LOCUS       NM_001313914            5862 bp    mRNA    linear   ROD 04-MAR-2025
DEFINITION  Mus musculus thrombospondin 1 (Thbs1), transcript variant 2, mRNA.
ACCESSION   NM_001313914 XM_006499152
VERSION     NM_001313914.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 5862)
  AUTHORS   Shigesawa,I., Nakamura,A., Yamauchi,Y., Kawata,S., Miyazaki,A.,
            Nomoto,H., Kameda,H., Terauchi,Y. and Atsumi,T.
  TITLE     Effects of glucokinase haploinsufficiency on the pancreatic
            beta-cell mass and function of long-term high-fat, high-sucrose
            diet-fed mice
  JOURNAL   J Diabetes Investig 15 (12), 1732-1742 (2024)
   PUBMED   39305123
REFERENCE   2  (bases 1 to 5862)
  AUTHORS   Shu,H., Parada,I., Delgado,A., Prince,D.A. and Gu,F.
  TITLE     Increased excitatory connectivity and epileptiform activity in
            thrombospondin1/2 knockout mice following cortical trauma
  JOURNAL   Neurobiol Dis 200, 106634 (2024)
   PUBMED   39122122
  REMARK    GeneRIF: Increased excitatory connectivity and epileptiform
            activity in thrombospondin1/2 knockout mice following cortical
            trauma.
REFERENCE   3  (bases 1 to 5862)
  AUTHORS   Patel,V.N., Aure,M.H., Choi,S.H., Ball,J.R., Lane,E.D., Wang,Z.,
            Xu,Y., Zheng,C., Liu,X., Martin,D., Pailin,J.Y., Prochazkova,M.,
            Kulkarni,A.B., van Kuppevelt,T.H., Ambudkar,I.S., Liu,J. and
            Hoffman,M.P.
  TITLE     Specific 3-O-sulfated heparan sulfate domains regulate salivary
            gland basement membrane metabolism and epithelial differentiation
  JOURNAL   Nat Commun 15 (1), 7584 (2024)
   PUBMED   39217171
  REMARK    Erratum:[Nat Commun. 2024 Oct 10;15(1):8784. doi:
            10.1038/s41467-024-53230-4. PMID: 39389981]
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 5862)
  AUTHORS   Fan,Y., Dong,S., Xia,Y., Yang,X., Lei,Q., Xu,F., Liang,D.,
            Liang,S., Zhang,M., Yang,F., Jing,Y., Li,L., Zhu,X., Bao,H.,
            Chen,Z. and Zeng,C.
  TITLE     Role of TSP-1 and its receptor ITGB3 in the renal
            tubulointerstitial injury of focal segmental glomerulosclerosis
  JOURNAL   J Biol Chem 300 (8), 107516 (2024)
   PUBMED   38960036
  REMARK    GeneRIF: Role of TSP-1 and its receptor ITGB3 in the renal
            tubulointerstitial injury of focal segmental glomerulosclerosis.
REFERENCE   5  (bases 1 to 5862)
  AUTHORS   Wilson,Z.S., Raya-Sandino,A., Miranda,J., Fan,S., Brazil,J.C.,
            Quiros,M., Garcia-Hernandez,V., Liu,Q., Kim,C.H., Hankenson,K.D.,
            Nusrat,A. and Parkos,C.A.
  TITLE     Critical role of thrombospondin-1 in promoting intestinal mucosal
            wound repair
  JOURNAL   JCI Insight 9 (17), e180608 (2024)
   PUBMED   39078701
  REMARK    GeneRIF: Critical role of thrombospondin-1 in promoting intestinal
            mucosal wound repair.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 5862)
  AUTHORS   Rochelle,J.M., Watson,M.L., Oakey,R.J. and Seldin,M.F.
  TITLE     A linkage map of mouse chromosome 19: definition of comparative
            mapping relationships with human chromosomes 10 and 11 including
            the MEN1 locus
  JOURNAL   Genomics 14 (1), 26-31 (1992)
   PUBMED   1358795
REFERENCE   7  (bases 1 to 5862)
  AUTHORS   Laherty,C.D., O'Rourke,K., Wolf,F.W., Katz,R., Seldin,M.F. and
            Dixit,V.M.
  TITLE     Characterization of mouse thrombospondin 2 sequence and expression
            during cell growth and development
  JOURNAL   J Biol Chem 267 (5), 3274-3281 (1992)
   PUBMED   1371115
REFERENCE   8  (bases 1 to 5862)
  AUTHORS   Lawler,J., Duquette,M., Ferro,P., Copeland,N.G., Gilbert,D.J. and
            Jenkins,N.A.
