LOCUS       NM_011581               5922 bp    mRNA    linear   ROD 24-SEP-2024
DEFINITION  Mus musculus thrombospondin 2 (Thbs2), mRNA.
ACCESSION   NM_011581
VERSION     NM_011581.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 5922)
  AUTHORS   Shu,H., Parada,I., Delgado,A., Prince,D.A. and Gu,F.
  TITLE     Increased excitatory connectivity and epileptiform activity in
            thrombospondin1/2 knockout mice following cortical trauma
  JOURNAL   Neurobiol Dis 200, 106634 (2024)
   PUBMED   39122122
  REMARK    GeneRIF: Increased excitatory connectivity and epileptiform
            activity in thrombospondin1/2 knockout mice following cortical
            trauma.
REFERENCE   2  (bases 1 to 5922)
  AUTHORS   Hadar,N., Porgador,O., Cohen,I., Levi,H., Dolgin,V., Yogev,Y.,
            Sued-Hendrickson,S., Shelef,I., Didkovsky,E., Eskin-Schwartz,M. and
            Birk,O.S.
  TITLE     Heterozygous THBS2 pathogenic variant causes Ehlers-Danlos syndrome
            with prominent vascular features in humans and mice
  JOURNAL   Eur J Hum Genet 32 (5), 550-557 (2024)
   PUBMED   38433265
  REMARK    GeneRIF: Heterozygous THBS2 pathogenic variant causes Ehlers-Danlos
            syndrome with prominent vascular features in humans and mice.
REFERENCE   3  (bases 1 to 5922)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   4  (bases 1 to 5922)
  AUTHORS   Chen,Z., Huang,Y., Xing,H., Tseng,T., Edelman,H., Perry,R. and
            Kyriakides,T.R.
  TITLE     Novel muscle-derived extracellular matrix hydrogel promotes
            angiogenesis and neurogenesis in volumetric muscle loss
  JOURNAL   Matrix Biol 127, 38-47 (2024)
   PUBMED   38325441
REFERENCE   5  (bases 1 to 5922)
  AUTHORS   Li,X., Ding,Y., Zhou,M., Zhou,Z., Xie,T., Wang,Y., Liu,Z., Si,Y.
            and Shi,Z.
  TITLE     Deficiency of thrombospondin-2 alleviates intimal hyperplasia in
            mice by modulating vascular smooth muscle cell proliferation and
            migration
  JOURNAL   FASEB J 37 (2), e22743 (2023)
   PUBMED   36645109
  REMARK    GeneRIF: Deficiency of thrombospondin-2 alleviates intimal
            hyperplasia in mice by modulating vascular smooth muscle cell
            proliferation and migration.
REFERENCE   6  (bases 1 to 5922)
  AUTHORS   Himmelbauer,H., Harvey,R.P., Copeland,N.G., Jenkins,N.A. and
            Silver,L.M.
  TITLE     High-resolution genetic analysis of a deletion on mouse chromosome
            17 extending over the fused, tufted, and homeobox Nkx2-5 loci
  JOURNAL   Mamm Genome 5 (12), 814-816 (1994)
   PUBMED   7894168
REFERENCE   7  (bases 1 to 5922)
  AUTHORS   Trachtulec,Z., Vincek,V., Hamvas,R.M., Forejt,J., Lehrach,H. and
            Klein,J.
  TITLE     Physical map of mouse chromosome 17 in the region relevant for
            positional cloning of the Hybrid sterility 1 gene
  JOURNAL   Genomics 23 (1), 132-137 (1994)
   PUBMED   7829061
REFERENCE   8  (bases 1 to 5922)
  AUTHORS   Laherty,C.D., O'Rourke,K., Wolf,F.W., Katz,R., Seldin,M.F. and
            Dixit,V.M.
  TITLE     Characterization of mouse thrombospondin 2 sequence and expression
            during cell growth and development
  JOURNAL   J Biol Chem 267 (5), 3274-3281 (1992)
   PUBMED   1371115
REFERENCE   9  (bases 1 to 5922)
  AUTHORS   Bornstein,P., Devarayalu,S., Li,P., Disteche,C.M. and Framson,P.
