LOCUS       NM_001033308            2542 bp    mRNA    linear   ROD 04-JUN-2024
DEFINITION  Mus musculus thymocyte selection associated family member 2
            (Themis2), mRNA.
ACCESSION   NM_001033308 XM_131779 XM_900087 XM_915499 XM_923772
VERSION     NM_001033308.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2542)
  AUTHORS   Deborah,E.A., Nabekura,T., Shibuya,K. and Shibuya,A.
  TITLE     THEMIS2 Impairs Antitumor Activity of NK Cells by Suppressing
            Activating NK Receptor Signaling
  JOURNAL   J Immunol 212 (11), 1819-1828 (2024)
   PUBMED   38619282
  REMARK    GeneRIF: THEMIS2 Impairs Antitumor Activity of NK Cells by
            Suppressing Activating NK Receptor Signaling.
REFERENCE   2  (bases 1 to 2542)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   3  (bases 1 to 2542)
  AUTHORS   Nabekura,T., Deborah,E.A., Tahara,S., Arai,Y., Love,P.E., Kako,K.,
            Fukamizu,A., Muratani,M. and Shibuya,A.
  TITLE     Themis2 regulates natural killer cell memory function and formation
  JOURNAL   Nat Commun 14 (1), 7200 (2023)
   PUBMED   37938555
  REMARK    GeneRIF: Themis2 regulates natural killer cell memory function and
            formation.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2542)
  AUTHORS   Ingham,N.J., Pearson,S.A., Vancollie,V.E., Rook,V., Lewis,M.A.,
            Chen,J., Buniello,A., Martelletti,E., Preite,L., Lam,C.C.,
            Weiss,F.D., Powis,Z., Suwannarat,P., Lelliott,C.J., Dawson,S.J.,
            White,J.K. and Steel,K.P.
  TITLE     Mouse screen reveals multiple new genes underlying mouse and human
            hearing loss
  JOURNAL   PLoS Biol 17 (4), e3000194 (2019)
   PUBMED   30973865
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2542)
  AUTHORS   Cheng,D., Deobagkar-Lele,M., Zvezdova,E., Choi,S., Uehara,S.,
            Baup,D., Bennett,S.C., Bull,K.R., Crockford,T.L., Ferry,H.,
            Warzecha,C., Marcellin,M., de Peredo,A.G., Lesourne,R.,
            Anzilotti,C., Love,P.E. and Cornall,R.J.
  TITLE     Themis2 lowers the threshold for B cell activation during positive
            selection
  JOURNAL   Nat Immunol 18 (2), 205-213 (2017)
   PUBMED   27992403
  REMARK    GeneRIF: this stuidy identifies a unique function for Themis2 in
            differential signaling and provide insight into how B cells
            discriminate between antigens of different quantity and quality
REFERENCE   6  (bases 1 to 2542)
  AUTHORS   Hartweger,H., Schweighoffer,E., Davidson,S., Peirce,M.J., Wack,A.
            and Tybulewicz,V.L.
  TITLE     Themis2 is not required for B cell development, activation, and
            antibody responses
  JOURNAL   J Immunol 193 (2), 700-707 (2014)
   PUBMED   24907343
  REMARK    GeneRIF: Data indicate that Themis2 protein is not required for B
            cell development, for activation, or for antibody responses either
            to model Ags or to influenza viral infection.
REFERENCE   7  (bases 1 to 2542)
  AUTHORS   Lesourne,R., Zvezdova,E., Song,K.D., El-Khoury,D., Uehara,S.,
            Barr,V.A., Samelson,L.E. and Love,P.E.
  TITLE     Interchangeability of Themis1 and Themis2 in thymocyte development
            reveals two related proteins with conserved molecular function
  JOURNAL   J Immunol 189 (3), 1154-1161 (2012)
   PUBMED   22732588
REFERENCE   8  (bases 1 to 2542)
  AUTHORS   Peirce,M.J., Brook,M., Morrice,N., Snelgrove,R., Begum,S.,
            Lanfrancotti,A., Notley,C., Hussell,T., Cope,A.P. and Wait,R.
