LOCUS       NM_027951               1547 bp    mRNA    linear   ROD 22-SEP-2024
DEFINITION  Mus musculus tektin 4 (Tekt4), transcript variant 1, mRNA.
ACCESSION   NM_027951 XM_128462 XM_914777 XM_988695
VERSION     NM_027951.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1547)
  AUTHORS   Tai,L., Yin,G., Huang,X., Sun,F. and Zhu,Y.
  TITLE     In-cell structural insight into the stability of sperm microtubule
            doublet
  JOURNAL   Cell Discov 9 (1), 116 (2023)
   PUBMED   37989994
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1547)
  AUTHORS   Chen,Z., Shiozaki,M., Haas,K.M., Skinner,W.M., Zhao,S., Guo,C.,
            Polacco,B.J., Yu,Z., Krogan,N.J., Lishko,P.V., Kaake,R.M.,
            Vale,R.D. and Agard,D.A.
  TITLE     De novo protein identification in mammalian sperm using in situ
            cryoelectron tomography and AlphaFold2 docking
  JOURNAL   Cell 186 (23), 5041-5053 (2023)
   PUBMED   37865089
REFERENCE   3  (bases 1 to 1547)
  AUTHORS   Zhou,L., Liu,H., Liu,S., Yang,X., Dong,Y., Pan,Y., Xiao,Z.,
            Zheng,B., Sun,Y., Huang,P., Zhang,X., Hu,J., Sun,R., Feng,S.,
            Zhu,Y., Liu,M., Gui,M. and Wu,J.
  TITLE     Structures of sperm flagellar doublet microtubules expand the
            genetic spectrum of male infertility
  JOURNAL   Cell 186 (13), 2897-2910 (2023)
   PUBMED   37295417
REFERENCE   4  (bases 1 to 1547)
  AUTHORS   Roy,A., Lin,Y.N., Agno,J.E., DeMayo,F.J. and Matzuk,M.M.
  TITLE     Tektin 3 is required for progressive sperm motility in mice
  JOURNAL   Mol Reprod Dev 76 (5), 453-459 (2009)
   PUBMED   18951373
REFERENCE   5  (bases 1 to 1547)
  AUTHORS   Guan,J. and Yuan,L.
  TITLE     A heat-shock protein 40, DNAJB13, is an axoneme-associated
            component in mouse spermatozoa
  JOURNAL   Mol Reprod Dev 75 (9), 1379-1386 (2008)
   PUBMED   18247331
REFERENCE   6  (bases 1 to 1547)
  AUTHORS   Roy,A., Lin,Y.N., Agno,J.E., DeMayo,F.J. and Matzuk,M.M.
  TITLE     Absence of tektin 4 causes asthenozoospermia and subfertility in
            male mice
  JOURNAL   FASEB J 21 (4), 1013-1025 (2007)
   PUBMED   17244819
  REMARK    GeneRIF: TEKT4 is necessary for the proper coordinated beating of
            the sperm flagellum and male reproductive physiology
REFERENCE   7  (bases 1 to 1547)
  AUTHORS   Iida,H., Honda,Y., Matsuyama,T., Shibata,Y. and Inai,T.
  TITLE     Tektin 4 is located on outer dense fibers, not associated with
            axonemal tubulins of flagella in rodent spermatozoa
  JOURNAL   Mol Reprod Dev 73 (7), 929-936 (2006)
   PUBMED   16596631
  REMARK    GeneRIF: Data suggest that Tektin 4 is not associated with axonemal
            tubulins but instead associated with Outer Dense Fibers in rodent
            spermatozoa.
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC131323.10.
