LOCUS       NM_178666               5085 bp    mRNA    linear   ROD 09-FEB-2025
DEFINITION  Mus musculus thymocyte selection associated (Themis), transcript
            variant 1, mRNA.
ACCESSION   NM_178666
VERSION     NM_178666.6
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 5085)
  AUTHORS   Risemberg,E.L., Smeekens,J.M., Cruz Cisneros,M.C., Hampton,B.K.,
            Hock,P., Linnertz,C.L., Miller,D.R., Orgel,K., Shaw,G.D., de
            Villena,F.P.M., Burks,A.W., Valdar,W., Kulis,M.D. and Ferris,M.T.
  TITLE     A mutation in Themis contributes to anaphylaxis severity following
            oral peanut challenge in CC027 mice
  JOURNAL   J Allergy Clin Immunol 154 (2), 387-397 (2024)
   PUBMED   38670234
REFERENCE   2  (bases 1 to 5085)
  AUTHORS   Melique,S., Vadel,A., Rouquie,N., Yang,C., Bories,C., Cotineau,C.,
            Saoudi,A., Fazilleau,N. and Lesourne,R.
  TITLE     THEMIS promotes T cell development and maintenance by rising the
            signaling threshold of the inhibitory receptor BTLA
  JOURNAL   Proc Natl Acad Sci U S A 121 (20), e2318773121 (2024)
   PUBMED   38713628
  REMARK    GeneRIF: THEMIS promotes T cell development and maintenance by
            rising the signaling threshold of the inhibitory receptor BTLA.
REFERENCE   3  (bases 1 to 5085)
  AUTHORS   Marrocco,R., Bernard,I., Joulia,E., Barascud,R., Dejean,A.S.,
            Lesourne,R. and Saoudi,A.
  TITLE     Positive regulation of Vav1 by Themis controls CD4 T cell
            pathogenicity in a mouse model of central nervous system
            inflammation
  JOURNAL   Cell Mol Life Sci 81 (1), 161 (2024)
   PUBMED   38565808
  REMARK    GeneRIF: Positive regulation of Vav1 by Themis controls CD4 T cell
            pathogenicity in a mouse model of central nervous system
            inflammation.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 5085)
  AUTHORS   Gautam,N., Wojciech,L., Yap,J., Chua,Y.L., Ding,E.M., Sim,D.C.,
            Tan,A.S., Ahl,P.J., Prasad,M., Tung,D.W., Connolly,J.E.,
            Adriani,G., Brzostek,J. and Gascoigne,N.R.
  TITLE     Themis controls T cell activation, effector functions, and
            metabolism of peripheral CD8+ T cells
  JOURNAL   Life Sci Alliance 6 (12), e202302156 (2023)
   PUBMED   37739454
  REMARK    GeneRIF: Themis controls T cell activation, effector functions, and
            metabolism of peripheral CD8[+] T cells.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 5085)
  AUTHORS   Choi,S., Hatzihristidis,T., Gaud,G., Dutta,A., Lee,J., Arya,A.,
            Clubb,L.M., Stamos,D.B., Markovics,A., Mikecz,K. and Love,P.
  TITLE     GRB2 promotes thymocyte positive selection by facilitating
            THEMIS-mediated inactivation of SHP1
  JOURNAL   J Exp Med 220 (7) (2023)
   PUBMED   37067793
REFERENCE   6  (bases 1 to 5085)
  AUTHORS   Patrick,M.S., Oda,H., Hayakawa,K., Sato,Y., Eshima,K., Kirikae,T.,
            Iemura,S., Shirai,M., Abe,T., Natsume,T., Sasazuki,T. and Suzuki,H.
  TITLE     Gasp, a Grb2-associating protein, is critical for positive
            selection of thymocytes
  JOURNAL   Proc Natl Acad Sci U S A 106 (38), 16345-16350 (2009)
   PUBMED   19805304
  REMARK    GeneRIF: Study described a gene called Gasp that is critical for
            positive selection.
            GeneRIF: Gasp associates with Grb2 and is required for positive
            selection of thymocytes
REFERENCE   7  (bases 1 to 5085)
  AUTHORS   Kakugawa,K., Yasuda,T., Miura,I., Kobayashi,A., Fukiage,H.,
            Satoh,R., Matsuda,M., Koseki,H., Wakana,S., Kawamoto,H. and
            Yoshida,H.
