LOCUS       NM_001361126            7122 bp    mRNA    linear   ROD 14-JAN-2025
DEFINITION  Mus musculus transcription factor 4 (Tcf4), transcript variant 3,
            mRNA.
ACCESSION   NM_001361126 XM_006525750
VERSION     NM_001361126.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 7122)
  AUTHORS   Pang,Y., Ruan,X., Liu,W., Hou,L., Yin,B., Shu,P. and Peng,X.
  TITLE     MicroRNA-495 Modulates Neuronal Layer Fate Determination by
            Targeting Tcf4
  JOURNAL   Int J Biol Sci 20 (15), 6207-6221 (2024)
   PUBMED   39664574
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7122)
  AUTHORS   Zhang,Y., Li,D., Cai,Y., Zou,R., Zhang,Y., Deng,X., Wang,Y.,
            Tang,T., Ma,Y., Wu,F. and Xie,Y.
  TITLE     Astrocyte allocation during brain development is controlled by
            Tcf4-mediated fate restriction
  JOURNAL   EMBO J 43 (21), 5114-5140 (2024)
   PUBMED   39300210
REFERENCE   3  (bases 1 to 7122)
  AUTHORS   Gaertner,F., Ishikawa-Ankerhold,H., Stutte,S., Fu,W., Weitz,J.,
            Dueck,A., Nelakuditi,B., Fumagalli,V., van den Heuvel,D., Belz,L.,
            Sobirova,G., Zhang,Z., Titova,A., Navarro,A.M., Pekayvaz,K.,
            Lorenz,M., von Baumgarten,L., Kranich,J., Straub,T., Popper,B.,
            Zheden,V., Kaufmann,W.A., Guo,C., Piontek,G., von Stillfried,S.,
            Boor,P., Colonna,M., Clauss,S., Schulz,C., Brocker,T., Walzog,B.,
            Scheiermann,C., Aird,W.C., Nerlov,C., Stark,K., Petzold,T.,
            Engelhardt,S., Sixt,M., Hauschild,R., Rudelius,M.,
            Oostendorp,R.A.J., Iannacone,M., Heinig,M. and Massberg,S.
  TITLE     Plasmacytoid dendritic cells control homeostasis of
            megakaryopoiesis
  JOURNAL   Nature 631 (8021), 645-653 (2024)
   PUBMED   38987596
REFERENCE   4  (bases 1 to 7122)
  AUTHORS   Espinoza,F., Carrazana,R., Retamal-Fredes,E., Avila,D., Papes,F.,
            Muotri,A.R. and Avila,A.
  TITLE     Tcf4 dysfunction alters dorsal and ventral cortical neurogenesis in
            Pitt-Hopkins syndrome mouse model showing sexual dimorphism
  JOURNAL   Biochim Biophys Acta Mol Basis Dis 1870 (5), 167178 (2024)
   PUBMED   38636614
  REMARK    GeneRIF: Tcf4 dysfunction alters dorsal and ventral cortical
            neurogenesis in Pitt-Hopkins syndrome mouse model showing sexual
            dimorphism.
REFERENCE   5  (bases 1 to 7122)
  AUTHORS   Jiang,Y., Gruszka,D., Zeng,C., Swindell,W.R., Gaskill,C.,
            Sorensen,C., Brown,W., Gangwar,R.S., Tsoi,L.C., Webster,J.,
            Sigurethardottir,S.L., Sarkar,M.K., Uppala,R., Kidder,A., Xing,X.,
            Plazyo,O., Xing,E., Billi,A.C., Maverakis,E., Kahlenberg,J.M.,
            Gudjonsson,J.E. and Ward,N.L.
  TITLE     Suppression of TCF4 promotes a ZC3H12A-mediated self-sustaining
            inflammatory feedback cycle involving IL-17RA/IL-17RE epidermal
            signaling
  JOURNAL   JCI Insight 9 (8), e172764 (2024)
   PUBMED   38470486
  REMARK    GeneRIF: Suppression of TCF4 promotes a ZC3H12A-mediated
            self-sustaining inflammatory feedback cycle involving
            IL-17RA/IL-17RE epidermal signaling.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 7122)
  AUTHORS   Sanz,L., Moscat,J. and Diaz-Meco,M.T.
