LOCUS       NM_019576               4432 bp    mRNA    linear   ROD 09-FEB-2025
DEFINITION  Mus musculus thrombospondin, type I, domain 1 (Thsd1), transcript
            variant 1, mRNA.
ACCESSION   NM_019576 XM_981252
VERSION     NM_019576.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4432)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 4432)
  AUTHORS   Dickinson,M.E., Flenniken,A.M., Ji,X., Teboul,L., Wong,M.D.,
            White,J.K., Meehan,T.F., Weninger,W.J., Westerberg,H., Adissu,H.,
            Baker,C.N., Bower,L., Brown,J.M., Caddle,L.B., Chiani,F., Clary,D.,
            Cleak,J., Daly,M.J., Denegre,J.M., Doe,B., Dolan,M.E., Edie,S.M.,
            Fuchs,H., Gailus-Durner,V., Galli,A., Gambadoro,A., Gallegos,J.,
            Guo,S., Horner,N.R., Hsu,C.W., Johnson,S.J., Kalaga,S., Keith,L.C.,
            Lanoue,L., Lawson,T.N., Lek,M., Mark,M., Marschall,S., Mason,J.,
            McElwee,M.L., Newbigging,S., Nutter,L.M., Peterson,K.A.,
            Ramirez-Solis,R., Rowland,D.J., Ryder,E., Samocha,K.E.,
            Seavitt,J.R., Selloum,M., Szoke-Kovacs,Z., Tamura,M., Trainor,A.G.,
            Tudose,I., Wakana,S., Warren,J., Wendling,O., West,D.B., Wong,L.,
            Yoshiki,A., MacArthur,D.G., Tocchini-Valentini,G.P., Gao,X.,
            Flicek,P., Bradley,A., Skarnes,W.C., Justice,M.J., Parkinson,H.E.,
            Moore,M., Wells,S., Braun,R.E., Svenson,K.L., de Angelis,M.H.,
            Herault,Y., Mohun,T., Mallon,A.M., Henkelman,R.M., Brown,S.D.,
            Adams,D.J., Lloyd,K.C., McKerlie,C., Beaudet,A.L., Bucan,M. and
            Murray,S.A.
  CONSRTM   International Mouse Phenotyping Consortium; Jackson Laboratory;
            Infrastructure Nationale PHENOMIN, Institut Clinique de la Souris
            (ICS); Charles River Laboratories; MRC Harwell; Toronto Centre for
            Phenogenomics; Wellcome Trust Sanger Institute; RIKEN BioResource
            Center
  TITLE     High-throughput discovery of novel developmental phenotypes
  JOURNAL   Nature 537 (7621), 508-514 (2016)
   PUBMED   27626380
  REMARK    Erratum:[Nature. 2017 Nov 16;551(7680):398. doi:
            10.1038/nature24643.. PMID: 29144450]
REFERENCE   3  (bases 1 to 4432)
  AUTHORS   Haasdijk,R.A., Den Dekker,W.K., Cheng,C., Tempel,D., Szulcek,R.,
            Bos,F.L., Hermkens,D.M., Chrifi,I., Brandt,M.M., Van Dijk,C.,
            Xu,Y.J., Van De Kamp,E.H., Blonden,L.A., Van Bezu,J., Sluimer,J.C.,
            Biessen,E.A., Van Nieuw Amerongen,G.P. and Duckers,H.J.
  TITLE     THSD1 preserves vascular integrity and protects against intraplaque
            haemorrhaging in ApoE-/- mice
  JOURNAL   Cardiovasc Res 110 (1), 129-139 (2016)
   PUBMED   26822228
  REMARK    GeneRIF: THSD1 is a new regulator of endothelial barrier function
            during vascular development and protects intraplaque microvessels
            against haemorrhaging in advanced atherosclerotic lesions
REFERENCE   4  (bases 1 to 4432)
  AUTHORS   Koscielny,G., Yaikhom,G., Iyer,V., Meehan,T.F., Morgan,H.,
            Atienza-Herrero,J., Blake,A., Chen,C.K., Easty,R., Di Fenza,A.,
            Fiegel,T., Grifiths,M., Horne,A., Karp,N.A., Kurbatova,N.,
            Mason,J.C., Matthews,P., Oakley,D.J., Qazi,A., Regnart,J.,
            Retha,A., Santos,L.A., Sneddon,D.J., Warren,J., Westerberg,H.,
            Wilson,R.J., Melvin,D.G., Smedley,D., Brown,S.D., Flicek,P.,
            Skarnes,W.C., Mallon,A.M. and Parkinson,H.