  TITLE     A novel gene essential for the development of single positive
            thymocytes
  JOURNAL   Mol Cell Biol 29 (18), 5128-5135 (2009)
   PUBMED   19620281
REFERENCE   8  (bases 1 to 5085)
  AUTHORS   Fu,G., Vallee,S., Rybakin,V., McGuire,M.V., Ampudia,J.,
            Brockmeyer,C., Salek,M., Fallen,P.R., Hoerter,J.A., Munshi,A.,
            Huang,Y.H., Hu,J., Fox,H.S., Sauer,K., Acuto,O. and Gascoigne,N.R.
  TITLE     Themis controls thymocyte selection through regulation of T cell
            antigen receptor-mediated signaling
  JOURNAL   Nat Immunol 10 (8), 848-856 (2009)
   PUBMED   19597499
  REMARK    GeneRIF: Themis had high expression in thymocytes between the pre-T
            cell antigen receptor and positive-selection checkpoints and low
            expression in mature T cells. Themis-deficient T-cells showed
            defective positive selection, resulting in fewer mature thymocytes.
REFERENCE   9  (bases 1 to 5085)
  AUTHORS   Lesourne,R., Uehara,S., Lee,J., Song,K.D., Li,L., Pinkhasov,J.,
            Zhang,Y., Weng,N.P., Wildt,K.F., Wang,L., Bosselut,R. and Love,P.E.
  TITLE     Themis, a T cell-specific protein important for late thymocyte
            development
  JOURNAL   Nat Immunol 10 (8), 840-847 (2009)
   PUBMED   19597498
  REMARK    GeneRIF: Themis functions to sustain and/or integrate signals
            required for proper lineage commitment and maturation.
            Erratum:[Nat Immunol. 2010 Jan;11(1):97]
REFERENCE   10 (bases 1 to 5085)
  AUTHORS   Johnson,A.L., Aravind,L., Shulzhenko,N., Morgun,A., Choi,S.Y.,
            Crockford,T.L., Lambe,T., Domaschenz,H., Kucharska,E.M., Zheng,L.,
            Vinuesa,C.G., Lenardo,M.J., Goodnow,C.C., Cornall,R.J. and
            Schwartz,R.H.
  TITLE     Themis is a member of a new metazoan gene family and is required
            for the completion of thymocyte positive selection
  JOURNAL   Nat Immunol 10 (8), 831-839 (2009)
   PUBMED   19597497
  REMARK    GeneRIF: Themis plays a unique role in sustaining positive
            selection
            GeneRIF: Themis is a member of a new metazoan gene family and is
            required for the completion of thymocyte positive selection
            Erratum:[Nat Immunol. 2010 Jan;11(1):97]
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BB638055.1, AK040594.1,
            AK169558.1 and AA967480.1.
            
            On Mar 26, 2015 this sequence version replaced NM_178666.5.
            
            Summary: This gene encodes a protein that plays a regulatory role
            in both positive and negative T-cell selection during late
            thymocyte development. The protein functions through T-cell antigen
            receptor signaling, and is necessary for proper lineage commitment
            and maturation of T-cells. Alternative splicing results in multiple
            transcript variants. [provided by RefSeq, Mar 2015].
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK088136.1, AK041562.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849383, SAMN00849388
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-37                BB638055.1         2-38
            38-2231             AK040594.1         2-2195
            2232-4024           AK169558.1         1975-3767
            4025-4025           AA967480.1         345-345
            4026-5085           AK169558.1         3769-4828
FEATURES             Location/Qualifiers
     source          1..5085
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="10"
                     /map="10 16.06 cM"
     gene            1..5085
