LOCUS       NM_207176               2442 bp    mRNA    linear   ROD 09-FEB-2025
DEFINITION  Mus musculus testin LIM domain protein (Tes), mRNA.
ACCESSION   NM_207176
VERSION     NM_207176.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2442)
  AUTHORS   Bedogni,F. and Hevner,R.F.
  TITLE     Cell-Type-Specific Gene Expression in Developing Mouse Neocortex:
            Intermediate Progenitors Implicated in Axon Development
  JOURNAL   Front Mol Neurosci 14, 686034 (2021)
   PUBMED   34321999
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2442)
  AUTHORS   Weeks,R.J., Ludgate,J.L., Le Mee,G., Khanal,R., Mehta,S.,
            Williams,G., Slatter,T.L., Braithwaite,A.W. and Morison,I.M.
  TITLE     Silencing of Testin expression is a frequent event in spontaneous
            lymphomas from Trp53-mutant mice
  JOURNAL   Sci Rep 10 (1), 16255 (2020)
   PUBMED   33004921
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2442)
  AUTHORS   Gao,L., Liu,Y., Guo,S., Xiao,L., Liang,C. and Wang,X.
  TITLE     Testin protects against cardiac hypertrophy by targeting a
            calcineurin-dependent signalling pathway
  JOURNAL   J Cell Mol Med 23 (1), 328-339 (2019)
   PUBMED   30467953
  REMARK    GeneRIF: Expression of testin is downregulated in murine
            hypertrophic hearts.Testin serves as a crucial regulator of the
            hypertrophic reaction by hindering the calcineurin-dependent
            pathway in the heart.
REFERENCE   4  (bases 1 to 2442)
  AUTHORS   Peng,G., Suo,S., Chen,J., Chen,W., Liu,C., Yu,F., Wang,R., Chen,S.,
            Sun,N., Cui,G., Song,L., Tam,P.P., Han,J.D. and Jing,N.
  TITLE     Spatial Transcriptome for the Molecular Annotation of Lineage Fates
            and Cell Identity in Mid-gastrula Mouse Embryo
  JOURNAL   Dev Cell 36 (6), 681-697 (2016)
   PUBMED   27003939
  REMARK    Erratum:[Dev Cell. 2020 Dec 21;55(6):802-804. doi:
            10.1016/j.devcel.2020.11.018.. PMID: 33352143]
REFERENCE   5  (bases 1 to 2442)
  AUTHORS   Hadzic,E., Catillon,M., Halavatyi,A., Medves,S., Van Troys,M.,
            Moes,M., Baird,M.A., Davidson,M.W., Schaffner-Reckinger,E., Ampe,C.
            and Friederich,E.
  TITLE     Delineating the Tes Interaction Site in Zyxin and Studying Cellular
            Effects of Its Disruption
  JOURNAL   PLoS One 10 (10), e0140511 (2015)
   PUBMED   26509500
  REMARK    GeneRIF: zyxin influences focal adhesion dynamics, that it recruits
            Tes and that this interaction is functional in regulating cell
            spreading.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 2442)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
REFERENCE   7  (bases 1 to 2442)
  AUTHORS   Bekman,E. and Henrique,D.
  TITLE     Embryonic expression of three mouse genes with homology to the
            Drosophila melanogaster prickle gene
  JOURNAL   Mech Dev 119 Suppl 1, S77-S81 (2002)
   PUBMED   14516664
REFERENCE   8  (bases 1 to 2442)
  AUTHORS   Tatarelli,C., Linnenbach,A., Mimori,K. and Croce,C.M.
  TITLE     Characterization of the human TESTIN gene localized in the FRA7G
            region at 7q31.2
  JOURNAL   Genomics 68 (1), 1-12 (2000)
   PUBMED   10950921
REFERENCE   9  (bases 1 to 2442)
  AUTHORS   Divecha,N. and Charleston,B.
  TITLE     Cloning and characterisation of two new cDNAs encoding murine
            triple LIM domains
  JOURNAL   Gene 156 (2), 283-286 (1995)
   PUBMED   7758969
REFERENCE   10 (bases 1 to 2442)
  AUTHORS   Porteus,M.H., Brice,A.E., Bulfone,A., Usdin,T.B., Ciaranello,R.D.
            and Rubenstein,J.L.
  TITLE     Isolation and characterization of a library of cDNA clones that are
            preferentially expressed in the embryonic telencephalon
  JOURNAL   Brain Res Mol Brain Res 12 (1-3), 7-22 (1992)
   PUBMED   1372074
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC159332.11.
