LOCUS       NP_035711               1172 aa            linear   ROD 27-APR-2025
DEFINITION  thrombospondin-2 precursor [Mus musculus].
ACCESSION   NP_035711
VERSION     NP_035711.2
DBSOURCE    REFSEQ: accession NM_011581.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (residues 1 to 1172)
  AUTHORS   Shu,H., Parada,I., Delgado,A., Prince,D.A. and Gu,F.
  TITLE     Increased excitatory connectivity and epileptiform activity in
            thrombospondin1/2 knockout mice following cortical trauma
  JOURNAL   Neurobiol Dis 200, 106634 (2024)
   PUBMED   39122122
  REMARK    GeneRIF: Increased excitatory connectivity and epileptiform
            activity in thrombospondin1/2 knockout mice following cortical
            trauma.
REFERENCE   2  (residues 1 to 1172)
  AUTHORS   Hadar,N., Porgador,O., Cohen,I., Levi,H., Dolgin,V., Yogev,Y.,
            Sued-Hendrickson,S., Shelef,I., Didkovsky,E., Eskin-Schwartz,M. and
            Birk,O.S.
  TITLE     Heterozygous THBS2 pathogenic variant causes Ehlers-Danlos syndrome
            with prominent vascular features in humans and mice
  JOURNAL   Eur J Hum Genet 32 (5), 550-557 (2024)
   PUBMED   38433265
  REMARK    GeneRIF: Heterozygous THBS2 pathogenic variant causes Ehlers-Danlos
            syndrome with prominent vascular features in humans and mice.
REFERENCE   3  (residues 1 to 1172)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   4  (residues 1 to 1172)
  AUTHORS   Chen,Z., Huang,Y., Xing,H., Tseng,T., Edelman,H., Perry,R. and
            Kyriakides,T.R.
  TITLE     Novel muscle-derived extracellular matrix hydrogel promotes
            angiogenesis and neurogenesis in volumetric muscle loss
  JOURNAL   Matrix Biol 127, 38-47 (2024)
   PUBMED   38325441
REFERENCE   5  (residues 1 to 1172)
  AUTHORS   Li,X., Ding,Y., Zhou,M., Zhou,Z., Xie,T., Wang,Y., Liu,Z., Si,Y.
            and Shi,Z.
  TITLE     Deficiency of thrombospondin-2 alleviates intimal hyperplasia in
            mice by modulating vascular smooth muscle cell proliferation and
            migration
  JOURNAL   FASEB J 37 (2), e22743 (2023)
   PUBMED   36645109
  REMARK    GeneRIF: Deficiency of thrombospondin-2 alleviates intimal
            hyperplasia in mice by modulating vascular smooth muscle cell
            proliferation and migration.
REFERENCE   6  (residues 1 to 1172)
  AUTHORS   Himmelbauer,H., Harvey,R.P., Copeland,N.G., Jenkins,N.A. and
            Silver,L.M.
  TITLE     High-resolution genetic analysis of a deletion on mouse chromosome
            17 extending over the fused, tufted, and homeobox Nkx2-5 loci
  JOURNAL   Mamm Genome 5 (12), 814-816 (1994)
   PUBMED   7894168
REFERENCE   7  (residues 1 to 1172)
  AUTHORS   Trachtulec,Z., Vincek,V., Hamvas,R.M., Forejt,J., Lehrach,H. and
            Klein,J.
  TITLE     Physical map of mouse chromosome 17 in the region relevant for
            positional cloning of the Hybrid sterility 1 gene
  JOURNAL   Genomics 23 (1), 132-137 (1994)
   PUBMED   7829061
REFERENCE   8  (residues 1 to 1172)
  AUTHORS   Laherty,C.D., O'Rourke,K., Wolf,F.W., Katz,R., Seldin,M.F. and
            Dixit,V.M.
  TITLE     Characterization of mouse thrombospondin 2 sequence and expression
            during cell growth and development
  JOURNAL   J Biol Chem 267 (5), 3274-3281 (1992)
   PUBMED   1371115
REFERENCE   9  (residues 1 to 1172)
  AUTHORS   Bornstein,P., Devarayalu,S., Li,P., Disteche,C.M. and Framson,P.