                     /gene="Themis"
                     /gene_synonym="E430004N04Rik; Gasp; Spot; thylex; Tsepa"
                     /note="thymocyte selection associated"
                     /db_xref="GeneID:210757"
                     /db_xref="MGI:MGI:2443552"
     exon            1..391
                     /gene="Themis"
                     /gene_synonym="E430004N04Rik; Gasp; Spot; thylex; Tsepa"
                     /inference="alignment:Splign:2.1.0"
     CDS             301..2211
                     /gene="Themis"
                     /gene_synonym="E430004N04Rik; Gasp; Spot; thylex; Tsepa"
                     /note="isoform 1 is encoded by transcript variant 1;
                     protein THEMIS; thymus lymphocyte exclusively expressed;
                     thymocyte-expressed molecule involved in selection;
                     grb2-associating protein; thymocyte selection pathway
                     associated; signaling phosphoprotein specific for T cells"
                     /codon_start=1
                     /product="protein THEMIS isoform 1"
                     /protein_id="NP_848781.1"
                     /db_xref="CCDS:CCDS23758.1"
                     /db_xref="GeneID:210757"
                     /db_xref="MGI:MGI:2443552"
                     /translation="MALSLEEFVYSLDLRTLPRVLEIQSGIYFEGSVYEMFGNECCLS
                     TGEVIKITGLKIKKMMAEICEGAIGGCESQKPFELPMNFPGLFKVMADKTPYLSIEEI
                     TRTVNIGPSRLGHPCFYHLKDIKLENLIIKQGEPIRFNSVEEINGETLVNCGVVRNHQ
                     SHSFTLPLSQEGEFYECEDEHIYTLKEIVEWKIPKNRTRTVKLTDFSNKWDSTNPFPE
                     DFYGTLILKPVYEIQGVLKFQKDIVRILPSLDVEVKDITDSYDANWFLQLLSTDDLFE
                     MTSKEFPVVAEVVEISQGNHLPQSILQREKTIVIHKKYQASRILASEIRSNFPKRHFL
                     IPISYKGKFKRRPREFPTAYDLQIAKSRKETLHVVATKAFHTLHKELSPVSVGDQFLV
                     HHSETTEVVFEGTRKVNVLTCEKVLNKTREDAQLPLYMEGGFVEVIHDKKQYQISELC
                     TQFCWPFNVKVAVRDLSIKDDILAATPGLQLEEDITDSYLLISDFANPEECWEIPMSR
                     LNMTVRLVNGSSLPADAGLLQVRSFVEEITEEQYYMMRRYESSLSHPPPRPPKHPSAE
                     EMKLTLLSLAEERTINLPKSLKSHHVDRPKKLPSDESGQDSRAPVGFQNDVADVERQK
                     SKHGPLQPQAPL"
     exon            392..553
                     /gene="Themis"
                     /gene_synonym="E430004N04Rik; Gasp; Spot; thylex; Tsepa"
                     /inference="alignment:Splign:2.1.0"
     exon            554..1012
                     /gene="Themis"
                     /gene_synonym="E430004N04Rik; Gasp; Spot; thylex; Tsepa"
                     /inference="alignment:Splign:2.1.0"
     exon            1013..2064
                     /gene="Themis"
                     /gene_synonym="E430004N04Rik; Gasp; Spot; thylex; Tsepa"
                     /inference="alignment:Splign:2.1.0"
     exon            2065..2217
                     /gene="Themis"
                     /gene_synonym="E430004N04Rik; Gasp; Spot; thylex; Tsepa"
                     /inference="alignment:Splign:2.1.0"
     exon            2218..5085
                     /gene="Themis"
                     /gene_synonym="E430004N04Rik; Gasp; Spot; thylex; Tsepa"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2813..2818
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Themis"
                     /gene_synonym="E430004N04Rik; Gasp; Spot; thylex; Tsepa"
                     /note="hexamer: AATAAA"
     polyA_site      2840
                     /gene="Themis"
                     /gene_synonym="E430004N04Rik; Gasp; Spot; thylex; Tsepa"
                     /note="major polyA site"
     regulatory      3987..3992
