LOCUS       NM_001145886            7277 bp    mRNA    linear   ROD 24-JUN-2024
DEFINITION  Mus musculus T cell lymphoma invasion and metastasis 1 (Tiam1),
            transcript variant 2, mRNA.
ACCESSION   NM_001145886
VERSION     NM_001145886.1
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 7277)
  AUTHORS   Zhang,Y., Zhang,H., Zhao,S., Qi,Z., He,Y., Zhang,X., Wu,W., Yan,K.,
            Hu,L., Sun,S., Tang,X., Zhou,Q., Chen,F., Gu,A., Wang,L., Zhang,Z.,
            Yu,B., Wang,D., Han,Y., Xie,L. and Ji,Y.
  TITLE     S-Nitrosylation of Septin2 Exacerbates Aortic Aneurysm and
            Dissection by Coupling the TIAM1-RAC1 Axis in Macrophages
  JOURNAL   Circulation 149 (24), 1903-1920 (2024)
   PUBMED   38357802
  REMARK    GeneRIF: S-Nitrosylation of Septin2 Exacerbates Aortic Aneurysm and
            Dissection by Coupling the TIAM1-RAC1 Axis in Macrophages.
REFERENCE   2  (bases 1 to 7277)
  AUTHORS   Wu,Y., Liu,W., Li,J., Shi,H., Ma,S., Wang,D., Pan,B., Xiao,R.,
            Jiang,H. and Liu,X.
  TITLE     Decreased Tiam1-mediated Rac1 activation is responsible for
            impaired directional persistence of chondrocyte migration in
            microtia
  JOURNAL   J Cell Mol Med 28 (11), e18443 (2024)
   PUBMED   38837873
  REMARK    GeneRIF: Decreased Tiam1-mediated Rac1 activation is responsible
            for impaired directional persistence of chondrocyte migration in
            microtia.
REFERENCE   3  (bases 1 to 7277)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   4  (bases 1 to 7277)
  AUTHORS   Li,X., Liu,B., Wang,X., Zhuang,Y., Gao,T. and Sun,N.
  TITLE     Tiam1 regulates proliferation, invasion, and differentiation in
            neuroblastoma through the Tiam1/Rac1 signaling pathway
  JOURNAL   Neoplasma 71 (1), 37-47 (2024)
   PUBMED   38506031
  REMARK    GeneRIF: Tiam1 regulates proliferation, invasion, and
            differentiation in neuroblastoma through the Tiam1/Rac1 signaling
            pathway.
REFERENCE   5  (bases 1 to 7277)
  AUTHORS   Machin,P.A., Johnsson,A.E., Massey,E.J., Pantarelli,C.,
            Chetwynd,S.A., Chu,J.Y., Okkenhaug,H., Segonds-Pichon,A.,
            Walker,S., Malliri,A., Fukui,Y. and Welch,H.C.E.
  TITLE     Dock2 generates characteristic spatiotemporal patterns of Rac
            activity to regulate neutrophil polarisation, migration and
            phagocytosis
  JOURNAL   Front Immunol 14, 1180886 (2023)
   PUBMED   37383235
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 7277)
  AUTHORS   Wei,J., Hodes,M.E., Wang,Y., Feng,Y., Ghetti,B. and Dlouhy,S.R.
  TITLE     Direct cDNA selection with DNA microdissected from mouse chromosome
            16: isolation of novel clones and construction of a partial
            transcription map of the C3-C4 region
  JOURNAL   Genome Res 6 (8), 678-687 (1996)
   PUBMED   8858343
REFERENCE   7  (bases 1 to 7277)
  AUTHORS   Schmidt,T., Zornig,M., Beneke,R. and Moroy,T.
  TITLE     MoMuLV proviral integrations identified by Sup-F selection in
            tumors from infected myc/pim bitransgenic mice correlate with
            activation of the gfi-1 gene
  JOURNAL   Nucleic Acids Res 24 (13), 2528-2534 (1996)
   PUBMED   8692692
REFERENCE   8  (bases 1 to 7277)
  AUTHORS   Fan,C.M., Kuwana,E., Bulfone,A., Fletcher,C.F., Copeland,N.G.,
            Jenkins,N.A., Crews,S., Martinez,S., Puelles,L., Rubenstein,J.L.
            and Tessier-Lavigne,M.
