LOCUS       NM_001305663            5094 bp    mRNA    linear   ROD 09-FEB-2025
DEFINITION  Mus musculus thymocyte selection associated (Themis), transcript
            variant 2, mRNA.
ACCESSION   NM_001305663
VERSION     NM_001305663.1
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 5094)
  AUTHORS   Risemberg,E.L., Smeekens,J.M., Cruz Cisneros,M.C., Hampton,B.K.,
            Hock,P., Linnertz,C.L., Miller,D.R., Orgel,K., Shaw,G.D., de
            Villena,F.P.M., Burks,A.W., Valdar,W., Kulis,M.D. and Ferris,M.T.
  TITLE     A mutation in Themis contributes to anaphylaxis severity following
            oral peanut challenge in CC027 mice
  JOURNAL   J Allergy Clin Immunol 154 (2), 387-397 (2024)
   PUBMED   38670234
REFERENCE   2  (bases 1 to 5094)
  AUTHORS   Melique,S., Vadel,A., Rouquie,N., Yang,C., Bories,C., Cotineau,C.,
            Saoudi,A., Fazilleau,N. and Lesourne,R.
  TITLE     THEMIS promotes T cell development and maintenance by rising the
            signaling threshold of the inhibitory receptor BTLA
  JOURNAL   Proc Natl Acad Sci U S A 121 (20), e2318773121 (2024)
   PUBMED   38713628
  REMARK    GeneRIF: THEMIS promotes T cell development and maintenance by
            rising the signaling threshold of the inhibitory receptor BTLA.
REFERENCE   3  (bases 1 to 5094)
  AUTHORS   Marrocco,R., Bernard,I., Joulia,E., Barascud,R., Dejean,A.S.,
            Lesourne,R. and Saoudi,A.
  TITLE     Positive regulation of Vav1 by Themis controls CD4 T cell
            pathogenicity in a mouse model of central nervous system
            inflammation
  JOURNAL   Cell Mol Life Sci 81 (1), 161 (2024)
   PUBMED   38565808
  REMARK    GeneRIF: Positive regulation of Vav1 by Themis controls CD4 T cell
            pathogenicity in a mouse model of central nervous system
            inflammation.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 5094)
  AUTHORS   Gautam,N., Wojciech,L., Yap,J., Chua,Y.L., Ding,E.M., Sim,D.C.,
            Tan,A.S., Ahl,P.J., Prasad,M., Tung,D.W., Connolly,J.E.,
            Adriani,G., Brzostek,J. and Gascoigne,N.R.
  TITLE     Themis controls T cell activation, effector functions, and
            metabolism of peripheral CD8+ T cells
  JOURNAL   Life Sci Alliance 6 (12), e202302156 (2023)
   PUBMED   37739454
  REMARK    GeneRIF: Themis controls T cell activation, effector functions, and
            metabolism of peripheral CD8[+] T cells.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 5094)
  AUTHORS   Choi,S., Hatzihristidis,T., Gaud,G., Dutta,A., Lee,J., Arya,A.,
            Clubb,L.M., Stamos,D.B., Markovics,A., Mikecz,K. and Love,P.
  TITLE     GRB2 promotes thymocyte positive selection by facilitating
            THEMIS-mediated inactivation of SHP1
  JOURNAL   J Exp Med 220 (7) (2023)
   PUBMED   37067793
REFERENCE   6  (bases 1 to 5094)
  AUTHORS   Patrick,M.S., Oda,H., Hayakawa,K., Sato,Y., Eshima,K., Kirikae,T.,
            Iemura,S., Shirai,M., Abe,T., Natsume,T., Sasazuki,T. and Suzuki,H.
  TITLE     Gasp, a Grb2-associating protein, is critical for positive
            selection of thymocytes
  JOURNAL   Proc Natl Acad Sci U S A 106 (38), 16345-16350 (2009)
   PUBMED   19805304
  REMARK    GeneRIF: Study described a gene called Gasp that is critical for
            positive selection.
            GeneRIF: Gasp associates with Grb2 and is required for positive
            selection of thymocytes
REFERENCE   7  (bases 1 to 5094)
  AUTHORS   Kakugawa,K., Yasuda,T., Miura,I., Kobayashi,A., Fukiage,H.,
            Satoh,R., Matsuda,M., Koseki,H., Wakana,S., Kawamoto,H. and
            Yoshida,H.
