LOCUS       NM_001417745            3936 bp    mRNA    linear   ROD 19-AUG-2024
DEFINITION  Mus musculus THO complex 1 (Thoc1), transcript variant 2, mRNA.
ACCESSION   NM_001417745
VERSION     NM_001417745.1
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3936)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 3936)
  AUTHORS   Bhattacharjee,R., Jolly,L.A., Corbett,M.A., Wee,I.C., Rao,S.R.,
            Gardner,A.E., Ritchie,T., van Hugte,E.J.H., Ciptasari,U., Piltz,S.,
            Noll,J.E., Nazri,N., van Eyk,C.L., White,M., Fornarino,D.,
            Poulton,C., Baynam,G., Collins-Praino,L.E., Snel,M.F., Nadif
            Kasri,N., Hemsley,K.M., Thomas,P.Q., Kumar,R. and Gecz,J.
  TITLE     Compromised transcription-mRNA export factor THOC2 causes R-loop
            accumulation, DNA damage and adverse neurodevelopment
  JOURNAL   Nat Commun 15 (1), 1210 (2024)
   PUBMED   38331934
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3936)
  AUTHORS   Kafer,G.R. and Cesare,A.J.
  TITLE     A Survey of Essential Genome Stability Genes Reveals That
            Replication Stress Mitigation Is Critical for Peri-Implantation
            Embryogenesis
  JOURNAL   Front Cell Dev Biol 8, 416 (2020)
   PUBMED   32548123
  REMARK    Review article
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 3936)
  AUTHORS   Chinnam,M., Wang,X., Zhang,X. and Goodrich,D.W.
  TITLE     Evaluating Effects of Hypomorphic Thoc1 Alleles on Embryonic
            Development in Rb1 Null Mice
  JOURNAL   Mol Cell Biol 36 (11), 1621-1627 (2016)
   PUBMED   27001308
  REMARK    GeneRIF: Results found that Thoc1 deficiency delays embryo death,
            and suggest that Thoc1 is required to support increased expression
            of E2f and apoptotic regulatory genes that trigger apoptosis upon
            Rb1 loss.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 3936)
  AUTHORS   Chinnam,M., Wang,Y., Zhang,X., Gold,D.L., Khoury,T., Nikitin,A.Y.,
            Foster,B.A., Li,Y., Bshara,W., Morrison,C.D., Payne Ondracek,R.D.,
            Mohler,J.L. and Goodrich,D.W.
  TITLE     The Thoc1 ribonucleoprotein and prostate cancer progression
  JOURNAL   J Natl Cancer Inst 106 (11) (2014)
   PUBMED   25296641
  REMARK    GeneRIF: Thoc1 gene deletion prevents prostate cancer progression
            in mice, but has little effect on normal tissue. Prostate cancer
            cells deprived of Thoc1 show gene expression defects that
            compromise cell growth.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 3936)
  AUTHORS   Wang,X., Chinnam,M., Wang,J., Wang,Y., Zhang,X., Marcon,E.,
            Moens,P. and Goodrich,D.W.
  TITLE     Thoc1 deficiency compromises gene expression necessary for normal
            testis development in the mouse
  JOURNAL   Mol Cell Biol 29 (10), 2794-2803 (2009)
   PUBMED   19307311
  REMARK    GeneRIF: These findings support the notion that Thoc1-mediated RNP
            assembly contributes to the coordinated expression of genes
            necessary for normal differentiation and development in vivo.
REFERENCE   7  (bases 1 to 3936)
  AUTHORS   Wang,X., Li,Y., Zhang,X. and Goodrich,D.W.
  TITLE     An allelic series for studying the mouse Thoc1 gene
  JOURNAL   Genesis 45 (1), 32-37 (2007)
   PUBMED   17211872
REFERENCE   8  (bases 1 to 3936)
  AUTHORS   Wang,X., Chang,Y., Li,Y., Zhang,X. and Goodrich,D.W.
  TITLE     Thoc1/Hpr1/p84 is essential for early embryonic development in the
            mouse
  JOURNAL   Mol Cell Biol 26 (11), 4362-4367 (2006)
   PUBMED   16705185
  REMARK    GeneRIF: The generation and phenotypic characterization of mice
            containing a null allele of the Thoc1 gene is described.