  TITLE     Characterization of the murine thrombospondin gene
  JOURNAL   Genomics 11 (3), 587-600 (1991)
   PUBMED   1774063
REFERENCE   9  (bases 1 to 5862)
  AUTHORS   Bornstein,P., O'Rourke,K., Wikstrom,K., Wolf,F.W., Katz,R., Li,P.
            and Dixit,V.M.
  TITLE     A second, expressed thrombospondin gene (Thbs2) exists in the mouse
            genome
  JOURNAL   J Biol Chem 266 (20), 12821-12824 (1991)
   PUBMED   1712771
REFERENCE   10 (bases 1 to 5862)
  AUTHORS   Jaffe,E., Bornstein,P. and Disteche,C.M.
  TITLE     Mapping of the thrombospondin gene to human chromosome 15 and mouse
            chromosome 2 by in situ hybridization
  JOURNAL   Genomics 7 (1), 123-126 (1990)
   PUBMED   2335352
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL928957.9, AK163092.1,
            BC050917.1 and AL845495.9.
            
            On Sep 11, 2015 this sequence version replaced XM_006499152.1.
            
            Summary: The protein encoded by this gene is a subunit of a
            disulfide-linked homotrimeric protein. This protein is an adhesive
            glycoprotein that mediates cell-to-cell and cell-to-matrix
            interactions. This protein can bind to fibrinogen, fibronectin,
            laminin, type V collagen and integrins alpha-V/beta-1. This protein
            has been shown to play roles in platelet aggregation, angiogenesis,
            and tumorigenesis. Alternative splicing results in multiple
            transcript variants encoding different isoforms. [provided by
            RefSeq, Sep 2015].
            
            Transcript Variant: This variant (2) uses an alternate in-frame
            splice site in the 5' coding region, compared to variant 1. The
            encoded isoform (2) is shorter than isoform 1.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK163092.1, M87276.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-17                AL928957.9         96536-96552
            18-225              AK163092.1         2-209
            226-226             AL928957.9         97300-97300
            227-3798            AK163092.1         211-3782
            3799-3799           AL928957.9         109747-109747
            3800-4115           AK163092.1         3784-4099
            4116-4487           BC050917.1         4073-4444
            4488-4737           AL928957.9         110436-110685
            4738-5593           AL845495.9         2001-2856
            5594-5862           BC050917.1         5551-5819
FEATURES             Location/Qualifiers
     source          1..5862
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 59.34 cM"
     gene            1..5862
                     /gene="Thbs1"
                     /gene_synonym="tbsp1; Thbs-1; TSP-1; TSP1"
                     /note="thrombospondin 1"
                     /db_xref="GeneID:21825"
                     /db_xref="MGI:MGI:98737"
     exon            1..200
                     /gene="Thbs1"
                     /gene_synonym="tbsp1; Thbs-1; TSP-1; TSP1"
                     /inference="alignment:Splign:2.1.0"
     exon            201..295
                     /gene="Thbs1"
                     /gene_synonym="tbsp1; Thbs-1; TSP-1; TSP1"
                     /inference="alignment:Splign:2.1.0"
     CDS             229..3741
                     /gene="Thbs1"
                     /gene_synonym="tbsp1; Thbs-1; TSP-1; TSP1"
