LOCUS       NM_021480               1900 bp    mRNA    linear   ROD 03-JUN-2024
DEFINITION  Mus musculus L-threonine dehydrogenase (Tdh), mRNA; nuclear gene
            for mitochondrial product.
ACCESSION   NM_021480
VERSION     NM_021480.5
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1900)
  AUTHORS   Nacarino-Palma,A., Gonzalez-Rico,F.J., Rejano-Gordillo,C.M.,
            Ordiales-Talavero,A., Merino,J.M. and Fernandez-Salguero,P.M.
  TITLE     The aryl hydrocarbon receptor promotes differentiation during mouse
            preimplantational embryo development
  JOURNAL   Stem Cell Reports 16 (9), 2351-2363 (2021)
   PUBMED   34478649
REFERENCE   2  (bases 1 to 1900)
  AUTHORS   Donato,V., Bonora,M., Simoneschi,D., Sartini,D., Kudo,Y., Saraf,A.,
            Florens,L., Washburn,M.P., Stadtfeld,M., Pinton,P. and Pagano,M.
  TITLE     The TDH-GCN5L1-Fbxo15-KBP axis limits mitochondrial biogenesis in
            mouse embryonic stem cells
  JOURNAL   Nat Cell Biol 19 (4), 341-351 (2017)
   PUBMED   28319092
  REMARK    GeneRIF: TDH, responsible for mitochondrial production of
            acetyl-CoA in mouse embryonic stem cells, and the acetyltransferase
            GCN5L1 cooperate to acetylate Lys501 in KBP, allowing its
            recognition by and degradation via Fbxo15, an F-box protein
            transcriptionally controlled by the pluripotency core factors and
            repressed following differentiation.
REFERENCE   3  (bases 1 to 1900)
  AUTHORS   He,C., Huang,X., Liu,Y., Li,F., Yang,Y., Tao,H., Han,C., Zhao,C.,
            Xiao,Y. and Shi,Y.
  TITLE     Structural insights on mouse L-threonine dehydrogenase: A
            regulatory role of Arg180 in catalysis
  JOURNAL   J Struct Biol 192 (3), 510-518 (2015)
   PUBMED   26492815
REFERENCE   4  (bases 1 to 1900)
  AUTHORS   Alexander,P.B., Wang,J. and McKnight,S.L.
  TITLE     Targeted killing of a mammalian cell based upon its specialized
            metabolic state
  JOURNAL   Proc Natl Acad Sci U S A 108 (38), 15828-15833 (2011)
   PUBMED   21896756
  REMARK    GeneRIF: Data shsow that metabolic flux through the TDH pathway is
            required for the viability and self-renewal of ES cells.
REFERENCE   5  (bases 1 to 1900)
  AUTHORS   Hou,J., Charters,A.M., Lee,S.C., Zhao,Y., Wu,M.K., Jones,S.J.,
            Marra,M.A. and Hoodless,P.A.
  TITLE     A systematic screen for genes expressed in definitive endoderm by
            Serial Analysis of Gene Expression (SAGE)
  JOURNAL   BMC Dev Biol 7, 92 (2007)
   PUBMED   17683524
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 1900)
  AUTHORS   Edgar,A.J.
  TITLE     Mice have a transcribed L-threonine aldolase/GLY1 gene, but the
            human GLY1 gene is a non-processed pseudogene
  JOURNAL   BMC Genomics 6, 32 (2005)
   PUBMED   15757516
  REMARK    Publication Status: Online-Only
REFERENCE   7  (bases 1 to 1900)
  AUTHORS   Cobellis,G., Nicolaus,G., Iovino,M., Romito,A., Marra,E.,
            Barbarisi,M., Sardiello,M., Di Giorgio,F.P., Iovino,N., Zollo,M.,
            Ballabio,A. and Cortese,R.
  TITLE     Tagging genes with cassette-exchange sites
  JOURNAL   Nucleic Acids Res 33 (4), e44 (2005)
   PUBMED   15741177
  REMARK    Publication Status: Online-Only
REFERENCE   8  (bases 1 to 1900)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
REFERENCE   9  (bases 1 to 1900)
  AUTHORS   Edgar,A.J.
  TITLE     Molecular cloning and tissue distribution of mammalian L-threonine
            3-dehydrogenases
  JOURNAL   BMC Biochem 3, 19 (2002)
   PUBMED   12097150
  REMARK    Publication Status: Online-Only
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BC058860.1 and AK077571.1.