REFERENCE   9  (bases 1 to 3936)
  AUTHORS   Reed,J.C., Doctor,K., Rojas,A., Zapata,J.M., Stehlik,C.,
            Fiorentino,L., Damiano,J., Roth,W., Matsuzawa,S., Newman,R.,
            Takayama,S., Marusawa,H., Xu,F., Salvesen,G. and Godzik,A.
  CONSRTM   RIKEN GER Group; GSL Members
  TITLE     Comparative analysis of apoptosis and inflammation genes of mice
            and humans
  JOURNAL   Genome Res 13 (6B), 1376-1388 (2003)
   PUBMED   12819136
REFERENCE   10 (bases 1 to 3936)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC114677.13 and AC163451.2.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR13422603.413250.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849384, SAMN01164133
                                           [ECO:0000348]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-71                AC114677.13        30312-30382         c
            72-145              AC114677.13        29387-29460         c
            146-206             AC114677.13        29243-29303         c
            207-273             AC114677.13        28006-28072         c
            274-467             AC114677.13        26067-26260         c
            468-516             AC114677.13        25247-25295         c
            517-612             AC114677.13        24640-24735         c
            613-695             AC114677.13        19725-19807         c
            696-769             AC114677.13        18230-18303         c
            770-878             AC114677.13        12851-12959         c
            879-1010            AC114677.13        10580-10711         c
            1011-1111           AC114677.13        4024-4124           c
            1112-1178           AC114677.13        2203-2269           c
            1179-1229           AC114677.13        1955-2005           c
            1230-1300           AC114677.13        1797-1867           c
            1301-1396           AC114677.13        1393-1488           c
            1397-1462           AC114677.13        894-959             c
            1463-1546           AC163451.2         59263-59346         c
            1547-1694           AC163451.2         56762-56909         c
            1695-1770           AC163451.2         56130-56205         c
            1771-3936           AC163451.2         53551-55716         c
FEATURES             Location/Qualifiers
     source          1..3936
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="18"
                     /map="18 4.91 cM"
     gene            1..3936
                     /gene="Thoc1"
                     /gene_synonym="3110002N20Rik; NMP-84; tho1"
                     /note="THO complex 1"
                     /db_xref="GeneID:225160"
                     /db_xref="MGI:MGI:1919668"
     exon            1..71
                     /gene="Thoc1"
                     /gene_synonym="3110002N20Rik; NMP-84; tho1"
                     /inference="alignment:Splign:2.1.0"
     CDS             18..2066
                     /gene="Thoc1"
                     /gene_synonym="3110002N20Rik; NMP-84; tho1"
                     /note="isoform 2 is encoded by transcript variant 2; THO
                     complex subunit 1; nuclear matrix protein p84"
                     /codon_start=1
                     /product="THO complex subunit 1 isoform 2"
                     /protein_id="NP_001404674.1"
                     /db_xref="GeneID:225160"
                     /db_xref="MGI:MGI:1919668"
                     /translation="MSPTPALFSLPEARTRFTKSTREALNNKNIKPLLTAFSQLPGSE