  TITLE     A second thrombospondin gene in the mouse is similar in
            organization to thrombospondin 1 but does not respond to serum
  JOURNAL   Proc Natl Acad Sci U S A 88 (19), 8636-8640 (1991)
   PUBMED   1924325
REFERENCE   10 (bases 1 to 5922)
  AUTHORS   Bornstein,P., O'Rourke,K., Wikstrom,K., Wolf,F.W., Katz,R., Li,P.
            and Dixit,V.M.
  TITLE     A second, expressed thrombospondin gene (Thbs2) exists in the mouse
            genome
  JOURNAL   J Biol Chem 266 (20), 12821-12824 (1991)
   PUBMED   1712771
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            CJ049934.1, AK132508.1, AK142552.1 and AK165881.1.
            
            On Jun 11, 2009 this sequence version replaced NM_011581.2.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK142552.1, L07803.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-170               CJ049934.1         2-171
            171-3044            AK132508.1         2-2875
            3045-4077           AK142552.1         3017-4049
            4078-5922           AK165881.1         695-2539
FEATURES             Location/Qualifiers
     source          1..5922
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="17"
                     /map="17 8.95 cM"
     gene            1..5922
                     /gene="Thbs2"
                     /gene_synonym="Thbs-2; TSP2"
                     /note="thrombospondin 2"
                     /db_xref="GeneID:21826"
                     /db_xref="MGI:MGI:98738"
     exon            1..293
                     /gene="Thbs2"
                     /gene_synonym="Thbs-2; TSP2"
                     /inference="alignment:Splign:2.1.0"
     exon            294..442
                     /gene="Thbs2"
                     /gene_synonym="Thbs-2; TSP2"
                     /inference="alignment:Splign:2.1.0"
     CDS             391..3909
                     /gene="Thbs2"
                     /gene_synonym="Thbs-2; TSP2"
                     /note="Thrombospondin-2"
                     /codon_start=1
                     /product="thrombospondin-2 precursor"
                     /protein_id="NP_035711.2"
                     /db_xref="CCDS:CCDS49958.1"
                     /db_xref="GeneID:21826"
                     /db_xref="MGI:MGI:98738"
                     /translation="MLWALALLALGIGPRASAGDHVKDTSFDLFSISNINRKTIGAKQ
                     FRGPDPGVPAYRFVRFDYIPPVNTDDLNRIVKLARRKEGFFLTAQLKQDRKSRGTLLV
                     LEGPGTSQRQFEIVSNGPGDTLDLNYWVEGNQHTNFLEDVGLADSQWKNVTVQVASDT
                     YSLYVGCDLIDSVTLEEPFYEQLEVDRSRMYVAKGASRESHFRGLLQNVHLVFADSVE
                     DILSKKGCQHSQGAEVNTISEHTETLHLSPHITTDLVVQGVEKAQEVCTHSCEELSNM
                     MNELSGLHVMVNQLSKNLERVSSDNQFLLELIGGPLKTRNMSACVQEGRIFAENETWV
                     VDSCTTCTCKKFKTVCHQITCSPATCANPSFVEGECCPSCSHSADSDEGWSPWAEWTE
                     CSVTCGSGTQQRGRSCDVTSNTCLGPSIQTRTCSLGKCDTRIRQNGGWSHWSPWSSCS
                     VTCGVGNVTRIRLCNSPVPQMGGKNCKGSGRETKPCQRDPCPIDGRWSPWSPWSACTV
                     TCAGGIRERSRVCNSPEPQYGGKDCVGDVTEHQMCNKRSCPIDGCLSNPCFPGAKCNS
