LOCUS       NM_028998               2551 bp    mRNA    linear   ROD 09-JUN-2024
DEFINITION  Mus musculus thymocyte selection associated family member 3
            (Themis3), mRNA.
ACCESSION   NM_028998 XM_484657 XM_977317
VERSION     NM_028998.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2551)
  AUTHORS   Fu,G., Vallee,S., Rybakin,V., McGuire,M.V., Ampudia,J.,
            Brockmeyer,C., Salek,M., Fallen,P.R., Hoerter,J.A., Munshi,A.,
            Huang,Y.H., Hu,J., Fox,H.S., Sauer,K., Acuto,O. and Gascoigne,N.R.
  TITLE     Themis controls thymocyte selection through regulation of T cell
            antigen receptor-mediated signaling
  JOURNAL   Nat Immunol 10 (8), 848-856 (2009)
   PUBMED   19597499
REFERENCE   2  (bases 1 to 2551)
  AUTHORS   Johnson,A.L., Aravind,L., Shulzhenko,N., Morgun,A., Choi,S.Y.,
            Crockford,T.L., Lambe,T., Domaschenz,H., Kucharska,E.M., Zheng,L.,
            Vinuesa,C.G., Lenardo,M.J., Goodnow,C.C., Cornall,R.J. and
            Schwartz,R.H.
  TITLE     Themis is a member of a new metazoan gene family and is required
            for the completion of thymocyte positive selection
  JOURNAL   Nat Immunol 10 (8), 831-839 (2009)
   PUBMED   19597497
  REMARK    Erratum:[Nat Immunol. 2010 Jan;11(1):97]
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC122445.3 and CT010496.14.
            
            On Jun 24, 2019 this sequence version replaced NM_028998.1.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK018663.1, SRR5189685.166398.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849377, SAMN00849378
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-201               AC122445.3         66956-67156
            202-336             AC122445.3         68320-68454
            337-810             CT010496.14        205995-206468       c
            811-2551            CT010496.14        200972-202712       c
FEATURES             Location/Qualifiers
     source          1..2551
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="17"
                     /map="17 37.88 cM"
     gene            1..2551
                     /gene="Themis3"
                     /gene_synonym="9130404H23Rik"
                     /note="thymocyte selection associated family member 3"
                     /db_xref="GeneID:74556"
                     /db_xref="MGI:MGI:1921806"
     exon            1..201
                     /gene="Themis3"
                     /gene_synonym="9130404H23Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             114..1823
                     /gene="Themis3"
                     /gene_synonym="9130404H23Rik"
                     /note="thymocyte-expressed molecule involved in selection
                     protein 3"
                     /codon_start=1
                     /product="protein THEMIS3"
                     /protein_id="NP_083274.1"
                     /db_xref="CCDS:CCDS50170.1"
                     /db_xref="GeneID:74556"
                     /db_xref="MGI:MGI:1921806"
                     /translation="MEQTWDSYISSLNQNSLPRQVEVTQGRYSGSLENLSFSKGDIIT
                     VVDLEPVFVRAELKDGDQVLDVVTIPLRYEGNFQLMADPVSFETVADLTRSVRLPQSP
                     VAPRSPPRFQNSIPISADNLPMKLRKGETLSLIGFQESRGRRLLQCKVLRKKPPLTVL
                     LPMDCRGHFLECQDDRFYSIDTIVRWKMLAGRKRTVRVQARHHPKLLGPLVPEHFRGH
                     LVLYPCFSVTANLLGETRVSIPSDLDISVTEIARLDRKPRTTMRQIYSMEESKFPVRV
                     KIMNVVQSESKEYPKPLKRGQLLTILKTEEVKKFVASEISQGKKGKCFLVPYTYQGLV
                     LRRGRYFYAVSDVAAAMKHGELCFQASQDYTSYLGSFASFRANECFLALKKSVVSAEI
                     HGELHRVEVLKCLNIATKAHVKLPLFAVGKFLELFDGARPGTLQELCQVTRLPCHVRV
                     TSPDPSMTVDPLYGTKELRIENVIIEQCLIAKDEPTLEDIISSADMYREWPETTFEIP
                     IEKISCEVLVVEERSWIADVRKERCRPLQSIQEVTKESLAFSNCLVIRRPPPPVPKPR
                     SLF"
     exon            202..336
                     /gene="Themis3"