  TITLE     The International Mouse Phenotyping Consortium Web Portal, a
            unified point of access for knockout mice and related phenotyping
            data
  JOURNAL   Nucleic Acids Res 42 (Database issue), D802-D809 (2014)
   PUBMED   24194600
REFERENCE   5  (bases 1 to 4432)
  AUTHORS   Tang,T., Li,L., Tang,J., Li,Y., Lin,W.Y., Martin,F., Grant,D.,
            Solloway,M., Parker,L., Ye,W., Forrest,W., Ghilardi,N., Oravecz,T.,
            Platt,K.A., Rice,D.S., Hansen,G.M., Abuin,A., Eberhart,D.E.,
            Godowski,P., Holt,K.H., Peterson,A., Zambrowicz,B.P. and de
            Sauvage,F.J.
  TITLE     A mouse knockout library for secreted and transmembrane proteins
  JOURNAL   Nat Biotechnol 28 (7), 749-755 (2010)
   PUBMED   20562862
REFERENCE   6  (bases 1 to 4432)
  AUTHORS   Takayanagi,S., Hiroyama,T., Yamazaki,S., Nakajima,T., Morita,Y.,
            Usui,J., Eto,K., Motohashi,T., Shiomi,K., Keino-Masu,K., Masu,M.,
            Oike,Y., Mori,S., Yoshida,N., Iwama,A. and Nakauchi,H.
  TITLE     Genetic marking of hematopoietic stem and endothelial cells:
            identification of the Tmtsp gene encoding a novel cell surface
            protein with the thrombospondin-1 domain
  JOURNAL   Blood 107 (11), 4317-4325 (2006)
   PUBMED   16455951
  REMARK    GeneRIF: Expression profiling of Tmtsp demonstrated its specific
            expression in hematopoietic and endothelial cells
REFERENCE   7  (bases 1 to 4432)
  AUTHORS   Desai,J., Shannon,M.E., Johnson,M.D., Ruff,D.W., Hughes,L.A.,
            Kerley,M.K., Carpenter,D.A., Johnson,D.K., Rinchik,E.M. and
            Culiat,C.T.
  TITLE     Nell1-deficient mice have reduced expression of extracellular
            matrix proteins causing cranial and vertebral defects
  JOURNAL   Hum Mol Genet 15 (8), 1329-1341 (2006)
   PUBMED   16537572
REFERENCE   8  (bases 1 to 4432)
  AUTHORS   Bulfone,A., Carotenuto,P., Faedo,A., Aglio,V., Garzia,L.,
            Bello,A.M., Basile,A., Andre,A., Cocchia,M., Guardiola,O.,
            Ballabio,A., Rubenstein,J.L. and Zollo,M.
  TITLE     Telencephalic embryonic subtractive sequences: a unique collection
            of neurodevelopmental genes
  JOURNAL   J Neurosci 25 (33), 7586-7600 (2005)
   PUBMED   16107646
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC117665.6 and AC121933.3.
            
            On Apr 6, 2011 this sequence version replaced NM_019576.1.
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (1).