  TITLE     A second thrombospondin gene in the mouse is similar in
            organization to thrombospondin 1 but does not respond to serum
  JOURNAL   Proc Natl Acad Sci U S A 88 (19), 8636-8640 (1991)
   PUBMED   1924325
REFERENCE   10 (residues 1 to 1172)
  AUTHORS   Bornstein,P., O'Rourke,K., Wikstrom,K., Wolf,F.W., Katz,R., Li,P.
            and Dixit,V.M.
  TITLE     A second, expressed thrombospondin gene (Thbs2) exists in the mouse
            genome
  JOURNAL   J Biol Chem 266 (20), 12821-12824 (1991)
   PUBMED   1712771
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            CJ049934.1, AK132508.1, AK142552.1 and AK165881.1.
            
            On Jun 11, 2009 this sequence version replaced NP_035711.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK142552.1, L07803.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..1172
                     /organism="Mus musculus"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="17"
                     /map="17 8.95 cM"
     Protein         1..1172
                     /product="thrombospondin-2 precursor"
                     /note="Thrombospondin-2"
                     /calculated_mol_wt=128076
     sig_peptide     1..18
                     /inference="COORDINATES: ab initio prediction:SignalP:6.0"
                     /calculated_mol_wt=1824
     mat_peptide     19..1172
                     /product="Thrombospondin-2. /id=PRO_0000035847"
                     /note="propagated from UniProtKB/Swiss-Prot (Q03350.2)"
                     /calculated_mol_wt=128076
     Region          19..232
                     /region_name="Heparin-binding. /evidence=ECO:0000255"
                     /note="propagated from UniProtKB/Swiss-Prot (Q03350.2)"
     Region          21..213
                     /region_name="TSPN"
                     /note="Thrombospondin N-terminal -like domains;
                     smart00210"
                     /db_xref="CDD:214560"
     Site            151
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (Q03350.2)"
     Site            316
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (Q03350.2)"
     Region          320..374
                     /region_name="VWC"
                     /note="von Willebrand factor type C domain; pfam00093"
                     /db_xref="CDD:278520"
     Site            330
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (Q03350.2)"
     Region          385..430
                     /region_name="TSP_1"
                     /note="Thrombospondin type 1 domain; pfam00090"
                     /db_xref="CDD:459668"
     Region          440..492
                     /region_name="TSP1"
                     /note="Thrombospondin type 1 repeats; smart00209"
                     /db_xref="CDD:214559"
     Site            457
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (Q03350.2)"
     Region          497..549
                     /region_name="TSP1"
                     /note="Thrombospondin type 1 repeats; smart00209"
                     /db_xref="CDD:214559"
     Site            584
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (Q03350.2)"
     Region          652..691
                     /region_name="EGF_3"
                     /note="EGF domain; pfam12947"
                     /db_xref="CDD:463759"
     Region          693..728
                     /region_name="TSP type-3 1"
                     /note="propagated from UniProtKB/Swiss-Prot (Q03350.2)"
     Region          693..728
                     /region_name="TSP3 repeat_1C"
                     /note="TSP3 repeat_1C [structural motif]"
                     /db_xref="CDD:275367"
     Site            710
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (Q03350.2)"
     Region          727..752
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (Q03350.2)"
     Region          729..764
                     /region_name="TSP type-3 2"
                     /note="propagated from UniProtKB/Swiss-Prot (Q03350.2)"
     Region          729..764
                     /region_name="TSP3 repeat_long"
                     /note="TSP3 repeat_long [structural motif]"
                     /db_xref="CDD:275366"
     Region          765..787
                     /region_name="TSP type-3 3"
                     /note="propagated from UniProtKB/Swiss-Prot (Q03350.2)"
