LOCUS       NM_054089               4389 bp    mRNA    linear   ROD 15-DEC-2024
DEFINITION  Mus musculus trimethylguanosine synthase 1 (Tgs1), mRNA.
ACCESSION   NM_054089
VERSION     NM_054089.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4389)
  AUTHORS   Edwin,R.K., Acharya,L.P., Maity,S.K., Chakrabarti,P., Tantia,O.,
            Joshi,M.B., Satyamoorthy,K., Parsa,K.V.L. and Misra,P.
  TITLE     TGS1/PIMT knockdown reduces lipid accumulation in adipocytes,
            limits body weight gain and promotes insulin sensitivity in mice
  JOURNAL   Biochim Biophys Acta Mol Basis Dis 1870 (1), 166896 (2024)
   PUBMED   37751782
  REMARK    GeneRIF: TGS1/PIMT knockdown reduces lipid accumulation in
            adipocytes, limits body weight gain and promotes insulin
            sensitivity in mice.
REFERENCE   2  (bases 1 to 4389)
  AUTHORS   Challa,N.L., Sarkar,A., Kapettu,S., Phanithi,P.B., Chakrabarti,P.,
            Parsa,K.V.L. and Misra,P.
  TITLE     TGS1/PIMT regulates pro-inflammatory macrophage mediated paracrine
            insulin resistance: Crosstalk between macrophages and skeletal
            muscle cells
  JOURNAL   Biochim Biophys Acta Mol Basis Dis 1870 (1), 166878 (2024)
   PUBMED   37673359
  REMARK    GeneRIF: TGS1/PIMT regulates pro-inflammatory macrophage mediated
            paracrine insulin resistance: Crosstalk between macrophages and
            skeletal muscle cells.
REFERENCE   3  (bases 1 to 4389)
  AUTHORS   Sharma,R., Maity,S.K., Chakrabarti,P., Katika,M.R., Kapettu,S.,
            Parsa,K.V.L. and Misra,P.
  TITLE     PIMT Controls Insulin Synthesis and Secretion through PDX1
  JOURNAL   Int J Mol Sci 24 (9), 8084 (2023)
   PUBMED   37175791
  REMARK    GeneRIF: PIMT Controls Insulin Synthesis and Secretion through
            PDX1.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 4389)
  AUTHORS   Blandino-Rosano,M., Romaguera Llacer,P., Lin,A., Reddy,J.K. and
            Bernal-Mizrachi,E.
  TITLE     Trimethylguanosine synthase 1 is a novel regulator of pancreatic
            beta-cell mass and function
  JOURNAL   J Biol Chem 298 (3), 101592 (2022)
   PUBMED   35041827
  REMARK    GeneRIF: Trimethylguanosine synthase 1 is a novel regulator of
            pancreatic beta-cell mass and function.
REFERENCE   5  (bases 1 to 4389)
  AUTHORS   Bedogni,F. and Hevner,R.F.
  TITLE     Cell-Type-Specific Gene Expression in Developing Mouse Neocortex:
            Intermediate Progenitors Implicated in Axon Development
  JOURNAL   Front Mol Neurosci 14, 686034 (2021)
   PUBMED   34321999
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 4389)
  AUTHORS   Sansom,S.N., Griffiths,D.S., Faedo,A., Kleinjan,D.J., Ruan,Y.,
            Smith,J., van Heyningen,V., Rubenstein,J.L. and Livesey,F.J.
  TITLE     The level of the transcription factor Pax6 is essential for
            controlling the balance between neural stem cell self-renewal and
            neurogenesis
  JOURNAL   PLoS Genet 5 (6), e1000511 (2009)
   PUBMED   19521500
REFERENCE   7  (bases 1 to 4389)
  AUTHORS   McKee,A.E., Minet,E., Stern,C., Riahi,S., Stiles,C.D. and
            Silver,P.A.
  TITLE     A genome-wide in situ hybridization map of RNA-binding proteins
            reveals anatomically restricted expression in the developing mouse
            brain
  JOURNAL   BMC Dev Biol 5, 14 (2005)
   PUBMED   16033648
  REMARK    Publication Status: Online-Only
REFERENCE   8  (bases 1 to 4389)
  AUTHORS   Enunlu,I., Papai,G., Cserpan,I., Udvardy,A., Jeang,K.T. and
            Boros,I.
