LOCUS       NM_153083               2839 bp    mRNA    linear   ROD 04-JUN-2024
DEFINITION  Mus musculus thiamine triphosphatase (Thtpa), mRNA.
ACCESSION   NM_153083 XM_487337
VERSION     NM_153083.5
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2839)
  AUTHORS   Bedogni,F. and Hevner,R.F.
  TITLE     Cell-Type-Specific Gene Expression in Developing Mouse Neocortex:
            Intermediate Progenitors Implicated in Axon Development
  JOURNAL   Front Mol Neurosci 14, 686034 (2021)
   PUBMED   34321999
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2839)
  AUTHORS   Delvaux,D., Kerff,F., Murty,M.R., Lakaye,B., Czerniecki,J.,
            Kohn,G., Wins,P., Herman,R., Gabelica,V., Heuze,F., Tordoir,X.,
            Maree,R., Matagne,A., Charlier,P., De Pauw,E. and Bettendorff,L.
  TITLE     Structural determinants of specificity and catalytic mechanism in
            mammalian 25-kDa thiamine triphosphatase
  JOURNAL   Biochim Biophys Acta 1830 (10), 4513-4523 (2013)
   PUBMED   23707715
REFERENCE   3  (bases 1 to 2839)
  AUTHORS   Song,J., Bettendorff,L., Tonelli,M. and Markley,J.L.
  TITLE     Structural basis for the catalytic mechanism of mammalian 25-kDa
            thiamine triphosphatase
  JOURNAL   J Biol Chem 283 (16), 10939-10948 (2008)
   PUBMED   18276586
  REMARK    GeneRIF: analysis of the catalytic mechanism of mouse 25-kDa
            thiamine triphosphatase
REFERENCE   4  (bases 1 to 2839)
  AUTHORS   Suryo Rahmanto,Y., Dunn,L.L. and Richardson,D.R.
  TITLE     Identification of distinct changes in gene expression after
            modulation of melanoma tumor antigen p97 (melanotransferrin) in
            multiple models in vitro and in vivo
  JOURNAL   Carcinogenesis 28 (10), 2172-2183 (2007)
   PUBMED   17449903
REFERENCE   5  (bases 1 to 2839)
  AUTHORS   Lakaye,B., Verlaet,M., Dubail,J., Czerniecki,J., Bontems,S.,
            Makarchikov,A.F., Wins,P., Piette,J., Grisar,T. and Bettendorff,L.
  TITLE     Expression of 25 kDa thiamine triphosphatase in rodent tissues
            using quantitative PCR and characterization of its mRNA
  JOURNAL   Int J Biochem Cell Biol 36 (10), 2032-2041 (2004)
   PUBMED   15203116
  REMARK    GeneRIF: The highest amount of mRNA was found in testis, followed
            by lung and muscle, while the highest enzyme activities were found
            in liver and kidney
REFERENCE   6  (bases 1 to 2839)
  AUTHORS   Czerniecki,J., Chanas,G., Verlaet,M., Bettendorff,L.,
            Makarchikov,A.F., Leprince,P., Wins,P., Grisar,T. and Lakaye,B.
  TITLE     Neuronal localization of the 25-kDa specific thiamine
            triphosphatase in rodent brain
  JOURNAL   Neuroscience 125 (4), 833-840 (2004)
   PUBMED   15120844
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC157212.2.
            
            On Mar 17, 2010 this sequence version replaced NM_153083.4.
            
            Summary: This gene encodes a cytosolic enzyme that catalyzes the
            conversion of thiamine triphosphate to thiamine diphosphate. The
            encoded protein requires divalent cations for enzymatic activity.
            The gene product is believed to play a role in regulation of
            thiamine triphosphate intracellular concentration, and it may be a
            messenger in cell signaling in response to cellular stress.
            [provided by RefSeq, Mar 2010].
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF432864.2, AK132479.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1223              AC157212.2         143249-144471
            1224-2839           AC157212.2         145845-147460
FEATURES             Location/Qualifiers
     source          1..2839
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="14"
                     /map="14 28.07 cM"
     gene            1..2839
                     /gene="Thtpa"
                     /note="thiamine triphosphatase"
                     /db_xref="GeneID:105663"
                     /db_xref="MGI:MGI:2446078"
     exon            1..1223
                     /gene="Thtpa"
                     /inference="alignment:Splign:2.1.0"
     CDS             677..1351
                     /gene="Thtpa"
                     /EC_number="3.6.1.28"
                     /note="ThTPase"
                     /codon_start=1
                     /product="thiamine-triphosphatase"
                     /protein_id="NP_694723.1"