  TITLE     Expression patterns of two murine homologs of Drosophila
            single-minded suggest possible roles in embryonic patterning and in
            the pathogenesis of Down syndrome
  JOURNAL   Mol Cell Neurosci 7 (1), 1-16 (1996)
   PUBMED   8812055
  REMARK    Erratum:[Mol Cell Neurosci 1996 Jun;7(6):519]
REFERENCE   9  (bases 1 to 7277)
  AUTHORS   Habets,G.G., van der Kammen,R.A., Jenkins,N.A., Gilbert,D.J.,
            Copeland,N.G., Hagemeijer,A. and Collard,J.G.
  TITLE     The invasion-inducing TIAM1 gene maps to human chromosome band
            21q22 and mouse chromosome 16
  JOURNAL   Cytogenet Cell Genet 70 (1-2), 48-51 (1995)
   PUBMED   7736788
REFERENCE   10 (bases 1 to 7277)
  AUTHORS   Habets,G.G., Scholtes,E.H., Zuydgeest,D., van der Kammen,R.A.,
            Stam,J.C., Berns,A. and Collard,J.G.
  TITLE     Identification of an invasion-inducing gene, Tiam-1, that encodes a
            protein with homology to GDP-GTP exchangers for Rho-like proteins
  JOURNAL   Cell 77 (4), 537-549 (1994)
   PUBMED   7999144
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK147392.1, AK017465.1, AK136856.1, CO042855.1, CJ262109.1 and
            BC065126.1.
            
            Transcript Variant: This variant (2) differs in the 5' UTR compared
            to variant 1. Both variants 1 and 2 encode the same isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK147392.1 [ECO:0000332]
            RNAseq introns              :: partial sample support SAMN00849374,
                                           SAMN00849375 [ECO:0000350]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-468               AK147392.1         2-469
            469-691             AK017465.1         420-642
            692-5961            AK147392.1         693-5962
            5962-6636           AK136856.1         2895-3569
            6637-6826           CO042855.1         448-637             c
            6827-7253           CJ262109.1         90-516
            7254-7277           BC065126.1         5202-5225
FEATURES             Location/Qualifiers
     source          1..7277
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="16"
                     /map="16 51.5 cM"
     gene            1..7277
                     /gene="Tiam1"
                     /gene_synonym="D16Ium10; D16Ium10e"
                     /note="T cell lymphoma invasion and metastasis 1"
                     /db_xref="GeneID:21844"
                     /db_xref="MGI:MGI:103306"
     exon            1..116
                     /gene="Tiam1"
                     /gene_synonym="D16Ium10; D16Ium10e"
                     /inference="alignment:Splign:2.1.0"
     exon            117..288
                     /gene="Tiam1"
                     /gene_synonym="D16Ium10; D16Ium10e"
                     /inference="alignment:Splign:2.1.0"
     exon            289..457
                     /gene="Tiam1"
                     /gene_synonym="D16Ium10; D16Ium10e"
                     /inference="alignment:Splign:2.1.0"
     exon            458..1431
                     /gene="Tiam1"
                     /gene_synonym="D16Ium10; D16Ium10e"
                     /inference="alignment:Splign:2.1.0"
     CDS             469..5244
                     /gene="Tiam1"
                     /gene_synonym="D16Ium10; D16Ium10e"
