LOCUS       NM_001039657            2234 bp    mRNA    linear   ROD 31-MAY-2024
DEFINITION  Mus musculus testis expressed metallothionein like (Tesmin),
            transcript variant 1, mRNA.
ACCESSION   NM_001039657 XM_001052048 XM_001052049 XM_001052050
VERSION     NM_001039657.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2234)
  AUTHORS   Oura,S., Ninomiya,A., Sugihara,F., Matzuk,M.M. and Ikawa,M.
  TITLE     Proximity-dependent biotin labeling in testicular germ cells
            identified TESMIN-associated proteins
  JOURNAL   Sci Rep 12 (1), 22198 (2022)
   PUBMED   36564444
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2234)
  AUTHORS   Huang,Y., Wu,Q. and Tan,X.
  TITLE     Metallothionein-like 5 expression is correlated with poor prognosis
            and promotes proliferation of cervical squamous cell carcinoma
  JOURNAL   Bioengineered 13 (3), 6955-6965 (2022)
   PUBMED   35249447
  REMARK    GeneRIF: Metallothionein-like 5 expression is correlated with poor
            prognosis and promotes proliferation of cervical squamous cell
            carcinoma.
REFERENCE   3  (bases 1 to 2234)
  AUTHORS   Zhang,X., Li,M., Jiang,X., Ma,H., Fan,S., Li,Y., Yu,C., Xu,J.,
            Khan,R., Jiang,H. and Shi,Q.
  TITLE     Nuclear translocation of MTL5 from cytoplasm requires its direct
            interaction with LIN9 and is essential for male meiosis and
            fertility
  JOURNAL   PLoS Genet 17 (8), e1009753 (2021)
   PUBMED   34388164
  REMARK    GeneRIF: Nuclear translocation of MTL5 from cytoplasm requires its
            direct interaction with LIN9 and is essential for male meiosis and
            fertility.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2234)
  AUTHORS   Oji,A., Isotani,A., Fujihara,Y., Castaneda,J.M., Oura,S. and
            Ikawa,M.
  TITLE     Tesmin, Metallothionein-Like 5, is Required for Spermatogenesis in
            Micedagger
  JOURNAL   Biol Reprod 102 (4), 975-983 (2020)
   PUBMED   31916570
  REMARK    GeneRIF: Tesmin, Metallothionein-Like 5, is Required for
            Spermatogenesis in Micedagger.
REFERENCE   5  (bases 1 to 2234)
  AUTHORS   Olesen,C., Moller,M. and Byskov,A.G.
  TITLE     Tesmin transcription is regulated differently during male and
            female meiosis
  JOURNAL   Mol Reprod Dev 67 (1), 116-126 (2004)
   PUBMED   14648882
  REMARK    GeneRIF: Data suggest that tesmin transcription may be controlled
            by two different mechanisms during male and female meiosis.
REFERENCE   6  (bases 1 to 2234)
  AUTHORS   Sutou,S., Miwa,K., Matsuura,T., Kawasaki,Y., Ohinata,Y. and
            Mitsui,Y.
  TITLE     Native tesmin is a 60-kilodalton protein that undergoes dynamic
            changes in its localization during spermatogenesis in mice
  JOURNAL   Biol Reprod 68 (5), 1861-1869 (2003)
   PUBMED   12606435
  REMARK    GeneRIF: tesmin is involved in multiple stages of spermatogenesis
            and spermiogenesis
REFERENCE   7  (bases 1 to 2234)
  AUTHORS   Matsuura,T., Kawasaki,Y., Miwa,K., Sutou,S., Ohinata,Y., Yoshida,F.
            and Mitsui,Y.
  TITLE     Germ cell-specific nucleocytoplasmic shuttling protein, tesmin,
            responsive to heavy metal stress in mouse testes
  JOURNAL   J Inorg Biochem 88 (2), 183-191 (2002)
   PUBMED   11803038
  REMARK    GeneRIF: regulation of the tesmin nucleocytoplasmic shuttling by
            the effect of metal stress
REFERENCE   8  (bases 1 to 2234)
  AUTHORS   Sugihara,T., Wadhwa,R., Kaul,S.C. and Mitsui,Y.
  TITLE     A novel testis-specific metallothionein-like protein, tesmin, is an
            early marker of male germ cell differentiation
  JOURNAL   Genomics 57 (1), 130-136 (1999)
   PUBMED   10191092
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK161511.1, AK015732.1 and AK015724.1.