  TITLE     Themis2/ICB1 is a signaling scaffold that selectively regulates
            macrophage Toll-like receptor signaling and cytokine production
  JOURNAL   PLoS One 5 (7), e11465 (2010)
   PUBMED   20644716
  REMARK    GeneRIF: Study hypothesise that Themis2 may constitute a novel,
            physiological control point in macrophage inflammatory responses.
            Publication Status: Online-Only
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from AK150471.1.
            
            On Apr 6, 2007 this sequence version replaced NM_001033308.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK150471.1, AK153536.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-2542              AK150471.1         2-2543
FEATURES             Location/Qualifiers
     source          1..2542
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="4"
                     /map="4 65.9 cM"
     gene            1..2542
                     /gene="Themis2"
                     /gene_synonym="ICB-1"
                     /note="thymocyte selection associated family member 2"
                     /db_xref="GeneID:230787"
                     /db_xref="MGI:MGI:2446213"
     exon            1..105
                     /gene="Themis2"
                     /gene_synonym="ICB-1"
                     /inference="alignment:Splign:2.1.0"
     CDS             12..2003
                     /gene="Themis2"
                     /gene_synonym="ICB-1"
                     /note="basement membrane-induced protein; protein ICB-1;
                     induced by contact to basement membrane 1 protein;
                     thymocyte-expressed molecule involved in selection protein
                     2"
                     /codon_start=1
                     /product="protein THEMIS2"
                     /protein_id="NP_001028480.1"
                     /db_xref="CCDS:CCDS18735.1"
                     /db_xref="GeneID:230787"
                     /db_xref="MGI:MGI:2446213"
                     /translation="MEPVPLQDFVSGLDPTSLPRVLRVCSGVYFEGSVYELFGNECCL
                     STGDLIKVTHVQLQKVVCEYPETGQTLELNPNFTGLFSPLTSLRSYRTLEDLVSAMPQ
                     NSTRWPIYFKSTQRIVTKASVVPEDQPLRLEAVEIHHGIRYARCVQVSKTKELLHLPL
                     SQKGPFWRCKPSAPQTLHQILQDPALKDLTLSCPSLPWNSVILKPQYMLQAIMHMRSS
                     IVKIPSTLEVEVEDVTASSQHIHFFKPLRLSEVLAGGGPFPLTTEILEVPEGPPVFLS
                     PWVSFLRKGQRLCIYGPASPSWRVVASSKSRKVPRYFMLSGAYQGKLKRRPREFSTAY
                     DLLGALQPGRPLRVVATKDCDGNEEENPDFSFLAVGDRLEVLRSGQVCGTKGQDIDVL
                     VCQRLSEQSGEEEEDLEEIEDEAEDKEQILLPLYLSGSFVEEVNDSRRYNLVDLTAQY
                     SLPCEVKVVTKDTRHPTDPLASFPGLRLEEKLTEPFLVVSLDSQPEMCFEIPPRWLDL
                     TVVEAEGQPAQVARPLSIAPVEELSEAFYYSLRKLPASESQAPPPRPPKSQGINKKQQ
                     NIQSCKESSVKPQVVEPQKSCPQPQLKAKTLEALPKNKSNVYSKISVHKKDRKPNPQT
                     QNSVLSMKPKTSSSLGKHSTMESHLLPDPDMDDHDYEEI"
     exon            106..246
                     /gene="Themis2"
                     /gene_synonym="ICB-1"
                     /inference="alignment:Splign:2.1.0"
     exon            247..654
                     /gene="Themis2"
                     /gene_synonym="ICB-1"
                     /inference="alignment:Splign:2.1.0"
     exon            655..1742
                     /gene="Themis2"
                     /gene_synonym="ICB-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1743..1893
                     /gene="Themis2"