                     /note="isoform 1 is encoded by transcript variant 2;
                     T-lymphoma invasion and metastasis-inducing protein 1;
                     TIAM-1; rho guanine nucleotide exchange factor TIAM1"
                     /codon_start=1
                     /product="rho guanine nucleotide exchange factor TIAM1
                     isoform 1"
                     /protein_id="NP_001139358.1"
                     /db_xref="CCDS:CCDS28314.1"
                     /db_xref="GeneID:21844"
                     /db_xref="MGI:MGI:103306"
                     /translation="MGNAESQNVDHEFYGEKHASLGRKHTSRSLRLSHKTRRTRHASS
                     GKAIHRNSEVSTRSSSTPSIPQSLAENGLEPFSQEGALDDFGDPIWVDRVDMGLRPVS
                     YTDSSVTPSVDGSIVLTAASVQSMPDSEESRLYGDDATYLAEGGRRQCPYTSNGPTFM
                     ETASFKKKRSKSADIWREDSLEFSLSDLSQEHLTSNEEILGSAEEKDCEEARGMETEA
                     SPRQLSTCQRANSLGDLYAQKNSGVKANGGPRNRFSSYCRNLVSDIPDLAKHKMPPAA
                     AEETPPYSNYNTLPCRKSHCLSEGATNPQISLSKSMQGRRAKTTQDVNTGEGSEFADS
                     GIEGATTDTDLLSRRSNATNSSYSPPTGRAFVGSDSGSSSTGDAARQGVYENFRRELE
                     MSTTNSESLEEAGSAHSDEQSSGTLSSPGQSDILLTAAQGTVRKAGALAVKNFLVHKK
                     NKKVESATRRKWKHYWVSLKGCTLFFYETDGRSGIDHNSVPKHAVWVENSIVQAVPEH
                     PKKDFVFCLSNSLGDAFLFQTTSQTELENWITAIHSACAAAVARHHHKEDTLRLLKSE
                     IKKLEQKIDMDEKMKKMGEMQLSSVTDSKKKKTILDQIFVWEQNLEQFQMDLFRFRCY
                     LASLQGGELPNPKRLLAFASRPTKVAMGRLGIFSVSSFHALVAARTGEIGVRRRTQAM
                     SRSASKRRSRFSSLWGLDTTSKKKQGRPTINQVFGEGTDAVKRSLEGIFDDTVPDGKR
                     EKEVVLPSVHQHNPDCDIWVHEYFTPSWFCLPNNQPALTVVRPGDTARDTLELICKTH
                     QLDHSAHYLRLKFLMENRVQFYIPQPEEDIYELLYKEIEICPKVTQNIHIEKSDAAAD
                     NYGFLLSSVDEDGIRRLYVNSVKETGLASKKGLKAGDEILEINNRAAGTLNSSMLKDF
                     LSQPSLGLLVRTYPEPEGGVELLENPPHRVDGPVDLGESPLAFLTSNPGHSLSSEQGS
                     SAETAPEEGEGPDLESSDETDHSSKSTEQVAAFCRSLHEMSPSDSSPSPQDATSPQLA
                     TTRQLSDADKLRKVICELLETERTYVKDLNCLMERYLKPLQKETFLTQDELDVLFGNL
                     TEMVEFQVEFLKTLEDGVRLVPDLEKLEKVDQFKKVLFSLGGSFLYYADRFKLYSAFC
                     ASHTKVPKVLVKAKTDTAFKAFLDAQNPRQQHSSTLESYLIKPIQRVLKYPLLLRELF
                     ALTDAESEEHYHLDVAIKTMNKVASHINEMQKIHEEFGAVFDQLIAEQTGEKKEVADL
                     SMGDLLLHTSVIWLNPPASLGKWKKEPELAAFVFKTAVVLVYKDGSKQKKKLVGSHRL
                     SIYEEWDPFRFRHMIPTEALQVRALPSADAEANAVCEIVHVKSESEGRPERVFHLCCS
                     SPESRKDFLKSVHSILRDKHRRQLLKTESLPSAQQYVPFGGKRLCALKGARPAMSRAV
                     SAPSKSLGRRRRRLARNRFTIDSDAISASSPEKEPQQPAGGGDTDRWVEEQFDLAQYE
                     EQDDIKETDILSDDDEFCESLKGASVDRDLQEQLQAASISQRARGRRTLDSHASRMTQ
                     LKKQAALSGINGGLESASEEVIWVRREDFAPSRKLNTEI"
     exon            1432..1879
                     /gene="Tiam1"
                     /gene_synonym="D16Ium10; D16Ium10e"
                     /inference="alignment:Splign:2.1.0"
     exon            1880..2052
                     /gene="Tiam1"
                     /gene_synonym="D16Ium10; D16Ium10e"
                     /inference="alignment:Splign:2.1.0"
     exon            2053..2277
                     /gene="Tiam1"
                     /gene_synonym="D16Ium10; D16Ium10e"
                     /inference="alignment:Splign:2.1.0"
     exon            2278..2463
