LOCUS       NM_001409955            2706 bp    mRNA    linear   ROD 15-DEC-2024
DEFINITION  Mus musculus THUMP domain containing 3 (Thumpd3), transcript
            variant 4, mRNA.
ACCESSION   NM_001409955 XM_006505579
VERSION     NM_001409955.1
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2706)
  AUTHORS   McKee,A.E., Minet,E., Stern,C., Riahi,S., Stiles,C.D. and
            Silver,P.A.
  TITLE     A genome-wide in situ hybridization map of RNA-binding proteins
            reveals anatomically restricted expression in the developing mouse
            brain
  JOURNAL   BMC Dev Biol 5, 14 (2005)
   PUBMED   16033648
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2706)
  AUTHORS   Zambrowicz,B.P., Imamoto,A., Fiering,S., Herzenberg,L.A., Kerr,W.G.
            and Soriano,P.
  TITLE     Disruption of overlapping transcripts in the ROSA beta geo 26 gene
            trap strain leads to widespread expression of beta-galactosidase in
            mouse embryos and hematopoietic cells
  JOURNAL   Proc Natl Acad Sci U S A 94 (8), 3789-3794 (1997)
   PUBMED   9108056
REFERENCE   3  (bases 1 to 2706)
  AUTHORS   Friedrich,G. and Soriano,P.
  TITLE     Promoter traps in embryonic stem cells: a genetic screen to
            identify and mutate developmental genes in mice
  JOURNAL   Genes Dev 5 (9), 1513-1523 (1991)
   PUBMED   1653172
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC155722.9.
            
            On Jul 25, 2022 this sequence version replaced XM_006505579.3.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR17253012.6312450.1,
                                           SRR13948564.4615466.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849381, SAMN00849382
                                           [ECO:0000348]
            ##Evidence-Data-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-709               AC155722.9         150481-151189
            710-999             AC155722.9         151413-151702
            1000-1077           AC155722.9         153337-153414
            1078-1554           AC155722.9         159637-160113
            1555-1685           AC155722.9         163953-164083
            1686-1755           AC155722.9         167099-167168
            1756-1871           AC155722.9         169542-169657
            1872-1982           AC155722.9         170754-170864
            1983-2106           AC155722.9         171266-171389
            2107-2706           AC155722.9         171672-172271
FEATURES             Location/Qualifiers
     source          1..2706
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="6"
                     /map="6 52.73 cM"
     gene            1..2706
                     /gene="Thumpd3"
                     /gene_synonym="Gt(ROSA)26asSor; Gtrosa26as"
                     /note="THUMP domain containing 3"
                     /db_xref="GeneID:14911"
                     /db_xref="MGI:MGI:1277973"
     exon            1..709
                     /gene="Thumpd3"
                     /gene_synonym="Gt(ROSA)26asSor; Gtrosa26as"
                     /inference="alignment:Splign:2.1.0"
     exon            710..999
                     /gene="Thumpd3"
                     /gene_synonym="Gt(ROSA)26asSor; Gtrosa26as"
                     /inference="alignment:Splign:2.1.0"
     CDS             742..2259
                     /gene="Thumpd3"
                     /gene_synonym="Gt(ROSA)26asSor; Gtrosa26as"