  TITLE     Molecular characterization of a novel transcription factor that
            controls stromelysin expression
  JOURNAL   Mol Cell Biol 15 (6), 3164-3170 (1995)
   PUBMED   7760812
REFERENCE   7  (bases 1 to 7122)
  AUTHORS   Williamson,C.M., Dutton,E.R., Abbott,C.M., Beechey,C.V., Ball,S.T.
            and Peters,J.
  TITLE     Thirteen genes (Cebpb, E2f1, Tcf4, Cyp24, Pck1, Acra4, Edn3, Kcnb1,
            Mc3r, Ntsr, Cd40, Plcg1 and Rcad) that probably lie in the distal
            imprinting region of mouse chromosome 2 are not monoallelically
            expressed
  JOURNAL   Genet Res 65 (2), 83-93 (1995)
   PUBMED   7781998
REFERENCE   8  (bases 1 to 7122)
  AUTHORS   Zhuang,Y., Soriano,P. and Weintraub,H.
  TITLE     The helix-loop-helix gene E2A is required for B cell formation
  JOURNAL   Cell 79 (5), 875-884 (1994)
   PUBMED   8001124
REFERENCE   9  (bases 1 to 7122)
  AUTHORS   Soosaar,A., Chiaramello,A., Zuber,M.X. and Neuman,T.
  TITLE     Expression of basic-helix-loop-helix transcription factor ME2
            during brain development and in the regions of neuronal plasticity
            in the adult brain
  JOURNAL   Brain Res Mol Brain Res 25 (1-2), 176-180 (1994)
   PUBMED   7984047
REFERENCE   10 (bases 1 to 7122)
  AUTHORS   Nielsen,A.L., Pallisgaard,N., Pedersen,F.S. and Jorgensen,P.
  TITLE     Murine helix-loop-helix transcriptional activator proteins binding
            to the E-box motif of the Akv murine leukemia virus enhancer
            identified by cDNA cloning
  JOURNAL   Mol Cell Biol 12 (8), 3449-3459 (1992)
   PUBMED   1321336
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC163095.6, AC169193.2, AC157576.4 and AC109258.12.
            
            On Mar 2, 2018 this sequence version replaced XM_006525750.3.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: X91753.1, SRR7345562.3518596.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-206               AC163095.6         62765-62970         c
            207-298             AC163095.6         61760-61851         c
            299-371             AC163095.6         59986-60058         c
            372-433             AC169193.2         16653-16714         c
            434-530             AC169193.2         13129-13225         c
            531-592             AC157576.4         103312-103373       c
            593-722             AC157576.4         59175-59304         c
            723-772             AC157576.4         58654-58703         c
            773-878             AC109258.12        55571-55676
            879-1012            AC109258.12        58825-58958
            1013-1145           AC109258.12        64877-65009
            1146-1213           AC109258.12        73216-73283
            1214-1292           AC109258.12        74388-74466
            1293-1369           AC109258.12        76955-77031
            1370-1573           AC109258.12        79741-79944
            1574-1709           AC109258.12        97693-97828
            1710-1860           AC109258.12        99961-100111
            1861-2090           AC109258.12        103563-103792