            
            On Mar 23, 2023 this sequence version replaced NM_027951.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AY485267.1, AK005842.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849381
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-675               AC131323.10        145580-146254
            676-746             AC131323.10        147735-147805
            747-890             AC131323.10        148115-148258
            891-1113            AC131323.10        148639-148861
            1114-1268           AC131323.10        149558-149712
            1269-1547           AC131323.10        150321-150599
FEATURES             Location/Qualifiers
     source          1..1547
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="17"
                     /map="17 12.6 cM"
     gene            1..1547
                     /gene="Tekt4"
                     /gene_synonym="1700010L19Rik"
                     /note="tektin 4"
                     /db_xref="GeneID:71840"
                     /db_xref="MGI:MGI:1919090"
     exon            1..675
                     /gene="Tekt4"
                     /gene_synonym="1700010L19Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             142..1485
                     /gene="Tekt4"
                     /gene_synonym="1700010L19Rik"
                     /note="isoform 1 is encoded by transcript variant 1;
                     testicular microtubules-related protein 4"
                     /codon_start=1
                     /product="tektin-4 isoform 1"
                     /protein_id="NP_082227.1"
                     /db_xref="CCDS:CCDS28519.1"
                     /db_xref="GeneID:71840"
                     /db_xref="MGI:MGI:1919090"
                     /translation="MAQTGVLLTKEPAPQSIDVCELPRKEYEVACNTGAYTSSGLATA
                     GFRTAKYLRDEWFQNSYARYHQAFADRDYSERQRHESGQLVAETGALAQRTQLDSTRK
                     VGERLEDMHCWKSELQREIDELSSETDLMMAQKLRLQRALDATSVPYSIATDNLQCRE
                     RRQHPDLVRDYVEVELLKETELIRNIQELLKRTIGQAVDQIRLNREHKESCEMNWSDK
                     VEVYNIDDTCSRYTNESTQVQFYPHSSKFEESASTPETWAKFNHDNLLRAERERLASV
                     NLRKLIDCILRDTAEDLRLQCDAVNSAFSSRCQELDDSLQKLQYHLRKTLTEITDQEH
                     QIAALKQAIKDKEAPLRVAQTRLYQRSHRPNVELCRDNAQFRLLSEVEELNMSLRALK
                     EKLQDAEQALRNLEDSRMSLEKDIAVKTNSLFIDRQKCMTHRNRYPSVLQLAGYQ"
     exon            676..746
                     /gene="Tekt4"
                     /gene_synonym="1700010L19Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            747..890
                     /gene="Tekt4"
                     /gene_synonym="1700010L19Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            891..1113
                     /gene="Tekt4"
                     /gene_synonym="1700010L19Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1114..1268
                     /gene="Tekt4"
                     /gene_synonym="1700010L19Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1269..1547
                     /gene="Tekt4"
                     /gene_synonym="1700010L19Rik"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1520..1525
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Tekt4"
                     /gene_synonym="1700010L19Rik"
                     /note="hexamer: AATAAA"
     polyA_site      1547
                     /gene="Tekt4"
                     /gene_synonym="1700010L19Rik"
                     /note="major polyA site"
ORIGIN      
        1 gtatacaatg ttccttaggc aaccaggaac cttgcttggg acagagtgac cagtactgct
       61 ccattgatcg tgcctggaca attgcccagt gaccctctct tcattccgcc tcccttcctg
      121 cttagccagg cagtgggtac catggcccaa acgggcgtgc tcctgaccaa ggagccggcc
      181 ccacagtcaa tagatgtctg tgagcttccg cgcaaagaat atgaagtggc ctgcaatact
      241 ggcgcctaca cgtcctcggg tctggccacc gctggcttcc gcacagccaa atacctgagg
      301 gacgagtggt ttcagaactc ttatgcccgc taccaccagg ccttcgcaga ccgggactac
      361 tctgagcggc agcggcatga gagcgggcag ctggtagccg agactggggc actggcccag
      421 cgcacacaac ttgactccac aagaaaggtg ggagagcgcc tggaggacat gcattgctgg
      481 aagtcagagc tgcagcgaga gatagacgag ctgtcttccg agacggacct gatgatggcc
      541 cagaagctgc ggctacagcg cgccttggat gccacctccg tgccttactc cattgctact
      601 gacaacctgc agtgcagaga acgccgccag cacccagacc ttgtccggga ctatgtggag
      661 gtggagctac tgaaggaaac cgagctgatc aggaacatcc aggaactcct gaagaggacg
      721 ataggacaag ccgtggacca gatacggctg aacagggagc ataaggagtc gtgtgagatg
      781 aactggtcgg acaaagtgga agtctacaac attgacgata cctgctcccg ctacaccaat
      841 gagagcaccc aggtgcagtt ctatccgcat tccagcaagt tcgaggagag tgcttccacg
      901 cccgagacgt gggccaagtt caatcacgac aacctgcttc gagccgagcg cgagcggcta
      961 gcctcggtta acctgcggaa gttaattgac tgcatcttgc gggatacggc cgaggacctg
     1021 cggctacagt gcgatgcagt gaactcggct ttctcaagcc gctgccaaga gctggacgac
     1081 tcactccaga agctgcagta ccaccttcga aagaccctga cggagatcac agaccaggaa
     1141 caccagattg cagccttgaa gcaggccatc aaggacaagg aggcccctct gcgtgtggcc
     1201 cagactcgcc tttaccagcg gtcacaccgg cccaatgtgg agttgtgccg ggacaatgcc
     1261 cagttcaggt tgctgagcga ggtggaggag ttgaacatgt ccctcagagc gttaaaggag
     1321 aagcttcagg atgcggagca ggcactgcgg aacctggagg attcacgcat gagtctggag
     1381 aaggacatcg cagtcaagac caacagcctc tttatcgacc gccagaagtg tatgacccac
     1441 cgcaaccgct accccagtgt cctccagctg gctggctacc agtgactggg gctggcacct
     1501 ggcctcactg cttcattcaa ataaagagct ctttagtttt ccgccta
//