                     NEKKCTLDQAFRGVLEEEIINHSACENVLAIISLAIGGVTETCPWILFLVLEGSVTSS
                     SLIYTPFVGVCTASTPFVLLGDVLDCLPLDQCDTIFTFVEKNVATWKSNTFYSAGKNY
                     LLRMCNDLLRRLSKSQNTVFCGRIQLFLARLFPLSEKSGLNLQSQFNLENVTVFNTNE
                     QESTLGQKHTEDREEGMDVEEGEMGDDEAPTTCSIPIDYNLYRKFWSLQDYFRNPVQC
                     YEKISWKTFLKYSEEVLAVFKSYKLDDTQASRKKMEELKTGGEHVYFAKFLTSEKLMD
                     LQLSDSNFRRHILLQYLILFQYLKGQVKFKSSNYVLTDEQSLWIEDTTKSVYQLLSEN
                     PPDGERFSKMVEHILNTEENWNSWKNEGCPSFVKERASDTKPTRVVRKRAAPEDFLGK
                     GPNKKILIGNEELTRLWNLCPDNMEACKSETREYMPTLEEFFEEAIEQADPENMVESE
                     YKAVNNSNYGWRALRLLARRSPHFFQPTNQQFKSLPEYLENMVIKLAKELPPPSEEIK
                     TGEDEDEEDNDALLKENESPDVRRDKPITGEQIESFANKLGEQWKILAPYLEIKDSDI
                     RQIECDSEDMKMRAKQLLVAWQDQEGVHATTDNLISALNKSGLSDLAESLTNDTETNS
                     "
     exon            72..145
                     /gene="Thoc1"
                     /gene_synonym="3110002N20Rik; NMP-84; tho1"
                     /inference="alignment:Splign:2.1.0"
     exon            146..206
                     /gene="Thoc1"
                     /gene_synonym="3110002N20Rik; NMP-84; tho1"
                     /inference="alignment:Splign:2.1.0"
     exon            207..273
                     /gene="Thoc1"
                     /gene_synonym="3110002N20Rik; NMP-84; tho1"
                     /inference="alignment:Splign:2.1.0"
     exon            274..467
                     /gene="Thoc1"
                     /gene_synonym="3110002N20Rik; NMP-84; tho1"
                     /inference="alignment:Splign:2.1.0"
     exon            468..516
                     /gene="Thoc1"
                     /gene_synonym="3110002N20Rik; NMP-84; tho1"
                     /inference="alignment:Splign:2.1.0"
     exon            517..612
                     /gene="Thoc1"
                     /gene_synonym="3110002N20Rik; NMP-84; tho1"
                     /inference="alignment:Splign:2.1.0"
     exon            613..695
                     /gene="Thoc1"
                     /gene_synonym="3110002N20Rik; NMP-84; tho1"
                     /inference="alignment:Splign:2.1.0"
     exon            696..769
                     /gene="Thoc1"
                     /gene_synonym="3110002N20Rik; NMP-84; tho1"
                     /inference="alignment:Splign:2.1.0"
     exon            770..878
                     /gene="Thoc1"
                     /gene_synonym="3110002N20Rik; NMP-84; tho1"
                     /inference="alignment:Splign:2.1.0"
     exon            879..1010
                     /gene="Thoc1"
                     /gene_synonym="3110002N20Rik; NMP-84; tho1"
                     /inference="alignment:Splign:2.1.0"
     exon            1011..1111
                     /gene="Thoc1"
                     /gene_synonym="3110002N20Rik; NMP-84; tho1"
                     /inference="alignment:Splign:2.1.0"
     exon            1112..1178
                     /gene="Thoc1"
                     /gene_synonym="3110002N20Rik; NMP-84; tho1"
                     /inference="alignment:Splign:2.1.0"
     exon            1179..1229
                     /gene="Thoc1"
                     /gene_synonym="3110002N20Rik; NMP-84; tho1"
                     /inference="alignment:Splign:2.1.0"
     exon            1230..1300
                     /gene="Thoc1"
                     /gene_synonym="3110002N20Rik; NMP-84; tho1"
                     /inference="alignment:Splign:2.1.0"
     exon            1301..1396