            2091-2231           AC109258.12        104677-104817
            2232-7122           AC109258.12        105071-109961
FEATURES             Location/Qualifiers
     source          1..7122
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="18"
                     /map="18 43.23 cM"
     gene            1..7122
                     /gene="Tcf4"
                     /gene_synonym="5730422P05Rik; ASP-I2; bHLHb19; E2-2; E2.2;
                     ITF-2; ITF-2b; ITF2; ME2; MITF-2A; MITF-2B; SEF-2; SEF2;
                     SEF2-1; Tcf-4; TFE"
                     /note="transcription factor 4"
                     /db_xref="GeneID:21413"
                     /db_xref="MGI:MGI:98506"
     exon            1..206
                     /gene="Tcf4"
                     /gene_synonym="5730422P05Rik; ASP-I2; bHLHb19; E2-2; E2.2;
                     ITF-2; ITF-2b; ITF2; ME2; MITF-2A; MITF-2B; SEF-2; SEF2;
                     SEF2-1; Tcf-4; TFE"
                     /inference="alignment:Splign:2.1.0"
     exon            207..298
                     /gene="Tcf4"
                     /gene_synonym="5730422P05Rik; ASP-I2; bHLHb19; E2-2; E2.2;
                     ITF-2; ITF-2b; ITF2; ME2; MITF-2A; MITF-2B; SEF-2; SEF2;
                     SEF2-1; Tcf-4; TFE"
                     /inference="alignment:Splign:2.1.0"
     CDS             227..2227
                     /gene="Tcf4"
                     /gene_synonym="5730422P05Rik; ASP-I2; bHLHb19; E2-2; E2.2;
                     ITF-2; ITF-2b; ITF2; ME2; MITF-2A; MITF-2B; SEF-2; SEF2;
                     SEF2-1; Tcf-4; TFE"
                     /note="isoform b is encoded by transcript variant 3;
                     immunoglobulin transcription factor-2; SL3-3 enhancer
                     factor 2; MITF-2; class A helix-loop-helix transcription
                     factor ME2; Transcription factor 4 (Immunoglobulin
                     transcription factor 2) (ITF-2) (MITF-2) (SL3-3 enhancer
                     factor 2) (SEF-2) (Class A helix-loop-helix transcription
                     factor ME2)"
                     /codon_start=1
                     /product="transcription factor 4 isoform b"
                     /protein_id="NP_001348055.1"
                     /db_xref="CCDS:CCDS50315.1"
                     /db_xref="GeneID:21413"
                     /db_xref="MGI:MGI:98506"
                     /translation="MHHQQRMAALGTDKELSDLLDFSAMFSPPVSSGKNGPTSLASGH
                     FTGSNVEDRSSSGSWGTGGHPSPSRNYGDGTPYDHMTSRDLGSHDNLSPPFVNSRIQS
                     KTERGSYSSYGRENVQGCHQQSLLGGDMDMGNPGTLSPTKPGSQYYQYSSNNARRRPL
                     HSSAMEVQTKKVRKVPPGLPSSVYAPSASTADYNRDSPGYPSSKPAASTFPSSFFMQD
                     GHHSSDPWSSSSGMNQPGYGGMLGNSSHIPQSSSYCSLHPHERLSYPSHSSADINSSL
                     PPMSTFHRSGTNHYSTSSCTPPANGTDSIMANRGTGAAGSSQTGDALGKALASIYSPD
                     HTNNSFSSNPSTPVGSPPSLSAGTAVWSRNGGQASSSPNYEGPLHSLQSRIEDRLERL
                     DDAIHVLRNHAVGPSTAVPGGHGDMHGIMGPSHNGAMGSLGSGYGTSLLSANRHSLMV
                     GAHREDGVALRGSHSLLPNQVPVPQLPVQSATSPDLNPPQDPYRGMPPGLQGQSVSSG
