LOCUS       NM_001173505            2262 bp    mRNA    linear   ROD 11-FEB-2025
DEFINITION  Mus musculus thrombopoietin (Thpo), transcript variant 2, mRNA.
ACCESSION   NM_001173505
VERSION     NM_001173505.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2262)
  AUTHORS   Brandao,W., Jain,N., Yin,Z., Kleemann,K.L., Carpenter,M., Bao,X.,
            Serrano,J.R., Tycksen,E., Durao,A., Barry,J.L., Baufeld,C.,
            Guneykaya,D., Zhang,X., Litvinchuk,A., Jiang,H., Rosenzweig,N.,
            Pitts,K.M., Aronchik,M., Yahya,T., Cao,T., Takahashi,M.K.,
            Krishnan,R., Davtyan,H., Ulrich,J.D., Blurton-Jones,M., Ilin,I.,
            Weiner,H.L., Holtzman,D.M. and Butovsky,O.
  TITLE     Inhaled xenon modulates microglia and ameliorates disease in mouse
            models of amyloidosis and tauopathy
  JOURNAL   Sci Transl Med 17 (781), eadk3690 (2025)
   PUBMED   39813317
REFERENCE   2  (bases 1 to 2262)
  AUTHORS   Sun,Y., Tong,H., Chu,X., Li,Y., Zhang,J., Ding,Y., Zhang,S.,
            Gui,X., Chen,C., Xu,M., Li,Z., Gardiner,E.E., Andrews,R.K.,
            Zeng,L., Xu,K. and Qiao,J.
  TITLE     Notch1 regulates hepatic thrombopoietin production
  JOURNAL   Blood 143 (26), 2778-2790 (2024)
   PUBMED   38603632
  REMARK    GeneRIF: Notch1 regulates hepatic thrombopoietin production.
REFERENCE   3  (bases 1 to 2262)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   4  (bases 1 to 2262)
  AUTHORS   Sarson-Lawrence,K.T.G., Hardy,J.M., Iaria,J., Stockwell,D.,
            Behrens,K., Saiyed,T., Tan,C., Jebeli,L., Scott,N.E., Dite,T.A.,
            Nicola,N.A., Leis,A.P., Babon,J.J. and Kershaw,N.J.
  TITLE     Cryo-EM structure of the extracellular domain of murine
            Thrombopoietin Receptor in complex with Thrombopoietin
  JOURNAL   Nat Commun 15 (1), 1135 (2024)
   PUBMED   38326297
  REMARK    GeneRIF: Cryo-EM structure of the extracellular domain of murine
            Thrombopoietin Receptor in complex with Thrombopoietin.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2262)
  AUTHORS   Wu,Xl., Nakayama,M. and Adamson,J.W.
  TITLE     Identification of five new isoforms of murine thrombopoietin mRNA
  JOURNAL   Biochem Biophys Res Commun 276 (1), 137-143 (2000)
   PUBMED   11006096
REFERENCE   6  (bases 1 to 2262)
  AUTHORS   Yan,X.Q., Lacey,D., Fletcher,F., Hartley,C., McElroy,P., Sun,Y.,
            Xia,M., Mu,S., Saris,C., Hill,D., Hawley,R.G. and McNiece,I.K.
  TITLE     Chronic exposure to retroviral vector encoded MGDF (mpl-ligand)
            induces lineage-specific growth and differentiation of
            megakaryocytes in mice
  JOURNAL   Blood 86 (11), 4025-4033 (1995)
   PUBMED   7492757
REFERENCE   7  (bases 1 to 2262)
  AUTHORS   Wada,T., Nagata,Y., Nagahisa,H., Okutomi,K., Ha,S.H., Ohnuki,T.,
            Kanaya,T., Matsumura,M. and Todokoro,K.
  TITLE     Characterization of the truncated thrombopoietin variants
  JOURNAL   Biochem Biophys Res Commun 213 (3), 1091-1098 (1995)
   PUBMED   7654225
REFERENCE   8  (bases 1 to 2262)
  AUTHORS   Pallard,C., Gouilleux,F., Benit,L., Cocault,L., Souyri,M., Levy,D.,
            Groner,B., Gisselbrecht,S. and Dusanter-Fourt,I.
  TITLE     Thrombopoietin activates a STAT5-like factor in hematopoietic cells
  JOURNAL   EMBO J 14 (12), 2847-2856 (1995)
   PUBMED   7796811
REFERENCE   9  (bases 1 to 2262)
  AUTHORS   Chang,M.S., Hsu,R.Y., McNinch,J., Copeland,N.G. and Jenkins,N.A.
  TITLE     The gene for murine megakaryocyte growth and development factor
            (thrombopoietin, Thpo) is located on mouse chromosome 16
  JOURNAL   Genomics 26 (3), 636-637 (1995)
   PUBMED   7607698
REFERENCE   10 (bases 1 to 2262)
  AUTHORS   Gurney,A.L., Kuang,W.J., Xie,M.H., Malloy,B.E., Eaton,D.L. and de
            Sauvage,F.J.
