="3000002F19Rik; mKIAA2016; STEF"
                     /inference="alignment:Splign:2.1.0"
     exon            3097..3189
                     /gene="Tiam2"
                     /gene_synonym="3000002F19Rik; mKIAA2016; STEF"
                     /inference="alignment:Splign:2.1.0"
     exon            3190..3453
                     /gene="Tiam2"
                     /gene_synonym="3000002F19Rik; mKIAA2016; STEF"
                     /inference="alignment:Splign:2.1.0"
     exon            3454..3557
                     /gene="Tiam2"
                     /gene_synonym="3000002F19Rik; mKIAA2016; STEF"
                     /inference="alignment:Splign:2.1.0"
     exon            3558..3737
                     /gene="Tiam2"
                     /gene_synonym="3000002F19Rik; mKIAA2016; STEF"
                     /inference="alignment:Splign:2.1.0"
     exon            3738..3806
                     /gene="Tiam2"
                     /gene_synonym="3000002F19Rik; mKIAA2016; STEF"
                     /inference="alignment:Splign:2.1.0"
     exon            3807..3932
                     /gene="Tiam2"
                     /gene_synonym="3000002F19Rik; mKIAA2016; STEF"
                     /inference="alignment:Splign:2.1.0"
     exon            3933..4041
                     /gene="Tiam2"
                     /gene_synonym="3000002F19Rik; mKIAA2016; STEF"
                     /inference="alignment:Splign:2.1.0"
     exon            4042..4221
                     /gene="Tiam2"
                     /gene_synonym="3000002F19Rik; mKIAA2016; STEF"
                     /inference="alignment:Splign:2.1.0"
     exon            4222..4340
                     /gene="Tiam2"
                     /gene_synonym="3000002F19Rik; mKIAA2016; STEF"
                     /inference="alignment:Splign:2.1.0"
     exon            4341..4449
                     /gene="Tiam2"
                     /gene_synonym="3000002F19Rik; mKIAA2016; STEF"
                     /inference="alignment:Splign:2.1.0"
     exon            4450..4508
                     /gene="Tiam2"
                     /gene_synonym="3000002F19Rik; mKIAA2016; STEF"
                     /inference="alignment:Splign:2.1.0"
     exon            4509..4614
                     /gene="Tiam2"
                     /gene_synonym="3000002F19Rik; mKIAA2016; STEF"
                     /inference="alignment:Splign:2.1.0"
     exon            4615..4702
                     /gene="Tiam2"
                     /gene_synonym="3000002F19Rik; mKIAA2016; STEF"
                     /inference="alignment:Splign:2.1.0"
     exon            4703..4857
                     /gene="Tiam2"
                     /gene_synonym="3000002F19Rik; mKIAA2016; STEF"
                     /inference="alignment:Splign:2.1.0"
     exon            4858..6143
                     /gene="Tiam2"
                     /gene_synonym="3000002F19Rik; mKIAA2016; STEF"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5705..5710
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Tiam2"
                     /gene_synonym="3000002F19Rik; mKIAA2016; STEF"
                     /note="hexamer: AATAAA"
     polyA_site      5725
                     /gene="Tiam2"
                     /gene_synonym="3000002F19Rik; mKIAA2016; STEF"
                     /note="major polyA site"
     regulatory      6121..6126
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Tiam2"
                     /gene_synonym="3000002F19Rik; mKIAA2016; STEF"
                     /note="hexamer: ATTAAA"
     polyA_site      6143
                     /gene="Tiam2"
                     /gene_synonym="3000002F19Rik; mKIAA2016; STEF"
                     /note="major polyA site"
ORIGIN      
        1 cggagctaga gaagccttga ccttcccggc tcagcgagtg gagaacaaag tgacggagaa
       61 aacttcgcgg agtgtgccct gagtccccac ggggcctcgc tcaggggcgc agagccgggc