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Themis"
                     /gene_synonym="E430004N04Rik; Gasp; Spot; thylex; Tsepa"
                     /note="hexamer: AATAAA"
     polyA_site      4009
                     /gene="Themis"
                     /gene_synonym="E430004N04Rik; Gasp; Spot; thylex; Tsepa"
                     /note="major polyA site"
     regulatory      5059..5064
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Themis"
                     /gene_synonym="E430004N04Rik; Gasp; Spot; thylex; Tsepa"
                     /note="hexamer: AATAAA"
     polyA_site      5085
                     /gene="Themis"
                     /gene_synonym="E430004N04Rik; Gasp; Spot; thylex; Tsepa"
                     /note="major polyA site"
ORIGIN      
        1 agggagaaca aaaagagctt tacccactgt ctcctgccta ttctcaagtt cttccgctgc
       61 ttgagctgtt tgccctgggc ttggctttcc tgtgacctcc ctgcaccagg tagaaactga
      121 agaccagcta ctgcagacaa gtacctcccc accttagggc tgtcctaccc cgattggctc
      181 ccatttcaca ttctgcctgt gtgggcaatt atgagcaaag acagaacggt agacaattga
      241 catctagaaa ccacaaggtc ttgtaaaaga tcaggtctat ataccaccga agacacaaca
      301 atggctttat ctctggaaga atttgtctac tcccttgacc tgaggaccct ccccagggtc
      361 ctagaaatcc agtcaggcat ctactttgaa gggtctgttt atgagatgtt tggcaatgaa
      421 tgctgtctgt caacaggaga agtgattaaa atcaccggtc tcaaaattaa gaagatgatg
      481 gctgaaattt gtgagggcgc tattggaggt tgtgagtctc agaagccttt tgagctgccc
      541 atgaattttc caggtctctt taaggtcatg gctgataaaa ctccatacct gtcaattgaa
      601 gaaatcacaa gaaccgttaa tattggaccc agcagactag gtcatccttg cttttatcac
      661 ctgaaggata taaaactaga gaatctcatt attaagcagg gtgagccaat ccggttcaac
      721 tcagttgaag agattaatgg agaaacgctg gtgaattgtg gtgtggtaag gaatcatcag
      781 agccactcat ttactttgcc attgtcacaa gaaggagaat tttatgagtg tgaggatgaa
      841 catatctata ccctaaaaga aattgttgaa tggaagattc ccaagaacag aacacggaca
      901 gtgaaactta cagatttctc gaataagtgg gactcaacca atccattccc agaagacttt
      961 tatggtacac tgattctcaa acctgtttat gaaatccaag gtgtgctgaa atttcaaaag
     1021 gacatagtcc ggattctccc aagtttagac gttgaagtca aagatattac tgattcttat
     1081 gatgctaact ggtttcttca gttgctgtct acggacgacc tttttgaaat gaccagcaaa
     1141 gagtttcctg tagtagctga agttgtcgaa atatctcaag ggaaccacct gccccaaagt
     1201 attttgcagc gagagaaaac cattgtcatc cacaaaaagt accaggcttc gaggatctta
     1261 gcttcagaaa ttcgcagcaa tttccctaaa agacacttct tgatccctat tagctacaaa
     1321 ggcaagttca aaagaagacc acgggagttc cccacggcct atgacctgca gatagctaag
     1381 agcaggaaag aaactctcca cgtggtggcc accaaagctt tccatacact tcacaaggag
     1441 ctgtcccctg tgtctgttgg agaccagttt ctagtgcatc attcagagac cacagaagtt
     1501 gtctttgagg ggacaagaaa agtaaacgtt ctgacctgtg aaaaagtcct caataagacc
     1561 cgtgaagatg cacagcttcc tctatacatg gaaggaggtt ttgtagaggt gattcatgat
     1621 aagaaacagt atcagatttc cgagctctgt acccagtttt gctggccctt caatgtgaag
     1681 gtggctgtga gagatctctc cattaaagat gacattttgg cagctacccc aggacttcag
     1741 ttagaggagg acatcacaga ttcttatcta cttatcagtg actttgcaaa tcctgaggaa
     1801 tgttgggaaa ttcccatgag ccgcttgaat atgactgttc gactagttaa tggtagcagt
     1861 ttgcctgcag atgcagggct acttcaagtc aggagttttg tagaagagat tacagaagaa
     1921 cagtattaca tgatgaggag gtacgaaagt tctctatcac atcccccacc tcgccctccc
     1981 aaacatccct cagctgagga aatgaagctc accttgctca gcttagcaga agagagaaca
     2041 ataaatctgc ccaagtctct caagagtcac catgtagaca gacccaagaa gcttccctca
     2101 gatgaaagtg gccaggattc aagagctcca gttggttttc agaatgatgt ggctgatgtg
     2161 gagagacaga agagcaagca tgggccactg cagccgcaag caccactgtg actacataac