  TITLE     Genomic structure, chromosomal localization, and conserved
            alternative splice forms of thrombopoietin
  JOURNAL   Blood 85 (4), 981-988 (1995)
   PUBMED   7849319
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AI314551.1, CT010490.10 and
            AK047512.1.
            
            Summary: This gene encodes a humoral growth factor necessary for
            megakaryocyte proliferation and maturation, as well as for
            thrombopoiesis. The encoded protein is a ligand for the product of
            the myeloproliferative leukemia virus oncogene. Alternative
            splicing results in multiple transcript variants. [provided by
            RefSeq, Mar 2010].
            
            Transcript Variant: This variant (2) encodes the longest isoform
            (1, also known as Tpo1). Both variants 1 and 2 encode the same
            isoform 1.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: L34169.1, SRR6116558.22184.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164131 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-66                AI314551.1         1-66
            67-96               CT010490.10        75743-75772         c
            97-250              CT010490.10        74304-74457         c
            251-378             CT010490.10        73959-74086         c
            379-465             CT010490.10        73622-73708         c
            466-633             CT010490.10        71546-71713         c
            634-1550            CT010490.10        70406-71322         c
            1551-2262           AK047512.1         1706-2417
FEATURES             Location/Qualifiers
     source          1..2262
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="16"
                     /map="16 12.51 cM"
     gene            1..2262
                     /gene="Thpo"
                     /gene_synonym="Mgdf; Ml; Mpllg; Tpo"
                     /note="thrombopoietin"
                     /db_xref="GeneID:21832"
                     /db_xref="MGI:MGI:101875"
     exon            1..96
                     /gene="Thpo"
                     /gene_synonym="Mgdf; Ml; Mpllg; Tpo"
                     /inference="alignment:Splign:2.1.0"
     exon            97..250
                     /gene="Thpo"
                     /gene_synonym="Mgdf; Ml; Mpllg; Tpo"
                     /inference="alignment:Splign:2.1.0"
     CDS             238..1308
                     /gene="Thpo"
                     /gene_synonym="Mgdf; Ml; Mpllg; Tpo"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 2; C-mpl ligand; megakaryocyte colony-stimulating
                     factor; megakaryocyte growth and development factor;
                     myeloproliferative leukemia virus oncogene ligand"
                     /codon_start=1
                     /product="thrombopoietin isoform 1 precursor"
                     /protein_id="NP_001166976.1"
                     /db_xref="CCDS:CCDS37292.1"
                     /db_xref="GeneID:21832"
                     /db_xref="MGI:MGI:101875"
                     /translation="MELTDLLLAAMLLAVARLTLSSPVAPACDPRLLNKLLRDSHLLH
                     SRLSQCPDVDPLSIPVLLPAVDFSLGEWKTQTEQSKAQDILGAVSLLLEGVMAARGQL
                     EPSCLSSLLGQLSGQVRLLLGALQGLLGTQLPLQGRTTAHKDPNALFLSLQQLLRGKV
                     RFLLLVEGPTLCVRRTLPTTAVPSSTSQLLTLNKFPNRTSGLLETNFSVTARTAGPGL
                     LSRLQGFRVKITPGQLNQTSRSPVQISGYLNRTHGPVNGTHGLFAGTSLQTLEASDIS
                     PGAFNKGSLAFNLQGGLPPSPSLAPDGHTPFPPSPALPTTHGSPPQLHPLFPDPSTTM
                     PNSTAPHPVTMYPHPRNLSQET"
     exon            251..378
                     /gene="Thpo"
                     /gene_synonym="Mgdf; Ml; Mpllg; Tpo"
                     /inference="alignment:Splign:2.1.0"
     exon            379..465
                     /gene="Thpo"
                     /gene_synonym="Mgdf; Ml; Mpllg; Tpo"
                     /inference="alignment:Splign:2.1.0"
     exon            466..633
                     /gene="Thpo"
                     /gene_synonym="Mgdf; Ml; Mpllg; Tpo"
