LOCUS       NM_177911               2767 bp    mRNA    linear   ROD 03-JUN-2024
DEFINITION  Mus musculus transglutaminase 4 (prostate) (Tgm4), mRNA.
ACCESSION   NM_177911 XM_914603
VERSION     NM_177911.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2767)
  AUTHORS   Lough-Stevens,M., Ghione,C.R., Urness,M., Hobbs,A., Sweeney,C.M.
            and Dean,M.D.
  TITLE     Male-derived copulatory plugs enhance implantation success in
            female Mus musculus
  JOURNAL   Biol Reprod 104 (3), 684-694 (2021)
   PUBMED   33355341
REFERENCE   2  (bases 1 to 2767)
  AUTHORS   Mangels,R., Tsung,K., Kwan,K. and Dean,M.D.
  TITLE     Copulatory plugs inhibit the reproductive success of rival males
  JOURNAL   J Evol Biol 29 (11), 2289-2296 (2016)
   PUBMED   27488082
REFERENCE   3  (bases 1 to 2767)
  AUTHORS   Landegren,N., Sharon,D., Shum,A.K., Khan,I.S., Fasano,K.J.,
            Hallgren,A., Kampf,C., Freyhult,E., Ardesjo-Lundgren,B.,
            Alimohammadi,M., Rathsman,S., Ludvigsson,J.F., Lundh,D.,
            Motrich,R., Rivero,V., Fong,L., Giwercman,A., Gustafsson,J.,
            Perheentupa,J., Husebye,E.S., Anderson,M.S., Snyder,M. and Kampe,O.
  TITLE     Transglutaminase 4 as a prostate autoantigen in male subfertility
  JOURNAL   Sci Transl Med 7 (292), 292ra101 (2015)
   PUBMED   26084804
  REMARK    GeneRIF: TGM4 is a prostatic secretory molecule with critical role
            in male reproduction.
REFERENCE   4  (bases 1 to 2767)
  AUTHORS   Dean,M.D.
  TITLE     Genetic disruption of the copulatory plug in mice leads to severely
            reduced fertility
  JOURNAL   PLoS Genet 9 (1), e1003185 (2013)
   PUBMED   23341775
  REMARK    GeneRIF: male mice knockouts for transglutaminase IV (Tgm4) fail to
            form a copulatory plug, demonstrating that this gene is necessary
            for plug formation and lending a powerful new genetic tool to begin
            characterizing plug function
REFERENCE   5  (bases 1 to 2767)
  AUTHORS   Johnson,K.B., Petersen-Jones,H., Thompson,J.M., Hitomi,K., Itoh,M.,
            Bakker,E.N., Johnson,G.V., Colak,G. and Watts,S.W.
  TITLE     Vena cava and aortic smooth muscle cells express transglutaminases
            1 and 4 in addition to transglutaminase 2
  JOURNAL   Am J Physiol Heart Circ Physiol 302 (7), H1355-H1366 (2012)
   PUBMED   22307675
  REMARK    GeneRIF: These studies demonstrate that tranglutaminase 2
            independent activity exists in the vasculature and that Tgm1 and
            tgm4 are expressed in vascular tissues
REFERENCE   6  (bases 1 to 2767)
  AUTHORS   Tseng,H.C., Lin,H.J., Tang,J.B., Gandhi,P.S., Chang,W.C. and
            Chen,Y.H.
  TITLE     Identification of the major TG4 cross-linking sites in the
            androgen-dependent SVS I exclusively expressed in mouse seminal
            vesicle
  JOURNAL   J Cell Biochem 107 (5), 899-907 (2009)
   PUBMED   19452452
  REMARK    GeneRIF: The androgen-dependent SVS I is exclusively expressed in
            the luminal epithelium of seminal vesicle; Gln232 and Gln254 are
            the major TG4-reactive residues on SVS I and and TG4 is much
            stronger than TG2 to cross-link the glutamine residues in SVS I.
REFERENCE   7  (bases 1 to 2767)
  AUTHORS   Joesting,M.S., Cheever,T.R., Volzing,K.G., Yamaguchi,T.P., Wolf,V.,
            Naf,D., Rubin,J.S. and Marker,P.C.
