LOCUS       NM_028597               2326 bp    mRNA    linear   ROD 04-OCT-2024
DEFINITION  Mus musculus THO complex 3 (Thoc3), mRNA.
ACCESSION   NM_028597
VERSION     NM_028597.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2326)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 2326)
  AUTHORS   Bhattacharjee,R., Jolly,L.A., Corbett,M.A., Wee,I.C., Rao,S.R.,
            Gardner,A.E., Ritchie,T., van Hugte,E.J.H., Ciptasari,U., Piltz,S.,
            Noll,J.E., Nazri,N., van Eyk,C.L., White,M., Fornarino,D.,
            Poulton,C., Baynam,G., Collins-Praino,L.E., Snel,M.F., Nadif
            Kasri,N., Hemsley,K.M., Thomas,P.Q., Kumar,R. and Gecz,J.
  TITLE     Compromised transcription-mRNA export factor THOC2 causes R-loop
            accumulation, DNA damage and adverse neurodevelopment
  JOURNAL   Nat Commun 15 (1), 1210 (2024)
   PUBMED   38331934
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2326)
  AUTHORS   Wang,L., Miao,Y.L., Zheng,X., Lackford,B., Zhou,B., Han,L., Yao,C.,
            Ward,J.M., Burkholder,A., Lipchina,I., Fargo,D.C., Hochedlinger,K.,
            Shi,Y., Williams,C.J. and Hu,G.
  TITLE     The THO complex regulates pluripotency gene mRNA export and
            controls embryonic stem cell self-renewal and somatic cell
            reprogramming
  JOURNAL   Cell Stem Cell 13 (6), 676-690 (2013)
   PUBMED   24315442
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC165145.2.
            
            On Jul 22, 2009 this sequence version replaced NM_028597.2.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK010668.1, BC019603.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849381, SAMN01164135
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-300               AC165145.2         105030-105329       c
            301-457             AC165145.2         104315-104471       c
            458-662             AC165145.2         102669-102873       c
            663-824             AC165145.2         100171-100332       c
            825-925             AC165145.2         99961-100061        c
            926-2326            AC165145.2         95326-96726         c
FEATURES             Location/Qualifiers
     source          1..2326
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="13"
                     /map="13 28.69 cM"
     gene            1..2326
                     /gene="Thoc3"
                     /gene_synonym="2410044K02Rik"
                     /note="THO complex 3"
                     /db_xref="GeneID:73666"
                     /db_xref="MGI:MGI:1920916"
     exon            1..300
                     /gene="Thoc3"
                     /gene_synonym="2410044K02Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             34..1089
                     /gene="Thoc3"
                     /gene_synonym="2410044K02Rik"
                     /note="tho3"
                     /codon_start=1
                     /product="THO complex subunit 3"
                     /protein_id="NP_082873.2"
                     /db_xref="CCDS:CCDS26527.1"
                     /db_xref="GeneID:73666"
                     /db_xref="MGI:MGI:1920916"
                     /translation="MAAPAAVLGPSALGQSGPGSMAPWCSVSSGPSRYVLGMQELFRG
                     HSKTREFPAHSAKVHSVAWSCDGRRLASGSFDKTASVFLLEKDRLVKENNYRGHGDSV
                     DQLCWHPSNPDLFVTASGDKTIRIWDVRTTKCIATVNTKGENINICWSPDGQTIAVGN
                     KDDVVTFIDAKTHRSKAEEQFKFEVNEISWNNDNNMFFLTNGNGCINILSYPELKPVQ
                     SINAHPSNCICIKFDPMGKYFATGSADALVSLWDVDELVCVRCFSRLDWPVRTLSFSH
                     DGKMLASASEDHFIDIAEVETGDKLWEVQCESPTFTVAWHPKRPLLAFACDDKDGKYD
                     SSREAGTVKLFGLPNDS"
     exon            301..457
                     /gene="Thoc3"
                     /gene_synonym="2410044K02Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            458..662
                     /gene="Thoc3"
