LOCUS       NM_031386               4776 bp    mRNA    linear   ROD 11-MAR-2025
DEFINITION  Mus musculus testis expressed gene 14 intercellular bridge forming
            factor (Tex14), transcript variant 2, mRNA.
ACCESSION   NM_031386
VERSION     NM_031386.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4776)
  AUTHORS   He,Y., Wang,H., Hong,T., Hu,L., Gao,C., Gao,L., Cui,Y., Tan,R.,
            Pu,D. and Wu,J.
  TITLE     HFM1 is essential for the germ cell intercellular bridge transport
            in primordial follicle formation in mice
  JOURNAL   Cell Mol Life Sci 82 (1), 28 (2024)
   PUBMED   39725823
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4776)
  AUTHORS   Zhang,J., Ruiz,M., Bergh,P.O., Henricsson,M., Stojanovic,N.,
            Devkota,R., Henn,M., Bohlooly-Y,M., Hernandez-Hernandez,A.,
            Alsheimer,M., Boren,J., Pilon,M. and Shibuya,H.
  TITLE     Regulation of meiotic telomere dynamics through membrane fluidity
            promoted by AdipoR2-ELOVL2
  JOURNAL   Nat Commun 15 (1), 2315 (2024)
   PUBMED   38485951
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 4776)
  AUTHORS   Ikami,K., Nuzhat,N., Abbott,H., Pandoy,R., Haky,L., Spradling,A.C.,
            Tanner,H. and Lei,L.
  TITLE     Altered germline cyst formation and oogenesis in Tex14 mutant mice
  JOURNAL   Biol Open 10 (6) (2021)
   PUBMED   34156079
  REMARK    GeneRIF: Altered germline cyst formation and oogenesis in Tex14
            mutant mice.
REFERENCE   4  (bases 1 to 4776)
  AUTHORS   Scovell,J.M., Bournat,J.C., Szafran,A.T., Solis,M., Moore,J.,
            Rivera,A., Chen,C.H., Zhang,J., Wilken,N., Seth,A. and Jorgez,C.J.
  TITLE     PRSS50 is a testis protease responsible for proper sperm tail
            formation and function
  JOURNAL   Development 148 (8) (2021)
   PUBMED   33913480
REFERENCE   5  (bases 1 to 4776)
  AUTHORS   Soygur,B., Jaszczak,R.G., Fries,A., Nguyen,D.H., Malki,S., Hu,G.,
            Demir,N., Arora,R. and Laird,D.J.
  TITLE     Intercellular bridges coordinate the transition from pluripotency
            to meiosis in mouse fetal oocytes
  JOURNAL   Sci Adv 7 (15) (2021)
   PUBMED   33827806
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 4776)
  AUTHORS   Greenbaum,M.P., Ma,L. and Matzuk,M.M.
  TITLE     Conversion of midbodies into germ cell intercellular bridges
  JOURNAL   Dev Biol 305 (2), 389-396 (2007)
   PUBMED   17383626
REFERENCE   7  (bases 1 to 4776)
  AUTHORS   Greenbaum,M.P., Yan,W., Wu,M.H., Lin,Y.N., Agno,J.E., Sharma,M.,
            Braun,R.E., Rajkovic,A. and Matzuk,M.M.
  TITLE     TEX14 is essential for intercellular bridges and fertility in male
            mice
  JOURNAL   Proc Natl Acad Sci U S A 103 (13), 4982-4987 (2006)
   PUBMED   16549803
  REMARK    GeneRIF: TEX14 is required for intercellular bridges in vertebrate
            germ cells, and these studies provide evidence that the
            intercellular bridge is essential for spermatogenesis and fertility
REFERENCE   8  (bases 1 to 4776)
  AUTHORS   Wang,P.J., Page,D.C. and McCarrey,J.R.
  TITLE     Differential expression of sex-linked and autosomal
            germ-cell-specific genes during spermatogenesis in the mouse
  JOURNAL   Hum Mol Genet 14 (19), 2911-2918 (2005)
   PUBMED   16118233
  REMARK    GeneRIF: Tex14 is expressed throughout spermatogenesis
REFERENCE   9  (bases 1 to 4776)
  AUTHORS   Wu,M.H., Rajkovic,A., Burns,K.H., Yan,W., Lin,Y.N. and Matzuk,M.M.
  TITLE     Sequence and expression of testis-expressed gene 14 (Tex14): a gene
            encoding a protein kinase preferentially expressed during
            spermatogenesis
  JOURNAL   Gene Expr Patterns 3 (2), 231-236 (2003)
   PUBMED   12711554
  REMARK    GeneRIF: Predicted to be localized to the nucleus, suggesting a key
            role in regulating gene expression during mammalian
            spermatogenesis.