                     /gene="Tiam1"
                     /gene_synonym="D16Ium10; D16Ium10e"
                     /inference="alignment:Splign:2.1.0"
     exon            2464..2610
                     /gene="Tiam1"
                     /gene_synonym="D16Ium10; D16Ium10e"
                     /inference="alignment:Splign:2.1.0"
     exon            2611..2685
                     /gene="Tiam1"
                     /gene_synonym="D16Ium10; D16Ium10e"
                     /inference="alignment:Splign:2.1.0"
     exon            2686..2856
                     /gene="Tiam1"
                     /gene_synonym="D16Ium10; D16Ium10e"
                     /inference="alignment:Splign:2.1.0"
     exon            2857..2961
                     /gene="Tiam1"
                     /gene_synonym="D16Ium10; D16Ium10e"
                     /inference="alignment:Splign:2.1.0"
     exon            2962..3043
                     /gene="Tiam1"
                     /gene_synonym="D16Ium10; D16Ium10e"
                     /inference="alignment:Splign:2.1.0"
     exon            3044..3130
                     /gene="Tiam1"
                     /gene_synonym="D16Ium10; D16Ium10e"
                     /inference="alignment:Splign:2.1.0"
     exon            3131..3355
                     /gene="Tiam1"
                     /gene_synonym="D16Ium10; D16Ium10e"
                     /inference="alignment:Splign:2.1.0"
     exon            3356..3459
                     /gene="Tiam1"
                     /gene_synonym="D16Ium10; D16Ium10e"
                     /inference="alignment:Splign:2.1.0"
     exon            3460..3639
                     /gene="Tiam1"
                     /gene_synonym="D16Ium10; D16Ium10e"
                     /inference="alignment:Splign:2.1.0"
     exon            3640..3708
                     /gene="Tiam1"
                     /gene_synonym="D16Ium10; D16Ium10e"
                     /inference="alignment:Splign:2.1.0"
     exon            3709..3834
                     /gene="Tiam1"
                     /gene_synonym="D16Ium10; D16Ium10e"
                     /inference="alignment:Splign:2.1.0"
     exon            3835..3943
                     /gene="Tiam1"
                     /gene_synonym="D16Ium10; D16Ium10e"
                     /inference="alignment:Splign:2.1.0"
     exon            3944..4123
                     /gene="Tiam1"
                     /gene_synonym="D16Ium10; D16Ium10e"
                     /inference="alignment:Splign:2.1.0"
     exon            4124..4242
                     /gene="Tiam1"
                     /gene_synonym="D16Ium10; D16Ium10e"
                     /inference="alignment:Splign:2.1.0"
     exon            4243..4351
                     /gene="Tiam1"
                     /gene_synonym="D16Ium10; D16Ium10e"
                     /inference="alignment:Splign:2.1.0"
     exon            4352..4410
                     /gene="Tiam1"
                     /gene_synonym="D16Ium10; D16Ium10e"
                     /inference="alignment:Splign:2.1.0"
     exon            4411..4513
                     /gene="Tiam1"
                     /gene_synonym="D16Ium10; D16Ium10e"
                     /inference="alignment:Splign:2.1.0"
     exon            4514..4601
                     /gene="Tiam1"
                     /gene_synonym="D16Ium10; D16Ium10e"
