LOCUS       NM_008249               2464 bp    mRNA    linear   ROD 22-DEC-2022
DEFINITION  Mus musculus transcription factor B2, mitochondrial (Tfb2m),
            transcript variant 1, mRNA; nuclear gene for mitochondrial product.
ACCESSION   NM_008249
VERSION     NM_008249.5
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2464)
  AUTHORS   Son JS, Chae SA, Zhao L, Wang H, de Avila JM, Zhu MJ, Jiang Z and
            Du M.
  TITLE     Maternal exercise intergenerationally drives muscle-based
            thermogenesis via activation of apelin-AMPK signaling
  JOURNAL   EBioMedicine 76, 103842 (2022)
   PUBMED   35081489
REFERENCE   2  (bases 1 to 2464)
  AUTHORS   Alsina D, Lytovchenko O, Schab A, Atanassov I, Schober FA, Jiang M,
            Koolmeister C, Wedell A, Taylor RW, Wredenberg A and Larsson NG.
  TITLE     FBXL4 deficiency increases mitochondrial removal by autophagy
  JOURNAL   EMBO Mol Med 12 (7), e11659 (2020)
   PUBMED   32525278
REFERENCE   3  (bases 1 to 2464)
  AUTHORS   Nicholas LM, Valtat B, Medina A, Andersson L, Abels M, Mollet IG,
            Jain D, Eliasson L, Wierup N, Fex M and Mulder H.
  TITLE     Mitochondrial transcription factor B2 is essential for
            mitochondrial and cellular function in pancreatic beta-cells
  JOURNAL   Mol Metab 6 (7), 651-663 (2017)
   PUBMED   28702322
  REMARK    GeneRIF: Loss of Tfb2m in pancreatic beta-cells results in
            progressive mitochondrial dysfunction and impaired insulin
            secretion in response to metabolic stimuli.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2464)
  AUTHORS   Gomez-Del Arco P, Perdiguero E, Yunes-Leites PS, Acin-Perez R,
            Zeini M, Garcia-Gomez A, Sreenivasan K, Jimenez-Alcazar M, Segales
            J, Lopez-Maderuelo D, Ornes B, Jimenez-Borreguero LJ, D'Amato G,
            Enshell-Seijffers D, Morgan B, Georgopoulos K, Islam AB, Braun T,
            de la Pompa JL, Kim J, Enriquez JA, Ballestar E, Munoz-Canoves P
            and Redondo JM.
  TITLE     The Chromatin Remodeling Complex Chd4/NuRD Controls Striated Muscle
            Identity and Metabolic Homeostasis
  JOURNAL   Cell Metab 23 (5), 881-892 (2016)
   PUBMED   27166947
REFERENCE   5  (bases 1 to 2464)
  AUTHORS   Lodeiro MF, Uchida A, Bestwick M, Moustafa IM, Arnold JJ, Shadel GS
            and Cameron CE.
  TITLE     Transcription from the second heavy-strand promoter of human mtDNA
            is repressed by transcription factor A in vitro
  JOURNAL   Proc Natl Acad Sci U S A 109 (17), 6513-6518 (2012)
   PUBMED   22493245
REFERENCE   6  (bases 1 to 2464)
  AUTHORS   Gaspari M, Falkenberg M, Larsson NG and Gustafsson CM.
  TITLE     The mitochondrial RNA polymerase contributes critically to promoter
            specificity in mammalian cells
  JOURNAL   EMBO J 23 (23), 4606-4614 (2004)
   PUBMED   15526033
REFERENCE   7  (bases 1 to 2464)
  AUTHORS   Marikawa Y, Fujita TC and Alarcon VB.
  TITLE     An enhancer-trap LacZ transgene reveals a distinct expression
            pattern of Kinesin family 26B in mouse embryos
  JOURNAL   Dev Genes Evol 214 (2), 64-71 (2004)
   PUBMED   14727108
REFERENCE   8  (bases 1 to 2464)
  AUTHORS   Rantanen A, Gaspari M, Falkenberg M, Gustafsson CM and Larsson NG.
  TITLE     Characterization of the mouse genes for mitochondrial transcription
            factors B1 and B2
  JOURNAL   Mamm Genome 14 (1), 1-6 (2003)
   PUBMED   12532263
  REMARK    GeneRIF: Metazoans have two TFBM genes, suggesting that a
            duplication of the gene has permitted a more flexible regulation of
            mtDNA.
REFERENCE   9  (bases 1 to 2464)
  AUTHORS   Piao Y, Ko NT, Lim MK and Ko MS.