                     /note="isoform 2 precursor is encoded by transcript
                     variant 2; glycoprotein G"
                     /codon_start=1
                     /product="thrombospondin-1 isoform 2 precursor"
                     /protein_id="NP_001300843.1"
                     /db_xref="GeneID:21825"
                     /db_xref="MGI:MGI:98737"
                     /translation="MELLRGLGVLFLLHMCGSNRIPESGGDNGVFDIFELIGGARRGP
                     GRRLVKGQDLSSPAFRIENANLIPAVPDDKFQDLLDAVWADKGFIFLASLRQMKKTRG
                     TLLAVERKDNTGQIFSVVSNGKAGTLDLSLSLPGKQQVVSVEEALLATGQWKSITLFV
                     QEDRAQLYIDCDKMESAELDVPIQSIFTRDLASVARLRVAKGDVNDNFQGVLQNVRFV
                     FGTTPEDILRNKGCSSSTNVLLTLDNNVVNGSSPAIRTNYIGHKTKDLQAICGLSCDE
                     LSSMVLELKGLRTIVTTLQDSIRKVTEENRELVSELKRPPLCFHNGVQYKNNEEWTVD
                     SCTECHCQNSVTICKKVSCPIMPCSNATVPDGECCPRCWPSDSADDGWSPWSEWTSCS
                     ATCGNGIQQRGRSCDSLNNRCEGSSVQTRTCHIQECDKRFKQDGGWSHWSPWSSCSVT
                     CGDGVITRIRLCNSPSPQMNGKPCEGEARETKACKKDACPINGGWGPWSPWDICSVTC
                     GGGVQRRSRLCNNPTPQFGGKDCVGDVTENQVCNKQDCPIDGCLSNPCFAGAKCTSYP
                     DGSWKCGACPPGYSGNGIQCKDVDECKEVPDACFNHNGEHRCKNTDPGYNCLPCPPRF
                     TGSQPFGRGVEHAMANKQVCKPRNPCTDGTHDCNKNAKCNYLGHYSDPMYRCECKPGY
                     AGNGIICGEDTDLDGWPNENLVCVANATYHCKKDNCPNLPNSGQEDYDKDGIGDACDD
                     DDDNDKIPDDRDNCPFHYNPAQYDYDRDDVGDRCDNCPYNHNPDQADTDKNGEGDACA
                     VDIDGDGILNERDNCQYVYNVDQRDTDMDGVGDQCDNCPLEHNPDQLDSDSDLIGDTC
                     DNNQDIDEDGHQNNLDNCPYVPNANQADHDKDGKGDACDHDDDNDGIPDDRDNCRLVP
                     NPDQKDSDGDGRGDACKDDFDHDNVPDIDDICPENFDISETDFRQFQMIPLDPKGTSQ
                     NDPNWVVRHQGKELVQTVNCDPGLAVGYDEFNAVDFSGTFFINTERDDDYAGFVFGYQ
                     SSSRFYVVMWKQVTQSYWDTNPTRAQGYSGLSVKVVNSTTGPGEHLRNALWHTGNTPG
                     QVRTLWHDPRHIGWKDFTAYRWRLSHRPKTGYIRVVMYEGKKIMADSGPIYDKTYAGG
                     RLGLFVFSQEMVFFSDMKYECRDS"
     exon            296..855
                     /gene="Thbs1"
                     /gene_synonym="tbsp1; Thbs-1; TSP-1; TSP1"
                     /inference="alignment:Splign:2.1.0"
     exon            856..931
                     /gene="Thbs1"
                     /gene_synonym="tbsp1; Thbs-1; TSP-1; TSP1"
                     /inference="alignment:Splign:2.1.0"
     exon            932..1131
                     /gene="Thbs1"
                     /gene_synonym="tbsp1; Thbs-1; TSP-1; TSP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1132..1254
                     /gene="Thbs1"
                     /gene_synonym="tbsp1; Thbs-1; TSP-1; TSP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1255..1348
                     /gene="Thbs1"
                     /gene_synonym="tbsp1; Thbs-1; TSP-1; TSP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1349..1522
                     /gene="Thbs1"
                     /gene_synonym="tbsp1; Thbs-1; TSP-1; TSP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1523..1699
                     /gene="Thbs1"
                     /gene_synonym="tbsp1; Thbs-1; TSP-1; TSP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1700..1873
                     /gene="Thbs1"
                     /gene_synonym="tbsp1; Thbs-1; TSP-1; TSP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1874..2001
                     /gene="Thbs1"
                     /gene_synonym="tbsp1; Thbs-1; TSP-1; TSP1"