  TITLE     A novel gene essential for the development of single positive
            thymocytes
  JOURNAL   Mol Cell Biol 29 (18), 5128-5135 (2009)
   PUBMED   19620281
REFERENCE   8  (bases 1 to 5094)
  AUTHORS   Fu,G., Vallee,S., Rybakin,V., McGuire,M.V., Ampudia,J.,
            Brockmeyer,C., Salek,M., Fallen,P.R., Hoerter,J.A., Munshi,A.,
            Huang,Y.H., Hu,J., Fox,H.S., Sauer,K., Acuto,O. and Gascoigne,N.R.
  TITLE     Themis controls thymocyte selection through regulation of T cell
            antigen receptor-mediated signaling
  JOURNAL   Nat Immunol 10 (8), 848-856 (2009)
   PUBMED   19597499
  REMARK    GeneRIF: Themis had high expression in thymocytes between the pre-T
            cell antigen receptor and positive-selection checkpoints and low
            expression in mature T cells. Themis-deficient T-cells showed
            defective positive selection, resulting in fewer mature thymocytes.
REFERENCE   9  (bases 1 to 5094)
  AUTHORS   Lesourne,R., Uehara,S., Lee,J., Song,K.D., Li,L., Pinkhasov,J.,
            Zhang,Y., Weng,N.P., Wildt,K.F., Wang,L., Bosselut,R. and Love,P.E.
  TITLE     Themis, a T cell-specific protein important for late thymocyte
            development
  JOURNAL   Nat Immunol 10 (8), 840-847 (2009)
   PUBMED   19597498
  REMARK    GeneRIF: Themis functions to sustain and/or integrate signals
            required for proper lineage commitment and maturation.
            Erratum:[Nat Immunol. 2010 Jan;11(1):97]
REFERENCE   10 (bases 1 to 5094)
  AUTHORS   Johnson,A.L., Aravind,L., Shulzhenko,N., Morgun,A., Choi,S.Y.,
            Crockford,T.L., Lambe,T., Domaschenz,H., Kucharska,E.M., Zheng,L.,
            Vinuesa,C.G., Lenardo,M.J., Goodnow,C.C., Cornall,R.J. and
            Schwartz,R.H.
  TITLE     Themis is a member of a new metazoan gene family and is required
            for the completion of thymocyte positive selection
  JOURNAL   Nat Immunol 10 (8), 831-839 (2009)
   PUBMED   19597497
  REMARK    GeneRIF: Themis plays a unique role in sustaining positive
            selection
            GeneRIF: Themis is a member of a new metazoan gene family and is
            required for the completion of thymocyte positive selection
            Erratum:[Nat Immunol. 2010 Jan;11(1):97]
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BB638055.1, AK040594.1,
            AK169558.1, AC159472.6 and AC153798.1.
            
            Summary: This gene encodes a protein that plays a regulatory role
            in both positive and negative T-cell selection during late
            thymocyte development. The protein functions through T-cell antigen
            receptor signaling, and is necessary for proper lineage commitment
            and maturation of T-cells. Alternative splicing results in multiple
            transcript variants. [provided by RefSeq, Mar 2015].
            
            Transcript Variant: This variant (2) uses an alternate 5' exon and
            lacks a 3' coding exon compared to variant 1. This variant
            represents translation initiation at a downstream AUG compared to
            variant 1; the 5'-most initiation codon, as used in variant 1, is
            associated with a truncated ORF that would render the transcript a
            candidate for nonsense-mediated decay (NMD). The encoded isoform
            (2) has a shorter N-terminus and a shorter and distinct C-terminus,
            compared to isoform 1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK169558.1 [ECO:0000332]
            RNAseq introns              :: partial sample support SAMN00849383,
                                           SAMN00849385 [ECO:0000350]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-37                BB638055.1         2-38