                     SSEIKSDDEGDENLQDTKSSEDKKLDDDKKDIKSITSNNDDEDLTPEQKAEREKERRM
                     ANNARERLRVRDINEAFKELGRMVQLHLKSDKPQTKLLILHQAVAVILSLEQQVRERN
                     LNPKAACLKRREEEKVSSEPPPLSLAGPHPGMGDAANHMGQM"
     exon            299..371
                     /gene="Tcf4"
                     /gene_synonym="5730422P05Rik; ASP-I2; bHLHb19; E2-2; E2.2;
                     ITF-2; ITF-2b; ITF2; ME2; MITF-2A; MITF-2B; SEF-2; SEF2;
                     SEF2-1; Tcf-4; TFE"
                     /inference="alignment:Splign:2.1.0"
     exon            372..433
                     /gene="Tcf4"
                     /gene_synonym="5730422P05Rik; ASP-I2; bHLHb19; E2-2; E2.2;
                     ITF-2; ITF-2b; ITF2; ME2; MITF-2A; MITF-2B; SEF-2; SEF2;
                     SEF2-1; Tcf-4; TFE"
                     /inference="alignment:Splign:2.1.0"
     exon            434..530
                     /gene="Tcf4"
                     /gene_synonym="5730422P05Rik; ASP-I2; bHLHb19; E2-2; E2.2;
                     ITF-2; ITF-2b; ITF2; ME2; MITF-2A; MITF-2B; SEF-2; SEF2;
                     SEF2-1; Tcf-4; TFE"
                     /inference="alignment:Splign:2.1.0"
     exon            531..592
                     /gene="Tcf4"
                     /gene_synonym="5730422P05Rik; ASP-I2; bHLHb19; E2-2; E2.2;
                     ITF-2; ITF-2b; ITF2; ME2; MITF-2A; MITF-2B; SEF-2; SEF2;
                     SEF2-1; Tcf-4; TFE"
                     /inference="alignment:Splign:2.1.0"
     exon            593..722
                     /gene="Tcf4"
                     /gene_synonym="5730422P05Rik; ASP-I2; bHLHb19; E2-2; E2.2;
                     ITF-2; ITF-2b; ITF2; ME2; MITF-2A; MITF-2B; SEF-2; SEF2;
                     SEF2-1; Tcf-4; TFE"
                     /inference="alignment:Splign:2.1.0"
     exon            723..772
                     /gene="Tcf4"
                     /gene_synonym="5730422P05Rik; ASP-I2; bHLHb19; E2-2; E2.2;
                     ITF-2; ITF-2b; ITF2; ME2; MITF-2A; MITF-2B; SEF-2; SEF2;
                     SEF2-1; Tcf-4; TFE"
                     /inference="alignment:Splign:2.1.0"
     exon            773..878
                     /gene="Tcf4"
                     /gene_synonym="5730422P05Rik; ASP-I2; bHLHb19; E2-2; E2.2;
                     ITF-2; ITF-2b; ITF2; ME2; MITF-2A; MITF-2B; SEF-2; SEF2;
                     SEF2-1; Tcf-4; TFE"
                     /inference="alignment:Splign:2.1.0"
     exon            879..1012
                     /gene="Tcf4"
                     /gene_synonym="5730422P05Rik; ASP-I2; bHLHb19; E2-2; E2.2;
                     ITF-2; ITF-2b; ITF2; ME2; MITF-2A; MITF-2B; SEF-2; SEF2;
                     SEF2-1; Tcf-4; TFE"
                     /inference="alignment:Splign:2.1.0"
     exon            1013..1145
                     /gene="Tcf4"
                     /gene_synonym="5730422P05Rik; ASP-I2; bHLHb19; E2-2; E2.2;
                     ITF-2; ITF-2b; ITF2; ME2; MITF-2A; MITF-2B; SEF-2; SEF2;
                     SEF2-1; Tcf-4; TFE"
                     /inference="alignment:Splign:2.1.0"
     exon            1146..1213
                     /gene="Tcf4"