      121 tcgaccggtc ctctgtgttg aaacttggac cgaggatcac ttcatggatt tgcctcacgt
      181 acctatgttt gttaatttcc tgtgtcgttc ccaagatcac gtaaaaccct ctgagcatta
      241 ttgacctccc tcccagcaaa agccaggctg cagtgtaatt tagaccatgg ctcatattga
      301 tacaactgtt ctcaattttg gttaaaatgg ggaactccga gagtcaatat accttccaag
      361 gatccaagaa tcatagtaat actgtcactg gtgctaagca aaagccttgc tctctgaaaa
      421 tacgcagcgt tcatgcaaaa gacgagaagt ccttacatgg ttggactcat gggagcagcg
      481 gtgcaggcta caagtccagg tccctagccc gaagctgcct ttctcacttt aagaatcacc
      541 agccttacgc caccagactc agtggaccca catgtaaagt ctcaaagggc accacctact
      601 ctaagcacag agcaaatacc ccgggaaatg atttccaggg caacagtggt gctttcttac
      661 ctgagaatgg cttccactat gttgaccgcg agtcagagga aagccatatc acctccaatg
      721 ggcaccttct cacctgctat gggagaaagg aaagcctcgc ctccactcct ccaggcgagg
      781 accacaggag ccccagggtg ctcatcaaga ccctgggaaa gctagacggg tgtttaagag
      841 tcgagttcca caacggtggc aacccccaca aggggacctc cgaggacccc agtggacctg
      901 tacggctgct gagatactcc cctaccttag catcggaaac ctgcccggtg cgggaaacca
      961 ggcggcattc cgctgcaggc tccccatcca gccagcggcc ctctcccact gactctcgcc
     1021 tgcgctccag caaaggcagc tccctgagct cagagtcatc ctggtatgac tccccctggg
     1081 gcaacgctgg ggaggtgagc gaggtggagg gctccttcct ggctcccagc actccagacc
     1141 ccagcctccc cagcagcttc ccacccagtg acaccaaaaa gcctttcaac caaagctctt
     1201 ccctctcctc cctccgggaa ttgtacaaag atcccaacct ggggtgccgc tcaccttccg
     1261 gcacctgcct ttcttccaat gagtacatca gctctcaagt cagcctgaac aaccgagtct
     1321 cctttgcgtc tgacatggat gtgccctcca gggtggatca cagggatccc ctgcactata
     1381 gttcctttac tctcccctgt cgcaagtcca aagccttaac tgaagatgca gctaagaaag
     1441 acaccctcaa agccagaatg cggcgcttca gtgactggac aggaagcctc tccaggaaga
     1501 agaggaaact gcaggaaccc aggtccatgg agggcagtga gtactttgat agccactcag
     1561 atggactgaa tgcagaaggg caggtgcccg cgcagacatc ttccttactg tggtcagggg
     1621 gctcggctca gaccctgcct cacagaagcg aatccactca cgctatcagc gtcgatcccc
     1681 tccgacagaa catctatgag aatttcatgc gagagctcga aatgagcagg agcaacacag
     1741 aacacgtgga aacttccaca gagaccatgg agtccagcag cgagtctgtc agctcactgg
     1801 agcagctgga tctgctcttt gagaaggagc agggagtggt ccggaaagcc gggtggctct
     1861 tcttcaaacc ccttgtcacc ttgcagaagg agaggaaact ggagctggtg gctcggagga
     1921 agtggaaaca atactgggtg accctgaaag gctgtactct gctgttttat gagacctatg
     1981 gaaagaattc cacagagcag aatagtgccc cacggtgtgc cctctttgca gaggacagca
     2041 tcgtgcagtc tgtcccagag catcccaaga aggaacacgt gttctgcctg agtaactcct
     2101 gtggggacgt ctacctattc caggccacta gccagacaga tctggaaaac tgggtcacag
     2161 ccatccactc ggcgtgcgca tccctctttg caaagaagca cggaaaggag gacacggtgc
     2221 gactgctaaa gagccagacc agaagcctgc ttcagaagat agacatggat agcaagatga
     2281 agaagatggc agagttgcag ctgtctgtgg tgagcgaccc caagaacagg aaggccatcg
     2341 agaatcagat ccggcaatgg gagcagaatc tggaaaaatt ccacatggac ctgttccgca
     2401 tgcgctgcta tttggcgagc ttacaaggtg gggagttacc aaatcccaag agtctccttg
     2461 ctgccaccag ccgcccctcc aagctggctc ttggcaggct gggcgtcctg tctgtttcgt
     2521 ctttccatgc tctggtgtgt tccagagatg attccactct caggaaaaga acactttccc
     2581 ttacccagag aggaaaaagc aagaaaggca tattttcttc attgaaaggt ctggacaccc
     2641 tagcaagaaa gggtagggag aagagagctt ccataactca gatgtttgat tctagccaca
     2701 gccatggatt tcttggaact cagctacctc aaaagtccac taactccaac aaggcccatg
     2761 acctgcatct gtatggctcc gcagtagaca gcgcgctgcg agacagcatg tgggaagtcc
     2821 agacttatgt ccacttccag gataacgaag gagttactgt gaccatcaag ccagagcaca
     2881 gggtggaaga tgttctggct ttggtgtgca agatgagaca gttggaaccc actcactatg
     2941 gtcttcagct ccgaaaggtg gtcgataaaa gtgtggagtg gtgtgtgccc gcgctgtatg
     3001 aatacatgca agagcaggtt tatgatgaaa tcgaagtttt cccactcagt gtgtatgacg
     3061 tgcagctaac caagactggg gacatgactg actttgggtt tgcagtcaca gttcaggtgg