            38-267              AK040594.1         2-231
            268-916             AK169558.1         2-650
            917-917             AC159472.6         106345-106345       c
            918-4033            AK169558.1         652-3767
            4034-4034           AC153798.1         92747-92747         c
            4035-5094           AK169558.1         3769-4828
FEATURES             Location/Qualifiers
     source          1..5094
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="10"
                     /map="10 16.06 cM"
     gene            1..5094
                     /gene="Themis"
                     /gene_synonym="E430004N04Rik; Gasp; Spot; thylex; Tsepa"
                     /note="thymocyte selection associated"
                     /db_xref="GeneID:210757"
                     /db_xref="MGI:MGI:2443552"
     exon            1..391
                     /gene="Themis"
                     /gene_synonym="E430004N04Rik; Gasp; Spot; thylex; Tsepa"
                     /inference="alignment:Splign:2.1.0"
     exon            392..553
                     /gene="Themis"
                     /gene_synonym="E430004N04Rik; Gasp; Spot; thylex; Tsepa"
                     /inference="alignment:Splign:2.1.0"
     exon            554..715
                     /gene="Themis"
                     /gene_synonym="E430004N04Rik; Gasp; Spot; thylex; Tsepa"
                     /inference="alignment:Splign:2.1.0"
     exon            716..1174
                     /gene="Themis"
                     /gene_synonym="E430004N04Rik; Gasp; Spot; thylex; Tsepa"
                     /inference="alignment:Splign:2.1.0"
     CDS             730..2268
                     /gene="Themis"
                     /gene_synonym="E430004N04Rik; Gasp; Spot; thylex; Tsepa"
                     /note="isoform 2 is encoded by transcript variant 2;
                     protein THEMIS; thymus lymphocyte exclusively expressed;
                     thymocyte-expressed molecule involved in selection;
                     grb2-associating protein; thymocyte selection pathway
                     associated; signaling phosphoprotein specific for T cells"
                     /codon_start=1
                     /product="protein THEMIS isoform 2"
                     /protein_id="NP_001292592.1"
                     /db_xref="GeneID:210757"
                     /db_xref="MGI:MGI:2443552"
                     /translation="MADKTPYLSIEEITRTVNIGPSRLGHPCFYHLKDIKLENLIIKQ
                     GEPIRFNSVEEINGETLVNCGVVRNHQSHSFTLPLSQEGEFYECEDEHIYTLKEIVEW
                     KIPKNRTRTVKLTDFSNKWDSTNPFPEDFYGTLILKPVYEIQGVLKFQKDIVRILPSL
                     DVEVKDITDSYDANWFLQLLSTDDLFEMTSKEFPVVAEVVEISQGNHLPQSILQREKT
                     IVIHKKYQASRILASEIRSNFPKRHFLIPISYKGKFKRRPREFPTAYDLQIAKSRKET
                     LHVVATKAFHTLHKELSPVSVGDQFLVHHSETTEVVFEGTRKVNVLTCEKVLNKTRED
                     AQLPLYMEGGFVEVIHDKKQYQISELCTQFCWPFNVKVAVRDLSIKDDILAATPGLQL
                     EEDITDSYLLISDFANPEECWEIPMSRLNMTVRLVNGSSLPADAGLLQVRSFVEEITE
                     EQYYMMRRYESSLSHPPPRPPKHPSAEEMKLTLLSLAEERTINLPKSLKNSSKLKNIK
                     NNKT"
     exon            1175..2226
                     /gene="Themis"
                     /gene_synonym="E430004N04Rik; Gasp; Spot; thylex; Tsepa"
                     /inference="alignment:Splign:2.1.0"
     exon            2227..5094
                     /gene="Themis"
                     /gene_synonym="E430004N04Rik; Gasp; Spot; thylex; Tsepa"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2822..2827
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Themis"
                     /gene_synonym="E430004N04Rik; Gasp; Spot; thylex; Tsepa"