            
            On Jul 18, 2009 this sequence version replaced NM_021480.4.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC058860.1, AK077571.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849383, SAMN01164134
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-602               BC058860.1         16-617
            603-1878            AK077571.1         278-1553
            1879-1900           BC058860.1         1894-1915
FEATURES             Location/Qualifiers
     source          1..1900
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="14"
                     /map="14 33.31 cM"
     gene            1..1900
                     /gene="Tdh"
                     /note="L-threonine dehydrogenase"
                     /db_xref="GeneID:58865"
                     /db_xref="MGI:MGI:1926231"
     exon            1..383
                     /gene="Tdh"
                     /inference="alignment:Splign:2.1.0"
     exon            384..583
                     /gene="Tdh"
                     /inference="alignment:Splign:2.1.0"
     CDS             400..1521
                     /gene="Tdh"
                     /EC_number="1.1.1.103"
                     /note="L-threonine 3-dehydrogenase, mitochondrial"
                     /codon_start=1
                     /product="L-threonine 3-dehydrogenase, mitochondrial
                     precursor"
                     /protein_id="NP_067455.5"
                     /db_xref="CCDS:CCDS27202.1"
                     /db_xref="GeneID:58865"
                     /db_xref="MGI:MGI:1926231"
                     /translation="MLFLGMLKQVVNGTAQSKASSCRKLVLPLKFLGTSQHRIPADAN
                     FHSTSISEAEPPRVLITGGLGQLGVGLANLLRKRFGKDNVILSDIRKPPAHVFHSGPF
                     VYANILDYKSLREIVVNHRISWLFHYSALLSAVGEANVSLARDVNITGLHNVLDVAAE
                     YNVRLFVPSTIGAFGPTSPRNPAPDLCIQRPRTIYGVSKVHTELMGEYYYYRYGLDFR
                     CLRYPGIISADSQPGGGTTDYAVQIFHAAAKNGTFECNLEAGTRLPMMYISDCLRATL
                     EVMEAPAERLSMRTYNISAMSFTPEELAQALRKHAPDFQITYCVDPLRQAIAESWPMI
                     LDDSNARKDWGWKHDFDLPELVATMLNFHGVSTRVAQVN"
     exon            584..626
                     /gene="Tdh"
                     /inference="alignment:Splign:2.1.0"
     exon            627..697
                     /gene="Tdh"
                     /inference="alignment:Splign:2.1.0"
     exon            698..847
                     /gene="Tdh"
                     /inference="alignment:Splign:2.1.0"
     exon            848..1020
                     /gene="Tdh"
                     /inference="alignment:Splign:2.1.0"
     exon            1021..1111
                     /gene="Tdh"
                     /inference="alignment:Splign:2.1.0"
     exon            1112..1381
                     /gene="Tdh"
                     /inference="alignment:Splign:2.1.0"
     exon            1382..1881
                     /gene="Tdh"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1749..1754
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Tdh"
                     /note="hexamer: ATTAAA"
     polyA_site      1770
                     /gene="Tdh"
                     /note="major polyA site"
     regulatory      1859..1864
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Tdh"
                     /note="hexamer: ATTAAA"
     polyA_site      1881
                     /gene="Tdh"
                     /note="major polyA site"
ORIGIN      
        1 ttaacatcag tgttcccagg aaccagaaac gactaagttc ctagttcttg gcggaggtgc
       61 caccagacac aggtccatag cagatctttc cagctgtggc ttgcaagtcc caaattctaa
      121 ccccagtcaa ttgtggaagg ggtgtggtct cgtcttggtg cagccacaag gtcagctccg
      181 tccgagatgt aggtgtgggc ttcccacaag cgtcctgggg cggaggtgag gtgaggtgag
      241 gtaggaggga ggcacggctc ccgcggcggc gaggcgggtc cagagcagac tgaagataaa
      301 aggcagaacc aaactcttga ctccgcacgt gctgctccgg ctcccgcgtg gcgttctcag
      361 catccagtct tctgtgtctg cagggtatca gaagaacaga tgctattcct tgggatgcta
      421 aaacaggtgg tcaatgggac tgcacagagc aaagcctcta gctgccggaa gcttgtcttg
      481 cccttgaagt ttctaggcac ctcccagcac cggatcccag ccgatgccaa cttccactcc
      541 acctcgatct cggaagctga gcctcctcgg gtcttgatca cagggggtct tggccagctg
      601 ggagtaggac ttgctaacct cctgaggaaa cgatttggga aggacaatgt gatcctatca
      661 gacataagga agccccctgc tcatgtcttc cacagtggcc cgtttgttta tgccaacatc
      721 ttggattaca agagcctccg tgagatcgtg gtgaaccacc gtatcagctg gctgtttcac
      781 tacagtgccc tgctgagtgc cgtgggagag gcaaatgtgt ccttagccag agatgtgaat
      841 ataactggat tgcataacgt tctagatgtt gcagctgaat acaacgtgag attgtttgtg
      901 cccagcacga ttggagcctt tggacctacc tctccccgga accctgcacc cgatctctgt
      961 attcaaaggc ctaggaccat ctatggggta tccaaggtcc acacagagct catgggagaa
     1021 tactattatt accggtacgg gttagatttt cggtgcctgc gttatcctgg aatcatttct
     1081 gcagattccc agcctggagg aggaacaact gactacgccg tccagatttt ccacgctgca
     1141 gcaaagaacg gcacattcga gtgcaacctc gaggctggca ccaggctccc aatgatgtac
     1201 atcagtgact gcctcagggc caccctggag gtcatggaag cccctgccga gcgcctgtcc
     1261 atgagaacct acaacatcag cgccatgagc ttcacccccg aagagctggc ccaggcgctc
     1321 cgcaagcacg cgcctgactt ccagatcacc tactgcgtgg atcccctccg acaggccata
     1381 gcggagagct ggcccatgat tctagatgac agcaatgctc ggaaggactg gggctggaag
     1441 catgactttg atcttccaga gttggtggct acgatgctca acttccacgg cgtcagcacc
     1501 agagttgccc aagtgaacta aagaagagga gcggcccctg tgtcctcaaa gtgcagggcg
     1561 cacggcagtc tctaagttgg agagccagac tgaattctag cctctcccag gggacagctt
     1621 gcgtggggtt ggagttcctt tacacactgc ctttgtcctg tgcctaaatt ggtccaagca
     1681 caaaggccag agaaatgaag tcctgagtgg tggtggctat ctctcgggat ctgcagcatt
     1741 tgtatttaat taaattcaat ttaagtatca ctcctgagac ttattctctt gcccagagac
     1801 caaagattac atgttagggt tttcaaagct gttcttcatg tttaagcctg gaatactaat
     1861 taaaaaaacg atcgcttgca aaaaaaaaaa aaaaaaaaaa
//