                     /inference="alignment:Splign:2.1.0"
     exon            634..2262
                     /gene="Thpo"
                     /gene_synonym="Mgdf; Ml; Mpllg; Tpo"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1531..1536
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Thpo"
                     /gene_synonym="Mgdf; Ml; Mpllg; Tpo"
                     /note="hexamer: TATAAA"
     polyA_site      1551
                     /gene="Thpo"
                     /gene_synonym="Mgdf; Ml; Mpllg; Tpo"
                     /note="major polyA site"
     regulatory      1768..1773
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Thpo"
                     /gene_synonym="Mgdf; Ml; Mpllg; Tpo"
                     /note="hexamer: GATAAA"
     polyA_site      1794
                     /gene="Thpo"
                     /gene_synonym="Mgdf; Ml; Mpllg; Tpo"
     regulatory      1863..1868
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Thpo"
                     /gene_synonym="Mgdf; Ml; Mpllg; Tpo"
                     /note="hexamer: AATAAA"
     polyA_site      1895
                     /gene="Thpo"
                     /gene_synonym="Mgdf; Ml; Mpllg; Tpo"
ORIGIN      
        1 gaggaggggc tgtaccccaa ccagtgtctt cctacctact tgttccccag agtgctgcct
       61 gctgtgcacc tgggtcctga ggcccttctc cacccggaca gagtccttgg cccacctctc
      121 tcccacccga ctctgccgaa agaagcacag aagctcaagc cgcctccatg gccccaggaa
      181 agattcaggg gagaggcccc atacagggag ccacttcagt tagacaccct ggccagaatg
      241 gagctgactg atttgctcct ggcggccatg cttcttgcag tggcaagact aactctgtcc
      301 agccccgtag ctcctgcctg tgaccccaga ctcctaaata aactgctgcg tgactcccac
      361 ctccttcaca gccgactgag tcagtgtccc gacgtcgacc ctttgtctat ccctgttctg
      421 ctgcctgctg tggactttag cctgggagaa tggaaaaccc agacggaaca gagcaaggca
      481 caggacattc taggggcagt gtcccttcta ctggagggag tgatggcagc acgaggacag
      541 ttggaaccct cctgcctctc atccctcctg ggacagcttt ctgggcaggt tcgcctcctc
      601 ttgggggccc tgcagggcct cctaggaacc cagcttcctc tacagggcag gaccacagct
      661 cacaaggacc ccaatgccct cttcttgagc ttgcaacaac tgcttcgggg aaaggtgcgc
      721 ttcctgcttc tggtagaagg tcccaccctc tgtgtcagac ggaccctgcc aaccacagct
      781 gtcccaagca gtacttctca actcctcaca ctaaacaagt tcccaaacag gacttctgga
      841 ttgttggaga cgaacttcag tgtcacagcc agaactgctg gccctggact tctgagcagg
      901 cttcagggat tcagagtcaa gattactcct ggtcagctaa atcaaacctc caggtcccca
      961 gtccaaatct ctggatacct gaacaggaca cacggacctg tgaatggaac tcatgggctc
     1021 tttgctggaa cctcacttca gaccctggaa gcctcagaca tctcgcccgg agctttcaac
     1081 aaaggctccc tggcattcaa cctccagggt ggacttcctc cttctccaag ccttgctcct
     1141 gatggacaca cacccttccc tccttcacct gccttgccca ccacccatgg atctccaccc
     1201 cagctccacc ccctgtttcc tgacccttcc accaccatgc ctaactctac cgcccctcat
     1261 ccagtcacaa tgtaccctca tcccaggaat ttgtctcagg aaacatagcg cgggcactgg
     1321 cccagtgagc gtctgcagct tctctcgggg acaagcttcc ccaggaaggc tgagaggcag
     1381 ctgcatctgc tccagatgtt ctgctttcac ctaaaaggcc ctggggaagg gatacacagc
     1441 actggagatt gtaaaatttt aggagctatt tttttttaac ctatcagcaa tattcatcag
     1501 agcagctagc gatctttggt ctattttcgg tataaatttg aaaatcacta attctctata
     1561 tgtgctttca tacattaagt ctgcaaatgc ctaggcaggt cttgcctttt aacccaggta
     1621 gatgctacac catatcagaa aacaaaaggg aactttcctt tgcttcaagt ttaagccttc
     1681 ccgcgcctcc aacaccccca caatgccctc cttcccttca ctgtaattct cagtgagact
     1741 gtatggtcct cgagatatac tgctcttgat aaagaattaa caggctatca cttagaaaga
     1801 ctgtccctac ttaagagaca aaactgaacc tgtaagagaa taactgggag cacccaaaag
     1861 ccaataaaaa tcagggacca atgttcttca ctggggcaac agagctcctg gaccctgcct
     1921 cccaagaaag ctaacaggaa gcctgggagc tccacacccc aggtaaggct gtgcagctgg
     1981 ctcagtaaag agcagacttg gatgtggcag ctgagcaaag agcatcagca gctcagcagg
     2041 ggctcagcca ggcctgggct cctgcttccc tcctgtggag gtcaggcgga agtgcaggaa
     2101 gtggcaagag gcaggctcct cggctcacac agcaggacaa gcacagagcg cttgaaggct
     2161 ctttataatt tccacaaatg cacctaaaaa gcagccctgt gtggccactc caaactttat
     2221 tggaaccctc ccaaatgaag gcagacttat aggactttcc aa
//