            
            On Nov 8, 2013 this sequence version replaced NM_001039657.1.
            
            Transcript Variant: This variant (1) encodes the longest isoform
            (a).
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK161511.1, AB057423.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849381, SAMN00849384
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-54                AK161511.1         2-55
            55-55               AK015732.1         28-28
            56-1035             AK161511.1         57-1036
            1036-1036           AK015724.1         976-976
            1037-2234           AK161511.1         1038-2235
FEATURES             Location/Qualifiers
     source          1..2234
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="19"
                     /map="19 3.15 cM"
     gene            1..2234
                     /gene="Tesmin"
                     /gene_synonym="Mtl5"
                     /note="testis expressed metallothionein like"
                     /db_xref="GeneID:17771"
                     /db_xref="MGI:MGI:1340029"
     exon            1..477
                     /gene="Tesmin"
                     /gene_synonym="Mtl5"
                     /inference="alignment:Splign:2.1.0"
     CDS             94..1521
                     /gene="Tesmin"
                     /gene_synonym="Mtl5"
                     /note="isoform a is encoded by transcript variant 1;
                     testis-specific metallothionein-like protein;
                     metallothionein-like 5, testis-specific (tesmin)"
                     /codon_start=1
                     /product="tesmin isoform a"
                     /protein_id="NP_001034746.1"
                     /db_xref="CCDS:CCDS37880.1"
                     /db_xref="GeneID:17771"
                     /db_xref="MGI:MGI:1340029"
                     /translation="MEDALLGAMTGPEDELGAELFGSERVFADGLALSPAGGAADRDE
                     LPVLADAYLGATEPGEPLLRALSPPPGAEVPAALLGDFPGLPELRSPDDAAPPPAYSV
                     HVLSSLLPGARGPALLPLSAGVRVIPVEIKEAGGSVPGGSPEDAAFQAPLAQESCCKF
                     PSSQEAEEASSCPRKKDSSPMVICQLKGGAQMLCIDNCGARELKALHLLPQYDDQSSF
                     PQSELPKPMTTLVGRLLPVPAKLNLITQVDNGALPSAVNGAAFPSGPALQGPPKITLS
                     GYCDCFSSGDFCNSCSCNNLRHELERFKAIKACLDRNPEAFQPKMGKGRLGAAKLRHS
                     KGCNCKRSGCLKNYCECYEAKIMCSSICKCIACKNYEESPERKMLMSTPHYMEPGDFE
                     SSHYLSPAKFSGPPKLRKNRQAFSCISWEVVEATCACLLAQGEEAEQEHCSPSLAEQM
                     ILEEFGRCLSQILHIEFKSKGLKIE"
     exon            478..636
                     /gene="Tesmin"
                     /gene_synonym="Mtl5"
                     /inference="alignment:Splign:2.1.0"
     exon            637..757
                     /gene="Tesmin"
                     /gene_synonym="Mtl5"
                     /inference="alignment:Splign:2.1.0"
     exon            758..831
                     /gene="Tesmin"
                     /gene_synonym="Mtl5"
                     /inference="alignment:Splign:2.1.0"
     exon            832..923
                     /gene="Tesmin"
                     /gene_synonym="Mtl5"
                     /inference="alignment:Splign:2.1.0"
     exon            924..1014
                     /gene="Tesmin"
                     /gene_synonym="Mtl5"
                     /inference="alignment:Splign:2.1.0"
     exon            1015..1152
                     /gene="Tesmin"
                     /gene_synonym="Mtl5"
                     /inference="alignment:Splign:2.1.0"
     exon            1153..1328
                     /gene="Tesmin"
                     /gene_synonym="Mtl5"