                     /gene_synonym="5730422P05Rik; ASP-I2; bHLHb19; E2-2; E2.2;
                     ITF-2; ITF-2b; ITF2; ME2; MITF-2A; MITF-2B; SEF-2; SEF2;
                     SEF2-1; Tcf-4; TFE"
                     /inference="alignment:Splign:2.1.0"
     exon            1214..1292
                     /gene="Tcf4"
                     /gene_synonym="5730422P05Rik; ASP-I2; bHLHb19; E2-2; E2.2;
                     ITF-2; ITF-2b; ITF2; ME2; MITF-2A; MITF-2B; SEF-2; SEF2;
                     SEF2-1; Tcf-4; TFE"
                     /inference="alignment:Splign:2.1.0"
     exon            1293..1369
                     /gene="Tcf4"
                     /gene_synonym="5730422P05Rik; ASP-I2; bHLHb19; E2-2; E2.2;
                     ITF-2; ITF-2b; ITF2; ME2; MITF-2A; MITF-2B; SEF-2; SEF2;
                     SEF2-1; Tcf-4; TFE"
                     /inference="alignment:Splign:2.1.0"
     exon            1370..1573
                     /gene="Tcf4"
                     /gene_synonym="5730422P05Rik; ASP-I2; bHLHb19; E2-2; E2.2;
                     ITF-2; ITF-2b; ITF2; ME2; MITF-2A; MITF-2B; SEF-2; SEF2;
                     SEF2-1; Tcf-4; TFE"
                     /inference="alignment:Splign:2.1.0"
     exon            1574..1709
                     /gene="Tcf4"
                     /gene_synonym="5730422P05Rik; ASP-I2; bHLHb19; E2-2; E2.2;
                     ITF-2; ITF-2b; ITF2; ME2; MITF-2A; MITF-2B; SEF-2; SEF2;
                     SEF2-1; Tcf-4; TFE"
                     /inference="alignment:Splign:2.1.0"
     exon            1710..1860
                     /gene="Tcf4"
                     /gene_synonym="5730422P05Rik; ASP-I2; bHLHb19; E2-2; E2.2;
                     ITF-2; ITF-2b; ITF2; ME2; MITF-2A; MITF-2B; SEF-2; SEF2;
                     SEF2-1; Tcf-4; TFE"
                     /inference="alignment:Splign:2.1.0"
     exon            1861..2090
                     /gene="Tcf4"
                     /gene_synonym="5730422P05Rik; ASP-I2; bHLHb19; E2-2; E2.2;
                     ITF-2; ITF-2b; ITF2; ME2; MITF-2A; MITF-2B; SEF-2; SEF2;
                     SEF2-1; Tcf-4; TFE"
                     /inference="alignment:Splign:2.1.0"
     exon            2091..2231
                     /gene="Tcf4"
                     /gene_synonym="5730422P05Rik; ASP-I2; bHLHb19; E2-2; E2.2;
                     ITF-2; ITF-2b; ITF2; ME2; MITF-2A; MITF-2B; SEF-2; SEF2;
                     SEF2-1; Tcf-4; TFE"
                     /inference="alignment:Splign:2.1.0"
     exon            2232..7122
                     /gene="Tcf4"
                     /gene_synonym="5730422P05Rik; ASP-I2; bHLHb19; E2-2; E2.2;
                     ITF-2; ITF-2b; ITF2; ME2; MITF-2A; MITF-2B; SEF-2; SEF2;
                     SEF2-1; Tcf-4; TFE"
                     /inference="alignment:Splign:2.1.0"
     regulatory      7058..7063
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Tcf4"
                     /gene_synonym="5730422P05Rik; ASP-I2; bHLHb19; E2-2; E2.2;
                     ITF-2; ITF-2b; ITF2; ME2; MITF-2A; MITF-2B; SEF-2; SEF2;
                     SEF2-1; Tcf-4; TFE"