  TITLE     Different isoforms of PRIP-interacting protein with
            methyltransferase domain/trimethylguanosine synthase localizes to
            the cytoplasm and nucleus
  JOURNAL   Biochem Biophys Res Commun 309 (1), 44-51 (2003)
   PUBMED   12943661
REFERENCE   9  (bases 1 to 4389)
  AUTHORS   Piao,Y., Ko,N.T., Lim,M.K. and Ko,M.S.
  TITLE     Construction of long-transcript enriched cDNA libraries from
            submicrogram amounts of total RNAs by a universal PCR amplification
            method
  JOURNAL   Genome Res 11 (9), 1553-1558 (2001)
   PUBMED   11544199
REFERENCE   10 (bases 1 to 4389)
  AUTHORS   Zhu,Y., Qi,C., Cao,W.Q., Yeldandi,A.V., Rao,M.S. and Reddy,J.K.
  TITLE     Cloning and characterization of PIMT, a protein with a
            methyltransferase domain, which interacts with and enhances nuclear
            receptor coactivator PRIP function
  JOURNAL   Proc Natl Acad Sci U S A 98 (18), 10380-10385 (2001)
   PUBMED   11517327
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK044895.1 and BC075728.1.
            
            On Apr 25, 2009 this sequence version replaced NM_054089.3.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC075728.1, AK030953.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849380, SAMN00849381
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-2392              AK044895.1         2-2393
            2393-4389           BC075728.1         2374-4370
FEATURES             Location/Qualifiers
     source          1..4389
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="4"
                     /map="4 1.99 cM"
     gene            1..4389
                     /gene="Tgs1"
                     /gene_synonym="D4Ertd800e; Ncoa6ip; Pimt"
                     /note="trimethylguanosine synthase 1"
                     /db_xref="GeneID:116940"
                     /db_xref="MGI:MGI:2151797"
     exon            1..251
                     /gene="Tgs1"
                     /gene_synonym="D4Ertd800e; Ncoa6ip; Pimt"
                     /inference="alignment:Splign:2.1.0"
     CDS             148..2709
                     /gene="Tgs1"
                     /gene_synonym="D4Ertd800e; Ncoa6ip; Pimt"
                     /note="PIPMT; nuclear receptor coactivator 6-interacting
                     protein; PRIP-interacting protein with methyltransferase
                     motif; trimethylguanosine synthase homolog; cap-specific
                     guanine-N(2) methyltransferase"
                     /codon_start=1
                     /product="trimethylguanosine synthase"
                     /protein_id="NP_473430.3"
                     /db_xref="CCDS:CCDS17938.1"
                     /db_xref="GeneID:116940"
                     /db_xref="MGI:MGI:2151797"
                     /translation="MCCEKWNHVAEMLLFIEDREEEYKILCLCSRAFVEDRKLYNLGL
                     KGYYVKSSGNNAGDQGTEEEEDGHSNGTAESHSPNESDLDSEAKLMRSMGLPIQFGRM
                     SSHENFEMSMNARNKAKVKQKRRKHQKRYLDEMVRESWRNDYEEDDLVVSDDPSSVEH
                     CENNRTCEIQSKAGSEVENLPVENTLAPKLEVPENWEKYWNEYGEGLLWQSWQEKYPD