                     FPDGSWSCGSCPVGFLGNGTHCEDLDECAVVTDICFSTNKAPRCVNTNPGFHCLPCPP
                     RYKGNQPFGVGLEDARTEKQVCEPENPCKDKTHSCHKNAECIYLGHFSDPMYKCECQI
                     GYAGDGLICGEDSDLDGWPNNNLVCATNATYHCIKDNCPKLPNSGQEDFDKDGIGDAC
                     DEDDDNDGVSDEKDNCQLLFNPRQLDYDKDEVGDRCDNCPYVHNPAQIDTDNNGEGDA
                     CSVDIDGDDVFNERDNCPYVYNTDQRDTDGDGVGDHCDNCPLMHNPDQIDQDNDLVGD
                     QCDNNEDIDDDGHQNNQDNCPYISNSNQADHDNDGKGDACDSDDDNDGVPDDRDNCRL
                     VFNPDQEDSDGDGRGDICKDDFDNDNVPDIDDVCPENNAITETDFRNFQMVPLDPKGT
                     TQIDPNWVIRHQGKELVQTANSDPGIAVGFDEFGSVDFSGTFYVNTDRDDDYAGFVFG
                     YQSSSRFYVVMWKQVTQTYWEDKPSRAYGYSGVSLKVVNSTTGTGEHLRNALWHTGNT
                     EGQVRTLWHDPKNIGWKDYTAYRWHLIHRPKTGYMRVLVHEGKQVMADSGPIYDQTYA
                     GGRLGLFVFSQEMVYFSDLKYECRDA"
     exon            443..999
                     /gene="Thbs2"
                     /gene_synonym="Thbs-2; TSP2"
                     /inference="alignment:Splign:2.1.0"
     exon            1000..1084
                     /gene="Thbs2"
                     /gene_synonym="Thbs-2; TSP2"
                     /inference="alignment:Splign:2.1.0"
     exon            1085..1281
                     /gene="Thbs2"
                     /gene_synonym="Thbs-2; TSP2"
                     /inference="alignment:Splign:2.1.0"
     exon            1282..1422
                     /gene="Thbs2"
                     /gene_synonym="Thbs-2; TSP2"
                     /inference="alignment:Splign:2.1.0"
     exon            1423..1519
                     /gene="Thbs2"
                     /gene_synonym="Thbs-2; TSP2"
                     /inference="alignment:Splign:2.1.0"
     exon            1520..1690
                     /gene="Thbs2"
                     /gene_synonym="Thbs-2; TSP2"
                     /inference="alignment:Splign:2.1.0"
     exon            1691..1867
                     /gene="Thbs2"
                     /gene_synonym="Thbs-2; TSP2"
                     /inference="alignment:Splign:2.1.0"
     exon            1868..2041
                     /gene="Thbs2"
                     /gene_synonym="Thbs-2; TSP2"
                     /inference="alignment:Splign:2.1.0"
     exon            2042..2169
                     /gene="Thbs2"
                     /gene_synonym="Thbs-2; TSP2"
                     /inference="alignment:Splign:2.1.0"
     exon            2170..2322
                     /gene="Thbs2"
                     /gene_synonym="Thbs-2; TSP2"
                     /inference="alignment:Splign:2.1.0"
     exon            2323..2541
                     /gene="Thbs2"
                     /gene_synonym="Thbs-2; TSP2"
                     /inference="alignment:Splign:2.1.0"
     exon            2542..2649
                     /gene="Thbs2"
                     /gene_synonym="Thbs-2; TSP2"
                     /inference="alignment:Splign:2.1.0"
     exon            2650..2809
                     /gene="Thbs2"
                     /gene_synonym="Thbs-2; TSP2"
                     /inference="alignment:Splign:2.1.0"
     exon            2810..2928