  TITLE     Construction of long-transcript enriched cDNA libraries from
            submicrogram amounts of total RNAs by a universal PCR amplification
            method
  JOURNAL   Genome Res 11 (9), 1553-1558 (2001)
   PUBMED   11544199
REFERENCE   10 (bases 1 to 2464)
  AUTHORS   Brady KP, Rowe LB, Her H, Stevens TJ, Eppig J, Sussman DJ, Sikela J
            and Beier DR.
  TITLE     Genetic mapping of 262 loci derived from expressed sequences in a
            murine interspecific cross using single-strand conformational
            polymorphism analysis
  JOURNAL   Genome Res 7 (11), 1085-1093 (1997)
   PUBMED   9371744
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC130279.5, AK166717.1 and AA434719.1.
            
            [WARNING] On Apr 27, 2023 this sequence was replaced by
            NM_008249.6.
            
            On Sep 5, 2016 this sequence version replaced NM_008249.4.
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (1).
            
            Sequence Note:.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK166717.1, AK168970.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164139 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: reported by MitoCarta
            RefSeq Select criteria             :: based on conservation,
                                                  expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-22                AC130279.5         158731-158752       c
            23-2455             AK166717.1         1-2433
            2456-2464           AA434719.1         552-560
FEATURES             Location/Qualifiers
     source          1..2464
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="1"
                     /map="1 83.56 cM"
     gene            1..2464
                     /gene="Tfb2m"
                     /gene_synonym="Hkp1"
                     /note="transcription factor B2, mitochondrial"
                     /db_xref="GeneID:15278"
                     /db_xref="MGI:MGI:107937"
     exon            1..455
                     /gene="Tfb2m"
                     /gene_synonym="Hkp1"
                     /inference="alignment:Splign:2.1.0"
     CDS             146..1336
                     /gene="Tfb2m"
                     /gene_synonym="Hkp1"
                     /note="isoform 1 is encoded by transcript variant 1;
                     dimethyladenosine transferase 2, mitochondrial; mTFB2M;
                     mtTFB2; mitochondrial 12S rRNA dimethylase 2;
                     mitochondrial transcription factor B2;
                     S-adenosylmethionine-6-N', N'-adenosyl(rRNA)
                     dimethyltransferase 2; house-keeping protein 1"
                     /codon_start=1
                     /product="dimethyladenosine transferase 2, mitochondrial
                     isoform 1"
                     /protein_id="NP_032275.2"
                     /db_xref="CCDS:CCDS15561.1"
                     /db_xref="GeneID:15278"
                     /db_xref="MGI:MGI:107937"
                     /translation="MRGPAMRLPPRIALSALARGPSCILGSGAATRKDWQTRNRRGFS
                     DFNIEPLPDSDLEESSPWTSRNRSEPTRHIACKKAARNLVRDLLEHQNPSRQIILECN
                     PGPGILTGALLKAGARVVAFESEKTFIPHLEPLQRNMDGELQVVHCDFFKMDPRYQEV
                     VRPDVSSQAIFQNLGIKAVPWSAGVPIKVFGILPYKHERRILWKILFDLYSCESIYRY
                     GRVELNMFVSEKEFRKLIATPKRPDLYQVMAVLWQVACDVKFLHMEPWSSFSVHTENG
                     HLEKSKHGESVNLLKQNLYLVRMTPRRTLFTENLSPLNYDIFFHLVKHCFGKRNAPII
                     RHLRSLSTVDPINILRQIRKNPGDTAARMYPHDFKKLFETIEQSEDSVFKWIYDYCPE
                     DMEF"
     exon            456..544
                     /gene="Tfb2m"
                     /gene_synonym="Hkp1"
                     /inference="alignment:Splign:2.1.0"
     exon            545..695
                     /gene="Tfb2m"
                     /gene_synonym="Hkp1"
                     /inference="alignment:Splign:2.1.0"