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK049636.1, SRR11927932.2460897.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849385, SAMN00849387
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-127               AC117665.6         194666-194792
            128-270             AC117665.6         205818-205960
            271-1230            AC117665.6         210360-211319
            1231-1389           AC121933.3         72825-72983         c
            1390-4432           AC121933.3         63802-66844         c
FEATURES             Location/Qualifiers
     source          1..4432
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="8"
                     /map="8 11.16 cM"
     gene            1..4432
                     /gene="Thsd1"
                     /gene_synonym="4833423O18Rik; Tmtsp"
                     /note="thrombospondin, type I, domain 1"
                     /db_xref="GeneID:56229"
                     /db_xref="MGI:MGI:1929096"
     exon            1..127
                     /gene="Thsd1"
                     /gene_synonym="4833423O18Rik; Tmtsp"
                     /inference="alignment:Splign:2.1.0"
     exon            128..270
                     /gene="Thsd1"
                     /gene_synonym="4833423O18Rik; Tmtsp"
                     /inference="alignment:Splign:2.1.0"
     CDS             213..2768
                     /gene="Thsd1"
                     /gene_synonym="4833423O18Rik; Tmtsp"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1; thrombospondin type-1 domain-containing protein
                     1; transmembrane molecule with thrombospondin module"
                     /codon_start=1
                     /product="thrombospondin type-1 domain-containing protein
                     1 isoform 1 precursor"
                     /protein_id="NP_062522.1"
                     /db_xref="CCDS:CCDS22172.1"
                     /db_xref="GeneID:56229"
                     /db_xref="MGI:MGI:1929096"
                     /translation="MKPMLKDFSNLLLVVLCDYVLGEAEYLLLQEPVHVALSDRTVSV
                     GFHYLSDVNGTLRNVSVMLWEANTNRTLTTKYLLTNQAQGTLQFECFYFKEAGDYWFV
                     MIPEVTDNGTQVPLWEKSAFLKVEWPVFHIDLNRTAKAAEGTFQVGVFTTQPLCLFPV
                     DKPDMLVDVIFTDRLPEARASLGQPLEIRASKRTKLTQGQWVEFGCAPVGVEAYVTVM
                     LRLLGQDSVIASTGPIDLAQKFGYKLMMAPEVTCESVLEVMVLPPPCVFVQGVLAVYK
                     EAPKRPEERTFQVAENRLPLGERRTVFNCTLFDVGKNKYCFNFGIVKKGHFSAKECML
                     IQRNIETWGPWQPWSPCSTTCGDAVRERRRLCVTSFPSRPSCSGMSSETSPCSLEECA
                     VFRPPGPSPVSPQDPVKSNNVVTVTGISLCLFIIFATVLITLWRRFGRAPKCSTPVRH
                     NSIHSPGFRKNSDEENICELSEPRGSFSDAGDGPRGSPGDTGIPLTYRCSASAPPEDE
                     ASGSESFQSNAQKIIPPLFSYRLAQQQLKEMKKKGLTETTKVYHVSQSPLTDTVVDAT
                     ASPPLDLECPEEAAASKFRIKSPFLDQPGAGTGERPPSRLDGIVPPPGCAVSPSQTLI
                     RKSQIRSTGGRDGSSERCHSRSSLFRRTASFHETKQSRPFRERSLSALTPRQVPAYSS
                     RMRTWDQMEDRCRPPSRSTHLLPERPEHFQGAGRTSSPLGPLSKSYTVGHPRRKPDPG
                     DRQAGLVAGAEKMEPHRAHRGPSPSHRSASRKQSSPIFLKDSYQKVSQLSPSHFRKDK
                     CQSFPIHPEFAFYDNTSFRLTEAEQRMLDLPGYFGSNEEDETTSTLSVEKLVI"
     exon            271..1230
                     /gene="Thsd1"
                     /gene_synonym="4833423O18Rik; Tmtsp"
                     /inference="alignment:Splign:2.1.0"
     exon            1231..1389
                     /gene="Thsd1"