                     /inference="alignment:Splign:2.1.0"
     exon            4602..4774
                     /gene="Tiam1"
                     /gene_synonym="D16Ium10; D16Ium10e"
                     /inference="alignment:Splign:2.1.0"
     exon            4775..7255
                     /gene="Tiam1"
                     /gene_synonym="D16Ium10; D16Ium10e"
                     /inference="alignment:Splign:2.1.0"
     regulatory      7236..7241
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Tiam1"
                     /gene_synonym="D16Ium10; D16Ium10e"
                     /note="hexamer: AATAAA"
     polyA_site      7255
                     /gene="Tiam1"
                     /gene_synonym="D16Ium10; D16Ium10e"
                     /note="major polyA site"
ORIGIN      
        1 atccttccta tttaggtgat gcttaagtgc tgcatatttc tcgtcctagt ccgtccctgt
       61 ccttgctgtt tctgtttccg actgaagtgg cttctgtggt tacaggagag acttgggtag
      121 aaccattgtg gactgaaacc cacctttcca gatctgaaag gaagactgag gaagaggaag
      181 ggaggggaca gtcccggaga caattctagt ccctcactca ggacttgagc agcgccttca
      241 gatggcacct ttggagatct tgaccaaggc tcttctgttt ggcctggggt ttctggcaca
      301 ttctcccaga ctcaggtgag accccgatgg gcaccccgtg gggcaggtcc tgacacacgt
      361 cccgactcca gccactgaca ccattgccag cctcagtctc taaatgccac accgctcctg
      421 gggccagttg ggctgggcac cactgttgcc tttgaagacc ataaaaccat gggaaacgca
      481 gaaagccaaa atgtagacca cgagttttat ggagaaaagc atgccagcct ggggcggaag
      541 cacacttcac gctccctgcg gctgtcacac aagacccgga ggacacggca tgcttcctcc
      601 gggaaggcga tccacaggaa ctctgaagtg agcacccggt ccagcagcac gcccagcatc
      661 ccccagtccc tggctgaaaa tggcctggag cccttctccc aagaaggcgc cctagacgac
      721 ttcggggacc ccatctgggt ggaccgagta gatatgggct tgagacccgt atcttacacc
      781 gattcttccg tcactcccag cgtagatggc agcatcgtcc tcactgcagc ctctgtgcag
      841 agcatgccag actcagagga gagccggctt tacggggatg acgctacgta cttggctgag
      901 ggaggcagga ggcagtgtcc ctatacatcc aatgggccca cattcatgga gacagcgagc
      961 tttaagaaga agcgctccaa atctgcagac atctggcggg aggacagcct ggaattctca
     1021 ctctcagatc tgagccaaga acatttaaca agcaacgaag aaatcttggg ttccgcggaa
     1081 gagaaggatt gcgaggaggc tcgggggatg gaaacagagg cgagtccccg gcagctcagc
     1141 acctgtcagc gagccaactc cctgggtgac ttgtatgctc agaaaaactc tggggtgaag
     1201 gctaatggag gaccgaggaa cagattctca agctactgcc ggaatttggt gtcggatatt
     1261 cccgatcttg caaagcataa gatgccaccg gctgctgccg aagagactcc tccgtacagt
     1321 aattacaaca cgcttccctg taggaagtcg cactgtcttt ccgagggtgc caccaaccca
     1381 caaattagcc ttagcaagag catgcaaggc agaagagcaa aaaccaccca ggatgttaac
     1441 acaggcgagg gcagcgagtt tgcggacagt gggatcgaag gggccaccac cgacacggac
     1501 ctcttgtcca ggagatccaa tgccaccaac tcaagctact cgccccccac aggccgagcc
     1561 tttgtgggca gcgacagcgg cagcagttca acaggggatg ctgcgcgcca gggggtgtac
     1621 gagaacttca ggcgggaact ggagatgagt accaccaaca gtgagagcct ggaggaggct
     1681 ggctctgcgc acagcgatga gcagagcagc ggaaccctca gttccccggg ccagtcggac
     1741 atcctgctaa cggctgcgca gggcactgtg cggaaggctg gagcgctggc agtcaagaac
     1801 ttcctggtcc acaagaagaa taagaaggtg gagtcggcta cccggaggaa gtggaagcac
     1861 tactgggtgt ccctgaaagg ttgcacgctc tttttctatg agactgatgg caggtctggg
     1921 atagaccaca acagtgtccc caagcacgcc gtctgggtgg agaacagcat cgtgcaggct