                     QTLSSEPWNLPDTKEEWEQHYSQLYWYYLEQFQYWEAQGWTFTASQNCDKDVYTSHTE
                     VDQNAESSLKADVMTFSSSPNIVEDEIPGSNDNDHNEIITAINNITVSAEKVEQSQLD
                     SSQHCDEPLSEITGKECPASGGSDSCNGTPKENDISENRSSDQPAKELQESSGTNKGK
                     HRPHHNGADGHESDDDPPEHKPSKVKRSHELDVDENPDSEVDDNGFLLGFKHGSGQKY
                     GGIPNFSHRQVRYLEKNVKYKSKYLDLRKQMPVKSKHILFTEDSGKPFVVCKSKVRSK
                     VEKFLKWVNERVDEETSQDSLSQNKMQDTCTSSDSEEQDMSLEKADNLMETRDPEPEK
                     CQIISSATELEAEKSEVGSLVATVPENCSTEEIPNSPHAETEVEIKKKKKKNKNKKIN
                     DLPPEIASVPELAKYWAQRYRLFSRFDDGIKLDKEGWFSVTPEKIAEHIAGRVSQAFR
                     CDVVVDAFCGVGGNTIQFALTGKRVIAIDIDPVKIDLARNNAEVYGIADKIEFICGDF
                     LLLAPCLKADVVFLSPPWGGPDYATAETFDIRTMMSPDGFEIFRLSQKITNNIVYFLP
                     RNADIDQVASLAGLGGQVEIEQNFLNNKLKTITAYFGDLIRRPALLKTSTSEAEV"
     exon            252..316
                     /gene="Tgs1"
                     /gene_synonym="D4Ertd800e; Ncoa6ip; Pimt"
                     /inference="alignment:Splign:2.1.0"
     exon            317..474
                     /gene="Tgs1"
                     /gene_synonym="D4Ertd800e; Ncoa6ip; Pimt"
                     /inference="alignment:Splign:2.1.0"
     exon            475..1288
                     /gene="Tgs1"
                     /gene_synonym="D4Ertd800e; Ncoa6ip; Pimt"
                     /inference="alignment:Splign:2.1.0"
     exon            1289..1406
                     /gene="Tgs1"
                     /gene_synonym="D4Ertd800e; Ncoa6ip; Pimt"
                     /inference="alignment:Splign:2.1.0"
     exon            1407..1493
                     /gene="Tgs1"
                     /gene_synonym="D4Ertd800e; Ncoa6ip; Pimt"
                     /inference="alignment:Splign:2.1.0"
     exon            1494..1671
                     /gene="Tgs1"
                     /gene_synonym="D4Ertd800e; Ncoa6ip; Pimt"
                     /inference="alignment:Splign:2.1.0"
     exon            1672..1972
                     /gene="Tgs1"
                     /gene_synonym="D4Ertd800e; Ncoa6ip; Pimt"
                     /inference="alignment:Splign:2.1.0"
     exon            1973..2122
                     /gene="Tgs1"
                     /gene_synonym="D4Ertd800e; Ncoa6ip; Pimt"
                     /inference="alignment:Splign:2.1.0"
     exon            2123..2266
                     /gene="Tgs1"
                     /gene_synonym="D4Ertd800e; Ncoa6ip; Pimt"
                     /inference="alignment:Splign:2.1.0"
     exon            2267..2483
                     /gene="Tgs1"
                     /gene_synonym="D4Ertd800e; Ncoa6ip; Pimt"
                     /inference="alignment:Splign:2.1.0"
     exon            2484..2562
                     /gene="Tgs1"
                     /gene_synonym="D4Ertd800e; Ncoa6ip; Pimt"
                     /inference="alignment:Splign:2.1.0"
     exon            2563..4328
                     /gene="Tgs1"
                     /gene_synonym="D4Ertd800e; Ncoa6ip; Pimt"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3173..3178