                     /gene_synonym="4833423O18Rik; Tmtsp"
                     /inference="alignment:Splign:2.1.0"
     exon            1390..4432
                     /gene="Thsd1"
                     /gene_synonym="4833423O18Rik; Tmtsp"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2981..2986
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Thsd1"
                     /gene_synonym="4833423O18Rik; Tmtsp"
                     /note="hexamer: AATAAA"
     polyA_site      3002
                     /gene="Thsd1"
                     /gene_synonym="4833423O18Rik; Tmtsp"
                     /note="major polyA site"
     regulatory      3455..3460
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Thsd1"
                     /gene_synonym="4833423O18Rik; Tmtsp"
                     /note="hexamer: ATTAAA"
     polyA_site      3477
                     /gene="Thsd1"
                     /gene_synonym="4833423O18Rik; Tmtsp"
                     /note="major polyA site"
     regulatory      4404..4409
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Thsd1"
                     /gene_synonym="4833423O18Rik; Tmtsp"
                     /note="hexamer: AATATA"
     polyA_site      4432
                     /gene="Thsd1"
                     /gene_synonym="4833423O18Rik; Tmtsp"
ORIGIN      
        1 gggagcgtgc cggagctctg gacccgggcg ggcgtgcagc tgcgggacgg gagatgctcg
       61 gtcagtcacc ctgtggccgc tgaggctccc ctctcacttc cgccgtgcca gacagacccg
      121 acccgaggtg cttttggaaa aaaaaaaatc ccacaatttt ggtgtgagaa aggacatgag
      181 gagactacag gcctgggatc ttcccgatca gaatgaaacc catgttgaaa gacttttcaa
      241 atctcttgct ggtggtgctc tgtgactatg tcctcggaga agccgaatac ctcctcctcc
      301 aagagccagt ccatgtggca ctgagcgaca gaacggtgtc agtgggtttc cactacctca
      361 gtgacgtcaa cgggacactg aggaatgtgt ctgtcatgct gtgggaggcc aacaccaatc
      421 ggactcttac cactaagtac ctcctgacca accaggccca aggaacactc cagtttgaat
      481 gtttctactt caaagaggct ggtgactact ggtttgtaat gatcccggaa gtgacagaca
      541 atggcacgca agttccactc tgggagaaaa gtgcctttct gaaggtagaa tggcctgtct
      601 ttcacattga tttaaatagg acagccaagg cagcagaagg cacctttcaa gtgggtgttt
      661 ttaccaccca accgctctgc ctgtttcccg tggacaagcc agacatgctg gtggatgtta
      721 ttttcactga ccgtcttccg gaggcaagag caagtttggg acagccgctg gagatcagag
      781 ccagcaaaag gacaaaactc actcaaggtc agtgggtcga gtttggctgt gcaccggtag
      841 gggtggaagc ctacgttaca gtcatgctga ggctgttggg tcaagactca gtcattgctt
      901 ctacgggacc tattgacctg gctcaaaaat tcggatacaa attgatgatg gcaccggaag
      961 tcacgtgtga gtctgtgctg gaggtgatgg tactgccacc tccttgtgtc ttcgtccaag
     1021 gagtcctggc tgtttacaaa gaagccccca aacgcccgga ggagaggact ttccaggtgg
     1081 ctgaaaacag actgcccctg ggagagagga gaacggtgtt caactgcact ttatttgatg
     1141 tagggaagaa caaatactgt tttaactttg gaattgtgaa gaaaggccat ttttctgcaa
     1201 aggaatgcat gctaattcag agaaatatag aaacttgggg accatggcag ccgtggagcc
     1261 cgtgtagcac cacgtgcggg gatgctgtcc gagagcgtcg ccgcctgtgt gtcacttctt
     1321 tcccctccag acccagctgc tctggaatgt cctcagagac ctctccatgc tccctggagg
     1381 agtgtgctgt tttccggcca ccaggcccat cgcctgtttc accccaggac cctgtgaagt
     1441 ccaacaacgt ggtgaccgtc acagggatct ccctgtgcct gttcatcatc tttgccacgg
     1501 tgctcatcac tctctggagg aggtttggcc gagcccccaa atgcagcacg cccgttcgcc
     1561 acaactccat ccattcccct ggcttccgga agaactccga tgaagagaac atctgcgagc
     1621 tgagtgagcc tcgcggaagc ttctcggatg ccggtgacgg acctagggga agcccagggg