     1981 gtgcctgagc accccaagaa agacttcgtc ttctgcctca gcaactccct gggtgacgcc
     2041 ttcctcttcc agaccacgag ccagacagag ctggagaact ggatcaccgc catccactct
     2101 gcctgcgcag ctgccgtcgc aaggcaccac cacaaggagg acacgctccg cctcctcaag
     2161 tcggaaatca agaagctgga gcagaagatc gacatggacg agaagatgaa gaagatgggg
     2221 gagatgcagc tgtcttccgt caccgactcg aagaagaaga agaccatcct agaccagatc
     2281 ttcgtttggg agcagaacct cgaacagttc cagatggacc tgttccgatt ccgctgttac
     2341 ctcgccagcc tccagggcgg ggagctgcca aaccccaaaa ggctgcttgc ttttgcgagc
     2401 cggcccacga aggtggccat gggccgcctc gggatcttct ccgtgtcatc ttttcatgcc
     2461 ctggtggcag cccggactgg tgagatcgga gtgagaaggc gtactcaggc catgtccagg
     2521 tctgcaagca agcgaaggag caggttttct tctctctggg gcctagacac tacctccaaa
     2581 aagaagcagg gacgcccaac catcaaccag gtgtttggag aggggactga tgctgtaaag
     2641 agatctttag agggaatatt tgatgacact gttccagatg gcaagaggga gaaggaagtg
     2701 gtcttaccca gtgtccacca gcacaacccc gactgtgaca tttgggtcca tgaatatttc
     2761 actccatcct ggttctgtct acccaacaac cagccagcct tgacggttgt ccggccaggg
     2821 gacactgcga gggacacctt ggagctcatt tgcaagacac atcaactgga tcattccgcc
     2881 cattacctgc gcctgaaatt cctaatggag aacagagtgc agttctacat cccgcagccc
     2941 gaggaggaca tttacgagct gctttacaaa gaaattgaaa tctgtccaaa agtcacccag
     3001 aatatccaca ttgagaagtc agacgcggcc gctgataatt acgggttttt gctttcttct
     3061 gtggatgaag atggcattcg aaggctctac gtgaacagtg tcaaggaaac cgggttagct
     3121 tccaagaaag gcctgaaggc gggggatgag attctcgaga tcaataatcg tgctgctggt
     3181 accctgaact catctatgct caaagatttc ctctcacagc cctccctggg cctcctggtg
     3241 aggacctacc ctgagccaga gggaggcgtg gagctgctgg agaaccctcc gcaccgagtg
     3301 gacggccctg tggaccttgg cgagagcccc ctcgccttcc tcaccagcaa cccagggcac
     3361 agcctctcca gtgagcaggg cagcagtgct gagacagctc cagaagaggg cgaaggaccg
     3421 gacttggagt cttctgatga gacagatcac agcagcaaga gcacagaaca ggttgcagcg
     3481 ttttgccgga gtctgcacga gatgagcccc tctgactcaa gcccgtcccc tcaggatgcc
     3541 acaagccctc agctggcaac cacacgacag ctgtcggatg cggataagct gcgcaaggtg
     3601 atctgtgaac tgctggagac tgaacgcacc tatgtgaaag acttaaactg cctcatggag
     3661 agatacctga agccccttca gaaggagacc tttctcaccc aggatgagct tgatgtactt
     3721 tttggaaatt taaccgaaat ggtggagttt caagtcgagt tccttaagac tctagaagat
     3781 ggagtaagac tggtccctga cttggaaaag ctggagaagg ttgaccagtt caagaaagtg
     3841 ctcttctctc tggggggatc cttcctgtac tacgcggacc gcttcaagct ctacagtgcc
     3901 ttctgtgcga gccacacaaa agtccccaag gtcctggtga aagccaagac ggacacagcc
     3961 ttcaaggcgt tcctcgatgc ccagaacccg aggcagcagc actcatccac gctggagtct
     4021 tacctcatca agcccatcca gagggtcctc aagtacccgc ttctgctcag ggagctgttt
     4081 gcgctgaccg atgcggagag cgaggaacac taccacctgg acgtggccat caagactatg
     4141 aacaaggtcg ccagtcacat caatgagatg cagaagatcc acgaagagtt tggtgctgtg
     4201 tttgaccagc tgattgctga gcagacggga gagaagaaag aggttgcaga tctgagcatg
     4261 ggtgacctgc tcttgcacac cagcgtcatc tggctgaacc cacccgcctc actgggaaag
     4321 tggaaaaagg agccagaatt agcagccttc gtcttcaaaa cggccgtggt ccttgtatat
     4381 aaagacggtt ccaagcagaa gaagaaactt gttggctctc acaggctgtc aatctacgag
     4441 gagtgggacc ctttccggtt tcgccacatg atccctaccg aagctttgca ggtccgagct
     4501 ctgcccagtg cagatgcaga ggcaaatgcg gtatgcgaaa ttgtccacgt gaaatcagag
     4561 tcagaaggga ggcccgagcg ggttttccac ctctgctgca gttccccaga gagcagaaag