            
            On Mar 20, 2007 this sequence version replaced NM_207176.2.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK132249.1, AK167274.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-165               AC159332.11        109854-110018       c
            166-251             AC159332.11        88945-89030         c
            252-504             AC159332.11        78787-79039         c
            505-834             AC159332.11        77577-77906         c
            835-1050            AC159332.11        75248-75463         c
            1051-1209           AC159332.11        74727-74885         c
            1210-2442           AC159332.11        69342-70574         c
FEATURES             Location/Qualifiers
     source          1..2442
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10090"
                     /chromosome="6"
                     /map="6 7.67 cM"
     gene            1..2442
                     /gene="Tes"
                     /gene_synonym="D6Ertd352e; Tes1; Tes2; TESS; testin2"
                     /note="testin LIM domain protein"
                     /db_xref="GeneID:21753"
                     /db_xref="MGI:MGI:105081"
     exon            1..165
                     /gene="Tes"
                     /gene_synonym="D6Ertd352e; Tes1; Tes2; TESS; testin2"
                     /inference="alignment:Splign:2.1.0"
     CDS             139..1398
                     /gene="Tes"
                     /gene_synonym="D6Ertd352e; Tes1; Tes2; TESS; testin2"
                     /note="testin 2; TES1/TES2; testis derived transcript"
                     /codon_start=1
                     /product="testin"
                     /protein_id="NP_997059.1"
                     /db_xref="CCDS:CCDS39429.1"
                     /db_xref="GeneID:21753"
                     /db_xref="MGI:MGI:105081"
                     /translation="MDLETKMKKMGLGHEQGFGAPCLKCKENCEGFELHFWRKICRNC
                     KCGQEEHDVLLSNEEDRKVGRLFEDTKYTTLIAKLKSDGIPMYKRNVMILTNPVAAKK
                     NVSINTVTYEWAPPVQNQALARQYMQMLPKEKQPVAGSEGAQYRKKQLAKQLPAHDQD
                     PSKCHELSPKEVKEMEQFVKKYKSEALGVGDVKFPSEMNAQGDKVHNPAGNRHAPAAV
                     ASKDKSAESKKTQYSCYCCKHTMNEGEPAIYAERAGYDKLWHPACFICSTCGELLVDM
                     IYFWKNGKLYCGRHYCDSEKPRCAGCDELIFSNEYTQAENQNWHLKHFCCFDCDHILA
                     GKIYVMVTDKPVCKPCYVKNHAVVCQGCHNAIDPEVQRVTYNNFSWHASTECFLCSCC
                     SKCLIGQKFMPVEGMVFCSVECKRMMS"
     exon            166..251
                     /gene="Tes"
                     /gene_synonym="D6Ertd352e; Tes1; Tes2; TESS; testin2"
                     /inference="alignment:Splign:2.1.0"
     exon            252..504
                     /gene="Tes"
                     /gene_synonym="D6Ertd352e; Tes1; Tes2; TESS; testin2"
                     /inference="alignment:Splign:2.1.0"
     exon            505..834
                     /gene="Tes"
                     /gene_synonym="D6Ertd352e; Tes1; Tes2; TESS; testin2"
                     /inference="alignment:Splign:2.1.0"
     exon            835..1050
                     /gene="Tes"
                     /gene_synonym="D6Ertd352e; Tes1; Tes2; TESS; testin2"
                     /inference="alignment:Splign:2.1.0"
     exon            1051..1209
                     /gene="Tes"
                     /gene_synonym="D6Ertd352e; Tes1; Tes2; TESS; testin2"
                     /inference="alignment:Splign:2.1.0"
     exon            1210..2442
                     /gene="Tes"