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Tgs1"
                     /gene_synonym="D4Ertd800e; Ncoa6ip; Pimt"
                     /note="hexamer: AAGAAA"
     polyA_site      3197
                     /gene="Tgs1"
                     /gene_synonym="D4Ertd800e; Ncoa6ip; Pimt"
     regulatory      4294..4299
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Tgs1"
                     /gene_synonym="D4Ertd800e; Ncoa6ip; Pimt"
                     /note="hexamer: AATAAA"
     polyA_site      4328
                     /gene="Tgs1"
                     /gene_synonym="D4Ertd800e; Ncoa6ip; Pimt"
                     /note="major polyA site"
ORIGIN      
        1 gcttccggta gcgcggctgg ctggctcccg gctcgagcgt ccccaggcgc gttccgctcc
       61 tctgcccgcg cgagttacgc ctttctgtct tcagcaacag cgcccggccc agtcgcggac
      121 aacaccagag cgagcccgga taacgaaatg tgttgcgaga agtggaacca tgtggccgaa
      181 atgctgctct tcattgaaga ccgcgaggag gagtacaaga tcctttgcct ctgctcccgg
      241 gcatttgtgg aagatcgaaa attgtacaat ttgggattaa aaggctacta tgtcaaaagc
      301 agtggcaata atgcaggaga ccaaggtaca gaagaggagg aagatggtca ttccaatggc
      361 actgcagaat cacatagccc aaatgaaagt gaccttgatt ctgaggccaa actcatgaga
      421 agtatgggac taccaattca gtttggtaga atgtcttcac atgagaattt tgagatgtct
      481 atgaatgcaa gaaataaagc taaagtaaag caaaaaagaa gaaaacacca gaagcggtat
      541 ttagatgaaa tggttcggga atcctggaga aatgactatg aagaagatga ccttgtagtg
      601 tcagatgacc catcttcagt tgaacactgt gagaacaaca gaacatgtga aattcaaagc
      661 aaagcaggtt ctgaagtaga aaaccttcct gttgaaaata cactggcacc aaagctagag
      721 gtcccagaga attgggaaaa gtactggaat gaatatggag aagggctgtt gtggcaaagc
      781 tggcaggaga agtatccaga ccagacactg tcttctgagc cttggaacct ccctgataca
      841 aaggaagaat gggaacaaca ttacagtcag ctttattggt attatttgga acaatttcag
      901 tattgggaag ctcagggctg gacttttact gcctcacaaa actgtgataa agatgtttac
      961 acatctcaca cagaagtaga ccagaatgct gagagcagcc tgaaagcaga tgtaatgact
     1021 ttttcatctt cacctaatat agttgaggat gaaatccctg gctcaaatga taatgaccac
     1081 aatgaaatta ttactgcgat taacaacata actgtgagtg cagagaaagt agaacagagc
     1141 cagttagatt cctctcaaca ctgtgacgag ccgctcagtg aaatcactgg gaaagagtgc
     1201 cctgcctctg gaggaagtga ctcctgtaat ggaacaccca aagaaaatga tatatctgag
     1261 aacagaagct cagaccaacc agctaaagag ttgcaggagt cttcaggaac aaataaaggt
     1321 aaacatagac cacaccataa tggggctgat ggacatgaga gtgacgatga cccacctgag
     1381 cataagccca gcaaagtgaa gagaagccat gaattggatg ttgatgaaaa cccagattca
     1441 gaagttgatg acaatggttt ccttctagga ttcaagcatg gctcaggaca aaaatatggt
     1501 ggaattccaa atttcagcca tcgacaggtc aggtatctag agaagaatgt gaaatacaaa
     1561 tcaaagtacc tggatctgcg caagcagatg cctgtgaaga gcaagcacat cctgttcacc
     1621 gaggactcag ggaaaccttt tgtcgtgtgt aagagtaaag tacggagtaa ggtagaaaaa
     1681 ttcctaaaat gggttaatga acgagtagat gaggagacat cacaggattc tctttctcaa
     1741 aacaaaatgc aagacacttg cacaagcagt gactcagagg aacaagacat gtccttggag
     1801 aaagctgaca acctgatgga gaccagagat ccagaacctg aaaaatgtca gatcatctct
     1861 tcagccactg aacttgaagc agaaaagagt gaagtaggct ccttagtagc aactgttcca