  TITLE     Secreted frizzled related protein 1 is a paracrine modulator of
            epithelial branching morphogenesis, proliferation, and secretory
            gene expression in the prostate
  JOURNAL   Dev Biol 317 (1), 161-173 (2008)
   PUBMED   18371946
REFERENCE   8  (bases 1 to 2767)
  AUTHORS   Setiady,Y.Y., Ohno,K., Samy,E.T., Bagavant,H., Qiao,H., Sharp,C.,
            She,J.X. and Tung,K.S.
  TITLE     Physiologic self antigens rapidly capacitate autoimmune
            disease-specific polyclonal CD4+ CD25+ regulatory T cells
  JOURNAL   Blood 107 (3), 1056-1062 (2006)
   PUBMED   16223778
REFERENCE   9  (bases 1 to 2767)
  AUTHORS   Piao,Y., Ko,N.T., Lim,M.K. and Ko,M.S.
  TITLE     Construction of long-transcript enriched cDNA libraries from
            submicrogram amounts of total RNAs by a universal PCR amplification
            method
  JOURNAL   Genome Res 11 (9), 1553-1558 (2001)
   PUBMED   11544199
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK035279.1 and AF486627.1.
            
            On Apr 25, 2009 this sequence version replaced NM_177911.3.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK035279.1, AF486627.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-469               AK035279.1         2-470
            470-1278            AF486627.1         469-1277
            1279-1650           AK035279.1         1280-1651
            1651-1736           AF486627.1         1650-1735
            1737-2767           AK035279.1         1738-2768
FEATURES             Location/Qualifiers
     source          1..2767
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="9"
                     /map="9 73.56 cM"
     gene            1..2767
                     /gene="Tgm4"
                     /gene_synonym="9530008N10Rik; Eapa1; TGP"
                     /note="transglutaminase 4 (prostate)"
                     /db_xref="GeneID:331046"
                     /db_xref="MGI:MGI:3027002"
     exon            1..85
                     /gene="Tgm4"
                     /gene_synonym="9530008N10Rik; Eapa1; TGP"
                     /inference="alignment:Splign:2.1.0"
     CDS             70..2082
                     /gene="Tgm4"
                     /gene_synonym="9530008N10Rik; Eapa1; TGP"
                     /EC_number="2.3.2.13"
                     /note="TGase-4; transglutaminase-4; experimental
                     autoimmune prostatitis antigen 1"
                     /codon_start=1
                     /product="protein-glutamine gamma-glutamyltransferase 4"
                     /protein_id="NP_808579.2"
                     /db_xref="CCDS:CCDS23653.1"
                     /db_xref="GeneID:331046"
                     /db_xref="MGI:MGI:3027002"
                     /translation="MDSRNVLIIYAVNVERKLNAAAHHTSEYQTKKLVLRRGQIFTLK
                     VILNRPLQPQDELKVTFTSGQRDPPYMVELDPVTSYRSKGWQVKIAKQSGVEVILNVI
                     SAADAVVGRYKMRVNEYKAGVFYLLFNPWCSDDSVFMASEEERAEYILNDTGYMYMGF
                     AKQIKEKPWTFGQFEKHILSCCFNLLFQLENNEMQNPVLVSRAICTMMCAANGGVLMG
                     NWTGDYADGTAPYVWTSSVPILQQHYVTRMPVRYGQCWVFSGILTTALRAVGIPARSV
                     TNFESAHDTEKNLTVDIYLDESGKTIPHLTKDSVWNFHVWTDAWMKRQDLPHGYDGWQ
                     VLDSTPQEISDGGFRTGPSPLTAIRQGLIQMKYDTTFVFTEVNGDKFIWLVKQNQERE