                     /EC_number="2.1.1.256"
                     /note="gene trap ROSA 26 antisense, Philippe Soriano;
                     THUMP domain-containing protein 3; gtROSA26asSor; tRNA
                     (guanine(6)-N2)-methyltransferase THUMP3"
                     /codon_start=1
                     /product="tRNA (guanine(6)-N(2))-methyltransferase THUMP3"
                     /protein_id="NP_001396884.1"
                     /db_xref="GeneID:14911"
                     /db_xref="MGI:MGI:1277973"
                     /translation="MSCDTQEATRECLGMNLDGNKEPVSLVESGVRSESEHLQVTIGA
                     TVPTGFEQTAAGEVREKLKSACRISKDRGKIYFDIAVESLAQVHCLRSVDNLFVVVQE
                     FKDYQFKDTKEEVLRDFEELAGKLPWSDPLKVWQINTTFKKKKAKRRKANQSAGKEKA
                     DCGQGDKADEKDGKKKHASSTSDSHILDYYENPAIKEEISTLVGDVLSSCKDETGQSL
                     REETEPQVQKFRVTCNRAGEKHCFTSNEAARDFGGAIQEYFKWKADMTNFDVEVLLNI
                     HDNEVIVAIALTEESLHRRNITHFGPTTLRSTLAYGMLRLCEPKPTDVIVDPMCGTGA
                     IPIEGATEWSHCYHIAGDNNPLAVNRAANNISSLLTKSQIKDGKTTWGLPIDAVQWDI
                     CNLPLRTASVDIIVTDMPFGKRMGSKKRNWNLYPACLREMSRVCRPGTGRAVLLTQDK
                     KCFTKALSGMGHVWRKVHVVWVNIGGLHAAVYLLKRTAQAFVHPSDQDEGRDPPW"
     exon            1000..1077
                     /gene="Thumpd3"
                     /gene_synonym="Gt(ROSA)26asSor; Gtrosa26as"
                     /inference="alignment:Splign:2.1.0"
     exon            1078..1554
                     /gene="Thumpd3"
                     /gene_synonym="Gt(ROSA)26asSor; Gtrosa26as"
                     /inference="alignment:Splign:2.1.0"
     exon            1555..1685
                     /gene="Thumpd3"
                     /gene_synonym="Gt(ROSA)26asSor; Gtrosa26as"
                     /inference="alignment:Splign:2.1.0"
     exon            1686..1755
                     /gene="Thumpd3"
                     /gene_synonym="Gt(ROSA)26asSor; Gtrosa26as"
                     /inference="alignment:Splign:2.1.0"
     exon            1756..1871
                     /gene="Thumpd3"
                     /gene_synonym="Gt(ROSA)26asSor; Gtrosa26as"
                     /inference="alignment:Splign:2.1.0"
     exon            1872..1982
                     /gene="Thumpd3"
                     /gene_synonym="Gt(ROSA)26asSor; Gtrosa26as"
                     /inference="alignment:Splign:2.1.0"
     exon            1983..2106
                     /gene="Thumpd3"
                     /gene_synonym="Gt(ROSA)26asSor; Gtrosa26as"
                     /inference="alignment:Splign:2.1.0"
     exon            2107..2706
                     /gene="Thumpd3"
                     /gene_synonym="Gt(ROSA)26asSor; Gtrosa26as"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2586..2591
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Thumpd3"
                     /gene_synonym="Gt(ROSA)26asSor; Gtrosa26as"
                     /note="hexamer: AATAAA"
     polyA_site      2608
                     /gene="Thumpd3"
                     /gene_synonym="Gt(ROSA)26asSor; Gtrosa26as"
                     /note="major polyA site"
     regulatory      2686..2691
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Thumpd3"
                     /gene_synonym="Gt(ROSA)26asSor; Gtrosa26as"
                     /note="hexamer: ATTAAA"
     polyA_site      2706
                     /gene="Thumpd3"
                     /gene_synonym="Gt(ROSA)26asSor; Gtrosa26as"
                     /note="major polyA site"
