LOCUS       NM_022653               2585 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus thimet oligopeptidase 1 (Thop1), mRNA.
ACCESSION   NM_022653
VERSION     NM_022653.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2585)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 2585)
  AUTHORS   Gewehr,M.C.F., Teixeira,A.A.S., Santos,B.A.C., Biondo,L.A.,
            Gozzo,F.C., Cordibello,A.M., Eichler,R.A.S., Reckziegel,P., Da
            Silva,R.N.O., Dos Santos,N.B., Camara,N.O.S., Castoldi,A.,
            Barreto-Chaves,M.L.M., Dale,C.S., Senger,N., Lima,J.D.C.C.,
            Seelaender,M.C.L., Inada,A.C., Akamine,E.H., Castro,L.M.,
            Rodrigues,A.C., Neto,J.C.R. and Ferro,E.S.
  TITLE     The Relevance of Thimet Oligopeptidase in the Regulation of Energy
            Metabolism and Diet-Induced Obesity
  JOURNAL   Biomolecules 10 (2), 321 (2020)
   PUBMED   32079362
  REMARK    GeneRIF: The Relevance of Thimet Oligopeptidase in the Regulation
            of Energy Metabolism and Diet-Induced Obesity.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2585)
  AUTHORS   Santos,N.B.D., Franco,R.D., Camarini,R., Munhoz,C.D.,
            Eichler,R.A.S., Gewehr,M.C.F., Reckziegel,P., Llanos,R.P.,
            Dale,C.S., Silva,V.R.O.D., Borges,V.F., Lima,B.H.F., Cunha,F.Q.,
            Visniauskas,B., Chagas,J.R., Tufik,S., Peres,F.F., Abilio,V.C.,
            Florio,J.C., Iwai,L.K., Rioli,V., Presoto,B.C., Guimaraes,A.O.,
            Pesquero,J.B., Bader,M., Castro,L.M. and Ferro,E.S.
  TITLE     Thimet Oligopeptidase (EC 3.4.24.15) Key Functions Suggested by
            Knockout Mice Phenotype Characterization
  JOURNAL   Biomolecules 9 (8), 382 (2019)
   PUBMED   31431000
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2585)
  AUTHORS   Gamrekelashvili,J., Kapanadze,T., Han,M., Wissing,J., Ma,C.,
            Jaensch,L., Manns,M.P., Armstrong,T., Jaffee,E., White,A.O.,
            Citrin,D.E., Korangy,F. and Greten,T.F.
  TITLE     Peptidases released by necrotic cells control CD8+ T cell
            cross-priming
  JOURNAL   J Clin Invest 123 (11), 4755-4768 (2013)
   PUBMED   24216478
  REMARK    GeneRIF: the cellular peptidases dipeptidyl peptidase 3 (DPP-3) and
            thimet oligopeptidase 1 (TOP-1), both of which are present in
            nonimmunogenic necrotic cells, eliminated proteasomal degradation
            products and blocked Ag cross-presentation
REFERENCE   5  (bases 1 to 2585)
  AUTHORS   Cyr,N.E., Kua,L.H., Bruce,L.A., Chadwick,J.G., Tetel,M.J. and
            Wolfson,A.J.
  TITLE     Nuclear Thimet oligopeptidase is coexpressed with oestrogen
            receptor alpha in hypothalamic cells and regulated by oestradiol in
            female mice
  JOURNAL   J Neuroendocrinol 22 (8), 936-943 (2010)
   PUBMED   20456597
  REMARK    GeneRIF: Data show that Thimet oligopeptidase (TOP) is coexpressed
            with estrogen receptor alpha, and estradiol regulates TOP
            expression in a brain region-specific manner in female mice.