                     /gene="Thbs2"
                     /gene_synonym="Thbs-2; TSP2"
                     /inference="alignment:Splign:2.1.0"
     exon            2929..3163
                     /gene="Thbs2"
                     /gene_synonym="Thbs-2; TSP2"
                     /inference="alignment:Splign:2.1.0"
     exon            3164..3391
                     /gene="Thbs2"
                     /gene_synonym="Thbs-2; TSP2"
                     /inference="alignment:Splign:2.1.0"
     exon            3392..3663
                     /gene="Thbs2"
                     /gene_synonym="Thbs-2; TSP2"
                     /inference="alignment:Splign:2.1.0"
     exon            3664..3761
                     /gene="Thbs2"
                     /gene_synonym="Thbs-2; TSP2"
                     /inference="alignment:Splign:2.1.0"
     exon            3762..3901
                     /gene="Thbs2"
                     /gene_synonym="Thbs-2; TSP2"
                     /inference="alignment:Splign:2.1.0"
     exon            3902..5922
                     /gene="Thbs2"
                     /gene_synonym="Thbs-2; TSP2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4289..4294
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Thbs2"
                     /gene_synonym="Thbs-2; TSP2"
                     /note="hexamer: CATAAA"
     polyA_site      4310
                     /gene="Thbs2"
                     /gene_synonym="Thbs-2; TSP2"
                     /note="major polyA site"
     regulatory      4947..4952
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Thbs2"
                     /gene_synonym="Thbs-2; TSP2"
                     /note="hexamer: ATTAAA"
     polyA_site      4966
                     /gene="Thbs2"
                     /gene_synonym="Thbs-2; TSP2"
ORIGIN      
        1 gaggggctgg atttggactg ggtaaccagg tactctgagc aaatggcgag accagaggac
       61 gaattatgac actccttcga aagaatgaat taaacgtgcc tccgcatgtg tctgattgac
      121 aggcctcctg gggaaagggc gtataaagga gccggtcact gccgggaggc agagagagcc
      181 agtccgatgt ctgcagcctc cctggccagg cctctcctct cctgccgcag ctagtccccc
      241 tcaggacaga cagagtactg gcgtcggtca ccattcactt gcaaacacac caggtcacgt
      301 gaagaaactt cctggtgaca ctcaggctgt agctgtgcac tcttcaacca cgaggttggt
      361 tttctcctaa gtgtcacagg tggagacaag atgctctggg cactggccct gctggctctg
      421 ggcatagggc caagagcttc tgctggtgac cacgtcaagg acacttcatt tgaccttttc
      481 agcatcagca acattaaccg gaagaccatc ggtgccaagc agttccgagg gcctgacccc
      541 ggggtgcccg cctaccgttt tgtacggttt gactacatcc ccccagtgaa cacagatgat
      601 ctcaacagga ttgtcaagct tgcaaggaga aaggagggct tcttcctcac agcccaactg
      661 aagcaggacc gcaagtctcg gggaacgctc ctggtgttgg aaggccccgg cacctcccag
      721 aggcagtttg agattgtgtc caatggccca ggggacactt tggacctcaa ctactgggta
      781 gaaggcaatc agcataccaa cttcctggag gatgtgggcc tggctgactc ccagtggaag
      841 aatgtgactg tgcaggtggc cagtgacacc tatagcctgt atgtgggctg cgatcttatc
      901 gacagtgtca ccctggaaga accattctat gagcagctag aagtagacag gagcaggatg
      961 tacgtggcca aaggtgcatc tcgagagagt cacttcaggg gcttgctgca gaatgtccat