     exon            696..844
                     /gene="Tfb2m"
                     /gene_synonym="Hkp1"
                     /inference="alignment:Splign:2.1.0"
     exon            845..934
                     /gene="Tfb2m"
                     /gene_synonym="Hkp1"
                     /inference="alignment:Splign:2.1.0"
     exon            935..997
                     /gene="Tfb2m"
                     /gene_synonym="Hkp1"
                     /inference="alignment:Splign:2.1.0"
     exon            998..1158
                     /gene="Tfb2m"
                     /gene_synonym="Hkp1"
                     /inference="alignment:Splign:2.1.0"
     exon            1159..2459
                     /gene="Tfb2m"
                     /gene_synonym="Hkp1"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 ggctaggtcg ttcgcatgcg ccattcgtaa tgtttacttc cgcccggcct agtgtggtgg
       61 tgaaatgtgt gcacgtgtga tggggtgctc tgcgttcttc gcagaggcta ccttaactgg
      121 tgtttgaggt ggactttaga ggaggatgag gggaccggcg atgcggcttc ccccgcggat
      181 cgcgctgtca gcgttggccc gcggcccatc ttgcattcta gggtccggag cggcgacgcg
      241 gaaggactgg caaacgagga accgccgcgg cttctctgac ttcaatatag agccgttgcc
      301 tgattctgat ttggaggagt cgtccccgtg gacatccagg aatcgatcgg agcctacgcg
      361 tcacatagca tgtaagaaag cggccaggaa cctggtacgg gacctgctgg agcaccaaaa
      421 cccatcccgt caaattatac tagagtgcaa tccaggtcct ggaatcctga ctggggcatt
      481 acttaaagct ggtgccagag tggttgcctt tgaaagtgaa aagactttta ttccacattt
      541 ggagccctta cagaggaaca tggatggaga gctacaggtg gttcactgtg acttctttaa
      601 aatggatcct agatatcagg aagtagtaag accagatgtg agttcacagg caatatttca
      661 gaacctggga ataaaagcag ttccttggtc agcaggtgtt cctataaaag tatttggaat
      721 cctaccatat aaacatgaaa gacgaatact ttggaaaatt ctgttcgatc tgtactcctg
      781 cgaatctata tacagatatg gacgagtaga actgaatatg tttgttagtg aaaaagaatt
      841 ccggaaactg atagcgactc ctaaaagacc ggatttatat caagtaatgg ctgttctttg
      901 gcaagtggcc tgtgacgtta agttcctgca catggaacct tggtcgtcat ttagtgtgca
      961 tacggaaaat gggcacttag aaaagtcaaa gcacggggaa tcagtaaatt tactgaaaca
     1021 aaacctatat cttgttcgaa tgactcctcg taggacttta tttacagaaa acctaagtcc
     1081 tttgaactat gatatatttt tccacttggt aaagcattgc tttgggaaac gcaatgcccc
     1141 aataatacgc catttacgtt cactgagtac ggttgatcct attaatatcc tgaggcagat
     1201 aagaaaaaac cctggagaca cagctgctag aatgtatcct cacgacttca aaaaactttt
     1261 tgaaaccata gagcaatcgg aagactccgt ctttaagtgg atctatgact actgcccgga
     1321 ggacatggag ttctaggaag agtctgcaag ccgagagtga gctggagctg tttgtccatt
     1381 attttgaaat catgtgacaa atgaacactg aatttcaaga agttcatatc cttttaacag
     1441 aagagaaact acccttgttt ggcacaaatg aagcaaataa tttcttctca agttgatacc
     1501 tttgatacac agaaaacaca gtggctgcag tattattcaa agttaaataa aacgaaaacc
     1561 tgtcagtttg atcagatcaa atctactttg ttttaaagta ctctctgccg tagatcaatg
     1621 gtagaacaca tacctagtat gtccaaggtt ttggttttgc tcccttgtag acgcccgact
     1681 cgccagcaag aatgacgcca caacaggatt cttttgcaca cgtttattgg gagagcttga
     1741 tagcgaaagc gaaagacccc gagcccaaaa aaccagtgct gcttatatag gcctaggata
     1801 ggtgtgtcta cacctgattg gttatgcttt cagtacctca tttacatgcc ttgggccagg
     1861 cagtgactct gcaaaaaaaa aaaaaactct actgcacatg cgcacattgg ttgtttaccc
     1921 atattcatgg gcgtcagctt attgaaacca gtaccatctt gtaatggcgt ttattggctt
     1981 cccatactcc ctagcacagg aaatttttaa aaaatttcta tttactgtaa aggcccatag
     2041 gtatggagta ttttcaattg taatttaatt tactttaaaa aaacaaaaaa cctttgggcc
     2101 aaacctcttt aaatatgcac ttttcaccat tgccaattgc ttgcatctcc ctcagtttct
     2161 atactgttac gcttagaaaa taaattgatt tgggcagaca tggtggcata agtcccaggc
     2221 cttgtgaggt ggagggccca tcagtttaat gccactttca actgtagaat tgagtttgag
     2281 accagtttag gctaagaaaa gaccctgtca agcaaaacaa cggtataaac ttagaaagtg
     2341 atcagtttat cttttattat gttgaagtgg attatcaggc actatgcaat ccagttgttt
     2401 aaattttaaa ttgtagtatg ttttaaaact ttttgagaaa taaaagaatt tgtatataga
     2461 aaaa
//