     2221 tcttcaaaac tgaaaaacat caaaaataac aagacgtgat ggaagccact tgggcagtaa
     2281 acataaatgt tgcaatgaag aagaaagtct agccttccag ctgaaaaaca gcaattcccc
     2341 aatggactct ggcagaatct ggactggcca ctgcaaagac aaaaacaagc ttaaatgttt
     2401 tatcatatag agctcaaaga gagaaccatg atatggaatt taaattgtct atttggtttc
     2461 ttaaaaccca caagtgtggt tttcttgttg tttaagacat atttatttaa tatgcatttt
     2521 tatgtagctt gtttttgcta tcaacaattg gcaattagaa ataattttgt acttcaatta
     2581 ttcagtagaa tcctattttt gaaagtctaa actctcactg aatgttcacc tttatttcca
     2641 agcagttttc tttccatttt tccaggttca tcatcatttc tgctaaactc aataaggaaa
     2701 atgaattaca tctgacagtg acttaaaatg gccaagtatc ttctctgatt ccttgggaac
     2761 agtgaaaata agtttgactt cacattttca atttgtttta taatacaatg taaataaatg
     2821 tcaactccca acaactggca ccattatgag tcacagaacc ttctctaacc tcctccctcc
     2881 acttctaaaa gggagagttg ctgaccaaag aatcacccag gttctgaaaa tttgccttgt
     2941 actttatata ctatattctt gatcttgctt ctaatgaatg cttattagga ttgtagtaaa
     3001 ccatttagtt aaccataata tttgatgaga agaaagacac aatataattg taatgagcct
     3061 tataaatata gttgacagaa gccaaagaga ttattaatga ataaataaca gaatagtact
     3121 tatagctata tgcctgtaaa aattaaaggc aaacatattt tataagtttt cttattatgt
     3181 gttaaatata cagaaataac attacttttg tctttcataa cgtgaagaga ctgagaattt
     3241 ttcaaggtag gttacataga gaatagtggg aaaccttagg tttttttcat aagtcagact
     3301 tttacaaaac tttactctga tctcataatt aatttataag ttggaaaaac gtatttgatg
     3361 atttagtact gaagagtctt taaagatagc aacctatgag attattttca tcatataatc
     3421 acagctagtg atcttttata taatgtttat atcctgattt cttatccttt tctctttaaa
     3481 taacaactaa tctttatttt aatgctttaa aaattgctca gtgacataac tgttaccata
     3541 ttatatgtca ataacaaaat tttaggagat gtattatgtg taattagtaa aatacaaggt
     3601 gattgttttt gataattttt tagtttagtt tacacacatt tgattagtaa atgagaaagt
     3661 tgatgtctct gcattttggt ttttagacac catagagaat catgacccaa ccacatatcc
     3721 taagaaagct atattatgga taatctggga ccagtacaat tttcagctaa gagtgagcac
     3781 tgaatatttt tagtttggta tatgagcaca gttgaatttg gactttttgt aagcccatgt
     3841 ctttgactag aaaatgagaa aatgaattct cttcctttag ttgtgaatca tcaaaaactt
     3901 tctaccatat atcatgtcta ccatttctat cttaatgaaa ttccccttgc ataatctgct
     3961 tctagttgtc ttggaatcaa aaatataata aactttatga cctgtccaag atcttgagaa
     4021 gttggaacaa aaattttcat tgttcatata tatgcatctc tggccatggt taactatgaa
     4081 ataagttact ttctataaca atttagcaaa aacatattat ttaactagaa ctgaaaccag
     4141 ttttcaccat tgaggaagat gatttttttt taaagccagg aagaaaaaga gtgtccccta
     4201 cattcctacc cactgtgggt ttgcaatcat gggcccagtc atagcaacaa aatttctcgg
     4261 cacagtcatg atgcataaag aggtgtcaat atgaccagtt actaaatcta ctaattaaaa
     4321 agctgacttc cctaatattg aatcattgaa gaagtttgaa ggatgctatc ttagataggg
     4381 ttactactac tttaatgaaa caccatggca aactgcaact tgggggagga aaggccttat
     4441 ttcactcact gttccatata tcagttcacc atcaaaagca gtgagggcag aaacctgaag
     4501 gcaggagcta gtgaagagac cacagaggct tgctgcttac tggtttactc cttatggttt
     4561 gcccaaggat gcccctcccc ccccacacac acacaatgag ctgggacctt cctcatgaag
     4621 agggcaaagt atcaggatag actccctctt gctctctaaa ctccatttta agttgcttgt
     4681 cctcagagtg acatggcctt cacccttggc atctagaaaa gtgaccacaa agtattatca
     4741 ggagttgaaa atagacataa aacctttggc tagctcataa taacaagata gattggtcac
     4801 aataacagga tgatttcaga aaacctgagt cattcccagg tcaagagccc cttagatgta
     4861 cactctctcc ttgtggtttg aacagatgat ttgcaccaat cagtttaaag gtcaatattt
     4921 ctttactcat ttacccaatc atgtaacacc aaggcttgaa agctcctgct tgcaattttt
     4981 tgcctatata aatcctaaac tcctagacct ttcttaatct gttgcatcag gaaggtttgt
     5041 gttccgagtc caaatctgaa taaagacttt catgtgctta cattg
//