     Region          765..787
                     /region_name="TSP3 repeat_short"
                     /note="TSP3 repeat_short [structural motif]"
                     /db_xref="CDD:275365"
     Region          788..823
                     /region_name="TSP type-3 4"
                     /note="propagated from UniProtKB/Swiss-Prot (Q03350.2)"
     Region          789..823
                     /region_name="TSP_3"
                     /note="Thrombospondin type 3 repeat; pfam02412"
                     /db_xref="CDD:367074"
     Region          789..823
                     /region_name="TSP3 repeat_long"
                     /note="TSP3 repeat_long [structural motif]"
                     /db_xref="CDD:275366"
     Region          824..846
                     /region_name="TSP type-3 5"
                     /note="propagated from UniProtKB/Swiss-Prot (Q03350.2)"
     Region          824..846
                     /region_name="TSP3 repeat_short"
                     /note="TSP3 repeat_short [structural motif]"
                     /db_xref="CDD:275366"
     Region          846..938
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (Q03350.2)"
     Region          847..884
                     /region_name="TSP3 repeat_long"
                     /note="TSP3 repeat_long [structural motif]"
                     /db_xref="CDD:275365"
     Region          848..884
                     /region_name="TSP_3"
                     /note="Thrombospondin type 3 repeat; pfam02412"
                     /db_xref="CDD:367074"
     Region          885..920
                     /region_name="TSP3 repeat_short"
                     /note="TSP3 repeat_short [structural motif]"
                     /db_xref="CDD:275366"
     Region          921..956
                     /region_name="TSP type-3 8"
                     /note="propagated from UniProtKB/Swiss-Prot (Q03350.2)"
     Region          921..951
                     /region_name="TSP_3"
                     /note="Thrombospondin type 3 repeat; pfam02412"
                     /db_xref="CDD:367074"
     Region          928..930
                     /region_name="Cell attachment site. /evidence=ECO:0000255"
                     /note="propagated from UniProtKB/Swiss-Prot (Q03350.2)"
     Region          974..1171
                     /region_name="TSP_C"
                     /note="Thrombospondin C-terminal region; pfam05735"
                     /db_xref="CDD:461725"
     Site            1069
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (Q03350.2)"
     CDS             1..1172
                     /gene="Thbs2"
                     /gene_synonym="Thbs-2; TSP2"
                     /coded_by="NM_011581.3:391..3909"
                     /db_xref="CCDS:CCDS49958.1"
                     /db_xref="GeneID:21826"
                     /db_xref="MGI:MGI:98738"
ORIGIN      
        1 mlwalallal gigprasagd hvkdtsfdlf sisninrkti gakqfrgpdp gvpayrfvrf
       61 dyippvntdd lnrivklarr kegffltaql kqdrksrgtl lvlegpgtsq rqfeivsngp
      121 gdtldlnywv egnqhtnfle dvgladsqwk nvtvqvasdt yslyvgcdli dsvtleepfy
      181 eqlevdrsrm yvakgasres hfrgllqnvh lvfadsvedi lskkgcqhsq gaevntiseh
      241 tetlhlsphi ttdlvvqgve kaqevcthsc eelsnmmnel sglhvmvnql sknlervssd
      301 nqflleligg plktrnmsac vqegrifaen etwvvdsctt ctckkfktvc hqitcspatc
      361 anpsfvegec cpscshsads degwspwaew tecsvtcgsg tqqrgrscdv tsntclgpsi
      421 qtrtcslgkc dtrirqnggw shwspwsscs vtcgvgnvtr irlcnspvpq mggknckgsg
      481 retkpcqrdp cpidgrwspw spwsactvtc aggirersrv cnspepqygg kdcvgdvteh
      541 qmcnkrscpi dgclsnpcfp gakcnsfpdg swscgscpvg flgngthced ldecavvtdi
      601 cfstnkaprc vntnpgfhcl pcpprykgnq pfgvgledar tekqvcepen pckdkthsch
      661 knaeciylgh fsdpmykcec qigyagdgli cgedsdldgw pnnnlvcatn atyhcikdnc
      721 pklpnsgqed fdkdgigdac dedddndgvs dekdncqllf nprqldydkd evgdrcdncp
      781 yvhnpaqidt dnngegdacs vdidgddvfn erdncpyvyn tdqrdtdgdg vgdhcdncpl
      841 mhnpdqidqd ndlvgdqcdn nedidddghq nnqdncpyis nsnqadhdnd gkgdacdsdd
      901 dndgvpddrd ncrlvfnpdq edsdgdgrgd ickddfdndn vpdiddvcpe nnaitetdfr
      961 nfqmvpldpk gttqidpnwv irhqgkelvq tansdpgiav gfdefgsvdf sgtfyvntdr
     1021 dddyagfvfg yqsssrfyvv mwkqvtqtyw edkpsraygy sgvslkvvns ttgtgehlrn
     1081 alwhtgnteg qvrtlwhdpk nigwkdytay rwhlihrpkt gymrvlvheg kqvmadsgpi
     1141 ydqtyaggrl glfvfsqemv yfsdlkyecr da
//