                     /note="hexamer: AATAAA"
     polyA_site      2849
                     /gene="Themis"
                     /gene_synonym="E430004N04Rik; Gasp; Spot; thylex; Tsepa"
                     /note="major polyA site"
     regulatory      3996..4001
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Themis"
                     /gene_synonym="E430004N04Rik; Gasp; Spot; thylex; Tsepa"
                     /note="hexamer: AATAAA"
     polyA_site      4018
                     /gene="Themis"
                     /gene_synonym="E430004N04Rik; Gasp; Spot; thylex; Tsepa"
                     /note="major polyA site"
     regulatory      5068..5073
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Themis"
                     /gene_synonym="E430004N04Rik; Gasp; Spot; thylex; Tsepa"
                     /note="hexamer: AATAAA"
     polyA_site      5094
                     /gene="Themis"
                     /gene_synonym="E430004N04Rik; Gasp; Spot; thylex; Tsepa"
                     /note="major polyA site"
ORIGIN      
        1 agggagaaca aaaagagctt tacccactgt ctcctgccta ttctcaagtt cttccgctgc
       61 ttgagctgtt tgccctgggc ttggctttcc tgtgacctcc ctgcaccagg tagaaactga
      121 agaccagcta ctgcagacaa gtacctcccc accttagggc tgtcctaccc cgattggctc
      181 ccatttcaca ttctgcctgt gtgggcaatt atgagcaaag acagaacggt agacaattga
      241 catctagaaa ccacaaggtc ttgtaaaaga tcaggtctat ataccaccga agacacaaca
      301 atggctttat ctctggaaga atttgtctac tcccttgacc tgaggaccct ccccagggtc
      361 ctagaaatcc agtcaggcat ctactttgaa gggtctgttt atgagatgtt tggcaatgaa
      421 tgctgtctgt caacaggaga agtgattaaa atcaccggtc tcaaaattaa gaagatgatg
      481 gctgaaattt gtgagggcgc tattggaggt tgtgagtctc agaagccttt tgagctgccc
      541 atgaattttc cagatgttct acatgaacaa atgtgctatc tgtgaacaaa gaaacttttg
      601 tttgagccta ctactattgg tgaccagtgc gattttgaaa ataagacgaa agaagttgtg
      661 tgtgccttta ctaccatcta attagcaagc tctccttgtg ttctaacttg ctgaggtctc
      721 tttaaggtca tggctgataa aactccatac ctgtcaattg aagaaatcac aagaaccgtt
      781 aatattggac ccagcagact aggtcatcct tgcttttatc acctgaagga tataaaacta
      841 gagaatctca ttattaagca gggtgagcca atccggttca actcagttga agagattaat
      901 ggagaaacgc tggtgaattg tggtgtggta aggaatcatc agagccactc atttactttg
      961 ccattgtcac aagaaggaga attttatgag tgtgaggatg aacatatcta taccctaaaa
     1021 gaaattgttg aatggaagat tcccaagaac agaacacgga cagtgaaact tacagatttc
     1081 tcgaataagt gggactcaac caatccattc ccagaagact tttatggtac actgattctc
     1141 aaacctgttt atgaaatcca aggtgtgctg aaatttcaaa aggacatagt ccggattctc
     1201 ccaagtttag acgttgaagt caaagatatt actgattctt atgatgctaa ctggtttctt
     1261 cagttgctgt ctacggacga cctttttgaa atgaccagca aagagtttcc tgtagtagct
     1321 gaagttgtcg aaatatctca agggaaccac ctgccccaaa gtattttgca gcgagagaaa
     1381 accattgtca tccacaaaaa gtaccaggct tcgaggatct tagcttcaga aattcgcagc
     1441 aatttcccta aaagacactt cttgatccct attagctaca aaggcaagtt caaaagaaga
     1501 ccacgggagt tccccacggc ctatgacctg cagatagcta agagcaggaa agaaactctc
     1561 cacgtggtgg ccaccaaagc tttccataca cttcacaagg agctgtcccc tgtgtctgtt
     1621 ggagaccagt ttctagtgca tcattcagag accacagaag ttgtctttga ggggacaaga
     1681 aaagtaaacg ttctgacctg tgaaaaagtc ctcaataaga cccgtgaaga tgcacagctt
     1741 cctctataca tggaaggagg ttttgtagag gtgattcatg ataagaaaca gtatcagatt
     1801 tccgagctct gtacccagtt ttgctggccc ttcaatgtga aggtggctgt gagagatctc
     1861 tccattaaag atgacatttt ggcagctacc ccaggacttc agttagagga ggacatcaca
     1921 gattcttatc tacttatcag tgactttgca aatcctgagg aatgttggga aattcccatg
     1981 agccgcttga atatgactgt tcgactagtt aatggtagca gtttgcctgc agatgcaggg
     2041 ctacttcaag tcaggagttt tgtagaagag attacagaag aacagtatta catgatgagg
     2101 aggtacgaaa gttctctatc acatccccca cctcgccctc ccaaacatcc ctcagctgag