                     /note="hexamer: AATAAA"
     polyA_site      7080
                     /gene="Tcf4"
                     /gene_synonym="5730422P05Rik; ASP-I2; bHLHb19; E2-2; E2.2;
                     ITF-2; ITF-2b; ITF2; ME2; MITF-2A; MITF-2B; SEF-2; SEF2;
                     SEF2-1; Tcf-4; TFE"
                     /note="major polyA site"
ORIGIN      
        1 ggtgttaaga gtcagggatc ttggctgtgt gtctgcggat cggcggcggc ggcggcggcg
       61 gcggcggcgg cagcggcggt ggggaagccg aagcgggcgc gggcgccggc cgttggagga
      121 ggtggtggtg gcggaggcag ggggatagac atgaccgccg cctgggcgcc ggcgctgcac
      181 cgggagcccc tcgtcgcgcg cgggcggttt gtgtgatttt gctaaaatgc atcaccaaca
      241 gcgaatggct gccttaggga cggacaaaga gctgagtgat ttactggatt tcagtgcgat
      301 gttttcgcct cctgtaagca gtgggaaaaa tggaccaact tctttggcga gtggacattt
      361 cactggctca aatgtagaag acagaagtag ctcagggtcc tggggaactg gaggccatcc
      421 aagcccgtcc aggaactatg gagatgggac tccctatgac cacatgacta gcagggatct
      481 tgggtcacat gacaatctct ctccaccttt tgtcaattcc agaatacaaa gtaaaacaga
      541 aaggggctca tactcatctt atgggagaga aaacgttcag ggttgccacc agcagagtct
      601 cctcggaggg gacatggata tgggcaatcc aggaaccctt tcgcccacca aacctggctc
      661 ccagtactat cagtattcaa gcaataatgc ccgccggagg cctcttcaca gtagtgccat
      721 ggaggtacag acaaagaaag tccgaaaagt tcctccgggt ttgccgtctt cagtctacgc
      781 tccttcagcc agcactgccg actacaacag ggactcgcca ggctatcctt cctccaagcc
      841 agcagccagc actttcccta gctccttctt catgcaagat ggccatcaca gcagcgaccc
      901 ttggagctcc tccagcggga tgaatcagcc cggctacgga gggatgctgg gcaattcttc
      961 tcatatccca cagtccagca gctactgtag cctgcatcca catgaacgtt tgagctatcc
     1021 atcccactcc tcggcagaca tcaactccag tcttcctccg atgtccacgt tccatcgtag
     1081 tggcacaaac cattacagca cctcttcctg cacaccccct gccaacggaa cagacagtat
     1141 aatggcaaac agaggaactg gggcagcagg cagctcgcag actggagacg ctctagggaa
     1201 agccctagct tcgatctatt ctcctgacca cacgaacaac agcttttcct ccaatccttc
     1261 aactcctgtg ggctcccctc cttcactctc agcaggcaca gctgtttggt ctagaaatgg
     1321 aggacaggcc tcgtcatctc ccaattatga aggacccttg cactcactgc aaagccgaat
     1381 cgaagaccgt ttggaaagac tggacgatgc gattcatgtt ctccggaacc acgcagtggg
     1441 cccgtccaca gctgtgcctg gtggccatgg ggacatgcat gggatcatgg gaccctccca
     1501 caacggagcg atgggtagcc tgggctcagg gtacggaact agtcttctct cagccaacag
     1561 acactcgctc atggttgggg cccaccgtga agatggcgtg gctctgagag gcagccattc
     1621 tctcctgcca aaccaggttc cggtcccaca acttccggtc cagtctgcaa cttcccctga
     1681 cttgaaccca ccccaagacc cttacagagg gatgccacca ggcctccagg gccagagcgt
     1741 gtcttctggt agctctgaga tcaaatccga cgacgagggc gatgagaacc tgcaagacac
     1801 aaaatcttct gaggacaaga aattagatga cgacaagaag gatatcaaat caattactag
     1861 caataacgat gatgaggacc tgaccccaga gcagaaggct gagcgcgaga aggaacggag
     1921 gatggccaat aatgcccgtg agcgcctgag ggtccgagat atcaacgagg ctttcaagga
     1981 gcttggccgt atggtgcagc tccacctgaa gagcgacaag ccccagacca agctcctgat
     2041 tctccaccag gccgtggctg tcatcctcag cctggagcag caagttcgag aaaggaatct
     2101 gaacccgaaa gctgcctgtc tgaaaagaag ggaggaagag aaggtgtcct cagagcctcc