                     /inference="alignment:Splign:2.1.0"
     exon            2002..2154
                     /gene="Thbs1"
                     /gene_synonym="tbsp1; Thbs-1; TSP-1; TSP1"
                     /inference="alignment:Splign:2.1.0"
     exon            2155..2373
                     /gene="Thbs1"
                     /gene_synonym="tbsp1; Thbs-1; TSP-1; TSP1"
                     /inference="alignment:Splign:2.1.0"
     exon            2374..2481
                     /gene="Thbs1"
                     /gene_synonym="tbsp1; Thbs-1; TSP-1; TSP1"
                     /inference="alignment:Splign:2.1.0"
     exon            2482..2641
                     /gene="Thbs1"
                     /gene_synonym="tbsp1; Thbs-1; TSP-1; TSP1"
                     /inference="alignment:Splign:2.1.0"
     exon            2642..2760
                     /gene="Thbs1"
                     /gene_synonym="tbsp1; Thbs-1; TSP-1; TSP1"
                     /inference="alignment:Splign:2.1.0"
     exon            2761..2995
                     /gene="Thbs1"
                     /gene_synonym="tbsp1; Thbs-1; TSP-1; TSP1"
                     /inference="alignment:Splign:2.1.0"
     exon            2996..3223
                     /gene="Thbs1"
                     /gene_synonym="tbsp1; Thbs-1; TSP-1; TSP1"
                     /inference="alignment:Splign:2.1.0"
     exon            3224..3495
                     /gene="Thbs1"
                     /gene_synonym="tbsp1; Thbs-1; TSP-1; TSP1"
                     /inference="alignment:Splign:2.1.0"
     exon            3496..3593
                     /gene="Thbs1"
                     /gene_synonym="tbsp1; Thbs-1; TSP-1; TSP1"
                     /inference="alignment:Splign:2.1.0"
     exon            3594..3733
                     /gene="Thbs1"
                     /gene_synonym="tbsp1; Thbs-1; TSP-1; TSP1"
                     /inference="alignment:Splign:2.1.0"
     exon            3734..5845
                     /gene="Thbs1"
                     /gene_synonym="tbsp1; Thbs-1; TSP-1; TSP1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5362..5367
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Thbs1"
                     /gene_synonym="tbsp1; Thbs-1; TSP-1; TSP1"
                     /note="hexamer: CATAAA"
     polyA_site      5392
                     /gene="Thbs1"
                     /gene_synonym="tbsp1; Thbs-1; TSP-1; TSP1"
                     /note="major polyA site"
     regulatory      5817..5822
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Thbs1"
                     /gene_synonym="tbsp1; Thbs-1; TSP-1; TSP1"
                     /note="hexamer: AATAAA"
     polyA_site      5845
                     /gene="Thbs1"
                     /gene_synonym="tbsp1; Thbs-1; TSP-1; TSP1"
                     /note="major polyA site"
ORIGIN      
        1 ggctcctcag tcaagccagc cactgcctgg agtcagccag cctcatcgga cttctgcagg
       61 caatcgcgaa gctgctatcc agttctgcca cggtctctcc cggcgcaccg gcagtctcag
      121 cgtcttcacc ggactcagcg tccttgtcct tcacttcacc tttgccacct ctccgggtta
      181 ctgagccccg gtgcacacag gctccgtgtt gggcacaaag gctccaccat ggagctcctg
      241 cggggactag gtgtcctgtt cctgttgcat atgtgtggaa gcaaccgcat tccagagtct
      301 gggggagata acggtgtgtt tgacatcttt gaactcattg gaggtgcacg aaggggcccc
      361 ggtcgccgac tggtgaaggg ccaagatcta tccagccccg ccttccgcat tgagaatgcc