                     /gene_synonym="9130404H23Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            337..810
                     /gene="Themis3"
                     /gene_synonym="9130404H23Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            811..2551
                     /gene="Themis3"
                     /gene_synonym="9130404H23Rik"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2536..2541
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Themis3"
                     /gene_synonym="9130404H23Rik"
                     /note="hexamer: AATAAA"
     polyA_site      2551
                     /gene="Themis3"
                     /gene_synonym="9130404H23Rik"
                     /note="major polyA site"
ORIGIN      
        1 agagtcatga tcaggtggct cattacctgg tccagagaga cctcttttgc cgtccgtcca
       61 ctgctctgag cacggctgac catgtcctgc aggataaaaa caattccagt gccatggagc
      121 aaacttggga ctcttacatc agttccttaa atcagaactc tttgcccaga caagtggagg
      181 tgacccaggg aagatattca gggtcacttg aaaacctgtc attctccaaa ggggatatca
      241 tcacggtggt ggacctggag ccagttttcg tcagagcaga actgaaggac ggtgatcaag
      301 ttctggacgt ggtcactata cctcttagat atgaaggaaa cttccaactg atggcagacc
      361 cggtttcttt tgaaactgtg gctgatttaa cccgttctgt ccgacttccc cagagtcctg
      421 tggcccctag aagtcctccc cgcttccaga actcaatccc catctccgca gacaatttgc
      481 ccatgaagct caggaaagga gaaacactgt ccttaattgg ctttcaagag agtcggggga
      541 ggaggctgct gcaatgcaag gtgctgagga agaagccacc gctgacagtg ctcctcccca
      601 tggactgcag gggccacttc ctggagtgcc aggacgacag gttctactcc attgacacca
      661 tcgtgaggtg gaagatgctt gcagggagga aacgcacggt tagagtgcaa gcaagacatc
      721 acccgaagct ccttggcccc ctggttccag agcactttcg tggacattta gttttgtatc
      781 cctgtttctc agtgacggca aatctgctag gtgagacccg ggttagcatc ccctccgatc
      841 tggatatcag tgttactgag attgcccgcc tcgatcgtaa gcccaggaca acgatgagac
      901 aaatatacag catggaagaa agcaagttcc cagtgagagt taaaataatg aatgtggtcc
      961 agtcggagag caaggagtac ccaaagcctc tgaaacgtgg gcagctctta accatcctca
     1021 agactgaaga ggtgaaaaaa ttcgttgcct ctgagatttc ccaaggaaag aaagggaagt
     1081 gttttctcgt cccatacacc tatcagggcc tggtgcttag gagaggtcgt tatttttatg
     1141 ctgtctctga tgttgctgct gccatgaagc atggagagct ctgcttccag gcctcccagg
     1201 actacacctc ctacctggga tcgtttgcat ctttcagagc caacgagtgc ttcctggccc
     1261 tgaagaagag tgtggtttcc gccgagattc acggtgagct gcacagggtg gaagtgctca
     1321 agtgcctgaa cattgccact aaagcccacg tcaaactgcc cttgtttgcc gtaggtaagt
     1381 tcctggagct gtttgacggc gctaggccag ggaccctgca ggagctatgc caagtcacaa
     1441 gactgccgtg ccacgtcaga gtgaccagcc cagatccctc catgactgta gaccctctat
     1501 atggaactaa ggaactgaga attgaaaatg tcatcattga acaatgcctc atagccaaag
     1561 atgaaccaac actggaggac ataatctcct cagcagacat gtacagggag tggccagaaa
     1621 ccacatttga aatcccgatt gagaagataa gctgtgaggt gttagtggta gaagagcgtt
     1681 cgtggatagc tgatgtcagg aaagagagat gcaggcctct ccagagcatc caggaggtca
     1741 ctaaggagtc tctggccttt agtaattgct tggttattcg ccgtccacca ccacctgttc
     1801 caaagcccag aagtttgttc taaaaaaaaa gaaaatatga ctactatccc ttgctttgta
     1861 tacaggatca ctagcacgct caggaactga gtatctggag ttctctagag ccgaaaagga
     1921 gcccccagag gtgggagccc agaggaaatc gcaggtgttg agacgtgatt tccatcaccc
     1981 ctgtatgttt gctatacgtt gtgttatcat tctgttactg tgacataatg cttggataat
     2041 cagctcatgg gggtggggga gtggtgcgga agggaaggtt cctttgggct cagagtttca
     2101 gagatttgag ttcatgacca cctggcccca ctgccatcac agtgggaaca tgcagtggat
     2161 caaagctgcc cacctcatga ttggtgggtg ggaagcggaa aggttcccat gtccctttct
     2221 ataagccccc aggaacataa ctcccttcat caaggcgcca cctatttatt agtggctccc
     2281 aaaagcatag tctccattat ctcccaaaag tactgcatgc tggagaccaa gcctttaaca
     2341 cacgagcctt tgtgggactc ctattaaaag cattgcggct gggctggaga gatggctggg
     2401 attgctcttc cagaggtcct gagttcaatt cccagcaaca aaatggtggc tcacaaccat
     2461 ctgtaatggg ctccgacgcc ctcttcttgt gtgtgtgtct gaatctacag tgtactcata
     2521 cgcataaaat aaataaataa agaccatccc a
//