     2161 cccactctcc ttggctggcc cacaccctgg gatgggagac gcagcgaatc acatgggaca
     2221 gatgtgaaaa ggtccaagtt gctaccttgc ttcattaaac aagagaccac ttccttaaca
     2281 gctgtattac cctaaaccca cataaacact gctccttaac cccgtttttt tttgtaatat
     2341 aagacaagtc tgagtagtta tgaatcgcag acgcaagagg tttcagcatt cccaattatc
     2401 aaaaaacaga aaaacaaaca aaaaaatgaa tgaaagaaag aaagaaagaa aaaaatgcaa
     2461 cttgagggac gacttcttta acatatcact ctgaatgtgc gaagcggtat gtacaggctg
     2521 agacacagcc cagagactga atggcaatcc tcccacactg tggagcaatg catttgtgcc
     2581 taaacttctt ttggaaaaaa aaaatataat taatttgtaa gtctgaaaaa aatatttaat
     2641 ttaaaaaaaa attgtaaact tgcaataatg aaaaagtgta cttctgaaga aaacgacatg
     2701 aacgtttttg ttggtattca cgtcagctag tgtttctaat taccggatat tgaatagggg
     2761 aagcccggct gccctcgtaa caaaaccagc aaacgtcctg atggcaacga agtgatgaca
     2821 ttagccattc cttagggtag gagggacaga tggatgttat agacctatga caaatatata
     2881 tataaatata tatataaata tatattaaaa atttagtgac tatggtaagc ttttgttgat
     2941 ttgtttcaga cttttttctc ctgtaaaaaa atagtactga ttaacttttt taaaagaaag
     3001 attttactgt aaatatggat tttttttttt gtctgatttt tgtcccttcc cccggtttgt
     3061 tatcgtaacc tgtagtgcca actctgcttc cggaggggca gtgcaggacg aaatgctgac
     3121 cctgaagttg cttctcattc acaaatagta aaaagttgtt tctccagtct tttgggaaca
     3181 caggacttaa aagtcacatc atgtgtagga attacatgca gcattgcccg ggcgaggcaa
     3241 aaagcgtttg tctggcttgt ggcgctgccc ttgttaccct cccctgggat tttcagaggt
     3301 acacggttag aatgctacaa tgttaccact gtgccttcca atgtttatat catcggaaac
     3361 ataacataat caaagtggct gtgatttaac aaaaaaaacg attcaagtgt tacctacctg
     3421 tgtagccgaa gtagtgtgca gtgaccgaga cgtttctgaa tacatggtca gatttttttt
     3481 ggaaaaaata caaaaattaa aaaaaaaaaa aaaaaagaaa aaaagaaaaa aaaactaaaa
     3541 aagaaagtca agttcaaaaa ccgtcaaatg agaaaattgc aaggagtgtg acagagctga
     3601 ttgatttttg ttgctttctt gatttttttt tcaaaatggg tttactaaaa agtagatgac
     3661 ttacctgcct cctccttcgt ctgaaaaaaa aaaaaaatgc caaccaatca tgcagcatta
     3721 taacaagcct tataagccct aaagcattaa gttgcacttt tgtgaggagg ggtaacgcag
     3781 tattctctct ggccagtatg agtgaagttt atacttaaca tttgatagaa acatagatca
     3841 aactacggca cagcaactca tcagatagct agcgttgact ctgggcacaa ttgaaccaat
     3901 tcccatcgta agtctttcca acaatggact ttggtgtgta gtgcagtgaa cacataggac
     3961 tcctacgtaa gcatttgtca gcatcctttt gtctctaact cggttctgtt ttgacagcca
     4021 cacaatcttg gcatgtatta aggaaaaaaa aaatccctgt tgaagtagtt tttccaccta
     4081 tcagcactga gtaaatgcca taactccgcg gaaatggtct aaatacccca tttgttgtcc
     4141 tgaactacca gttacatagt gacgaaacac atctgtaaat tttatgcaac caatggcaaa
     4201 cctatcatta ttttgaaact gtgtatgtaa aagttatatt tttacatgta gactcttgtt
     4261 attatgtgtt ttaatacatt gtatcagttt tttgtttttt tttaaactgt gtggttttaa
     4321 aaagtcattt aaatgaaata gtgagctaca agaatctgaa atttatgttc atttctgaaa
     4381 atgtaagaac aaataagata gttaccacgt ggtcaccttt tacaaaccca tgaacatttt
     4441 gattagctgt gtgcatgtgt gtgcgtgtgt gctcttgcgc gtgtgtgtgt gtgtgtgtgt
     4501 gtgtgtatgt gtgtgtgttg aaaattgtaa atatgttcag tagcgataaa actaaaatgc
     4561 tttgatttgt tgagaagttc ctaaaatgtg gaggtggatt gaaaatgtag gggaatagca
     4621 ggaatcaaat ctcataaaaa gttcttcggg gactttgctg tgatgcggtg gcacaaaggt