     1021 ctcgtgtttg cagattctgt ggaagatatc ttaagcaaga aaggctgtca acacagccag
     1081 ggagctgaag tcaacaccat cagtgaacat acagagactc tccatctgag ccctcacatc
     1141 accacagatc tcgtggtcca gggtgtggag aaggcacagg aggtgtgtac gcactcctgc
     1201 gaggagttga gcaacatgat gaatgagctc tctggactgc acgtcatggt gaaccagctg
     1261 agcaagaacc tggagagagt gtctagtgat aaccagttcc ttttggagct cattgggggc
     1321 cctctgaaga caagaaacat gtcagcctgt gtgcaggagg gccgaatctt tgcagaaaat
     1381 gaaacctggg ttgtggatag ttgtaccaca tgcacctgca agaaatttaa aacagtctgc
     1441 catcagatca cctgctcacc tgcaacttgt gccaacccat cttttgtgga aggcgagtgc
     1501 tgtccatcct gttcacactc tgcagacagt gatgagggct ggtctccgtg ggcagagtgg
     1561 accgagtgtt ctgtcacctg tggctctggg acccagcaga gaggccggtc ttgtgatgtc
     1621 accagcaaca cctgcctggg cccctccatt cagacaagga catgcagcct gggcaaatgt
     1681 gatacgagaa tccgtcagaa tggaggctgg agtcactggt caccctggtc ttcatgctcc
     1741 gtgacttgtg gagttggcaa tgtcacccgc atacgtctct gcaactcacc agtgccccag
     1801 atgggtggca agaactgcaa gggcagcggc cgggaaacca aaccctgtca gcgtgatccg
     1861 tgcccaattg atggccgctg gagcccctgg tccccttggt cagcctgcac agttacctgt
     1921 gctggaggga tccgtgagcg ctcacgtgtt tgcaacagcc ctgagcccca gtatggaggg
     1981 aaggactgtg tcggggatgt gacagaacac caaatgtgca acaagagaag ctgccctatt
     2041 gatgggtgct tatccaaccc gtgttttcct ggagccaagt gcaacagctt ccctgatggg
     2101 tcctggtcct gtggctcctg cccagtgggc tttctgggca atggtaccca ctgtgaggac
     2161 ctggatgagt gtgctgtggt cacagatatt tgcttctcaa ctaacaaagc tccccgctgt
     2221 gtcaacacca acccgggctt ccactgcctg ccttgtccac cacgctacaa ggggaaccaa
     2281 cccttcggtg ttggcctgga ggatgctagg acagaaaaac aagtgtgtga gccagagaat
     2341 ccatgtaagg acaagactca cagctgccac aagaatgcag agtgcatcta cctgggccac
     2401 tttagtgacc ccatgtacaa gtgtgagtgc cagattggct acgcaggtga tgggctcatc
     2461 tgcggggagg actcagacct ggatggctgg cccaacaaca acctggtgtg tgctactaat
     2521 gccacctacc actgcatcaa ggacaactgc cccaaactgc caaattccgg gcaggaggat
     2581 tttgataagg atggaatcgg agatgcttgt gacgaggacg atgacaatga cggtgtgagc
     2641 gatgagaagg acaattgcca gcttctcttc aatccccgtc aattagacta tgacaaggat
     2701 gaggttggag accgctgtga caactgcccc tatgtgcaca acccagcaca gatcgacaca
     2761 gacaacaatg gcgaggggga tgcctgctct gtggacattg acggagacga tgttttcaat
     2821 gagcgagaca attgtccata tgtctacaac actgaccaga gagatacgga tggtgatggc
     2881 gtgggtgacc actgtgacaa ttgtcctctg atgcacaacc cagatcagat cgatcaggac
     2941 aatgatctcg ttggagacca gtgtgacaac aatgaggaca tagatgatga cggccaccag
     3001 aacaaccaag acaactgccc atacatctcc aactccaacc aggctgacca tgacaacgac
     3061 ggcaagggcg atgcctgcga ctctgatgat gacaatgatg gtgttccaga tgacagggac
     3121 aactgtcggc ttgtgttcaa cccagaccag gaagactcgg acggtgacgg ccgaggtgac
     3181 atttgtaaag atgactttga caatgataat gtcccagata ttgatgatgt gtgccctgag
     3241 aacaatgcca tcactgagac agacttcaga aacttccaga tggtccctct ggatcccaag
     3301 gggaccacac aaattgatcc caactgggta attcgtcacc aaggcaaaga gctggtgcag
     3361 acagcaaact cagaccctgg catcgctgta ggtttcgacg agtttgggtc tgtggacttc
     3421 agtggcactt tctatgtcaa cactgaccgg gatgatgact acgctggctt tgtctttggc