REFERENCE   10 (bases 1 to 4776)
  AUTHORS   Wang,P.J., McCarrey,J.R., Yang,F. and Page,D.C.
  TITLE     An abundance of X-linked genes expressed in spermatogonia
  JOURNAL   Nat Genet 27 (4), 422-426 (2001)
   PUBMED   11279525
  REMARK    GeneRIF: Tex14 is specifically expressed in testis in a cDNA
            subtraction screen
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BK000966.1 and AL669902.8.
            
            On Oct 25, 2007 this sequence version replaced NM_031386.1.
            
            Transcript Variant: This variant (2) uses a different first exon
            which results in the use of a downstream start codon, compared to
            variant 1. The resulting protein (isoform b) has a shorter
            N-terminus when it is compared to isoform a.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BK000966.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849384, SAMN01164134
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1076              BK000966.1         1-1076
            1077-1112           AL669902.8         111492-111527
            1113-4776           BK000966.1         1113-4776
FEATURES             Location/Qualifiers
     source          1..4776
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10090"
                     /chromosome="11"
                     /map="11 52.09 cM"
     gene            1..4776
                     /gene="Tex14"
                     /note="testis expressed gene 14 intercellular bridge
                     forming factor"
                     /db_xref="GeneID:83560"
                     /db_xref="MGI:MGI:1933227"
     exon            1..106
                     /gene="Tex14"
                     /inference="alignment:Splign:2.1.0"
     exon            107..243
                     /gene="Tex14"
                     /inference="alignment:Splign:2.1.0"
     CDS             108..4460
                     /gene="Tex14"
                     /note="isoform b is encoded by transcript variant 2;
                     testis-expressed protein 14; testis-expressed sequence 14
                     protein; inactive serine/threonine-protein kinase TEX14"
                     /codon_start=1
                     /product="inactive serine/threonine-protein kinase TEX14
                     isoform b"
                     /protein_id="NP_113563.2"
                     /db_xref="CCDS:CCDS48877.1"
                     /db_xref="GeneID:83560"
                     /db_xref="MGI:MGI:1933227"
                     /translation="MSRGAPFPVPCPVLLGTFTDDSLEAQLHEYAKQGNCVKLKKILK
                     KGVCVDAVNTQGQSALFVAALLGHVKLVDVLVDYGSDPNHRCFDGSTPVHAAAFSGNQ
                     WILSKLLTAGGDLRLHDEKGRNPQAWALTAGKDRSTQMVEFMQRCTSHMKAIIQGFSY
                     DLLKKIDSPQRLIGSPPWFGSLIQGSPNSSPNRQLKPGIISAQNIYSFGFGKFYLTSG
                     MQLTYPGSLPVIGEKEVVQADDEPTFSFFSGPYMVMTNLVWNRSRVTVKELNLPTRPH
                     CSRLRLADLLIAEQEHSSNLRHPNLLQLMAVCLSRDLEKIRLVYERITVGTLFSVLHE
                     RRSQFPVLHMEVIVHLLLQVADALIYLHSRGFIHRSLSSYAVHIVSAGEARLTNLEYL
                     TESQDSGAHRNVTRMPLPTQLYNWAAPEVVLQKAATVKSDIYSFSVIIQEILTDSIPW
                     NGLDGSLVKETIALGNYLEADVRLPEPYYDIVKSGIHAKQKNRTMNLQDIRYILKNDL
                     KEFIGAQKTQPTESPRGQSYEPHPDVNICLGLTSEYQKDPPDLDIKELKEMGSQPHSP
                     TDHSFLTVKPTLAPQTLDSSLSAQKPDNANVPSPPAACLAEEVRSPTASQDSLCSFEI
                     NEIYSGCLTLGTDKEEECLGTAASPEGDRPNQGDELPSLEEELDKMERELHCFCEEDK
                     SISEVDTDLLFEDDDWQSDSLGSLNLPEPTREAKGKTSSWSKTDEYVSKCVLNLKISQ
                     VMMQQSAEWLRKLEQEVEELEWAQKELDSQCSSLRDASLKFANAKFQPAVGPPSLAYL