REFERENCE   6  (bases 1 to 2585)
  AUTHORS   Carninci,P., Waki,K., Shiraki,T., Konno,H., Shibata,K., Itoh,M.,
            Aizawa,K., Arakawa,T., Ishii,Y., Sasaki,D., Bono,H., Kondo,S.,
            Sugahara,Y., Saito,R., Osato,N., Fukuda,S., Sato,K., Watahiki,A.,
            Hirozane-Kishikawa,T., Nakamura,M., Shibata,Y., Yasunishi,A.,
            Kikuchi,N., Yoshiki,A., Kusakabe,M., Gustincich,S., Beisel,K.,
            Pavan,W., Aidinis,V., Nakagawara,A., Held,W.A., Iwata,H., Kono,T.,
            Nakauchi,H., Lyons,P., Wells,C., Hume,D.A., Fagiolini,M.,
            Hensch,T.K., Brinkmeier,M., Camper,S., Hirota,J., Mombaerts,P.,
            Muramatsu,M., Okazaki,Y., Kawai,J. and Hayashizaki,Y.
  TITLE     Targeting a complex transcriptome: the construction of the mouse
            full-length cDNA encyclopedia
  JOURNAL   Genome Res 13 (6B), 1273-1289 (2003)
   PUBMED   12819125
REFERENCE   7  (bases 1 to 2585)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
REFERENCE   8  (bases 1 to 2585)
  AUTHORS   Piao,Y., Ko,N.T., Lim,M.K. and Ko,M.S.
  TITLE     Construction of long-transcript enriched cDNA libraries from
            submicrogram amounts of total RNAs by a universal PCR amplification
            method
  JOURNAL   Genome Res 11 (9), 1553-1558 (2001)
   PUBMED   11544199
REFERENCE   9  (bases 1 to 2585)
  AUTHORS   Tullai,J.W., Cummins,P.M., Pabon,A., Roberts,J.L., Lopingco,M.C.,
            Shrimpton,C.N., Smith,A.I., Martignetti,J.A., Ferro,E.S. and
            Glucksman,M.J.
  TITLE     The neuropeptide processing enzyme EC 3.4.24.15 is modulated by
            protein kinase A phosphorylation
  JOURNAL   J Biol Chem 275 (47), 36514-36522 (2000)
   PUBMED   10969067
REFERENCE   10 (bases 1 to 2585)
  AUTHORS   Puttagunta,R., Gordon,L.A., Meyer,G.E., Kapfhamer,D.,
            Lamerdin,J.E., Kantheti,P., Portman,K.M., Chung,W.K., Jenne,D.E.,
            Olsen,A.S. and Burmeister,M.
  TITLE     Comparative maps of human 19p13.3 and mouse chromosome 10 allow
            identification of sequences at evolutionary breakpoints
  JOURNAL   Genome Res 10 (9), 1369-1380 (2000)
   PUBMED   10984455
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BY774276.1, CF163503.1, AK028609.1 and CN723672.1.
            
            On Jun 13, 2009 this sequence version replaced NM_022653.3.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK028609.1, BC031722.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-19                BY774276.1         2-20
            20-67               CF163503.1         1-48
            68-573              AK028609.1         2-507
            574-1038            CN723672.1         119-583
            1039-2585           AK028609.1         973-2519
FEATURES             Location/Qualifiers
     source          1..2585
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="10"
                     /map="10 39.72 cM"
     gene            1..2585
                     /gene="Thop1"
                     /gene_synonym="EP24.15"
                     /note="thimet oligopeptidase 1"
                     /db_xref="GeneID:50492"
                     /db_xref="MGI:MGI:1354165"
     exon            1..196
                     /gene="Thop1"
                     /gene_synonym="EP24.15"
                     /inference="alignment:Splign:2.1.0"
     CDS             181..2244
                     /gene="Thop1"
                     /gene_synonym="EP24.15"
                     /EC_number="3.4.24.15"