     3481 tatcagtcaa gcagccgctt ctatgtggtg atgtggaagc aggtgaccca gacctactgg
     3541 gaagacaagc ccagtcgggc ttacggctac tctggtgtgt cactcaaagt ggtaaactcc
     3601 acgactggta ctggcgagca cctgaggaat gccctgtggc acacgggaaa cacagaaggc
     3661 caggtccgga ctctatggca tgaccccaaa aacattggct ggaaagacta cactgcctac
     3721 aggtggcacc tgattcacag gcctaagaca ggctacatga gagtcttagt gcatgaagga
     3781 aagcaagtca tggctgactc aggaccaatt tatgaccaaa cctacgctgg tggacggctg
     3841 ggcctgtttg tcttctccca agagatggtc tatttctcgg acctcaagta tgagtgcaga
     3901 gatgcctaga gagcagggct ccagctccag caatgtgctg caaacacccc ttcttagaca
     3961 catcagtcca tcttggcact tgtggctttt ctgtcatttg gcatttcctg tttcttgacc
     4021 ttaactgagt ggatctacac ctccttcatc agcaccaagt ccaagtgtct tcaaaggaga
     4081 aacatcaatt gcactccaag agcttccagc ctgctgctgg aaaacatttg gatgagatat
     4141 gaggctcacc gtggagcgaa gaccgagcat tccgctgtgt tgccttttct tgtttgttta
     4201 aaaagaatga cgtttacatg taaatgtaat tacttgcagt atttatgtgt atatggagtc
     4261 gaagggagta ttgtgtttaa gtcattctca taaattaagc atgaaaaata tggctcactt
     4321 accctcagtg cttaatgttg tcactgtttt tggattcgtg ctgttctata atgacacaca
     4381 aatcctgtga ggctgaacag gaagaaagat caggtgaact cggctgcagt aaggaagagg
     4441 ccagaagaca gtcatgtgag actgtggccc cttgtccagt gatttgaaac attaactaat
     4501 tagaacatag gaagtgatcg aaaagttaga agaaacgcag ttcttacttg gccacagtta
     4561 aagctgactc gacagagaca aagaagctgc atcctttgaa aggggaatat ttcagggttc
     4621 cagctgtagg cttctcagag ctttcagaag aaaacctcag cagaggcaat gtccatttca
     4681 gctgcctgcc tgggttgggc agtgtggaaa gcatacctgg ctggacgtca tcaggctctg
     4741 catactctgc atactgactg ctaggatctt tgtctgggca tcctttcctg tccctgctgc
     4801 taggaaacag actggcaaat aagttggcag atgcactttg gtaccctgtt cctactctag
     4861 gctttagaat gatgcaaaag ctgcttttgc caaatttggt aatgcatttc cttaaggttc
     4921 caggtacggc tcttttgtta atatttatta aatgatttgg aatatagctt cactcatcaa
     4981 taatttattt taaacataca aagtaacaag tatgtagtat ataatttcta gaaataagca
     5041 tatgacaagg atataatgat ataaagacac acttacaaat atctgctgtt tgaaatacaa
     5101 ggatttatgt catgatagaa tggaacctta atatgcatcc ttaccaataa ttttaattca
     5161 gttcttttta ctattatctc tttgttgtat ataggttctt tttattttaa tgcttaagga
     5221 aacatcattt gttttttcca gacacaccca ttaatcagtc acatcattac agacatatgc
     5281 ttgatttatt ttattttgtt tttgttttgc tttaagctgc atgatctccc agcaggaagt
     5341 actgcctctc tcacttcaca cgagctcagt ggaagaatat tttgtccctc tggtggggta
     5401 gttcacagtc ccctttctag taatggcaac tgcatttcta agttagacag aaagaatcca
     5461 cgattttgat ctgagtttcc acaatttgtc tagatggagt tatttgtgtt gaaatctctg
     5521 agggtgagtt tccttgtttc ttgttatcct gcacttttta gatttttgtg gaactgtatt
     5581 cccaaggcca gcacaggtca ggagacttct ggccatgagg gtgtgaagaa catttaggct
     5641 ctcacttggg catttggttc aatggtacaa ggtggtggga gcgatgtgaa tacattcaat
     5701 gtaccatatt atttttgtaa attatttatg tagttttttt taaaaacaga agcttctcat
     5761 atacgttaat gaaacattgt tttttttttt ttgccaaaga ttgtaaatat ttatttattt
     5821 gttcattggg ttaaatttca ccactaaaac ccctcattta gctagagctt catttttata
     5881 ccaaagatta tcaacagcaa ataaattata aaaaggtttc tg
//