                     /gene="Thoc1"
                     /gene_synonym="3110002N20Rik; NMP-84; tho1"
                     /inference="alignment:Splign:2.1.0"
     exon            1397..1462
                     /gene="Thoc1"
                     /gene_synonym="3110002N20Rik; NMP-84; tho1"
                     /inference="alignment:Splign:2.1.0"
     exon            1463..1546
                     /gene="Thoc1"
                     /gene_synonym="3110002N20Rik; NMP-84; tho1"
                     /inference="alignment:Splign:2.1.0"
     exon            1547..1694
                     /gene="Thoc1"
                     /gene_synonym="3110002N20Rik; NMP-84; tho1"
                     /inference="alignment:Splign:2.1.0"
     exon            1695..1770
                     /gene="Thoc1"
                     /gene_synonym="3110002N20Rik; NMP-84; tho1"
                     /inference="alignment:Splign:2.1.0"
     exon            1771..3936
                     /gene="Thoc1"
                     /gene_synonym="3110002N20Rik; NMP-84; tho1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2157..2162
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Thoc1"
                     /gene_synonym="3110002N20Rik; NMP-84; tho1"
                     /note="hexamer: AATAAA"
     polyA_site      2175
                     /gene="Thoc1"
                     /gene_synonym="3110002N20Rik; NMP-84; tho1"
                     /note="major polyA site"
     regulatory      3055..3060
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Thoc1"
                     /gene_synonym="3110002N20Rik; NMP-84; tho1"
                     /note="hexamer: AATAAA"
     polyA_site      3080
                     /gene="Thoc1"
                     /gene_synonym="3110002N20Rik; NMP-84; tho1"
     regulatory      3911..3916
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Thoc1"
                     /gene_synonym="3110002N20Rik; NMP-84; tho1"
                     /note="hexamer: ATTAAA"
     polyA_site      3936
                     /gene="Thoc1"
                     /gene_synonym="3110002N20Rik; NMP-84; tho1"
ORIGIN      
        1 gggcacgcgc aaccaagatg tctccgacgc cggcgctttt cagtttgccg gaggcgcgga
       61 cgcgatttac gaaatctacc agagaagcct tgaataacaa aaatatcaag ccgttgttga
      121 ctgccttcag tcagttacct ggcagtgaaa atgaaaaaaa atgtaccctt gaccaagctt
      181 tcagaggtgt tctggaagaa gaaattataa atcattccgc atgtgagaac gttttagcta
      241 ttatttctct tgctattggg ggagtgaccg aaacctgtcc atggattctc tttcttgtgc
      301 tggaaggcag tgtcactagc tcttcattga tttatacccc atttgtaggt gtttgtactg
      361 cgtccacacc atttgtgcta ttgggagatg tcttagactg ccttcctttg gatcagtgtg
      421 acacaatatt cacttttgtt gaaaaaaatg ttgctacttg gaagtcaaat acattctatt
      481 ctgctggcaa aaattattta ctgcgtatgt gcaatgatct cctcagaaga ttgtctaaat
      541 ctcagaatac agttttctgt ggaagaattc aactcttttt ggccaggctt tttcctctat
      601 ctgagaaatc aggtcttaat ttgcagagtc agtttaacct ggaaaacgtc actgttttca
      661 atacaaacga gcaggagagc accctgggtc agaagcatac tgaggataga gaggaaggga
      721 tggatgtaga agaaggtgaa atgggagatg atgaagcccc gacaacttgt tctattccaa
      781 ttgattacaa cctgtataga aaattctggt cacttcagga ttacttcagg aacccagtgc
      841 aatgctatga gaagatttct tggaaaacgt ttctcaagta ttctgaagaa gttttagctg
      901 tttttaaaag ttataaatta gatgatactc aagcctccag gaaaaagatg gaagaattga
      961 aaacaggagg agagcatgta tacttcgcca agtttctaac aagtgaaaag ttgatggatt
     1021 tacaactgag tgacagtaac tttcgtcgac acatcctgtt gcagtatctc attttatttc
     1081 aatatctcaa gggacaggtc aaattcaaaa gttcaaatta tgttttaact gatgagcaat