     2161 gaaatgaagc tcaccttgct cagcttagca gaagagagaa caataaatct gcccaagtct
     2221 ctcaagaact cttcaaaact gaaaaacatc aaaaataaca agacgtgatg gaagccactt
     2281 gggcagtaaa cataaatgtt gcaatgaaga agaaagtcta gccttccagc tgaaaaacag
     2341 caattcccca atggactctg gcagaatctg gactggccac tgcaaagaca aaaacaagct
     2401 taaatgtttt atcatataga gctcaaagag agaaccatga tatggaattt aaattgtcta
     2461 tttggtttct taaaacccac aagtgtggtt ttcttgttgt ttaagacata tttatttaat
     2521 atgcattttt atgtagcttg tttttgctat caacaattgg caattagaaa taattttgta
     2581 cttcaattat tcagtagaat cctatttttg aaagtctaaa ctctcactga atgttcacct
     2641 ttatttccaa gcagttttct ttccattttt ccaggttcat catcatttct gctaaactca
     2701 ataaggaaaa tgaattacat ctgacagtga cttaaaatgg ccaagtatct tctctgattc
     2761 cttgggaaca gtgaaaataa gtttgacttc acattttcaa tttgttttat aatacaatgt
     2821 aaataaatgt caactcccaa caactggcac cattatgagt cacagaacct tctctaacct
     2881 cctccctcca cttctaaaag ggagagttgc tgaccaaaga atcacccagg ttctgaaaat
     2941 ttgccttgta ctttatatac tatattcttg atcttgcttc taatgaatgc ttattaggat
     3001 tgtagtaaac catttagtta accataatat ttgatgagaa gaaagacaca atataattgt
     3061 aatgagcctt ataaatatag ttgacagaag ccaaagagat tattaatgaa taaataacag
     3121 aatagtactt atagctatat gcctgtaaaa attaaaggca aacatatttt ataagttttc
     3181 ttattatgtg ttaaatatac agaaataaca ttacttttgt ctttcataac gtgaagagac
     3241 tgagaatttt tcaaggtagg ttacatagag aatagtggga aaccttaggt ttttttcata
     3301 agtcagactt ttacaaaact ttactctgat ctcataatta atttataagt tggaaaaacg
     3361 tatttgatga tttagtactg aagagtcttt aaagatagca acctatgaga ttattttcat
     3421 catataatca cagctagtga tcttttatat aatgtttata tcctgatttc ttatcctttt
     3481 ctctttaaat aacaactaat ctttatttta atgctttaaa aattgctcag tgacataact
     3541 gttaccatat tatatgtcaa taacaaaatt ttaggagatg tattatgtgt aattagtaaa
     3601 atacaaggtg attgtttttg ataatttttt agtttagttt acacacattt gattagtaaa
     3661 tgagaaagtt gatgtctctg cattttggtt tttagacacc atagagaatc atgacccaac
     3721 cacatatcct aagaaagcta tattatggat aatctgggac cagtacaatt ttcagctaag
     3781 agtgagcact gaatattttt agtttggtat atgagcacag ttgaatttgg actttttgta
     3841 agcccatgtc tttgactaga aaatgagaaa atgaattctc ttcctttagt tgtgaatcat
     3901 caaaaacttt ctaccatata tcatgtctac catttctatc ttaatgaaat tccccttgca
     3961 taatctgctt ctagttgtct tggaatcaaa aatataataa actttatgac ctgtccaaga
     4021 tcttgagaag ttggaacaaa aattttcatt gttcatatat atgcatctct ggccatggtt
     4081 aactatgaaa taagttactt tctataacaa tttagcaaaa acatattatt taactagaac
     4141 tgaaaccagt tttcaccatt gaggaagatg attttttttt aaagccagga agaaaaagag
     4201 tgtcccctac attcctaccc actgtgggtt tgcaatcatg ggcccagtca tagcaacaaa
     4261 atttctcggc acagtcatga tgcataaaga ggtgtcaata tgaccagtta ctaaatctac
     4321 taattaaaaa gctgacttcc ctaatattga atcattgaag aagtttgaag gatgctatct
     4381 tagatagggt tactactact ttaatgaaac accatggcaa actgcaactt gggggaggaa
     4441 aggccttatt tcactcactg ttccatatat cagttcacca tcaaaagcag tgagggcaga
     4501 aacctgaagg caggagctag tgaagagacc acagaggctt gctgcttact ggtttactcc
     4561 ttatggtttg cccaaggatg cccctccccc cccacacaca cacaatgagc tgggaccttc
     4621 ctcatgaaga gggcaaagta tcaggataga ctccctcttg ctctctaaac tccattttaa
     4681 gttgcttgtc ctcagagtga catggccttc acccttggca tctagaaaag tgaccacaaa
     4741 gtattatcag gagttgaaaa tagacataaa acctttggct agctcataat aacaagatag
     4801 attggtcaca ataacaggat gatttcagaa aacctgagtc attcccaggt caagagcccc
     4861 ttagatgtac actctctcct tgtggtttga acagatgatt tgcaccaatc agtttaaagg
     4921 tcaatatttc tttactcatt tacccaatca tgtaacacca aggcttgaaa gctcctgctt
     4981 gcaatttttt gcctatataa atcctaaact cctagacctt tcttaatctg ttgcatcagg
     5041 aaggtttgtg ttccgagtcc aaatctgaat aaagactttc atgtgcttac attg
//