     1681 acacgggcat cccattgact tacaggtgca gtgcatcagc gcctcctgag gatgaggcct
     1741 cgggcagtga gagcttccag tccaacgctc agaagatcat cccgcccttg tttagctacc
     1801 gccttgccca gcagcagctg aaggagatga agaagaaagg gctgaccgag accaccaaag
     1861 tgtaccacgt atctcaaagc cccctgacag acactgtagt ggatgccacg gccagccctc
     1921 ccttagacct ggaatgcccc gaagaggctg cagcaagcaa gttccgaatc aaatctccat
     1981 ttctggacca gcctggggca ggtaccgggg aaaggcctcc ctccaggctg gatggcatcg
     2041 tgcctcctcc tggctgtgcg gtcagtccca gccagaccct gatccgaaag tcacagataa
     2101 ggtcgaccgg tggcagagat ggctcatcgg agaggtgcca ctccagaagt tccctcttca
     2161 ggaggactgc tagttttcat gaaaccaagc agtctcgccc tttccgggag aggagtttgt
     2221 cagccctgac tccccgccag gtccccgcct acagttccag gatgcggacc tgggaccaga
     2281 tggaggatag atgtcggcct cccagtcgaa gtacccacct gcttccagag agaccagagc
     2341 acttccaagg ggcaggtcgg accagcagtc ctttgggtcc actctccaaa tcctacactg
     2401 tggggcatcc caggaggaaa ccggacccag gggatcgtca ggccggattg gtggcaggag
     2461 ctgagaaaat ggagcctcac cgagctcaca ggggaccgtc ccccagtcac aggagtgcct
     2521 caaggaagca gtcttccccc attttcctca aagatagcta ccagaaagtc agtcagctta
     2581 gcccttctca cttcagaaaa gataaatgcc agagcttccc catccacccc gagttcgcct
     2641 tctatgacaa tacctctttc cgcctcaccg aggctgagca gagaatgctg gacctcccag
     2701 gatacttcgg ctccaacgaa gaggacgaaa ccacaagtac actcagtgtg gagaagttag
     2761 tgatctaggc taggaagcag cagggttcac ccagggtcct tcacccctct ggcaatatcc
     2821 cctgctcaca tactttctgt gggctgcact gtacaaccgt gggaattagt tcacccagat
     2881 tatatgtgtg cgcgcgcatg tggttttgat taatcaggaa gaagtggttt atgctgaatt
     2941 ttcctctctg ttatcccttt tctattggct tgttactact aataaaattt aaagaaggca
     3001 aatttatgat gcattttgaa cacgacactg gatgtttcca acttaggact ctgggatctt
     3061 ttcccaagag ggaggagtct ccaaatggct gatgtgtatg caaaccatta tgcgcagagc
     3121 ttgcatcttg cttccttgta aagtaggaac caaacacagg agcctaaaca tggggttgct
     3181 tcttttgaag tgtgttacca aggtaccaag tggcataaag cctttctgcc cagtggccta
     3241 aagactaatc agcatacacg taaatagcta caagctcaag gcgacctcca acaaccacac
     3301 ccatcctgcc tttagattga tttccttctt ttagagttta tcaggatgtc gggcagctgg
     3361 ggaggtttca gataggggga aatcacaaat gtaaacacgg gagtttcaaa agttcatatt
     3421 tgttcaactg tcacttgtga ctttttgttt ttaaattaaa atgtgcttcc tttggaaccg
     3481 ttctcagtga tgactgcctg ctcttcagga acaggacggg tggattgtcc agagccctct
     3541 gacagctgct actcgtggat gaaaggagag ccaggcttgc tactctccag tttctttatt
     3601 tatctcacca gaaagctttc ttctttatcc aagttcacag gcctgtgggc cagcctttga
     3661 cacagagggc acatgatcgc ttgttccaga cttcccagag atttacccag caccgtcagt
     3721 ctgagaccaa aacagctttg gggaagtggg aattcatgag gaacactatg agctgagagc
     3781 cttcctgtta agtgatgctc actgtctacc caggccagtg ttctggggct gtcactaaca
     3841 gtgacacccc ccccccacct ccgagggaga aaagatgctt tatcacagtt tttaatgcgt
     3901 gagttataat tttcagtaaa cagattttct gtatcgtacc tccatgtctc catgttctag
     3961 tcccggtatc tgctatagaa tggaggctca ataaagacag tgagtggcca gctagtacaa
     4021 tgcctttaaa ccagttgtgg ttgaattaga atgattttta ataatacttt acattgtaga
     4081 tcattataaa ggaaatgtgt ttatgagaca ttatgagatt tcagttcata caggtctcac
     4141 aaacatgttt tttgttttgt tttattttgt tttgttttgt tttttggtgt caccaattcg
     4201 tgtgttccca atcatcctgc gaacaatcag ttatgcttgg ccagcgtttt gctggctccg
     4261 tgtttcagat tatcttagta tatgtaactt catggatctg ggtaagctcg acacacttag
     4321 atgtttattg tctgtgtccc caccagttgt gactccctgc aatgtgtctg tgttcacctt
     4381 gctgaaaaga agtgttcttt ctaaatatag tttttaatct aaaaatttaa aa
//