      421 aacctgatcc ccgctgtgcc ggatgacaag ttccaagacc tactggacgc tgtgtgggcc
      481 gacaaaggct tcatcttcct ggcttccttg aggcagatga agaagacccg gggcacactc
      541 ctggctgtgg aacggaaaga caacactggc cagatcttca gtgtggtctc caacggcaaa
      601 gctggcaccc tggacctgag cctgagcctg ccagggaagc aacaagtggt gtcagtggag
      661 gaagctctcc tggccactgg ccagtggaag agcatcacgc tgtttgttca agaggaccgg
      721 gctcaactct acatagactg tgataagatg gagagcgcgg agctggatgt acccatccag
      781 agcatcttca ccagggatct ggccagcgtt gccaggctcc gagttgcaaa gggagatgtc
      841 aatgacaatt ttcagggggt gctgcagaat gtgaggtttg tctttggaac caccccagaa
      901 gacattctca ggaacaaagg ctgctccagc tctaccaacg tccttcttac ccttgacaac
      961 aacgtggtga acggttccag ccctgctatc cgcaccaact acatcggcca caaaacaaag
     1021 gacctccaag ctatctgtgg cctctcctgt gatgaactat ccagcatggt cctggaactg
     1081 aagggcctgc gcaccatcgt gaccactctg caggacagca tccgaaaagt gacggaagag
     1141 aacagagagc tggtcagtga gctgaagcgg cctcccctct gctttcacaa tggagtccag
     1201 tacaagaaca acgaggagtg gactgtagac agttgcacag agtgtcactg ccagaactcg
     1261 gttaccatct gcaaaaaggt gtcctgtccc atcatgccct gctccaacgc cacagttcct
     1321 gatggtgaat gctgcccacg gtgctggccc agcgactctg ctgacgatgg ctggtctccc
     1381 tggtctgagt ggacctcctg ctctgccaca tgtggcaatg gaattcagca acgtggtcgt
     1441 tcctgtgaca gcctcaacaa cagatgcgag ggctcttcgg tacagacaag gacctgccac
     1501 attcaggagt gtgacaaaag atttaaacag gatggtggct ggagtcactg gtctccatgg
     1561 tcgtcctgtt ctgtgacctg tggtgacggt gtgatcacaa ggatccggct ctgcaactcc
     1621 cccagccccc agatgaacgg gaagccctgt gaaggtgaag cccgggagac caaagcctgc
     1681 aagaaagacg cctgcccaat taatggaggc tggggtccct ggtcaccatg ggacatctgc
     1741 tctgtcacct gtggaggagg agtgcagaga cgcagccgac tctgtaacaa ccccacaccc
     1801 cagtttggag gcaaagactg tgttggcgat gtgacagaaa atcaagtttg caacaagcag
     1861 gactgcccaa ttgatggatg cctgtccaat ccctgctttg ctggtgccaa gtgtactagc
     1921 taccctgatg gtagctggaa atgtggtgcg tgtcctcctg gctacagtgg aaatggcatc
     1981 cagtgcaaag acgtcgatga gtgcaaagaa gtgcctgatg cttgcttcaa tcacaacgga
     2041 gaacatcggt gcaagaacac agatcctggc tacaactgcc tgccctgccc accacgattc
     2101 actggctcac agcccttcgg ccgaggtgtc gaacatgcca tggccaacaa acaggtgtgc
     2161 aaaccgcgaa acccctgcac ggacgggacg catgactgca acaagaacgc taagtgcaac
     2221 tacctgggtc actacagtga ccccatgtac cgctgtgagt gcaagcccgg ctatgcaggc
     2281 aatggcatca tctgcggaga ggacacagac ctggacggct ggcctaatga aaacctggtg
     2341 tgtgtggcca acgcaaccta ccactgcaaa aaggacaact gccccaacct tcccaactcg
     2401 gggcaggaag actatgacaa ggacgggatt ggcgatgcct gcgatgatga cgatgacaac
     2461 gacaagatcc ccgatgacag ggacaactgt ccattccatt acaacccagc ccagtatgac
     2521 tatgacagag atgatgtggg agaccgctgt gacaactgcc cctacaacca caaccctgac
     2581 caagcagaca cagacaaaaa cggggagggc gatgcctgtg ctgtggacat cgatggagat
     2641 ggaatcctca atgaacgaga caactgccag tacgtttaca acgtggacca gagggacacg
     2701 gacatggatg gggttggaga tcagtgtgac aactgccccc tggaacacaa tccagaccag
     2761 ctggactctg actcagacct cataggggac acttgtgaca acaatcagga catcgatgag
     2821 gatggccatc agaacaatct ggacaactgt ccctatgtgc ctaatgccaa ccaggccgac
     2881 catgataaag atggcaaagg agatgcctgt gaccatgacg atgacaatga cggcatccct
     2941 gatgacagag acaactgcag gctggtgccc aatcctgacc agaaggactc tgatggtgat
     3001 ggccgaggtg acgcctgcaa agacgacttt gaccatgaca atgtgccaga tattgatgac
     3061 atctgtcctg agaattttga catcagtgaa accgatttcc gacaattcca gatgattcct
     3121 ctagatccca aaggaacctc ccaaaatgac cctaactggg ttgtccgcca tcagggcaaa