                     PPVMQLPGLKQPENGGTWLTLARSPGNEREFQEGHFSKKPEKLSACGWKPFTQVSEES
                     RGDCSELNNQLPTLRGPGKQSTGEQLPSTQEARESLEKNTNQNSRSMASVSSEIYATK
                     SRNNEDNGEAHLKWRLAVKEMAEKAVSGQLLLPPWNPQSSAPFESKVENESTPLPRPP
                     IRGPESTEWQHILEYQRENDEPKGNTKFGKMDNSDCDKNKHSRWTGLQRFTGIRYPFF
                     RNHEQPEQNEASQASCDTSVGTEKFYSTSSPIGDDFERFQDSFAQRQGYVEENFQIRE
                     IFEKNAEILTKPQFQAIQCAEDKQDETLGETPKELKEKNTSLTDIQDLSSITYDQDGY
                     FKETSYKTPKLKHAPTSASTPLSPESISSAASHYEDCLENTTFHVKRGSTFCWNGQEA
                     MRTLSAKFTTVRERAKSLESLLASSKSLPAKLTDSKRLCMLSETGSSNVSAAFVTSTH
                     ATKRKSLPRELAEATSQQHLDELPPPAQELLDEIEQLKQQQVSSLASHENTARDLSVT
                     NKDKKHLEEQETNSSKDSSFLSSREIQDLEDTERAHSSLDEDLERFLQSPEENTALLD
                     PTKGSTREKKNKDQDVVEQKRKKKESIKPERRESDSSLGTLEEDELKPCFWKRLGWSE
                     PSRIIVLDQSDLSD"
     exon            244..358
                     /gene="Tex14"
                     /inference="alignment:Splign:2.1.0"
     exon            359..524
                     /gene="Tex14"
                     /inference="alignment:Splign:2.1.0"
     exon            525..661
                     /gene="Tex14"
                     /inference="alignment:Splign:2.1.0"
     exon            662..743
                     /gene="Tex14"
                     /inference="alignment:Splign:2.1.0"
     exon            744..874
                     /gene="Tex14"
                     /inference="alignment:Splign:2.1.0"
     exon            875..988
                     /gene="Tex14"
                     /inference="alignment:Splign:2.1.0"
     exon            989..1112
                     /gene="Tex14"
                     /inference="alignment:Splign:2.1.0"
     exon            1113..1291
                     /gene="Tex14"
                     /inference="alignment:Splign:2.1.0"
     exon            1292..1443
                     /gene="Tex14"
                     /inference="alignment:Splign:2.1.0"
     exon            1444..1634
                     /gene="Tex14"
                     /inference="alignment:Splign:2.1.0"
     exon            1635..1785
                     /gene="Tex14"
                     /inference="alignment:Splign:2.1.0"
     exon            1786..2705
                     /gene="Tex14"
                     /inference="alignment:Splign:2.1.0"
     exon            2706..2812
                     /gene="Tex14"
                     /inference="alignment:Splign:2.1.0"
     exon            2813..2895
                     /gene="Tex14"
                     /inference="alignment:Splign:2.1.0"
     exon            2896..3177
                     /gene="Tex14"
                     /inference="alignment:Splign:2.1.0"
     exon            3178..3278
                     /gene="Tex14"
                     /inference="alignment:Splign:2.1.0"
     exon            3279..3345
                     /gene="Tex14"
                     /inference="alignment:Splign:2.1.0"
     exon            3346..3427
                     /gene="Tex14"
                     /inference="alignment:Splign:2.1.0"
     exon            3428..3490
                     /gene="Tex14"
                     /inference="alignment:Splign:2.1.0"
     exon            3491..3618
                     /gene="Tex14"
                     /inference="alignment:Splign:2.1.0"
     exon            3619..3824
                     /gene="Tex14"
                     /inference="alignment:Splign:2.1.0"
     exon            3825..3921
                     /gene="Tex14"