                     /db_xref="CCDS:CCDS27108.1"
                     /db_xref="GeneID:105663"
                     /db_xref="MGI:MGI:2446078"
                     /translation="MAQGLIEVERKFAPGPDTEERLQELGATLEHRVTFRDTYYDTSE
                     LSLMLSDHWLRQREGSGWELKCPGVTGVSGPHNEYVEVTSEAAIVAQLFELLGSGEQK
                     PAGVAAVLGSLKLQEVASFITTRSSWKLALSGAHGQEPQLTIDLDSADFGYAVGEVEA
                     MVHEKAEVPAALEKIITVSSMLGVPAQEEAPAKLMVYLQRFRPLDYQRLLEAASSGEA
                     TGDSAS"
     exon            1224..2839
                     /gene="Thtpa"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2808..2813
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Thtpa"
                     /note="hexamer: AATAAA"
     polyA_site      2826
                     /gene="Thtpa"
                     /note="major polyA site"
ORIGIN      
        1 gatgccaggc tcacgctaag gctcgagatc ttggccgatt cctgggctcc aggaaaaatg
       61 caggatgaac agcgggcaag aggaagctct ctcttctctt gcccaccggg ttgggtcgca
      121 cgtaacctaa cagacctctc aggcctagct tgtcatcggc accgccacac cggtctaata
      181 tggccgctag tgctgggacc ggtctcgcgc gtaaagagcc tggactaagg tccggagccg
      241 gagaccgctc ccggcattcc tcgcgggtgt agcgtgggca cccttccccc acttccgggt
      301 cccgggagaa ggagaccctg gggtcttgaa gagtgtcccg aggtaccgct gtttggtgct
      361 aggcgaagaa tggcagatca tcctgggcag tgaatcactg gccttgggta gcaaggtgta
      421 gcgagaagat aggtcatcag cattacggcc tgtttgctag ggagcctttt gggttatcag
      481 tagtaggatt taactggaga cttgtcacca ttgttgcagc ccgcagagac ctccaagtag
      541 agccctgtga acctggagca gagctctggg acagtgtata tgcatcttaa attgatccag
      601 gactagtccg tctttgctga ctagtcctca ccttgagcac tcagtgaata cctggcaaac
      661 agggttgcag gttaatatgg cccagggctt gattgaagtg gagcgaaagt tcgctccagg
      721 gcctgacacg gaggaaaggc tgcaggagtt gggggccacc ctggagcacc gggtcacctt
      781 ccgagacaca tactatgata cctctgagtt aagccttatg ctgtctgacc actggctgcg
      841 acaacgagaa ggtagtggat gggagctcaa atgtcctgga gtaacaggtg tctcaggacc
      901 tcacaatgag tatgtggaag tcacatcgga agctgcgatt gtggcccagc tctttgaact
      961 gctcgggtct ggggagcaga agcctgcagg tgtggctgct gtcctgggct cgctgaagct
     1021 gcaggaggtg gctagtttta ttactacgcg aagctcctgg aagctggcct tatctggagc
     1081 ccatggacag gagcctcagc tcacaataga cttggattca gcggacttcg gctatgctgt
     1141 gggtgaggta gaggccatgg tgcacgagaa ggctgaagtc ccggctgccc tagagaagat
     1201 catcaccgtc agtagcatgc ttggagtgcc agcacaggag gaggcaccag ccaagctcat
     1261 ggtataccta cagcgcttca ggcctctgga ctatcagcgc ctgctagaag cagccagttc
     1321 tggtgaggcc acaggggact cagcatcctg acaacagcgt tcactttcca aaagggaagg
     1381 taactctgaa gccgccattc acatccggac ccacggtgct cctttccctt acaacggagc
     1441 cactttcccc tccagctctg cctggtcttt cgcccactct gagctatcct tcctggagcc
     1501 ctccctggct gcttccctca tccgtcagat gcaatctgtg ggccgcccct ctcccctggc
     1561 cgctaagcag cctccattga tttccgctcg tgtttatagg atttccactt agccgtgatc
     1621 agtagttaag cacaggaaaa tcccttgcca cccccccctc ccttggtcga tgccattgat
     1681 tctgccagag gctctgaagc cgccttacag ctgagttaga gatgagggga aacagcgggg
     1741 atttccctgc cctggggtgt agggaagagc aagaaggtgg ggaaaaggtt aggaactcct
     1801 taagggatct agaaattcag ggcctgctgc tgtgccggga gggtagagtg ctcagtgtgg
     1861 cagtttagag agaacatttg ctctccaacc cagagagggg tagaaagaaa atggtcattg
     1921 ttgagaccgg ttcctagggt ttcaggaagt catacttcct gtggagaagg caccaccctt
     1981 gctcttaaca catttttttt tctctcaact aggtcaaaac aggcctctct tcccttctca
     2041 cgcttcatag actttatttg ttggggagga ggcaggctct ctatattgcc ttgacaaata
     2101 tagggtctga gtgccacctg ctggtggccc tcagggttgt gtcccaagct gagggtgcag
     2161 aaagatttga gaggagccga ggcagttatg aggcaggcaa gcttagggaa caggttagca
     2221 gcctggaact ccacttggtt tgagctatgc acacctctca tctcactgcc acttggcatc
     2281 ttaggaccct cagctgcacg caccatgcac acacattctt ctaacagtgc agccaggacc
     2341 ccgcctgtat cgagtagcac ttagagccca cttcagacag taaatctctc tttccccagc
     2401 agccatcaaa actccccttc tgtggacagt tttattcaaa cactttagga cataccccaa
     2461 agtagggagc tgattaggcc taaacgggtt tggggtaacc tgtgatcttt gggtgacctt
     2521 ttgaggagaa ctccctatgg accgtcctat gcctgtgcag aatgagttag cttatgatta
     2581 aagtcagctg catcatggga agggttacgc acagagaaga aaggctgaca gaatccaagc
     2641 ctgtcaatga aaacagagaa tcactcaaat tatgccactt cacagctaac accttcgttc
     2701 cctgagactt tattaaagga acaccgggtg actgccctgg gctcttaaga ctgtggtcat
     2761 cacccgttta cagagcactt ctaatttgta cgctcagttt gcctttaaat aaagtttact
     2821 tcgccattta cgtgggtct
//