                     /inference="alignment:Splign:2.1.0"
     exon            1329..2234
                     /gene="Tesmin"
                     /gene_synonym="Mtl5"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1770..1775
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Tesmin"
                     /gene_synonym="Mtl5"
                     /note="hexamer: CATAAA"
     polyA_site      1792
                     /gene="Tesmin"
                     /gene_synonym="Mtl5"
                     /note="major polyA site"
     regulatory      2175..2180
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Tesmin"
                     /gene_synonym="Mtl5"
                     /note="hexamer: AATAAA"
     polyA_site      2196
                     /gene="Tesmin"
                     /gene_synonym="Mtl5"
                     /note="major polyA site"
ORIGIN      
        1 ccctttccct aacggtcaac ggtcgtccag cctctcagaa gcaaggcgag ctggacggcc
       61 gcgtcgtgtc gctgttctcg ggtcccagtg gccatggagg acgcgctgct cggcgccatg
      121 actggccccg aagacgagct gggcgccgag ctgttcgggt cagagcgtgt gttcgcggac
      181 gggctggcgc tgagccccgc aggcggtgcg gccgatcggg atgagttgcc ggttctggcc
      241 gacgcctacc tgggcgccac cgagccaggg gagccgctgc tgcgtgcgct cagccccccg
      301 ccgggcgctg aggtaccggc cgccctgctc ggcgacttcc cggggctgcc cgagctccgc
      361 agcccagacg acgccgctcc gcctcccgcc tacagcgtgc acgtgttatc gtcgttactt
      421 cccggtgctc gcgggcccgc gctgttgccg ctaagcgcag gagtgcgcgt gatcccagtt
      481 gaaatcaaag aagcaggtgg tagtgtgcca ggcggcagcc ctgaagacgc agctttccag
      541 gcccctctgg ctcaggaatc ctgttgcaag ttcccatcat cccaggaggc agaggaggcc
      601 tccagctgcc ctcggaagaa agactccagc cccatggtga tttgtcagct gaaaggaggc
      661 gcccagatgc tctgcataga caactgtggc gcgagggagc tcaaagcgct ccatctgctt
      721 cctcagtacg atgaccagag cagtttccct cagtcagagc tccctaagcc aatgacaact
      781 ttagtgggaa gacttctgcc agtaccagcg aagttaaatc tcatcacaca ggttgataat
      841 ggagctctcc catcagctgt caatggggct gcctttccct ctggacctgc tctgcaaggg
      901 ccacccaaaa taactctgtc tgggtactgt gactgcttct ccagcgggga cttctgcaac
      961 agctgcagct gcaacaacct gcgccatgag ctcgagcgct tcaaagccat aaaggcgtgt
     1021 cttgatagaa atcctgaagc tttccaacca aaaatgggga aaggccgtct gggagctgct
     1081 aaacttcgac acagcaaagg gtgcaactgt aagcgctcag gctgcctgaa gaactactgt
     1141 gagtgctatg aggccaaaat catgtgttct tccatttgca aatgcattgc ttgcaaaaac
     1201 tatgaagaaa gtccagaacg aaaaatgctg atgagcacac cccactacat ggagcctggg
     1261 gactttgaga gcagccatta tttgtcccca gccaagttct caggacctcc aaaactgaga
     1321 aaaaataggc aggccttctc ctgtatctcc tgggaagtag tggaggccac atgtgcctgc
     1381 ctgctggccc agggtgagga agcagagcag gagcactgtt ccccaagctt ggctgagcag
     1441 atgatcctgg aggagtttgg aaggtgcctg tcgcagattc tccacatcga gttcaagtcc
     1501 aaggggctga aaattgagta gcgtgcaagc tggtaaaggg gaatgcctgt ggcaagcctc
     1561 agccctggga atctgcaccg aggaagctgg tgcccaggga ggagcagagg ccgcgcatca
     1621 tggccaggtc agctgtgagg tctgagtgat ctgcatggta ctggccagcc tactcaaggt
     1681 atcctaaagt gcaagcaggc agagccaccc tggggatgga cactggccct cctgtccctg
     1741 gggaggccct ctggggactc cctgccctgc ataaaaagag ggtgattttc tacttgttgt
     1801 tatgtgtttg ctttcaaatt gcttagtagt acctccattc aagttattat gagccagcct
     1861 caagttagag agctaggctc ttcttcaggt ggactctgcc caaatcacat acaagtcagg
     1921 tggccatcag gggtcttcca ggccaggcct gtgacaggag atatgggagg ggggtcgggt
     1981 tagagctggg tttgtttgga ttttttgcgt ttttttcttc ctgtatttct gcttgaagtg
     2041 agaaaacttg tctcctgtcc aaccctcttc tccataatta ctgctgcacg gtcgcctgct
     2101 gaccagtcac agtgacctca gacaccagaa ggtgaggtgg cttattatgc ccacactttg
     2161 tgttttgttg tgagaataaa cctttccaga ctcccagttg gttagtagct gaaaatatat
     2221 ttgtctcagt accc
//