     1141 cactctggat tgaagatact acaaaatcag tctaccaact gctatctgaa aatccccctg
     1201 atggagaaag attttcaaag atggtagagc atatattaaa cactgaggaa aattggaact
     1261 catggaagaa tgaaggttgc ccaagttttg ttaaagaaag agcgtcagat accaagccca
     1321 caagagtggt gaggaagaga gcagccccag aagacttctt aggcaaaggg cccaacaaga
     1381 aaatcctcat tggaaatgag gagctcacaa ggctttggaa tctctgtcct gataatatgg
     1441 aagcctgtaa atcagagaca agagaataca tgccaacttt ggaggagttc tttgaagaag
     1501 ctattgaaca agcagatcct gaaaacatgg ttgaaagtga atataaagcc gtaaataatt
     1561 caaattatgg atggagagct cttagactct tagcccggag aagccctcac ttctttcagc
     1621 caaccaacca gcagtttaag agtctaccag agtatctgga aaacatggtg ataaagctgg
     1681 ccaaggaact accaccccct tctgaagaaa taaagacagg tgaggatgaa gacgaggaag
     1741 ataatgatgc tttactaaag gaaaatgaaa gtccagatgt tcggcgagat aaacctataa
     1801 caggggagca gatagagtca tttgccaaca aacttggtga gcagtggaag attttggctc
     1861 cttacctgga aattaaagac tctgatatta ggcagattga gtgtgacagt gaagacatga
     1921 agatgagagc caagcagctc cttgttgcct ggcaagatca agaaggagtt catgcaacaa
     1981 ctgataacct gattagtgca ctaaacaagt ctggattaag tgaccttgca gaaagtctaa
     2041 ctaatgacac tgaaacaaac agttagcttt cttcctttct tttctttttt attaaacagt
     2101 gataagattt tgttaccaag cagcatttga taaaaggttc actggttttg gtaaacaata
     2161 aacactttta taacagctat tgtacagttg gctggtgctc caaaaaccac atacagaaca
     2221 ggcattggag tggagaacag ggtgggaaaa caaagtaaaa ggacctcaag atcacaccaa
     2281 tttttgttaa gtatttctac agtgaagtta aatgagtact actaacagta ctgtcccaaa
     2341 ttagtttcct gcatgctatc ggttataaaa ttttattctg aaattacaag atgacacttc
     2401 tgaaacctta ggactggaga gaagaggatc agcaaaccat ggatagtttt aagtgttttc
     2461 ctggcactct gacatgctga cactacaata gcaaagagtt caaactgata gccaggggcc
     2521 tgcaaaaacc aaatcattag ctgtttgtcc aaaacagaaa atgtttgttt gctagctcta
     2581 agtttatatg aagtggagtc taagacccaa ctactccttt agctttacaa agattacttg
     2641 acctccttaa gtctttaaac acccagggat tcctgtcagt tcacttgaga tgtgaaaata
     2701 ccctatatgt agcagagcat gtgtggtact ggctgacggt taagatgttc gtgttctagt
     2761 cccatacagc atttcctgtg ggtgtttagt atactagtga agggcgactg ggcagaccac
     2821 tgttaagtgt tcttgcttga actgcactct actagctggg gaagcagtga ctaagcacaa
     2881 ccctggcctc cagatgtctt ctgcattaag caaattaagg caagttcagg gattgaaagg
     2941 gaggagaatg tttttttacc tcattctaaa acacttaaca ccttatttaa aacaaaatga
     3001 tggaagtcca atttctactt taaagaatta tttttggaat gcctttttag gtcaaataaa
     3061 ctatcaccac actttttgta taatagtttt cttaatgcat tctaaacata gttttggcat
     3121 atttgttcta aatctacctc acctggttta gtaggaaaac acaggtgcca aattccttcg
     3181 ttctacaact gttctagcaa ataacactga atcccagggg tcatgacaca aactattaca
     3241 gtacacacag cactgtttca ttccatgttt cactccccat tgtaaggcgt cttaactgct
     3301 aataatgttt aagttagcaa actatttaca aacctaaaat gctgacttac ccacactatt
     3361 acagatttat tatcctatag gaatcttggg tactttacag agcattaaaa catgaggtgg
     3421 aagatgtcat gatattcctg acattcctag tcaactcaaa tcagaccaga tcacgaatat
     3481 tcaacacttc atcctttcct tagattgcta aagttcctga aacatctaag gacttactca
     3541 ctgggtagtc aatagattaa taaacaatac tcttggtaat gtatacctta atcttaaaaa
     3601 tcagtaactt tcaagatggg cacaaagact ttgggaaaga aaatagaaac aacaaacgtg
     3661 tgagggaatg aaactgatca cagaattcac tgtccaaagg aactcatcac ctttactact
     3721 ttgtatttct tttgtaataa cactggacaa gctaaagttt cttgtatttt aaatagatcc
     3781 ttgactgctt agcattatta gccatttgca gaaactgtat agtactgaat tcatttgact
     3841 cttaagccaa gctctaatac taaaaagaaa tgtcaaaagt tgttaatccc tctttttcct
     3901 tatttttatt attaaatatc cttggtttcc aagcaa
//