                     /inference="alignment:Splign:2.1.0"
     exon            3922..3996
                     /gene="Tex14"
                     /inference="alignment:Splign:2.1.0"
     exon            3997..4074
                     /gene="Tex14"
                     /inference="alignment:Splign:2.1.0"
     exon            4075..4168
                     /gene="Tex14"
                     /inference="alignment:Splign:2.1.0"
     exon            4169..4261
                     /gene="Tex14"
                     /inference="alignment:Splign:2.1.0"
     exon            4262..4371
                     /gene="Tex14"
                     /inference="alignment:Splign:2.1.0"
     exon            4372..4423
                     /gene="Tex14"
                     /inference="alignment:Splign:2.1.0"
     exon            4424..4763
                     /gene="Tex14"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4739..4744
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Tex14"
                     /note="hexamer: AATAAA"
     polyA_site      4763
                     /gene="Tex14"
                     /note="major polyA site"
ORIGIN      
        1 acagcttccg gtgggtgacc ccgggactcc ggagctccct cagagtgtgg cggtttctga
       61 gcattcatgt cggcaggcct gcctagagta agaagtagct ggaaaacatg tctcgggggg
      121 ctccttttcc agtcccttgc ccggtgctgc tggggacctt cacagatgac tccctggagg
      181 ctcagcttca cgaatacgcc aagcaaggga actgcgtgaa gctcaagaag attcttaaga
      241 agggagtctg tgtcgatgca gttaacactc agggccagtc agcgctcttt gttgcagcat
      301 tactgggcca tgtgaaatta gtggatgttc tggtggatta tggatcagat ccaaatcacc
      361 gctgctttga cgggagcact cctgtccatg cagcagcctt ttcaggcaac cagtggatcc
      421 tcagtaaact gctgactgca gggggtgacc tgcgactcca tgatgagaag ggtcggaatc
      481 cacaggcctg ggccttgaca gcagggaagg atcgtagcac tcagatggtg gagttcatgc
      541 aacgttgtac ttcacatatg aaggctatca tccagggctt ctcctatgac cttctgaaga
      601 agatagactc tcctcagcga ctcatcggca gcccaccctg gtttggtagc ctcattcagg
      661 gaagcccaaa tagttctccc aaccgacagc ttaaacctgg aatcatttct gcccaaaata
      721 tctacagctt tggctttggg aagttttatc tcacctcagg gatgcagctt acttacccag
      781 gatctcttcc agtcattgga gagaaggaag tggtccaggc tgatgatgag cccaccttct
      841 ctttcttcag tggcccctac atggtcatga ctaacctcgt gtggaatagg agcagagtca
      901 cagtaaagga gctgaacctt cccacccgtc cccactgtag caggctgagg ttggccgact
      961 tgctgattgc tgagcaggag cacagcagca acctgcggca tcctaacctg ctgcaactga
     1021 tggctgtatg tttgtcccgg gacctggaga aaattcgcct ggtttacgag cgtatcacag
     1081 tcggcacact gttcagtgtc ctccatgaac gaaggtccca gttcccagtg ctgcacatgg
     1141 aggtgattgt gcacctgttg ctccaggttg ctgatgcctt gatatacctg cattcccggg
     1201 ggttcatcca ccgctccctc agctcctacg ctgtccacat cgtctctgca ggagaagcaa
     1261 ggctgactaa cctggaatac ctgacggaaa gccaggacag tggtgcacac aggaacgtga
     1321 ctcgaatgcc cctccccacc cagctgtaca actgggctgc accagaagtg gtcttgcaga
     1381 aggcagccac ggtgaagtca gacatataca gcttttccgt gatcatacaa gagatcttaa
     1441 cagacagtat accctggaat ggcttggatg gctcacttgt taaagaaacc atagccttgg
     1501 gaaattattt agaagctgat gtcaggcttc cggaacctta ctatgatatt gttaagtcag
     1561 gaatccatgc caagcagaag aaccgaacaa tgaaccttca agatattcgt tatattctga
     1621 agaatgactt aaaggaattt attggagctc agaaaactca gccaaccgag agccccagag
     1681 ggcagagcta tgaaccccat cctgatgtta atatctgcct aggtctaact tcagaatatc
     1741 aaaaggaccc tccagacttg gacatcaagg aactaaagga aatgggtagt cagccccatt
     1801 cacctacaga tcactccttt ctcactgtaa aaccaacact agctcctcag accctagatt
     1861 caagtctgtc agcccagaaa cctgacaatg caaatgttcc ttctcctcct gctgcatgtc