                     KNILIAVETASIGKKISTKMVGENRREDITLQYKFPEGSPEERKVMAKASGKPSDDKL
                     NSRTLNNSLQISVLQNSLELGAPIYLTITLKRKTATPQNVNISCSLNLQTYTGNKKTN
                     LGVIQKTVQIHGQESRVFLTMDASYYIYKLGMVDDEMVIGGFIIAEIVDSGERVATDT
                     TLCFLYSAFSVEMPSTGKVKQPLVITSKFTNTLPIPLTNIKFSVESLGLANMKSWEQE
                     TVPPGKTITFQMECTPVKAGPQKFIVKFISRQVKEVHAEKVVLISK"
     exon            86..259
                     /gene="Tgm4"
                     /gene_synonym="9530008N10Rik; Eapa1; TGP"
                     /inference="alignment:Splign:2.1.0"
     exon            260..357
                     /gene="Tgm4"
                     /gene_synonym="9530008N10Rik; Eapa1; TGP"
                     /inference="alignment:Splign:2.1.0"
     exon            358..469
                     /gene="Tgm4"
                     /gene_synonym="9530008N10Rik; Eapa1; TGP"
                     /inference="alignment:Splign:2.1.0"
     exon            470..588
                     /gene="Tgm4"
                     /gene_synonym="9530008N10Rik; Eapa1; TGP"
                     /inference="alignment:Splign:2.1.0"
     exon            589..690
                     /gene="Tgm4"
                     /gene_synonym="9530008N10Rik; Eapa1; TGP"
                     /inference="alignment:Splign:2.1.0"
     exon            691..862
                     /gene="Tgm4"
                     /gene_synonym="9530008N10Rik; Eapa1; TGP"
                     /inference="alignment:Splign:2.1.0"
     exon            863..1001
                     /gene="Tgm4"
                     /gene_synonym="9530008N10Rik; Eapa1; TGP"
                     /inference="alignment:Splign:2.1.0"
     exon            1002..1105
                     /gene="Tgm4"
                     /gene_synonym="9530008N10Rik; Eapa1; TGP"
                     /inference="alignment:Splign:2.1.0"
     exon            1106..1357
                     /gene="Tgm4"
                     /gene_synonym="9530008N10Rik; Eapa1; TGP"
                     /inference="alignment:Splign:2.1.0"
     exon            1358..1633
                     /gene="Tgm4"
                     /gene_synonym="9530008N10Rik; Eapa1; TGP"
                     /inference="alignment:Splign:2.1.0"
     exon            1634..1803
                     /gene="Tgm4"
                     /gene_synonym="9530008N10Rik; Eapa1; TGP"
                     /inference="alignment:Splign:2.1.0"
     exon            1804..1940
                     /gene="Tgm4"
                     /gene_synonym="9530008N10Rik; Eapa1; TGP"
                     /inference="alignment:Splign:2.1.0"
     exon            1941..2767
                     /gene="Tgm4"
                     /gene_synonym="9530008N10Rik; Eapa1; TGP"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2741..2746
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Tgm4"
                     /gene_synonym="9530008N10Rik; Eapa1; TGP"
                     /note="hexamer: ATTAAA"
     polyA_site      2761
                     /gene="Tgm4"
                     /gene_synonym="9530008N10Rik; Eapa1; TGP"
                     /note="major polyA site"
ORIGIN      
        1 atgattctgg cagtgccttt ggcatcagac tagagtcttg acagggctgc agagagaggt
       61 agcaggacca tggactccag gaatgtgctt atcatttacg ctgtgaatgt ggagagaaag
      121 ctgaatgctg ctgcccacca cacatccgag taccagacca agaagctcgt gctgaggcgt