                     /note="endopeptidase 24.15"
                     /codon_start=1
                     /product="thimet oligopeptidase"
                     /protein_id="NP_073144.3"
                     /db_xref="CCDS:CCDS24042.1"
                     /db_xref="GeneID:50492"
                     /db_xref="MGI:MGI:1354165"
                     /translation="MKPPAACAGDVVDAASPASTVNHLRWDLSAQQIRALTTQLIEQT
                     KCVYDRVGAQNFEDVSYESTLKALADVEVTYTVQRNILDFPQHVSPCKDIRAASTEAD
                     KKLSEFDVEMSMRQDVYQRVVWLQEKTPKDSLKPEAARYLERLIKLGRRNGLHLPQDT
                     QEKIKNIKKRLSLLCIDFNKNLNEDTTFLPFTREELGGLPEDFLSSLEKAEDGKLKVT
                     LKYPHYFPLLKKCHVPETRRLLEEAFNCRCKEENCAILKELVSLRAQKSSLLGFHTHA
                     DYVLEMNMAKTSQTVATFLDELAQKLKPLGEQERAVILELKEAECAKRGLPFDGRIHA
                     WDMRYYMNQVEETRYRVDQNLLKEYFPMQVVTRGLLAIYQELLGLTFTLEEGAAAWHE
                     DVRLYSVRDAASGEEIGKFYLDLYPREGKYGHAACFGLQPGCLRQDGSRQLAVAAMVA
                     NFTKPTPDAPSLLQHDEVETYFHEFGHVMHQLCSQAEFAMFSGTHVERDFVEAPSQML
                     ENWVWEKEPLMRMSQHYRTGSEAPQDLLEKLIKSRQANAGLFNLRQIVLAKVDQVLHT
                     QTDADPAEEYARLCQEILGVPATPGTNMPATFGHLAGGYDAQYYGYLWSEVYSMDMFH
                     TRFKQEGVLSPKVGMDYRTSILRPGGSEDASAMLKQFLGRDPKQDAFLLSKGLQVEGS
                     EAPAC"
     exon            197..409
                     /gene="Thop1"
                     /gene_synonym="EP24.15"
                     /inference="alignment:Splign:2.1.0"
     exon            410..558
                     /gene="Thop1"
                     /gene_synonym="EP24.15"
                     /inference="alignment:Splign:2.1.0"
     exon            559..666
                     /gene="Thop1"
                     /gene_synonym="EP24.15"
                     /inference="alignment:Splign:2.1.0"
     exon            667..769
                     /gene="Thop1"
                     /gene_synonym="EP24.15"
                     /inference="alignment:Splign:2.1.0"
     exon            770..930
                     /gene="Thop1"
                     /gene_synonym="EP24.15"
                     /inference="alignment:Splign:2.1.0"
     exon            931..1066
                     /gene="Thop1"
                     /gene_synonym="EP24.15"
                     /inference="alignment:Splign:2.1.0"
     exon            1067..1433
                     /gene="Thop1"
                     /gene_synonym="EP24.15"
                     /inference="alignment:Splign:2.1.0"
     exon            1434..1635
                     /gene="Thop1"
                     /gene_synonym="EP24.15"
                     /inference="alignment:Splign:2.1.0"
     exon            1636..1822
                     /gene="Thop1"
                     /gene_synonym="EP24.15"
                     /inference="alignment:Splign:2.1.0"
     exon            1823..1951
                     /gene="Thop1"
                     /gene_synonym="EP24.15"
                     /inference="alignment:Splign:2.1.0"
     exon            1952..2088
                     /gene="Thop1"
                     /gene_synonym="EP24.15"
                     /inference="alignment:Splign:2.1.0"
     exon            2089..2585
                     /gene="Thop1"
                     /gene_synonym="EP24.15"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2425..2430
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Thop1"
                     /gene_synonym="EP24.15"
                     /note="hexamer: ATTAAA"