ORIGIN      
        1 ggactctcgc tgggcttcgc agagggtggg agtgagagag ccactgccct tcggtccgaa
       61 cagcccttct gctctccaca ctgtccgtag gagttgtgtt cacagtggcg aacggtgaca
      121 cggtggaacc gcgttcacct ctgtggcctg ttcttggttg gcacagcatt agggacaagg
      181 aagatgttag cttaagccac ggcgcttttg aaaagctggt gggaggtgtg ggccctccat
      241 cacggtaaca ccactccagc cccttcaacc tccacaaaca ctcccacagg acgtctgtat
      301 tactagcttc agacatttcc aaggtgtccc cagcatgcgc gtcattggat ctcggcgtca
      361 ccacatagta ggctttcaga tatttgtaat tgtttagata gtggaagttg agttaatctt
      421 taataaattc actgcccact tgtctacatg gcggaaagta gatacctcac ccatacatta
      481 aaagttttga tcattagtcc agaattgtgt tgttctcgtt aaaatcttct gtctttggga
      541 aagagagata tatttagttt ccagttcgaa actaggcagg agtaacttgc ttcctggacc
      601 cgcgagtgtg ggaagcaaga atttttaact ctgaaccact acagcctcac atcctgaagt
      661 gctaagctga aagagcaaca gaggagcttc tctattcatg ttagcaaata gactgttggt
      721 ccttttagaa acatctccat catgtcttgt gacactcaag aagctaccag agagtgcctg
      781 ggtatgaacc ttgatggcaa caaagagcct gtgtcgctgg tagaaagcgg cgtcagaagt
      841 gagtcggagc atctccaagt cactattgga gccactgtac ccactggctt tgaacaaacg
      901 gctgcggggg aagtgagaga gaaactgaag tcggcctgca gaatcagcaa agaccgcgga
      961 aagatctatt ttgatattgc agtggaaagt ctggctcagg ttcattgtct gagatcagtt
     1021 gataacttgt ttgtggttgt tcaggagttt aaagattacc agttcaaaga tacgaaggaa
     1081 gaagttctaa gagactttga agaactggct ggaaaactcc catggtcaga ccctttaaaa
     1141 gtctggcaaa ttaacaccac tttcaagaag aagaaagcaa agcgcagaaa ggcaaatcag
     1201 agtgcaggta aagagaaggc tgactgtgga caaggagaca aagcagatga gaaagatggt
     1261 aagaaaaagc atgccagcag cacttcagat tcacatatct tggactatta tgaaaatcca
     1321 gccatcaaag aagagatatc aaccttagta ggtgatgtct tgtcgtcttg caaagatgaa
     1381 actggtcaaa gcttaagaga agaaactgaa ccacaggtac agaagtttag agtcacctgc
     1441 aacagagcag gagagaaaca ttgctttacc tccaatgagg ctgcgagaga ttttgggggt
     1501 gctattcaag agtactttaa gtggaaggct gatatgacca actttgatgt agaggttctc
     1561 ctgaacatcc atgataatga agtcattgtt gctattgcac tgacagaaga gagtctccat
     1621 cgcagaaata ttacacattt tggacctaca actcttaggt caactcttgc ctatgggatg
     1681 ctcaggctct gtgaacctaa gcctactgat gtaatagtgg acccaatgtg tggaacaggg
     1741 gcaataccaa tagagggggc tactgagtgg tctcactgtt accatattgc tggggacaat
     1801 aacccactgg cagtgaacag agcagcaaat aacatctcat ctctattgac taagagccag
     1861 attaaagatg gaaaaacaac ctggggtttg cccattgatg ctgttcagtg ggatatctgc
     1921 aacctcccac tgagaactgc ttctgtggat attattgtaa cagatatgcc atttggaaaa
     1981 aggatgggat ccaagaagag aaattggaat ctctatccag cttgccttcg ggaaatgagc
     2041 cgtgtctgta gaccagggac aggcagagct gtactgctta ctcaggacaa gaaatgtttt
     2101 accaaggcct tatctggaat gggacatgtg tggcgaaagg tccatgtagt ctgggtgaac
     2161 atcgggggcc ttcatgctgc agtttatctt ctaaagcgca ctgctcaagc ctttgttcat
     2221 ccttcagatc aagatgaagg aagagaccct ccttggtaaa gaaaagagtg aagacaactt
     2281 attaatattt gtagttccta acactggaaa tatcagcata aagaacttgc tttgggagaa
     2341 aaatagcaga aaagtaactt acagtacagg ttacactgct tgaccactcc agaatgcttg
     2401 atttctagca aggtgattgt aatggtattt cttaagaagc ctacactgct tggcttctaa
     2461 gtgtcagaac actttaggcc atattctatt gcttgtgcaa cctactgttt tatggtctaa
     2521 attctttgta tcatctcaga agcagaagca tcccttaaga tctacagttt tatcatctgc
     2581 cttaaaataa atatacaacc taaacagagc aaagatgctt gtgtgactat tataatatta
     2641 ctaagtagta gaaacaccag tagatgtatg gaaccaacaa gcctaattaa agaaatttct
     2701 ctttta
//