                     /gene_synonym="2410044K02Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            663..824
                     /gene="Thoc3"
                     /gene_synonym="2410044K02Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            825..925
                     /gene="Thoc3"
                     /gene_synonym="2410044K02Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            926..2326
                     /gene="Thoc3"
                     /gene_synonym="2410044K02Rik"
                     /inference="alignment:Splign:2.1.0"
     polyA_site      1346
                     /gene="Thoc3"
                     /gene_synonym="2410044K02Rik"
     regulatory      2307..2312
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Thoc3"
                     /gene_synonym="2410044K02Rik"
                     /note="hexamer: AATAAA"
     polyA_site      2326
                     /gene="Thoc3"
                     /gene_synonym="2410044K02Rik"
                     /note="major polyA site"
ORIGIN      
        1 agcttccggc gcgcggtccg tgaggaggga ggcatggcgg cgcccgctgc ggtcctgggc
       61 ccctcggccc tcggccagag cggccccggc tcgatggcgc cctggtgctc agtgagcagt
      121 ggcccgtctc gttacgtgct cgggatgcag gagctcttcc gaggccacag caagacgcgc
      181 gaattcccgg cgcacagtgc taaggtgcac tcggtcgcct ggagctgcga tggccgtcgc
      241 ttggcctcgg ggtccttcga caagactgcc agcgtgttct tgctggagaa ggaccggctg
      301 gtcaaagaaa acaactaccg gggccacgga gatagtgtgg accagctttg ttggcaccca
      361 agcaaccctg acctctttgt caccgcatct ggagataaaa ccattcgcat ctgggatgtg
      421 aggactacaa aatgcatcgc cactgtgaat acgaaagggg agaacattaa catctgttgg
      481 agtcccgacg ggcagaccat cgctgtgggc aacaaggacg acgttgtgac tttcattgac
      541 gccaagacac accgctccaa agcggaggag cagttcaagt ttgaggtcaa tgagatctca
      601 tggaacaacg acaacaacat gttcttcctg accaacggca atggttgcat caacatcctc
      661 agctacccag aattgaagcc tgtgcagtcc atcaatgccc atccttctaa ctgcatctgt
      721 atcaagtttg accccatggg gaaatacttt gccacaggaa gtgcagatgc cttggtcagc
      781 ctttgggacg ttgacgagtt agtgtgcgtg cggtgctttt ccagactaga ttggcctgtg
      841 aggaccctca gttttagcca cgatgggaaa atgctggcat cggcttcgga ggaccatttc
      901 attgatatag ctgaagtcga gacaggagac aagctatggg aggtgcagtg tgagtccccg
      961 actttcactg tggcttggca ccccaagagg cctctgctgg cttttgcctg tgatgacaaa
     1021 gatggcaaat acgacagcag tcgggaggct ggaactgtga agctgtttgg acttcccaat
     1081 gactcctgag agggccgaca gcgagaggag agaggaggca ggaggccggg cggactattt
     1141 gggctaccta atgactgtac ctttcaggga gaggaggcct gggcttccta atgactcctg
     1201 agaggacctg atgggagagg aggctgggtg cagaccttcc ttttgtatag ttcaatgtag
     1261 tcttttggac ttgtaggcac cataaatgtt tataaatgtc ataaatgacc aaaaaaaaaa
     1321 aaaaaaacca aaccaaaaaa ccaaaaccaa aacggcccaa aaatctttgg tcaggctgag
     1381 tgtaccttaa ccctctctgt gatactggag aactgagcta gctttactgt ggtgagcaag
     1441 tgccccacat tagctccagc cccagcccca gccccacctc ctgggttttg tgttggtgta
     1501 tttgtgttag cagttcccat ccctccccgc tgctgacagc tagctaatga ggattttgca
     1561 agtgcaggtc atggtctgtt ttgttttagg gttctctaga ggaacaaaac tggtagacta
     1621 tctgcctaag aacagaactg atgtggaaca tctgtttgtc tgtgtatata tctgtgtctc
     1681 cgtatgggat tttatcagag tggcttagag gctgtggtct ggatagtcag tccagctgtc
     1741 tcctgatggg aaggccaagg atccagtagt tgttcagttg ttcagactgg atgtctcagc
     1801 agtgttactc tggtgctgga gtcccgaggg atccctggag agctgctgat cgtggtctgt
     1861 gttggaatcc tgaagaagta ggttctgtgg ttagcagcag aggaggcccc ggcagcaggg
     1921 tagatgaacc aggcggtgag agtgagggca agcaggggaa aagccatagc ttgctttttc
     1981 cctgtccttt tatataggct gccaccagcg tgtgtgtccc agatttaggg tgggtcttcc
     2041 cacctcaaag gatcaaggaa aatctctcac aggtgtgccc tgctggttgg gtttcagtgt
     2101 ttccagatgc cacagtcaaa gttgacagcc aagatagccc atcacttgag tagcatagca
     2161 tgccctctcc tgctgggagg gccctgtttt ctggatttga ccatggcaga gtttgttgga
     2221 catctgctta ggcggtactg gtttaaaggg agggtagcag catagggatg ggatttttga
     2281 aaagcaattt tgagaacaga gcattcaata aaactggctt tcatta
//