     polyA_site      2445
                     /gene="Thop1"
                     /gene_synonym="EP24.15"
                     /note="major polyA site"
ORIGIN      
        1 agctgccttc gcgcgcacgc gccttcctag cgtgctcgga gctcgcgggc ggcgggcgcc
       61 tcagtctgag agccacagca gaggaactgg ctcaggtgag gcgagctccg aggagatccg
      121 gaccgcacag gcgtgaggac cagaggaggg ccgtcgccgc ctcgcgcgca gccgaccgcc
      181 atgaagcccc ccgcagcctg tgctggggac gtggtggatg cagcatcccc agcctccacc
      241 gtgaatcatc ttcgctggga tctgagcgca cagcagatca gggctctaac cacgcagctc
      301 atcgagcaga ccaagtgtgt gtacgaccga gtgggtgctc agaacttcga ggatgtgtcg
      361 tatgagagca cactgaaggc actggctgac gtggaggtca cctacacagt gcagaggaac
      421 attctcgact tcccccaaca cgtgtccccg tgcaaggaca tccgtgcagc cagcacagag
      481 gctgacaaga agctctcgga gtttgatgta gagatgagca tgagacagga cgtgtaccag
      541 agggtcgtgt ggctgcagga gaaaaccccg aaagattccc tgaagcctga agcagctcgc
      601 tacctggagc ggctcatcaa gctgggccgg agaaacggcc tccacttacc tcaggacaca
      661 caagagaaga tcaagaacat caagaagagg ctgagcctgc tgtgcatcga cttcaacaag
      721 aacctcaacg aggacaccac cttcctgccc ttcacaagag aggagctggg tgggctcccg
      781 gaggactttc tgagctctct ggagaaggca gaggacggca agctgaaggt caccctcaag
      841 taccctcact acttcccgct gttgaagaag tgccatgtgc cggagacacg acgcctgttg
      901 gaggaggcct tcaactgtcg ctgcaaggag gagaactgtg ccattctgaa ggagctagtg
      961 tccctgcgag cacagaagtc cagcctgttg gggttccaca cacacgcgga ctacgtcctg
     1021 gagatgaaca tggccaagac cagtcaaaca gtagccacct tcctagatga gctggcacag
     1081 aagctgaagc ccctgggtga gcaggagcgc gcggtcatcc tggagctgaa ggaggcagag
     1141 tgcgccaagc ggggactgcc attcgacggg cgcatccacg catgggacat gcgctactac
     1201 atgaaccagg tggaggagac tcgctatcgt gtggaccaga acctgctgaa ggagtacttc
     1261 cccatgcagg tggtcacacg tgggctgctg gccatctacc aagagctgct ggggctgacc
     1321 ttcacgctgg aggaaggtgc cgctgcctgg cacgaggacg tgcggctcta ctcagtgcgg
     1381 gacgccgcct ctggagagga gatcggcaag ttctacctcg acctataccc cagggaaggg
     1441 aaatatggcc atgcagcctg ctttggcctg cagcctggct gcctacggca ggatggcagc
     1501 cggcagctgg ccgtcgcagc catggtggcc aatttcacga agcccacacc tgacgcgcca
     1561 tccctgctgc agcatgatga ggtggagacc tacttccacg agttcgggca cgtcatgcac
     1621 cagctgtgct cgcaggcgga gtttgccatg ttcagtggga cccacgtgga gcgggacttt
     1681 gtggaggcgc cgtcccagat gctggagaac tgggtgtggg agaaggagcc cctgatgcgc
     1741 atgtctcagc actaccgcac gggcagcgag gcccctcagg acctcctgga gaagctcatc
     1801 aagtctcgcc aggccaatgc aggtctcttc aacctgcggc agattgtgct tgccaaggtg
     1861 gatcaggtcc tgcacacaca gacagacgca gacccggctg aggaatatgc ccggctctgc
     1921 caagagatcc tcggggtgcc agccacacca ggtaccaaca tgccagccac tttcggccac
     1981 ctagctggcg gctatgatgc tcagtactat ggctacttat ggagcgaagt gtactcgatg
     2041 gacatgttcc acacacgctt caagcaagag ggtgtgctta gccccaaggt tggcatggac
     2101 tacaggacca gcatcctgag gccagggggc tctgaggacg ccagcgccat gctgaagcag
     2161 ttcctgggcc gcgaccccaa gcaggacgcc ttcctcctga gcaagggtct gcaggtggag
     2221 ggcagcgagg cgcctgcgtg ctgaccggtc cccagctcgc cgccctgtgc cttagcccag
     2281 cctgctgggc agctcagcaa cccttgggcg tacagatgag cttggggaag tttccatcct
     2341 tgtcccaccc cggctccagg tcccctggcc tcaggctgcc cagtggtctc cttgcactag
     2401 cccctcccca acctgatgtt ggacattaaa ctccacacag gggcaccctg cctggtcctc
     2461 tctctgtact gctctgcccc aggcttggcc agctacttcc tcactggcat catggtgtga
     2521 catgtgatag tctagccgtt ggagggtgct aagaactggt gccagggtca cctgcgcaat
     2581 gtgtc
//