     4621 gactttctga agtctgtgca ttcgatcctg cgagataaac acagaagaca gctcctcaaa
     4681 acggaaagcc ttccgtcagc ccagcagtat gtcccctttg gaggcaagag attgtgtgcg
     4741 cttaaaggcg ccaggccggc catgagcagg gcagtgtctg ccccaagcaa gtctcttggg
     4801 aggaggaggc ggcgactggc ccgaaacagg tttaccatcg attcagacgc catctcagcc
     4861 agcagcccgg agaaagagcc ccagcagccc gccggtggtg gggacactga ccgatgggta
     4921 gaggaacagt tcgatcttgc tcagtacgag gagcaggatg acatcaagga gacagacatc
     4981 ctcagtgacg atgacgaatt ctgtgagtcc ctgaagggcg cctcagtgga cagagacctt
     5041 caggagcagc ttcaggctgc ctccatcagt cagcgggccc gaggccggag aaccctcgat
     5101 agccacgcct cccgcatgac acagctcaag aagcaagcgg ccctctcggg catcaacggg
     5161 ggcctggaga gtgcgagcga ggaagtcatt tgggtcaggc gcgaagactt tgccccctcc
     5221 aggaaactga acacggagat atgacttcct tgtccctgta gagaatatgt gtgtagatat
     5281 gatgccggcc ccaccctcac tccgcccacc ctcctgtgcc gtccacgaga atgtcccctt
     5341 aggtcgcact cttgacacag attttggcag ctgacttggg tctgaagcca tgtagccacc
     5401 caccttcatc attcttaatg acaccagaga gaatcgttca gaatcccaga caaacaagct
     5461 tgagtccttt cctctgtgcc aagggctttc ttttatttat tctcaattca tcggggcctg
     5521 aagatttaga acgaagaacc gatagcgcta gaaatcaaac cgtccccacc ccagcagtca
     5581 aaacagggac aggacggtgt gcacagattg tcccagctcc acctctctaa atccagtctc
     5641 gcagctgagg aacaaccacc ttcaaacaca catagagaaa cggaacagat ggaaacacct
     5701 gtacagcgtg cagtgttctg actcctcccc tcttcagcgt ccgagaatat ctgtgtatct
     5761 taagaatgtg tgaggaaagg gtggtcgtct ctgtacgatg agcctttatt cttatttttt
     5821 ttttcttctg ttcatgtcta tagctggtct tactctgtgt cagtgtctgg aagctctagt
     5881 tttgcagaga attataaaga tgccaaactc ttggaaaaga gatccaaatt tatcacttag
     5941 agggagagag aagaaaaaaa agaaacacta ttttttgtat tttccttgag atgcaggggc
     6001 acaaatagat gagaatttta cagtgttagg tgtaactcag tctgaaaatg aggatataac
     6061 cttttgcaga gcaagcgggg cacgatgact ttatatttat acacaaacgg ccaacaagaa
     6121 gctcatgcta agaataaact tttctgagaa acagattttt tttttaaatg tatgaatcta
     6181 tatttgagat gggagtttgg ttggattaca gggacaggaa atggggcagt gtgtgcgtgt
     6241 gtctgttgtt catggtgggg gaggggagcc tcctcatggg gttgccatgg caatcattgg
     6301 ttttcccatc aaacttgcat cttcatccac agatgacctc cccttccctg agagtccacg
     6361 gcagaatctt agaacctctt cagagacgtc ctttgtggtt aatatttcct ccatgccaat
     6421 gagacagggt aaactcgcta cggggcctaa ccttctaaat ggatgtccag acaggaagaa
     6481 caaaacccat tctagcactt tcatttttaa aaaaaaaaag gtttatagct ttttgttccg
     6541 ccctcctcct ccccatttca caatgtccat tccttaagaa gggtttaaat aatatgaaaa
     6601 ctttgttttt ggaaataaaa aaatatctat atatctatat atctatatat ctatttggtg
     6661 tttgatacat cggtaggtac tttaaagacc tgaattttat agtagcttta gcaattatat
     6721 tttataagaa tcagttatga ctttatattt ccagatagag agttcagaac accatgctct
     6781 aaagcctccc ccaccccggc ttgctttcct gctttcctga cctctctccc ccccccccct
     6841 ttcgggtttc cagggctttg agcttgatct tttgaaagtt ttattctatt acagtttttg
     6901 ctattacctt ctggttttct taagaagctt tagagtggtt tctatgccct ttgtaccact
     6961 gcactgttcg taacatctcg gggtcgattg cgtccggatg ggaaactgaa gcagaggtgt
     7021 cggatagtca cattttctgg tctccctgca acacgtccat cggaaaaaaa aatactggaa
     7081 agtctggtgc ttagagaggg tgccattgtc tcttgtatat acggtcatga tgtgtctatg
     7141 tcaaaagttc ttatatattt cttttataag ctgaaagaag gtctattttt atgtttttag
     7201 gtctatgaat ggaacgttgt aaatgcctgt ccaacaataa aagtactgaa aagggaaaaa
     7261 aaaaaaaaaa aaaaaaa
//