      181 ggacagatat tcactctgaa ggtgatactg aaccgacctc tccaacctca ggatgagctg
      241 aaggtgacat tcacttccgg acagagagac ccaccctaca tggtggagct tgatccagtg
      301 acatcctacc gttctaaggg ctggcaggtg aagattgcca aacagtctgg cgtagaggtt
      361 attctgaatg tcatcagtgc tgctgacgcc gtagtgggaa ggtacaagat gagggtgaac
      421 gagtataaag ctggagtatt ctaccttctc ttcaatccgt ggtgctcaga cgacagtgtc
      481 ttcatggcta gtgaggaaga acgagcagag tacatcctta atgacaccgg ctacatgtac
      541 atgggtttcg ccaaacaaat taaagagaag ccctggacct ttggtcagtt tgagaagcac
      601 atcctgagct gctgcttcaa cttgttgttc cagttggaga acaatgagat gcagaaccca
      661 gtgcttgtgt ccagggccat ctgtactatg atgtgtgctg cgaacggcgg agtgctgatg
      721 gggaactgga caggagacta tgccgatggc acggccccat atgtgtggac cagcagtgta
      781 cccatcctgc agcagcatta tgtcaccagg atgcctgtac gctacggcca gtgctgggtt
      841 ttctctggaa tcctgaccac agcactgaga gcagtgggta tcccagctcg aagcgtgact
      901 aactttgagt cagcccatga tacagaaaag aacctcacag tagacatcta cctggatgaa
      961 tcgggcaaga caattccaca tctgaccaaa gactctgtct ggaatttcca cgtgtggaca
     1021 gatgcctgga tgaaaaggca ggatctacct catggctatg atggctggca ggtcctggat
     1081 tctacacccc aggaaatcag cgatggaggc ttccgcaccg ggccttcacc actgactgct
     1141 attcgccagg gattgatcca gatgaagtat gacaccacgt ttgtgttcac ggaggtcaat
     1201 ggtgacaagt ttatctggtt ggtgaagcag aaccaggaaa gggagaagaa tattctcatc
     1261 gccgtggaga ctgccagcat cggcaagaaa atcagcacca agatggtggg cgagaacagg
     1321 cgtgaagata taaccttgca gtacaagttc ccagaaggca gcccagagga gaggaaggtc
     1381 atggcgaagg cttctggtaa accttctgat gacaagctca actctcggac tctgaacaac
     1441 tctcttcaga tatctgtcct ccagaactcg ttggagttag gagctcccat ctatttgacc
     1501 ataactttga aaagaaaaac cgccacccca cagaatgtca acatttcctg ctccctcaac
     1561 ctgcagacat acacaggcaa caagaagaca aacctgggag tcatccagaa gactgtgcag
     1621 atccacggcc aagaatcaag ggtgtttctc accatggatg ccagctacta catctataaa
     1681 ctgggcatgg ttgatgatga gatggtcatt ggagggttca tcattgcaga aattgtagat
     1741 tctggtgaaa gggtggccac tgatacaacc ctgtgtttcc tgtactctgc cttctctgta
     1801 gagatgccga gcacaggcaa ggtcaagcag cctctcgtca tcaccagcaa attcacgaac
     1861 accctgccca tccctttgac taacatcaag ttctcagtgg aaagcctggg cctcgctaac
     1921 atgaaatcct gggaacaaga gacagtgcca cctggaaaga ccatcacttt ccaaatggaa
     1981 tgcaccccag tgaaagctgg accccagaaa ttcattgtca agtttatttc cagacaagtg
     2041 aaagaggttc atgccgagaa ggtcgttctc atcagcaagt agcacatttg ataccatgga
     2101 gatggaggca ggagccctga ttggctctga gccttggcta agcatgttaa cattttctaa
     2161 attgcattta gctttcaggt cagggatggt taaatttgat tatgcgtgtg aaggcacatt
     2221 caagagccag cccagcaaga cagagctcaa aggccgatgt caccacaggc agtagctcca
     2281 gggagccaga gaacctgggc acatgtcaca ggcaaacatt tcaaatacat atttaggaat
     2341 tgtggcctca gaagacagtg ccaccgtcat ctggattcag aaccatgtac agagcccaga
     2401 tatagaaggg ctggaggcat cttccatcta gaccatggat tctatcaagt ccaggggtgc
     2461 ctggaggctg tgctggaagt cttatagaaa gcatcttgga gctggtcctg ggtcaggcac
     2521 tacaccctct tatcccaagg ctctgtcctc ttagaagcag gccccacgct ggcgtgaggc
     2581 cctcttgctc tggatgagcc atgggaggta gacccttttt ttaagtaaga gtctatggcc
     2641 agtgacaaca tctgaagaag caacgtatcc tgccctgttc tcgggagtct gtgctttgct
     2701 ctgctgttaa ggtatctgct cacataccac tatgctttga attaaactct ggcaacttta
     2761 agatggc
//