     4681 gctccaagaa gggcaaagaa gaaagacagt aacttcccct cctccctgaa cgatggaaaa
     4741 catgtgtacg tggtctctca ccatgttaaa gattttcttt ccgtgataca ttctgcactc
     4801 attttgtata gtctgccaag gcgggtatcc ctaggaacaa tattattata taggaaacag
     4861 gtatcgtccg atcacattca ggagaagcgt atagaaaaga atatggtgtt tactctttag
     4921 ggaactggaa atcctccccg cattgatgga tatcttcaga gtggcacttt tgatacatgt
     4981 tatcataaag gtgcttacta aagcagaatc aaagtttttc aactctgtaa acaaagcaaa
     5041 aaattaaatt tttaaattaa atcatttgag attttttttt tcaattggtg cttttatatt
     5101 ttgttctcac tcggacagag aaaaagctgc aatttcatgt tctcaccagc tttgatgtat
     5161 tcattacttg gtaatgtaat atggctattg tcaaattcct tttggaaata agcaaaagac
     5221 tccccaaagg ccagcagcag ctgctggcta ctttctgctt ggtgcggaaa cctgatctga
     5281 ccctctttgt agtaatcagg tgtatctcca gttttaaaaa gaaggagaaa ggaaatgtgg
     5341 ccgttttaac gtgttggttt tgttttgttt tgttttgttt tgttttcctt gttgttattt
     5401 tgtaattatc aaacccaggt aagatattgg tattcctgca ctggattttt gaaagaaact
     5461 tagcagaagt caggattaaa atactacaca aacatttcat aagtgttcat cctatactag
     5521 acatacacga aggcggtgag gcttaggtgc aggatcggtt taagagactt tgaaagcaac
     5581 ttggaacagt tgatccacct ccacattaaa gtaaattatg aatatgcaga attagggatc
     5641 tgtccatctt ggagttgtta ctctttttgt cttctgtatg gcagtgatga acactaatgc
     5701 tttgacagct ttcagcccac ccagtggtcc aactgattcc aattcagtct tccgattcct
     5761 ggttttgttt tgtctttcca cttttttttt cctatcttga ttttattgtt attgttattg
     5821 ttattattat tattattatt attattaata ttcatgatgt ttgttttgat gagggattgg
     5881 gaagtgggag ggagtcgaac tgagactagg ggctgagagg attttttttt ttccatcttt
     5941 gcatccaaca ggcagaatat ggtctgtgtc caaaacggaa cttaagtcag gaaggaaacc
     6001 attcagcata aacaagcaca acatgtagtc tgccggctga ctggaagcat aaataaataa
     6061 ataaataaca catacggagg tgaagatgga atgtgctgga ttccaagaca atggggcacc
     6121 aaggcctgag ggcctcctct tgactttgct ttggttatgt ttgttttctt tagagatgtc
     6181 ttttctcatg ggacttgaag tgactcatct ctgtgcagta ctggtttcgc catatgctca
     6241 tttcaagtat tatagacata tgtaatggtg aaatatatga actgtggcct ttttcattct
     6301 tgttacttgt gatgcaatta agtgaagata agaaaaaaaa aaagcagaga tttaccatgt
     6361 atcagtgcct ggctttttgt tataaagctt cgtctgtcta gtgctctttt tgctataaaa
     6421 aatagactgt agtacaccct agtaggaaaa aaaaactaaa tttaaaaata aaaaaatata
     6481 tttggcttat ttttcgcagg agtaatcctt ttataccatg gatattacaa aaaaaaatgt
     6541 cagattctga gtatttcttc tttgtagatt tttggaatca ttatgagtaa aagtttgtta
     6601 ctttattttg ctatttaaaa gatgttattt taccatgtgt tactgaaagg aaactgtatg
     6661 gtagagcttc tgtttgtttt tgttttcagg ttttttttgt ttgtttgttt ttagttgtag
     6721 gtcgcagtat gaactttttt tttgtttctc gttatgtttt gttttgtttt tgcgactgta
     6781 cacatagctg cagcattaaa aacttttaaa aaattgttta aaaaaaaaga aaaaaaggga
     6841 aaacgtttta aaaaaaaaga taaacagtta caccttgttt tcaatgtgtg gctgagtgcc
     6901 tcaatttttt catgtttttg gtgtatttct gatttgtaga agtgtccaaa caggttgtgt
     6961 gccggacttc cttcaagagg cccacagccc agcttggtct agacctgttc ccatctgtag
     7021 ttactcgatg aagtcatgta catgaccgtt ctgtagcaat aaatgtgcca tttttataaa
     7081 ctgtctctga cacttttttc atttcacctc tcgtgcacat ag
//