     3121 acgaacacca gcatctcaac cggatattta tcagtgatgt tctccctgac agcctggcat
     3181 acggaggagg gctgagaaag ggcaacgaaa tcacaagctt aaatggggaa ccagtgtctg
     3241 accttgacat ccagcagatg gaggctttgt tttctgagaa gagcgttggc ctcactctag
     3301 tcgcccggcc tgtgaccaca agacgaaccc tgtgtgcttc ctggtcagac agtgacctgt
     3361 tctccaggga ccagaaaagt ctgccgccct ctcccaacca gtcccagctg ctggaggaat
     3421 tcctggataa ctttagaaaa accgccacga gtgatttcag caatgtccct gagatcacaa
     3481 ctggcttgaa gaggagccag acagaaggca ccctggatca agtgccccac agggaaaaga
     3541 tggagcagac attcctgagc gctgaccaga ttgcggagct ctgcagggac ttgaacaaca
     3601 cccacaccaa cagtatggaa gcaccaacag agagccatga cccacctccc aggcctctgg
     3661 ctcgtcacct ctcagatgca gatcgcctcc ggaaagtcat ccaggagctt gtagacacag
     3721 agaagtctta cgtgaaggat ctgagctgcc tctttgaact atacttggag ccacttcaga
     3781 atgagacctt tcttacccaa gatgagatgg agtcactttt tgggagcctg ccagagatgc
     3841 tggagtttca aaaggtgttc ctggagacgt tggaggatgc gatctccgct tcctcggact
     3901 ttagtgtcct ggaaaccccc tcacagtttc gaaaattgct gttctccctt ggaggttctt
     3961 tcctctacta tgcggatcac tttaagctat acagtgggtt ctgtgccaac cacattaaag
     4021 tacagagggt tctagagcga gctaaaacgg acaaggcctt caaggctttt ctggatgccc
     4081 gaaatcccac caagcagcac tcctccacgc tggagtccta tctcatcaag cctgttcaga
     4141 gagtgctcaa gtatcctctg cttctcaagg agctagtgtc actgactgac catgagagtg
     4201 aagaacacta tcacctgaca gaagcactaa aggccatgga aaaagtcgcc agtcacatca
     4261 atgagatgca gaagatctac gaggattacg ggatggtgtt tgaccagctg gtggcagagc
     4321 agagtggcac agagaaggaa gtgacagagc tgtccatggg ggaacttctg atgcactcta
     4381 cagtttcctg gttgaatccg ttcctgtctc taggaaaagc caggaaggac attgagctca
     4441 cagtatttgt ttttaagaga gctgtcatac tggtttataa agaaaactgc aagctgaaaa
     4501 agaaactgcc ctcgaattcc cggcctgctc acaactctgc tgacttggat ccatttaaat
     4561 tccgctggtt gattcccata tctgcgcttc aagttagact ggggaacacg gcagggactg
     4621 aaaataattc cacgtgggag ctgattcata ccaagtcgga aattgaagga cggccagaaa
     4681 ccatctttca actgtgctgc agtgacagcg agaacaaaac cagcattgtt aaggtgattc
     4741 gttctattct gagagagaac ttccggcgcc acataaagtg tgagctgcca ctggagaaga
     4801 cgtgtaagga ccggctagta cctcttaaga accgagttcc tgtttcagcc aaattagcct
     4861 cgtccaggtc gttgaagggc ctcagaacat cctccagcag cgagtggccc agcgagccca
     4921 gcaagggcaa ctcactggac tcagatgagt gcagcctgag cagtggcacc cagagtagcg
     4981 gctgccccgt agccgagagc aggcgagact ctaagagcac cgagctggag aaagacgctc
     5041 aggagggcct ggcggagttt ccagatggtc ttatcaaaga aagcgacatt ctgagtgatg
     5101 aagatgagga cttccaccac cctctgaaac agggtagccc tactaaggac attgagattc
     5161 agttccagag actgaaaatc tctgaggaat ccgacgtgca cccagttggg cagcagcctc
     5221 tcacagagtc aggtgaacag cccaagctgg tcaggggcca tttttgcccc attaaacgga
     5281 aagcaaacag caccaagagg ggcagaggaa ctttgctcaa ggcgcagact cgtcaccagt
     5341 ccctggacag ccacccagaa actgccagca ttgatctaaa cttggtcctg gagagagaat
     5401 tcagtgtcca gagcttaact tcagtcgtca atgaggaggg tttttatgaa acacagagcc
     5461 atggcaaatc atgatttgtg tagtgtgctt acaatgctca tttgttttca aattggtgga
     5521 aaagagaaac tgcaggaaaa ctattcatgt ttgagtttta cgcagtatat tttttccaca
     5581 aaacctgtaa agatttaagt tattttaatt tattgtgaat ccgaaaacta gatttagttg
     5641 gtcagaatct gtaaagtact agcacctatc cctttggagc agcaatcaaa tgatttagtc
     5701 ttgtaataaa attgcatttt aattatctta ggtgcttttt ttttcttggt agcctcatcc
     5761 aggtccttga aggtcctcag aacatcctcc agcagtgagt ggcccagcga gcccagcaag
     5821 ggtgactcac tagactcagg atgtagcctg agcagtgtca cccagagtag cggctgccct
     5881 ggtagccgag agcaggcaag gctctaagag cactgtgctt gggaaagttg tgattaacag
     5941 cttttaagag ggcccctacc cccagtcctt tgggcatttt ctttgagctg ttaggttttg
     6001 ctttatttaa agcactttta tttttacatg gtttagaggc acaacgtaca gatatttcat
     6061 tttagaaggt tgtcataaat gctattttta ctaaacatgt cgtacctatg cagtatatac
     6121 attaaaggaa agcttgcact gtaa
//