     3181 gaacttgtcc agactgtaaa ctgtgaccct ggacttgctg taggttatga tgagtttaat
     3241 gctgtggact tcagcggtac cttcttcatc aacaccgaga gagatgatga ctacgctggc
     3301 tttgttttcg gctaccagtc cagcagccgc ttctacgttg tgatgtggaa acaagtcacc
     3361 cagtcctact gggacaccaa ccccacaagg gctcagggat actcaggcct gtctgtaaag
     3421 gttgtgaact ccaccaccgg ccctggcgag cacctgcgga atgcactgtg gcacacagga
     3481 aacacccctg gccaggtgcg caccctgtgg catgaccctc gccacatcgg ctggaaagat
     3541 ttcactgcat acagatggcg tctcagccac aggccaaaga ccggttatat cagagtggtg
     3601 atgtatgaag gaaagaaaat catggctgac tcgggaccca tctatgacaa aacctacgcc
     3661 ggcggtagac taggcctgtt cgtcttctct caggaaatgg tgttcttctc agacatgaaa
     3721 tacgagtgtc gagattccta atcatcagct gccaatcata accagcgctg gcaatgcacc
     3781 ttctaaaaac aagggctaga gaaacccccc acccctgccg ggatcgcctt tcctcgcctt
     3841 ccttgcctct cttcttgcat agtgtggact tgtaaagcct gagacctgcc tcaagaaaat
     3901 gcagttttca aacccagagt cagcactcgg cctttaacga atgagaatgc atcttccaag
     3961 accatgaaga gttccttggg tttgcttttg ggaaagccaa agcgcctatt tacttcccac
     4021 taggaaggtg cccgctccac tctgccttac tcacagagcc agaacttctt cgaggccacc
     4081 tctgagcagc acacacagaa gcattttcag gcatgtcaaa gaaaggaaaa aatgactcac
     4141 tagaactcac cgccaaacaa cctctgacat aggtcctgag atgtggggag gcaggagcca
     4201 aagctctagg gagggcatgt acccaagaga tgactgtatg aaaatgtgga ggagctgttc
     4261 ggtactaaat cattttcagg ggacagacag acttgctgca tttctgcatg ctgctggtga
     4321 gagctgattg acccaatctt ccacacaggc acttgagcaa gcagggaagg gagggagatc
     4381 atagcttctg gactttctcc ctttgggcac ttctcacctg cagtggccag ggtaggggtc
     4441 agaagtgtgg gccatgctgg ctgcccttga ctggtcacgc tgaaactgtt agcttattct
     4501 gcatgtacaa catgcatatg tataaggaca gctagagaac cttaccgtct cagtgagctc
     4561 tagctgcctc cgcaggaggg gcagtgcgcc tttactttat ggttagaaag gcacaaaatt
     4621 tctatcaacc taactaaaac attccttttc tttctttctt tctttctttc tttcttcctt
     4681 tttctctcag ttaccatgga tttttccaaa tctcttttgg tttttgttta acaaatgctt
     4741 taacaatgta atatatttat tttgttcccc tattctggag ggttcgtcgg aaggattgtt
     4801 actgaaacag gaagcgtggg actatctgaa tcatcttcgt agtgagtctt tatgactgta
     4861 agattgtaaa tacagattat ttattaaccc tgttctacct ggaattagac tgcatatagc
     4921 aaatgtttgc aagcaggtag ctgaggcgga tcagcaaatc tctccaaaac cagtgtgtgg
     4981 aaaggcagca gaggcagctg cttttcatct ggggctcaca gtgaatgatt tggactcctt
     5041 tggcttcccc tttgttttct ctgtagtttc aagtggaatt aggtggcggc cgcttgcatt
     5101 cactcctcct tgtaatggaa gccaggagta ctctaaggcc cacaccaccc cttgtgccta
     5161 tttccaggga gaagaaagaa aacacaatca ccttttcttt atttcatttt ttccccaaaa
     5221 gagacaaagg tggaacttct atacaaatat tacctcattt gttgtgtgac tgagtaaaga
     5281 attttggatt aagaagaaag agtttaagtg tcaataaact taaaactact gttgtgtcta
     5341 aaaagtcggt gttgtacata gcataaaaat cctttgccga ggatgatccc aagaaagaaa
     5401 cagctttgta atgttaagta caacttctca aagttctggt tttgttctat catcttttac
     5461 accgttgctt ttatttttat aaattatttt ctcgttgcca ttggaataga gatctcagac
     5521 tatgtagata tgctatttaa ataatttatc aggaaatact gcctgtagag ttagtatttc
     5581 tggtttttat atgttgcaca ctgaattgaa gaaatgttgg tttttcttgt ttcgttttag
     5641 tttgtttctt tggttttgtt tttgtttttg ctttttactt cccagttttg actctttgcc
     5701 aatacccttt ttcctaggaa cgtgcttttt tttttatttt gtacacattt ttatccattt
     5761 tacattctaa agcagtgtaa cttgtatatt actgtttctt atgtacaagg aacaacaata
     5821 aatcatatgg aagtttatat ttataaaaaa aaaaaaaaaa aa
//