     1921 gagaactgtt caactgaaga aataccaaac tctccccatg cagaaacaga agttgaaata
     1981 aagaagaaaa agaagaagaa caagaacaaa aagataaatg atctaccacc tgaaatagct
     2041 tctgttccag agctggctaa atactgggcc cagagataca ggctcttctc tcgctttgat
     2101 gatggaatca aattggacaa agagggctgg ttttcagtca ctcctgagaa gattgctgaa
     2161 cacattgctg gccgtgtcag tcaggccttc agatgtgacg ttgtagtaga tgctttctgt
     2221 ggagttggag gaaataccat tcagtttgcc ttaaccggga aaagagtgat tgctattgat
     2281 atcgaccctg ttaaaattga tcttgctcgg aataatgctg aagtttatgg gatagcagat
     2341 aaaatagagt ttatctgtgg agatttcctg ctcttggctc catgtttaaa agctgatgtt
     2401 gtgttcctaa gtccaccttg gggagggcca gattatgcta ctgcagagac cttcgacatt
     2461 aggacaatga tgtctcctga tggctttgaa attttcaggc tttcccagaa gatcactaat
     2521 aatattgttt attttcttcc aagaaatgct gatattgacc aggtggcatc cttggctggg
     2581 cttggaggac aagtggaaat agaacagaac tttcttaaca acaagttaaa gaccatcact
     2641 gcatactttg gtgacctgat cagaagacca gcacttctga agaccagcac ttctgaagcg
     2701 gaagtgtgat actgtaaagc acagagagat catgtggagt gaggatgtat gacattcaag
     2761 tgagagacag gaggaaggcc ttcctaggtt ctttaatact gtgtgcacat gtgatgtgga
     2821 aaactccgag ttttaaagga tgctaatgaa gtggtttgag atctttaggt aatggtaatg
     2881 gatctgtgtt gtacacttta aaacagcatc agggcaaggt gatagtggtg cctgcaatcc
     2941 cagcacttca gaaagatcat gagttcaagc tcatgctgtg cttcctaaca aatcaaggct
     3001 ggcctgagct acatgagatc ttgccacaaa aaagaaaaaa atcataaaaa gtctaggcat
     3061 atccctgtgc ttaaattaag tacatctgta tatgtatttg ttttcattct ttaactgttt
     3121 tagcttaaca tctttgtgtc tgtgtaagta attattagtt ttttaaaata taaagaaaaa
     3181 caaagcaatt ctacctacac gtgtaatccc tattgaaata atgttaccaa tttaatgcta
     3241 acacagaaaa agtaaaactc ccaccaccta acctaccttc ttttcagcta tctaaagcta
     3301 atcacagtta gcaggacttt gctttttaac ttttttatta catttttatt ttgtctatat
     3361 gtgcatgcat gcgtgtgtgt agaggtcaga ggacaacttg ctagagtcca ttctctactt
     3421 acaccatgtg ggttcctggg aatcaagtta aagtcattag actttataac cagagccttt
     3481 acctactgag ccaacccatt ggtcccctct taattcttct gaagaaaaga tcgcagtata
     3541 acagtgtacc ccggtgcact tctccattga tgcactcact gtgcgttcat tgccttccct
     3601 ttgtggatac ttaatgtata gtatatgaaa taattgcttt tgtgagagag agaatgtgtt
     3661 tgtgcgcaag agagatgtgc gtgtgtgcat gtgtgtgcgc ctactttgac ccaagtccag
     3721 ggaagagtgt tggattcctt atagctggag ttacagatgg ttatgagctg tcaaatgtgg
     3781 gtactgggaa atgcacttgg gtcctctgga agtggaagta agttctctct ccagcccaag
     3841 atgggctttt taatcagtgt ttttgctttt ctgttttggt atggttttgt tgttattgtt
     3901 ttgttttgtc ttgttttgtt tttttgtata cccaggctgg cctcatactt tgtgtgtaac
     3961 tcagagtgac tttgagctcc taacccttct ggctccacct actgcacctg gcttatcaga
     4021 gtatcttgaa agccattcta tttcagttca tacatgtcaa tctacctcgt gttttaaatg
     4081 gctattttgt attccatcat ctatatatat aaactagttc cctactgaga ggcacccaag
     4141 ctgtttttat ggttttatcg ttaccaactg tttatgcact gtctttcctt acaatatttg
     4201 ctgttgaaca agtatttcag ttggtaagtt tctagaagtt acaccactgg gcttttgtac
     4261 atttcagtct cgataaaaag actaaattgc ttcaataaaa ttgaacttgc ttttaggctt
     4321 atctcaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
     4381 aaaaaaaaa
//