                     /gene_synonym="D6Ertd352e; Tes1; Tes2; TESS; testin2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2421..2426
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Tes"
                     /gene_synonym="D6Ertd352e; Tes1; Tes2; TESS; testin2"
                     /note="hexamer: AATAAA"
     polyA_site      2442
                     /gene="Tes"
                     /gene_synonym="D6Ertd352e; Tes1; Tes2; TESS; testin2"
                     /note="major polyA site"
ORIGIN      
        1 ctctttgttc caggtcccag ccgctgccga accgggctgc ggaagttcga gtgcccatgc
       61 tgagtagcag cggcagcggc gccggagttc ccgcagcgga gatcgcggac gcgggactga
      121 gcccctccga cgcccagtat ggacctggaa accaaaatga agaagatggg cttaggtcat
      181 gagcaaggat ttggagcccc ctgtctaaaa tgcaaagaga actgtgaagg atttgaacta
      241 cacttctgga ggaaaatatg tcgtaactgc aaatgtggtc aagaagagca tgacgtcctc
      301 ctgagcaatg aggaggaccg gaaagtgggg agactctttg aggacactaa gtacaccacc
      361 ctgatcgcca agctgaagtc agatggcatt cccatgtaca aacgcaacgt tatgatcttg
      421 accaatccag tggctgccaa gaagaacgtc tccatcaaca cagttaccta tgagtgggct
      481 cccccagttc aaaaccaggc tctggccagg cagtacatgc agatgctgcc taaggagaag
      541 cagccagtgg caggttcaga gggggcacag taccggaaga agcagctggc gaaacagctc
      601 ccggctcatg accaggatcc ttctaagtgc catgagttgt ctcccaaaga ggtgaaggag
      661 atggagcagt ttgtgaagaa atacaagagt gaggccctgg gcgtgggtga cgtgaagttt
      721 ccctctgaga tgaatgctca gggtgacaag gtgcacaacc ctgcggggaa cagacacgcc
      781 ccagcagcag tggcttccaa ggacaagtct gcagaatcca agaagaccca gtattcctgt
      841 tactgctgca aacacaccat gaacgaaggc gagccggcca tctatgctga aagggctggc
      901 tacgataagc tctggcaccc ggcgtgtttt atctgcagca cctgtggtga actcctggtc
      961 gacatgattt acttctggaa gaatgggaag ctgtactgtg gcagacatta ctgtgacagt
     1021 gagaagcccc gctgtgctgg ctgtgacgag ctgatattca gcaatgaata tacccaagca
     1081 gaaaaccaga attggcacct gaagcacttc tgctgctttg actgcgacca catcctagca
     1141 gggaaaatct acgtgatggt cactgacaag cctgtgtgca agccctgcta tgtgaagaac
     1201 catgctgtgg tgtgtcaagg atgccacaat gccatcgacc cggaagtgca gcgagtaacg
     1261 tacaacaact tcagctggca cgcatccaca gagtgtttcc tgtgctcttg ctgcagcaag
     1321 tgtcttatcg ggcagaagtt catgcccgta gaagggatgg ttttctgttc cgtggagtgt
     1381 aagaggatga tgtcctaaga ggagtgtacc aagccgtatg gagccatagc tgtccacagt
     1441 ggcctgcatt tcatgataaa ctgcagtttg aaaaaaaaaa taaaaggaaa aaaaccctac
     1501 ataataaacc agaatatttt gtctaatatt tttttctttt taaccttatg aattttgtct
     1561 gtcttaattt taaatgactt ttcatgattt aaaaaaaaac agtaaagaat tgtagctttt
     1621 atttctatac attaattaaa tctttgaatt ggaagcttga atttaatcag tccttataac
     1681 tgcatactcc tagtgccaaa tttatattaa gtgatagata tgaaatcact taaaattagt
     1741 gtcctgggag agatgaccat tttaacttct atgcttattt cacatgggaa atgtaaagga
     1801 agttgcatgt tgcctgacat gacgctgtgc acatagcctg cctcatcgat ggatgacaag
     1861 gtgacatcag ctcggtgaat ccttctggca gacagcgtgg tccacgatgg gcgttccttt
     1921 gcctaagcct ttgcatcccc tggtgaggca gaggggcctc tctcactgca tctctctctc
     1981 tgtttacctc acctccccat gttcgctttt gcatctagtg accgccatct gtggccttgc
     2041 atattttcta tgtgtgtggc tggaacaata tgtgcacttt gctcttagag attattattt
     2101 tttgcccaga acataaaagt aggccattca tttagttatt tttttttgaa acccaaagaa
     2161 tatgtatagg ttttggtacc cagggaattg taacttgtgc taatatcacc tctctgccat
     2221 gccttgactc aattttgcat gatttatggt gttaccagat ggatcctctc ttgtgctacc
     2281 tctgagcttg ctgtagacac aaaggctatt ttctttgcct gtgcactccc atccttgggt
     2341 gtttttaatg ttttcccttg tatgcattca tacataacta tgcactataa atattaaatg
     2401 gaatgactta tgttattgaa aataaagtct ctttcacttt aa
//