     1921 tggcagaaga ggtcaggagc cccactgcaa gtcaggacag cctctgcagc tttgaaatca
     1981 atgagatcta ctcaggctgc ttgacactgg gaactgacaa ggaggaagag tgtctgggga
     2041 ctgctgcttc acctgagggg gatagaccaa accagggaga tgagctgcca tccctggaag
     2101 aagagctcga taagatggag agagaattgc actgtttttg tgaagaggac aaaagcattt
     2161 cagaagttga cacagacctt ctttttgagg atgatgactg gcaaagtgat tctcttggtt
     2221 cactcaacct gcccgaacca accagagaag ccaagggcaa aacgagcagc tggtccaaga
     2281 ctgatgagta tgtcagtaag tgtgtgctga atctgaagat ttcacaggtg atgatgcagc
     2341 agagcgctga gtggctgagg aagcttgagc aggaggtaga ggagctcgag tgggcacaga
     2401 aggagctgga cagtcagtgc agcagtttgc gggatgcttc attaaagttt gcaaatgcca
     2461 agttccagcc ggctgtaggc cctccatctt tggcctatct tcctcctgtt atgcaattac
     2521 cagggctcaa gcagcctgaa aatggtggca cctggttaac cctagcaagg tctccaggaa
     2581 atgagagaga gttccaagag ggacatttta gcaaaaaacc tgagaaacta agtgcctgtg
     2641 gctggaagcc ttttacacaa gtgtctgaag aaagcagagg ggactgctca gagctaaaca
     2701 atcagctgcc gactcttcgt ggtcctggga agcagagcac aggtgagcag ttaccatcca
     2761 ctcaagaagc aagggagagt ttggaaaaaa atacaaacca aaatagtagg agtatggcgt
     2821 ctgtgtcttc tgaaatctat gctactaagt caagaaataa tgaggataat ggagaggcac
     2881 acttgaaatg gagattggca gtaaaagaaa tggcagagaa agcagtttcc ggacagctct
     2941 tattacctcc ttggaatcct cagagtagtg cgccttttga gagtaaggtt gaaaatgaga
     3001 gcactccttt gccacggccc ccaattagag gtcctgagag cacagaatgg cagcacattt
     3061 tagaatacca gagggaaaat gatgagccca aaggaaatac gaagtttggc aaaatggaca
     3121 acagtgactg tgacaagaac aagcacagca gatggacagg cctccagcgc ttcactggta
     3181 ttagataccc attcttcaga aaccacgagc agccagagca gaatgaagcc tctcaagcaa
     3241 gctgtgacac gtctgtgggc actgagaagt tctacagcac ctcaagtccc ataggagacg
     3301 actttgaaag attccaagat tcttttgccc aacgtcaagg ctatgttgaa gaaaatttcc
     3361 aaataagaga aatatttgaa aagaatgctg agattttgac caagcctcag tttcaagcta
     3421 ttcaatgtgc tgaagacaaa caagacgaaa cattagggga gacgccaaag gaactgaaag
     3481 agaaaaacac atcactgaca gacattcaag acttgtccag catcacctat gatcaagacg
     3541 gctattttaa ggaaacctca tacaaaacac ccaaattaaa acacgcacca actagtgcca
     3601 gtaccccgct aagcccagag tcgatttctt cagctgctag tcactatgaa gactgccttg
     3661 aaaataccac atttcatgtt aaaagaggat ctacattttg ttggaatggc caagaagcta
     3721 tgagaacttt gtctgccaaa tttacaactg tccgagagag agctaagagc ctggaatcac
     3781 ttctcgcttc ttctaaaagc ctacctgcca agctgactga ctccaagaga ttgtgtatgt
     3841 tgagtgagac tggctcttct aacgtttctg cggcatttgt aacatcaact catgctacca
     3901 agaggaagag cctacccaga gaactggcag aagccacctc tcaacagcat cttgatgagc
     3961 ttccaccacc agctcaggag ctacttgatg aaattgagca actgaagcag cagcaggtct
     4021 catccctggc gtcacatgag aacacggcac gtgatctgag tgtcactaac aaggataaga
     4081 agcatttgga agaacaagaa accaacagta gtaaagacag cagttttctt tccagcagag
     4141 aaattcagga tctggaagat acagagagag ctcattcttc tcttgatgag gacctggaaa
     4201 gattcctgca gtcacctgag gagaacacgg cactgctgga ccctaccaag ggctctacaa
     4261 gggagaaaaa aaacaaagat caagacgttg ttgagcagaa gagaaaaaag aaagaaagca
     4321 tcaagccaga gagaagggag tcagacagct ccctagggac cttggaagaa gatgaactaa
     4381 aaccctgttt ttggaagcga ctgggttggt ccgaaccttc caggataatt gtgctggatc
     4441 agagcgactt gtcagactga ttggaactgg accgtgcaag cattgtggct gtggcctcct
     4501 ttcttctctt atctgcttca gttgcctcaa ggacagtagt ttcagtctgt aactcacact
     4561 ttgttctgct gctactatgg gcacaataat gtgtccctat catgtgtagc atgcttaatc
     4621 atttgtttta aatcaggttt ctgaaaagtg caaagtaacc atagtggcaa acattttgtg
     4681 ttcagaatgg cttttgtttt ttctgatgta aatttttgaa accataactt tgttatataa
     4741 taaagtgttt actttcaatg ctaaaaaaaa aaaaaa
//