                     /gene_synonym="ICB-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1894..2542
                     /gene="Themis2"
                     /gene_synonym="ICB-1"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 ggaacgcgac catggagccg gtgccgctgc aggactttgt gagcggcctg gaccccacct
       61 cccttccgcg cgtgctgcgg gtctgctccg gagtctactt tgagggttcc gtctacgagc
      121 tctttgggaa tgagtgctgc ctctccaccg gggacctaat caaggtcact catgtccagc
      181 tccaaaaggt tgtgtgcgag tatccagaga cgggtcagac gttggagctc aaccccaatt
      241 tcacaggcct cttcagccca ctcaccagcc tgcgaagcta cagaaccctg gaggaccttg
      301 tctctgccat gccccagaac tccacccggt ggcccattta cttcaagtca actcagagaa
      361 ttgtcaccaa ggccagtgtg gtgcctgagg atcagcccct cagacttgag gctgtggaaa
      421 tacaccatgg gatccgctat gcgcgctgcg tccaggtctc aaagaccaag gagctccttc
      481 atctgcccct ttcccagaag ggacccttct ggagatgcaa gcccagtgct ccacaaactc
      541 tgcaccaaat cctgcaggac ccagctctga aggacctcac gctcagctgc cccagcctac
      601 cctggaactc cgtgatccta aaaccccagt atatgctcca agccatcatg cacatgcgca
      661 gcagcatcgt caagatcccg tctacgctgg aggtggaggt agaagacgtc actgcttcct
      721 cccaacacat ccacttcttc aaacccttgc ggctgagcga ggtgttggct gggggaggcc
      781 ccttccctct gaccacggag atcctggagg ttcctgaggg tccgcccgtc ttcctcagcc
      841 cgtgggtgag cttcctacgc aaaggccaga ggctctgcat ctatggtcca gcttcaccat
      901 cctggcgggt tgtggcctcc agcaagagcc gaaaagtgcc cagatacttc atgctctctg
      961 gggcctacca gggcaagctc aagcggaggc ccagggagtt ctccaccgcc tatgaccttt
     1021 tgggggctct ccagccaggc cggcccctcc gggtggtagc cacaaaagac tgtgatggga
     1081 atgaggaaga gaatcctgac ttttctttct tggctgtggg tgatcggctg gaagtgctga
     1141 ggtcaggcca ggtctgtggg accaaaggcc aggacataga tgtcctggta tgtcagcggc
     1201 tgagtgagca gtctggggag gaagaggagg acttggagga gatcgaggac gaggcagagg
     1261 acaaagagca aattctgcta cccctctacc tctctgggag ctttgtggag gaggtgaacg
     1321 acagccgacg ctataaccta gtggatctca cggcccagta ctccctgccc tgtgaggtca
     1381 aggtggtgac caaggatacc cgtcacccca ctgaccccct ggcttccttc cctggcctgc
     1441 ggctggagga gaagctcaca gaaccctttt tggtggtaag cctggactcc cagccagaaa
     1501 tgtgctttga gatccctccc cggtggctgg acctgactgt ggtggaggct gagggacagc
     1561 cagcccaggt ggctcggccg ctgtccatcg ctccggtgga ggaactgtca gaagctttct
     1621 actacagcct ccggaagctg ccagcctctg agagccaagc ccccccgccc aggcccccaa
     1681 agagccaggg tatcaataag aaacagcaga acatacagag ctgtaaagag agcagtgtaa
     1741 agcctcaagt tgtagaaccg cagaaatcct gcccgcagcc tcaactcaag gcgaagacct
     1801 tggaagccct tcccaagaac aaatcaaatg tgtacagcaa gatttctgtc cacaagaaag
     1861 accgcaaacc caacccccaa acacagaatt cagtgctgag tatgaagccc aaaacctcga
     1921 gctccctcgg caagcactct accatggagt cacatctcct gcctgacccg gatatggatg
     1981 accatgacta tgaagaaatt tgaacacgtt cagaaaaaag tcatctaaag tgtagggaag
     2041 ccacatttcc agttgccacc acacaggact cggatactag ctaactcttg gaggacctag
     2101 ggcaaagtct caggggactt agactccaat gggcatggga ccgatactat ccctgcctct
     2161 ttgggttcca ggtcggcttg ttgcatgtga caccctcagc cttagagcgt tggtgtccag
     2221 agaagccatt caggttctgg gccctcctat gcccatcagt tgtcccacta ctaggcacag
     2281 agaaggatca aaggccacca gcttcagcat cgccctgcct ctcagtaaag agaactcagt
     2341 tctgattgaa ctacccaatg taggccacct cctttgtttg ctctgcactt gtgcagtgca
     2401 ggctctgtgc ctaggggcag acagtattct cttgttggta gagcccagct catgatcaat
     2461 aatacccagt aaatgggctt gtgtgactga atgtagggat ctgcctccaa gaataaaatc
     2521 ctaggtagga cactagtact cc
//
